LOCUS       NZ_CP103498             2511 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain 70 plasmid pMB9539_4, complete
            sequence.
ACCESSION   NZ_CP103498
VERSION     NZ_CP103498.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173982
            Assembly: GCF_024917775.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 163x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024917775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 06:52:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,746
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 42 of 68
            Pseudo Genes (internal stop)      :: 14 of 68
            Pseudo Genes (multiple problems)  :: 14 of 68
            Pseudo Genes (short protein)      :: 1 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103498.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="70"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMB9539_4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-12-07"
                     /collected_by="MDACC"
                     /genotype="ST106"
     gene            558..701
                     /locus_tag="M5T15_RS23700"
                     /old_locus_tag="M5T15_23700"
     CDS             558..701
                     /locus_tag="M5T15_RS23700"
                     /old_locus_tag="M5T15_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1129..1356
                     /locus_tag="M5T15_RS23705"
                     /old_locus_tag="M5T15_23705"
     CDS             1129..1356
                     /locus_tag="M5T15_RS23705"
                     /old_locus_tag="M5T15_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1723..1908
                     /locus_tag="M5T15_RS23710"
                     /old_locus_tag="M5T15_23710"
     CDS             1723..1908
                     /locus_tag="M5T15_RS23710"
                     /old_locus_tag="M5T15_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1956..2035)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          163..723
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 cggtacaggc gggttccggg cgcagcgccg gaccagtcac tcagcgctag cggagtgtat
       61 actggctaaa atgtaacgcc gcagtaacgc atagcataac tgctacataa gacaggcgca
      121 gaaaatgaaa gaaaacgcgt caggagaaaa tgtaatacag gatatattcc gcttcctcgc
      181 tcactcactc gctgcgctcg gtcgttcggc tgcggcgagc ggtacgggct tacgggtgga
      241 gcagaaattt cctaggggat gccagctagg ggatgccagg aagatactta gcgaagaagg
      301 cataaggccg cgccgaagcc gtttttccat aggctccgcc cccctgacga gcatcacgaa
      361 atctgacgct caaatcagtg gtggcgaaac ccgatgcggc ttaaagatac caggcgtttc
      421 cccctgatgg ctccctcgtg ctcccccgca ttcctgcctt tcggtttacc ggtgtcactc
      481 cgctgttatg gccgcggttt ctcattccac gcctgacact cggttccggg aaggcagttc
      541 gctccaggat ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg
      601 gtaactatcg tcttgagtcc aacccggaaa gacacgtaaa aacgccactg gcagcggcca
      661 ctggtaaggg gtatacaaga ttactggtgc agagttcttg aagttgacgc ctgactgcgg
      721 ctacactaga aggacaaatt tggtggcagc ggtcttgtac accctgttac cacggttcag
      781 aaacttgccg gtttgctgaa ccttcgaaaa accaccttgc cgggtggttt tttcgtttac
      841 agggcaagag attacgcgcg aaaaaaaagg atctcaagaa gatcctttta taaattagac
      901 atccaacctc acaaagagga atacttaaat catcttggta tatgtatcgg tcggattaca
      961 tcagatataa gcccgcactg ccccctcccc gccagacacc acactttaac cataacacct
     1021 cgcacctaac tcgatatgca gcccaccatc aagcctcttt tcacataaaa aatgacctat
     1081 agttagatgg ttagtgacca aaactgatat ggataaaagg agctggcttt gaaaaagatt
     1141 tttgtttccg tgttcgcagc ggcagttgcg ctttcagccc tcaccggctg cacgcgcaca
     1201 agctatgcca tacacaccaa tgacgggcgc actattgtca gcgacggcaa acctaaagaa
     1261 tcagactcag gcctgcttgg ctatacagac gcgaatggtg tcaaacagca gatcaataaa
     1321 accgacgtta aagaagtctc cgaaattccg cactgagcat cttctccgaa aaaggccgct
     1381 aatgtggcct ttttctttac ctggggttct ccgccaaaat cccagcaaat tgcaccacca
     1441 aagctaaaag cttccagggt tgtctaaaaa gcacaagaca ttagaggaag tgcggttttt
     1501 ataatcaagc cccccaagaa tttaccataa catccgttgc ccgcaccgcc tcagacgcac
     1561 tcagcgcttt tctgacggaa ccatgaccaa aaaccacaac aaaccacccc ggaacccgcc
     1621 agaaacgctc ctgttaaatt ttaacggcat gcatgactat gcaccagaat gacggcatgc
     1681 tcttttcacg caaaaatcat caccagacgg ggaaaatcac cagtgaccag acaggaatcc
     1741 gccgccctca acatggccaa atttatccgc tcacagacac tcctcctcct cgagcggctc
     1801 gaacagatgg atctggatga ggctgccggc tgctgtgagc acctgcacga tcaggccgaa
     1861 gcactttaca ccatgctgaa cgcacagaca ggcgaggaag atgcgtgaaa atcggtgaac
     1921 gggtgcgcaa ttcagtgcgc ggccgtgagg cgatggcggg gtgtcggggc gcagccctga
     1981 cctgggtatt tgtgatgccg gcgcgtgcgc ggtattacaa atacacatcc tgtcctgagt
     2041 ggttccccgg gaacaggcaa aaaaaaaccg gggagctgcc cggaaggtaa gtcaagttag
     2101 attagattac tttcactcat ccataacagc atcatgcaac gacgaccatc gtccgtgacg
     2161 gccgcctcgt ttaagtatgg acagaaatac agaaaatgct caggacgaaa tgtaatgaat
     2221 gcgaacggat tcaagaaatt cgagcatgac cgtccttacg gccggttcag tttcagacaa
     2281 aatctgccgg tatgcatcca gcatcatggc tccggcatcc ccccggcacg ccggagccag
     2341 cccgaaacaa cagacacaaa caggtgtcgc tcatcatcaa gagtcatcag gtctccggaa
     2401 gaaaaaccaa acatggctta cctcctgtta acggcaacgc agaagaaagg tttacagggt
     2461 agcgggcagg aaaataaaaa aattgcagtc agcccggaac ggctgctgaa c
//
