LOCUS       NZ_CP103497             4760 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain 70 plasmid pMB9539_3, complete
            sequence.
ACCESSION   NZ_CP103497
VERSION     NZ_CP103497.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173982
            Assembly: GCF_024917775.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 163x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024917775.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 06:52:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,746
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 42 of 68
            Pseudo Genes (internal stop)      :: 14 of 68
            Pseudo Genes (multiple problems)  :: 14 of 68
            Pseudo Genes (short protein)      :: 1 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103497.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="70"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMB9539_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-12-07"
                     /collected_by="MDACC"
                     /genotype="ST106"
     gene            308..769
                     /locus_tag="M5T15_RS23665"
                     /old_locus_tag="M5T15_23665"
     CDS             308..769
                     /locus_tag="M5T15_RS23665"
                     /old_locus_tag="M5T15_23665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(1024..1134)
                     /locus_tag="M5T15_RS23670"
                     /old_locus_tag="M5T15_23670"
     CDS             complement(1024..1134)
                     /locus_tag="M5T15_RS23670"
                     /old_locus_tag="M5T15_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            1778..2101
                     /locus_tag="M5T15_RS23675"
                     /old_locus_tag="M5T15_23675"
     CDS             1778..2101
                     /locus_tag="M5T15_RS23675"
                     /old_locus_tag="M5T15_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2310..>2744
                     /locus_tag="M5T15_RS23680"
                     /old_locus_tag="M5T15_23680"
                     /pseudo=""
     CDS             2310..>2744
                     /locus_tag="M5T15_RS23680"
                     /old_locus_tag="M5T15_23680"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2779..3264
                     /locus_tag="M5T15_RS23685"
                     /old_locus_tag="M5T15_23685"
     CDS             2779..3264
                     /locus_tag="M5T15_RS23685"
                     /old_locus_tag="M5T15_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3268..3483
                     /locus_tag="M5T15_RS23690"
                     /old_locus_tag="M5T15_23690"
     CDS             3268..3483
                     /locus_tag="M5T15_RS23690"
                     /old_locus_tag="M5T15_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3754..4668
                     /gene="lpxO"
                     /locus_tag="M5T15_RS23695"
                     /old_locus_tag="M5T15_23695"
     CDS             3754..4668
                     /gene="lpxO"
                     /locus_tag="M5T15_RS23695"
                     /old_locus_tag="M5T15_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          1491..1674
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(1025..1508)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          2092..3581
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaatctttt tagtaagaaa ggcccgaatc tgacgctcag cgggcctttc gtgaagaagt
       61 gcggcagtag acagatttca tctgatcacg ttcatgtgct gctgtgcaat taagcgtttc
      121 tttcggagct ttcacggtat ttccttcacg aaaccggccg ccattcactc ttgtgcatag
      181 ccatgcagcc gcgcagaaat tatcaggggc gtttttcagg gtttgggccg gggaaaggtg
      241 ccggaaacgg ccctcaggcc gcagaaacgc gctgaggggc tctcaggggg tgcggccaag
      301 aagcgttatg gtaaatacat attatggtta tttattagga gtaactctgg gagagtgggc
      361 tatgggatca gatgttcaat ggcagataga taatggttat ttggcgcaag ttatggggga
      421 tttttcgaca gagtcgccta gctttgaaac gccaatgttt gggcgatatt ttgatacctt
      481 tcaagctgct atacatgttg caaaagcttt cccagggagt acgttgcagc catcagagta
      541 cgaagataaa actgggatgc taatacgtca ttattatgtg gattgtcatc cgtattatga
      601 tcgactccct cctgaaaaaa gcgataattt tcagtatgat gagtaccctt ctgctattca
      661 tgtggctgcg tgtattgatc aagctagcaa agaactcgac gaactattgt ttggtggtgg
      721 atatggggaa aatggtgagg ggcatgcaag cccgataatt ttatattagt gttaggggcg
      781 gagggatatt atccaatttg taaaatgatc ttcttgagat cgttttgctc tgcgcgtaat
      841 ctcttgctct gaaaacgaaa aaaccacctg gggaggtggt ttttcgaagg ttcagtaagt
      901 tggggaactt ctgaaccgtg gtaacagtct tgtgcgagac gtgtcaccaa atctgtcctt
      961 ccagtgtagc cgtagtccgg ccaccacttc aagaactctc gatacatctc tcgcacatcc
