LOCUS       NZ_CP103492             4143 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain 5463
            plasmid pMB9544_5, complete sequence.
ACCESSION   NZ_CP103492
VERSION     NZ_CP103492.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174098
            Assembly: GCF_024917695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 278x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 05:55:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,465
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,179
            CDSs (with protein)               :: 5,179
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 70 of 160
            Pseudo Genes (incomplete)         :: 90 of 160
            Pseudo Genes (internal stop)      :: 25 of 160
            Pseudo Genes (multiple problems)  :: 21 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103492.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="5463"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pMB9544_5"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-12-12"
                     /collected_by="MDACC"
                     /genotype="ST571"
     gene            1..867
                     /locus_tag="M5T26_RS27230"
                     /old_locus_tag="M5T26_27230"
     CDS             1..867
                     /locus_tag="M5T26_RS27230"
                     /old_locus_tag="M5T26_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_045287010.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEKVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKELKATARSIARWTWKNFDSHTFSLIQSNRGLKNKGKTKKTNVKNEMLDI
                     LMSKGVL"
                     /name="replication initiation protein"
     gene            864..1127
                     /locus_tag="M5T26_RS27235"
                     /old_locus_tag="M5T26_27235"
     CDS             864..1127
                     /locus_tag="M5T26_RS27235"
                     /old_locus_tag="M5T26_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(1367..1651)
                     /locus_tag="M5T26_RS27240"
                     /old_locus_tag="M5T26_27240"
     CDS             complement(1367..1651)
                     /locus_tag="M5T26_RS27240"
                     /old_locus_tag="M5T26_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_045287008.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LNSKPEEVNKALLLGLLSKIKELDEEEKRDFTRIGMNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(1644..1979)
                     /locus_tag="M5T26_RS27245"
                     /old_locus_tag="M5T26_27245"
     CDS             complement(1644..1979)
                     /locus_tag="M5T26_RS27245"
                     /old_locus_tag="M5T26_27245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287007.1"
                     /translation="MENIFTVHIEEGFPISSIPALIDLFLRHYHCNNFDIEKALEATRE
                     NIADNLGALTKMKKPKVDIERIMVEDIMSGLKTMVSEMGILELYKIHFEINEMGMSWGV
                     EEEVNNE"
                     /name="hypothetical protein"
     gene            complement(1995..2246)
                     /locus_tag="M5T26_RS27250"
                     /old_locus_tag="M5T26_27250"
     CDS             complement(1995..2246)
                     /locus_tag="M5T26_RS27250"
                     /old_locus_tag="M5T26_27250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_131632043.1"
                     /translation="MEYKNIYKKCCKCRKETEHEREEGYVTEKSLSKDDWEYSPAELAS
                     YMNGEFYDDYEEVLRLFDTCCVCGDKSYGRWSSEELTK"
