LOCUS       NZ_CP103493             2772 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain 5463
            plasmid pMB9544_6, complete sequence.
ACCESSION   NZ_CP103493
VERSION     NZ_CP103493.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174098
            Assembly: GCF_024917695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 278x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 05:55:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,465
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,179
            CDSs (with protein)               :: 5,179
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 70 of 160
            Pseudo Genes (incomplete)         :: 90 of 160
            Pseudo Genes (internal stop)      :: 25 of 160
            Pseudo Genes (multiple problems)  :: 21 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103493.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="5463"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pMB9544_6"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-12-12"
                     /collected_by="MDACC"
                     /genotype="ST571"
     gene            1210..2394
                     /locus_tag="M5T26_RS27265"
                     /old_locus_tag="M5T26_27265"
     CDS             1210..2394
                     /locus_tag="M5T26_RS27265"
                     /old_locus_tag="M5T26_27265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_087703907.1"
                     /translation="MELTKKQKQIAEARLMIAHQQSIDSGTFIEIQIDKMIKGIIGKVK
                     TNANDASKEQYRKHYRSAIERHPDASFHELLMQAGTASSFDKRRTAFRFCITEEIQNLR
                     RKSEKAMRSKDYEAGKKLIQEAFKLAIKFEDEFLSDNRILFNDVKSQDGFKKVSFSKKK
                     GLKKAPDPDVVLNGLELKKNLYDRHSLAFAVISVFGIRPAEMQKGVKLKFIDGMIFAQV
                     KGAKVSDDKGHKYRAIGVSVRDGNKCDQVIMEAIYKNDGEVLATQTKSDYDSLRKYFNN
                     HYNGTSLYTYRHQVASDLKKAGVSKTQIAEVMGHRTTESQVHYGYARSSRGGRSMVAKG
                     SNEVISSPDIKDYLKPKPAPAGGGGTGSKIAKAKNNLSQGVQASYPKLTPRGTK"
                     /name="site-specific integrase"
ORIGIN
        1 gtgattgttc gggtaggtgt tggtgttggt ctaaacgggg agtgttgcaa aagaccagac
       61 cgggaacgag tcactaaggc gcttggtaag gattcaggcc agctatgaga aagcccagcc
      121 ataaatgggg ttaggattct gcaagtagtg ctatgtggcg acagcggggg tgcaacaggg
      181 tgccagttgg tgggaaactg ccttcagctc taagtcgctg ctctttgggt gaagctcaaa
      241 aaagaagctt taccaaagga tcgtcaactt gaattcgtga tgaattcgag gaggcgaaac
      301 tttgggtgaa gcgccctttt tctgggggct gaacatcctt tatcccttat ggcgtagcac
      361 aggccgaagg cttgatcaga gttagtaata ctcgatcttt tatttgagag agagtaagat
      421 cggcgcagtt tcaactccat gaaaaatcta gagtttggta gaaatccttc gataaatgac
      481 gctttttttt cgagcacttt acgaagaata ggcgaaggtc agatcaattt aaaaatggtc
      541 tgtaactatc aaactttatt ggattttttg atatggaaaa caacacaaaa gaagttctcc
      601 actgtgcgta ctgtgatact gagctgacga agggagtgca tgtttgtcga acgtgccttg
      661 ctacggtgaa gtatggaacg ccgcccggtt ggttagtctt cttggtgtgg ttagtgggga
      721 tggtcggcgg tgcattgtta gcaatgtata cacagtcagg atcatccatg attttaggat
      781 ttgtttttat gggtgtaggt ttcttggtac tgaaaggtat ttatgcaaac cgaataacat
      841 ttcatagaag ataaaaccta aaatattata aggccggata aacccccggc cttttttttt
      901 gaataaatcc cactcgctga cgcgctagaa aataattttt aaaaggtaca tggacaagcc
      961 aaaaataatt gattattaat tacatataca ttattgggaa cgtaagattt taacattatt
     1021 gagaacggcc ctttcgttcg caataatgta ggtgtgtctt tcgttttttc gacagaaaaa
     1081 taagaaagtt gcgggtagca aaacaccttt ttttattctg acttttgcga ttaggagcga
     1141 gcgacttttc gcaataggaa gaataaaaat acagacaaca ccaatgtaaa aataaaaagg
     1201 agatctgaca tggagctaac caaaaagcaa aaacaaatag ctgaagcaag attgatgata
     1261 gcacatcaac aatcaattga tagcggtaca ttcatcgaaa tacaaattga taaaatgatt
     1321 aaaggtatca tcgggaaagt aaaaacgaat gccaacgatg cctcaaaaga gcagtacagg
     1381 aagcactacc gatctgctat agaacgtcac ccggatgcat cctttcatga gcttctgatg
     1441 caagcgggga cggcctctag ctttgataag aggcgtacag ccttcaggtt ttgcatcact
     1501 gaagagatcc agaacttgag acgtaaatct gaaaaggcta tgcgttctaa agattatgaa
     1561 gcaggaaaaa aactaatcca agaagcattt aaacttgcta ttaaatttga agatgaattt
     1621 ttatctgata atagaattct atttaatgat gtaaaaagcc aagatgggtt taaaaaagtt
     1681 tctttcagta agaaaaaagg acttaaaaag gctcctgatc ctgatgttgt tttaaatggt
     1741 ttggagttga aaaagaactt atatgatagg cattcattag cttttgctgt tatttcagta
     1801 tttggtatac gcccggcaga aatgcaaaaa ggcgtaaagc taaagtttat agatggtatg
     1861 atttttgcgc aggttaaagg tgcaaaggta tcggatgata aaggtcataa gtatagagca
     1921 ataggtgttt ctgttcgtga tggtaataaa tgcgatcagg ttattatgga ggccatttat
     1981 aaaaatgatg gtgaggtttt agctactcaa acaaaaagcg attatgacag tttacgcaag
     2041 tattttaata accattataa cgggacttct ctttatacat accgccacca agtagcaagc
     2101 gatttaaaaa aagcgggtgt ctcaaaaact caaatagctg aggttatggg gcatagaact
     2161 actgagtcac aggttcatta tggatacgcc agaagctcaa ggggcgggcg ttctatggta
     2221 gcgaaggggt ctaatgaggt aatcagtagc ccggacatca aagactatct gaaaccaaaa
     2281 ccggctcctg caggtggtgg tggaactgga agcaagatcg caaaggccaa aaataattta
     2341 tctcaggggg ttcaggccag ttacccaaag ctgactcctc gcggaaccaa gtaggaggcg
     2401 ggtaagccgt caaaaactcg ttttttgatg ggttagccgt caatgatttt gacttcaatt
     2461 tttttgatag actattgacg tgtaagcgat ttcggttata acaggtgaca gatacaacaa
     2521 agccaaccga ataggctggc tttgagggac ttaaaaagtt tgcagacttt ttaggtcttg
     2581 taaccaagtt ggttcagaca acgtgattac gatatgtaga tcaaatctat cctacatccg
     2641 cgtaaaagtc aaacctattt ccttacaagt gccaaaaaaa atctagcctt ttcaagtcct
     2701 taagcaccaa tacatttaat aacccattgt atcgggatct acctgtggag ccgcatatgg
     2761 aggcatatgc at
//