     1021 tgcttaccag tggctaccgc caatggcgat tttgtcgtgt ctttccgggt tggactcaag
     1081 ttgatagtta ccggaatggg cgtggtagtc ggactgaacg ggggggtcgt gcatacagtc
     1141 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     1201 gccacataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     1261 ggagctacca gggggaaacg cctgggatct ttatagtcct gtcgggtttc gccgccactg
     1321 atttgagcgt catatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggcaa
     1381 cgccgcatcc ttttcccagc ccttcctgat ttgagcgcca tctctttatc ccgccattcg
     1441 tgagccggta acgcccgccg cagtctcacg gcaggagcgt agcgaccgag tgagcgagga
     1501 ggcgtcattt catcgtcccc agcatttcgc acttacgccc cacagacgca ttttttacct
     1561 gccacatgaa gcacttccct gaattcccac ttcgatttac catattaagc cagtatacac
     1621 tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctaa aaccactgac
     1681 gcgcctgcgg cttgtccaac accgcgccga ccgggaaaag atttcccgcc cgtcccggcg
     1741 ttatcaggag gccggattgg cagacaaacg cagcaaaatg ctcacgatgt gggtgaccga
     1801 ggatgagcac cggcgtctgc tggagcgctg cgacggtaaa cagcttgcag cctggatgcg
     1861 ccagacgtgc ctggacgaga aacccgcccg cgccggcaag cttccctcga tctcgccggc
     1921 gctgcttcgt cagcttgccg gcatggggaa caaccttaac cagattgccc ggcaggttaa
     1981 cgccggtggt ggcagcgggc acgaccgcgt gcaggtcgtc gccgcgctga tggccataga
     2041 tgccggactc gagcggctgc ggcatgccgt gctggaaaag ggtgcagacg atgatcgtta
     2101 agtttcaccc gcgagggcgc ggcggtggcg ccggtccggt ggattatctg ctggggaaag
     2161 accgacagcg cgagggcgcc accgttctgc aggggaagcc ggaagaagtc agggagctta
     2221 tcgacgcctc accctacgca aaaaagtaca catccggcgt cctgtccttt gccgaaaagg
     2281 atttaccgcc cgggcagcgc gaaaagctga tggcgagctt tgaacgggtt ctgatgcccg
     2341 gactcgacaa agaccagtac agcgtgctgt gggttgagca ccaggataag gggcggctgg
     2401 agctgaactt cctgatcccg aacacggagc tgctgactgg caagcgtctc cagccgtatt
     2461 acgaccgcgc cgaccgtccg cgcattgatg cctggcagac catcgtgaac ggcaggctgg
     2521 ggctgcatga cccgaacgcg cctgaaaacc ggcgggcgct ggtcacgccg tccgcgctgc
     2581 cggaaacgaa gcaggaagcc gctcaggcaa ttacacgggg tttactggcc ttggcctcgt
     2641 ccggggagct gaaaacccgc caggacgtca ctgaggcgct ggaaagcgca ggttttgagg
     2701 tggtacgcac cacaaaaagc agcatcagca ttgccgaccc ggacgggggg cgaaacatcc
     2761 gactgaaggg agccatctat gaacagtctt ttaacgctgg cgaaggactt agagcagaaa
     2821 tcgaaagcgc agcagcagag taccggcgag atgctgaaag ccgcattcag cgagcacgag
     2881 aagtctgtca gagcggaact gagcgaaagc gagaagagaa tcagcgccgc catcctcgac
     2941 cacgaccgga agctgtcctc agccatgagc cagcgtacga aagggatgct gcgcatggtc
     3001 agccagacgt ggctgaccat cgtcctggtc tccgtgctgc tgatcgcctc gaacgcggcc
     3061 attctgtggt ggcagagtca gcagatactc gacaattacg tgagcatccg ggagcagaag
     3121 agcacgcagg cgatgctgtc cgagcggaac agcggcgtgc agctctcgac ctgcggcgag
     3181 cagagacgcc gctgcgtgag ggtgaacccg gaagcgggac ggttcggaga ggattcgagc
     3241 tggatgatac tggcggggaa atagcacatg acagagctgg aaaaacagtt gctgagcgca
     3301 ttagagcagc tacagcagga ctactcgaaa aggctggacg agtgggagag cgccttcgcg
     3361 gaatggcgga cgatgtctgg tcttatgcaa cgggagaacg cggcgctgag cgagcgcgtc
     3421 acgggcttga gcaggcaggt gcagagtttg agcgatcagc tgcgccggtt gtcgagcggc
     3481 tgaatgccgt cgagctgcag caggtgcggg aggcgcggca tcagaaagcg ctggagctcg
     3541 aacgatcaca gcgacagcgg gtatatcacg gtccgtccct ttgattcatt tcagcataat
     3601 tcctgctgaa atcctctctg cctgtgtagt agatatgttc tggtgaagct tcgattatac
     3661 tattgataaa tagcatttag attataattt aagtgcattc attatgttat ctttaagaga
     3721 tttttattat tggcgtgaac aaggaatata cacatgctgt cacttattat tatttttgtt
     3781 tttcttatca gtgtattgta tgcgcattct cgcggtaaaa taaaacaaaa gttttccagg
     3841 cagttatttg atcattcaac atttatggct ccgataaata tgtttatgac aggcttttcg
     3901 aagctgccgt caaagcaggc atggtttccc ccagaaacat ttcctgaact tcaaccatta
     3961 attgataact ggcaggttat aagagacgaa gctgttaatt tgcaagggca cattaaatca
     4021 tctgaaaaaa ataatgatgc aggatttaat acatttttta agcgcggttg gaagcgcttt
     4081 tacctcaagt ggtatggaga tgctcatcca tcagcggagc ggctttgccc agtaacaacc
     4141 caactggtta gctctattcc ctgtattaaa gcagcgatgt ttgctgaatt gccttcagga
     4201 agccatttag gaaagcatcg tgacccttat gccggttcag ttcgttatca tttaggatta
     4261 tcaacaccaa atgatgaacg gtgttttatt gaagttgatg gtgtacgccg cagttggcat
     4321 gatggtgaag tgataatttt tgacgaaact tttgttcact gggctcagaa tgataccgag
     4381 cagacacgta ttattttgtt ctgtgatata gaaaggccga tgaagtggcg gtggtcacaa
     4441 aaaataaata ggtggatagg tggggtgcta atgtcagcgg cagcatcacc caacgataat
     4501 aatgacagga ctggtgctat aaatcattta tttaaatatg ttaatgctat tcgtgatgca
     4561 ggtcagaagc taaaaaaaag gaatcgaaaa ctatattatt tgattaaaaa tggagttatt
     4621 tctttgattt ttgtttggct tgctgtctca gtgtttagat ggctgtgatt tgtttttttc
     4681 tattgagtca tacataaatt tgggtcttct atgctcaatg tatggttata gataaaaatc
     4741 gcctgctaaa aaattctatt
//