                     /name="hypothetical protein"
     gene            2484..3644
                     /gene="mobQ"
                     /locus_tag="M5T26_RS27255"
                     /old_locus_tag="M5T26_27255"
     CDS             2484..3644
                     /gene="mobQ"
                     /locus_tag="M5T26_RS27255"
                     /old_locus_tag="M5T26_27255"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_117059963.1"
                     /translation="MAIYHLSFKLCKRSEGKSSVYLAAYQNRNKFTDNRTGAVWNYSKK
                     EGFDGSLILAPPQAPGWLVKSSEALWNEVERTERQKNGQTARYFDVALPVELDDKQKKD
                     LLIKYCQDNFVDEGMIADIAFHDLNSKNPHAHVMLTMKNVGPEGFLTKNREWNDKKCLE
                     AWRQNWEVITNEALKKYGHKSRIDSRSLEEQKEEADRKAKVAKTPQAKAAWIAKSIRLD
                     RTPMTRIHRHNWKTGQKLRKLEQEERKILYKKASLTYHQITKPIEPEQSMKIEKSQIAQ
                     KIKSIFETAKNKLNSKIADFFKPKPKKETPEKFVSSYEIDELGNHILKKEAENWDQKNN
                     DKIKKQVREKKEKEESERLRLKEEDEKLKNSSKPIQQNNNHRKLKR"
                     /name="mobQ"
     gene            complement(3676..3924)
                     /locus_tag="M5T26_RS27260"
                     /old_locus_tag="M5T26_27260"
     CDS             complement(3676..3924)
                     /locus_tag="M5T26_RS27260"
                     /old_locus_tag="M5T26_27260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259429149.1"
                     /translation="MVRSITEDRKTIKKEKRGLFEEKLAIRFFFGEIMKKKKLQKKKRR
                     NKNKHSKFFKMLMKSVNEAVKIHKGELEPARVTILKM"
                     /name="hypothetical protein"
     Typing          2485..3641
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.705"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgataatac aagaagaaca taacatcgat ttttttaaaa atcaaatgcc taacaaacct
       61 tattgcacaa ataacctcga tatgggatta agcataagga acaaggcaaa agcccttgaa
      121 atgctgtatt tacaggcaaa tcagccagcc attcaaactt gcttactctt tgatttagat
      181 aagaaaaatt cattctatac atttgagaag gtgggcttac ccatacctca ctttattaca
      241 aaaacaccaa agacaggtcg ttgtcactac ggctatatgc tgaaagccgg ggtttgtaag
      301 acacaacaag caaggttgaa gccgttgaag tatgccgcag ccgttgaaat ggctatggcg
      361 aaaaaactca aagcagatct ggggtttgct ggattgatta caaaaaaccc actcaatgat
      421 cactggtcgc cgttctggtc aggtgctgac ctttatgacc tggattattt agctgatttt
      481 gttgacttag aaaagcctca aatacaaaag aaaaaaagcg aggcttatgg tttaggtcgc
      541 aatgttaatt tgtttgaaga tctacgccaa tatgcttaca gaacggtttt aaatttcaaa
      601 aaaatatcta ctttcgagaa attcgaaaac gaactattat taaaagcaca gggtttaaat
      661 actttttgca acccacaaaa cccgttacaa tacaaagagt taaaagcaac agcaagaagc
      721 atagcccgtt ggacttggaa aaattttgat agccatacat tttcattaat acaatcaaat
      781 agaggtttaa aaaataaagg caaaactaaa aaaaccaatg ttaaaaatga aatgttagac
      841 atattaatga gcaagggggt tttatgatca ttaatggtaa aaaaataaaa gcaaaagaaa
      901 taatggaaat gacgggtcgt agtgaaagaa ctgtcagaaa atatttttct caatctcgtg
      961 acgattacga aaaaacagca atggatcgtc gtcgtcaggc ttacgaacta cgaagccagg
     1021 ggctgaaatg gcaacaggta gccgacaaaa tgggctgttc gtatcacgga gccgttgccc
     1081 tgtatcgtcg ttatgttgct ttggatatgc ctcaaaacag cctgtaacga cttatgagag
     1141 gtttttcccc ctaacctaac caaaccctcc acccctgggg ggattttttt ccctttcccc
     1201 gtatccgggc cctgtatctc gaactgagag gcactcaggt acccaacaat caacaaaccc
     1261 acccataagc cctgagagcc tctctgagcc tttttaagac ctccaggcaa gcctggcaac
     1321 ccttcacagt aaaaaaccag ccaaaaagcg gctggttctt cactgttcac ggtttacgat
