LOCUS       NZ_CP103486             1505 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli strain 4621 plasmid pMB9635_7, complete sequence.
ACCESSION   NZ_CP103486
VERSION     NZ_CP103486.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174065
            Assembly: GCF_024917715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 345x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:32:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,329
            CDSs (total)                      :: 5,210
            Genes (coding)                    :: 4,989
            CDSs (with protein)               :: 4,989
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 89 of 221
            Pseudo Genes (incomplete)         :: 150 of 221
            Pseudo Genes (internal stop)      :: 43 of 221
            Pseudo Genes (multiple problems)  :: 54 of 221
            Pseudo Genes (short protein)      :: 4 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103486.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4621"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB9635_7"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-12-22"
                     /collected_by="MDACC"
                     /genotype="ST68"
     gene            1..441
                     /locus_tag="M5T46_RS26540"
                     /old_locus_tag="M5T46_26540"
     CDS             1..441
                     /locus_tag="M5T46_RS26540"
                     /old_locus_tag="M5T46_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          complement(726..982)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="98.0"
                     /evalue="3.06e-107"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagttggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccggattta aagagccagc ggcaggcgga caatcagggc tacgtgttaa
      481 cgttttgacc atgattgtct atcctgcatt gctcttttgc cgcatgtcag ttcgatgcga
      541 tgcaggatag ggcaaacgcc gcaaaatgac gtcattgacg tcattcgcaa cctttgcccg
      601 ataatctcca aaacgtctga gctgccacgg ttccggcagc gccttgagcg cagccggaac
      661 cgtctgggga gaaccccaga cccccatcgc tgtaacatcg acggtctacg accaccaacc
      721 agctgaatag ctgccagtgc cgtgcaaatg ggctttgcag ggggctgacg cggttcctgg
      781 cgacctgaaa gcccctggtt aggggtatcg gcgcgcctga cctcctgaaa ccctctgtaa
      841 gcgtttttcg cgccttccat gccctgacgg gcatttaaaa cccgtttcgt gcgcctgaga
      901 gcttctgaga gcgtttgaag agctatctgg cgactcatcc ccgaaagaac aacagcggaa
      961 tcaccgataa catgcgagag gtggcaccgc tcgccgcagc cccagcgacc gaacgttagt
     1021 gagcgagtgg gcgaggaagc ggaagagaac cggaaaccac attgagcacg tacgcactga
     1081 tgcggggtgt cggggtgaag ccctgaccag atggcaaacg gaatatcgtc gcgggtgacg
     1141 gtattacatt tgcacatcct gtcccgattt ctgcggagtt taacggtgaa cggacggcaa
     1201 aaaacgtgca ggagctgttg aacgtgaaaa cctcgaaatc tggcaaaaat cacggctgac
     1261 gaggcttgca gaggattaag ccagtataca ctccgctatc gttacgtgac tgggtcaggg
     1321 cttcgccccg acaccccaaa gagtagctag gataaatatc taatggaatc aatgcgttag
     1381 acgcctaata taaagataca gaaaggagaa taaaaacatt aacaaaatct aagggttaca
     1441 gttgacatag ttatcaataa tgataacact gttatcatta ttgataactc aaatagggtt
     1501 caaag
//