     1381 tttcattttt ccactcttcc aagatattca ttccgatccg tgtaaaatct cttttctctt
     1441 cttcgtctaa ctctttgatt ttacttagta aacctaataa aagagcttta tttacttctt
     1501 ccggtttact atttaaaacc tttgcaactt ctgcacccaa tataattttt tgccttgttt
     1561 cttctcttct gtcctgtctt gccttttttg ttcttaatgc ttgtaattta ttttgtagat
     1621 ctgcgatctg ttgtgctgtt gatttactca ttattaacct cctcctcaac cccccaactc
     1681 attcccattt cgtttatttc aaaatgaatt ttatataatt ccaaaattcc catttcagaa
     1741 accattgttt ttaaccctga cataatatct tcaaccataa tcctttcaat atcgactttt
     1801 ggctttttca ttttagttaa tgctcctaaa ttatcggcta tgttttccct tgtagcctca
     1861 agagcttttt ctatatcaaa attattacaa tgataatgac gcaaaaataa atctattaaa
     1921 gccggaatgc ttgaaattgg gaaaccttcc tcaatgtgaa ctgtaaatat attttccata
     1981 caacctccat tattttattt tgttaattct tcacttgacc aacgaccata agatttatcc
     2041 ccacatacac aacaggtatc aaacagcctt aaaacctctt cgtaatcgtc ataaaactca
     2101 ccattcatat aagaagccaa ttcagccggt gaatactccc aatcgtcttt acttaaagat
     2161 ttttccgtta cataaccctc ttctctttcg tgttctgttt ctttcctaca cttacaacac
     2221 tttttatata tatttttata ttccatacaa cctcctaatt atttatttta ttatttatta
     2281 cctccattat ttactttagt caaatatatt ttaaaataaa acaaataata tttaaattgg
     2341 gcgaagccgt tttattagtt cattatcttt ttgagccgtg gcgaaaatta gataatgccc
     2401 acttatacac tcgttccgag tgtgtgggcc ctgcggggct aaaacccaaa attcaaaaga
     2461 taaaaaaagt aagggggtag cctatggcta tatatcattt aagttttaaa ttgtgcaaaa
     2521 ggtcagaggg taaatcttcc gtctatttgg ctgcatatca gaatagaaat aaatttaccg
     2581 ataatagaac gggtgccgta tggaattaca gcaaaaaaga aggctttgac ggttcgttaa
     2641 tcctggcacc accacaagca ccaggatggc ttgtgaagtc gtctgaggcc ctatggaatg
     2701 aggttgagag gacagaacga cagaagaacg gacagactgc caggtacttc gatgtcgcct
     2761 tgccggtgga gctggacgac aaacaaaaaa aagacctttt aataaaatac tgtcaggata
     2821 attttgtaga tgagggaatg attgccgata ttgcattcca cgatctaaat agtaaaaatc
     2881 cccatgccca tgtaatgtta acaatgaaaa atgttgggcc agagggattt ttaacaaaaa
     2941 acagagaatg gaacgataag aaatgtttag aagcctggag gcaaaattgg gaagttatta
     3001 caaatgaggc attaaaaaaa tatggtcata aatcccgtat tgattcccgt tcattggaag
     3061 aacaaaaaga agaggctgac agaaaagcaa aagttgcaaa aactccacag gcaaaagctg
     3121 cctggattgc taaatctatc cgtctggaca gaacgccaat gacccgcatt cacaggcata
     3181 attggaaaac tgggcagaaa ttaagaaaat tggagcaaga agaaaggaag attttatata
     3241 aaaaggcttc gttaacatat caccaaataa ctaaaccaat tgagcctgag caatctatga
     3301 aaatagaaaa atcacaaata gctcaaaaaa taaaatctat attcgaaact gccaaaaata
     3361 aattaaattc gaaaatcgct gactttttca agcctaaacc caaaaaagaa actcctgaaa
     3421 aatttgtcag tagttatgaa attgacgagt taggaaatca catattgaaa aaagaggctg
     3481 aaaattggga tcagaaaaac aatgataaaa ttaaaaagca ggttcgtgag aaaaaagaaa
     3541 aagaagaatc cgaaaggttg aggcttaaag aagaggatga gaaattaaaa aatagctcca
     3601 agccaattca gcaaaataac aatcatagga aattaaagcg atagtatata ttgctatata
     3661 ctacaatata ctatactaca ttttcaaaat tgttactctg gctggctcta actcaccttt
     3721 gtgaattttc acagcttcat taactgactt cataagcatt ttaaaaaatt tactatgctt
     3781 atttttattt cttctttttt tcttctgcaa tttctttttt ttcataattt caccaaaaaa
     3841 aaatcgaatc gccaattttt cttcgaacaa tcctcttttt tcttttttta ttgtttttct
     3901 atcttcggta atgcttcgca ccatagatct gatagtaatg ctttgctgcc gtatgtaaag
     3961 aactgcccca acttcgttgg gctgttatct gatatggtta agacgcacac ttgacaaatt
     4021 tttgagatcg gttagaactg gtacaggatt gaaaaatcta taaaaataaa aaccccaccc
     4081 ctctgtgatt gttttggcga cagaacagag gagcagggcc agataaaaaa ggactgaata
     4141 aaa
//
