LOCUS       NZ_CP103482            36884 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli strain 4621 plasmid pMB9635_3, complete sequence.
ACCESSION   NZ_CP103482
VERSION     NZ_CP103482.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174065
            Assembly: GCF_024917715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36884)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 36884)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 345x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:32:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,329
            CDSs (total)                      :: 5,210
            Genes (coding)                    :: 4,989
            CDSs (with protein)               :: 4,989
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 89 of 221
            Pseudo Genes (incomplete)         :: 150 of 221
            Pseudo Genes (internal stop)      :: 43 of 221
            Pseudo Genes (multiple problems)  :: 54 of 221
            Pseudo Genes (short protein)      :: 4 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103482.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..36884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4621"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB9635_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-12-22"
                     /collected_by="MDACC"
                     /genotype="ST68"
     gene            1..678
                     /locus_tag="M5T46_RS26250"
                     /old_locus_tag="M5T46_26250"
     CDS             1..678
                     /locus_tag="M5T46_RS26250"
                     /old_locus_tag="M5T46_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962071.1"
                     /translation="MINDKSFNIENIISDIFKETRLKISKDDPVLSIVLMHEKILEHAL
                     TQLKNSNQIATERLSHDISSIRDAINALPEAIDEKTSELQHAAVALHDEFQESKGEIKG
                     SLEEARINATEKLAESAKELQLNITKVAEKTTETIDTVNKIISNIETELYDINKKALHD
                     YVKEIKLVDEKRKSITTNLESAINDAFKKSVKSFKFYCSAALIISTLLQFTMWGFFLYK
                     LLT"
                     /name="hypothetical protein"
     gene            complement(1438..1740)
                     /locus_tag="M5T46_RS26255"
                     /old_locus_tag="M5T46_26255"
     CDS             complement(1438..1740)
                     /locus_tag="M5T46_RS26255"
                     /old_locus_tag="M5T46_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="WP_072120077.1"
                     /translation="MDTFDSEKFLSELDILSKEIMPGCGLVFELYQRRLSAAIDKVVAR
                     LPEEQHAQAFELARKEFDYLSAKEIAEEIRNGYANGYCCHGFDRDCCPLGCGDLP"
                     /name="CcgAII protein"
     gene            complement(1817..2050)
                     /locus_tag="M5T46_RS26260"
                     /old_locus_tag="M5T46_26260"
     CDS             complement(1817..2050)
                     /locus_tag="M5T46_RS26260"
                     /old_locus_tag="M5T46_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962084.1"
                     /translation="MTSEVIKQIQKIQDRGIIIYSKYRAAEFDQDDVYRESYFLVVAFN
                     ELIAENIIHDENLVDQTACILHELRRIAIEGK"
                     /name="hypothetical protein"
     gene            complement(2477..2767)
                     /locus_tag="M5T46_RS26265"
                     /old_locus_tag="M5T46_26265"
     CDS             complement(2477..2767)
                     /locus_tag="M5T46_RS26265"
                     /old_locus_tag="M5T46_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231239181.1"
                     /translation="MMINEAKKMAVEMMFWTACPVDDDNCFDAIMNASKIDRAFEDKYG
                     VYLHPIYKDLLSTVQIKSALKKRAIKIISFKCDINEDSSRKILMQTILRAV"
                     /name="hypothetical protein"
     gene            complement(3069..3419)
                     /locus_tag="M5T46_RS26270"
                     /old_locus_tag="M5T46_26270"
     CDS             complement(3069..3419)
                     /locus_tag="M5T46_RS26270"
                     /old_locus_tag="M5T46_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962082.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREGLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGNGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            complement(3511..3810)
                     /locus_tag="M5T46_RS26275"
                     /old_locus_tag="M5T46_26275"
     CDS             complement(3511..3810)
                     /locus_tag="M5T46_RS26275"
                     /old_locus_tag="M5T46_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077814927.1"
                     /translation="MKCSPVFTSTTNYVFTLERVTLCTIALIHKDTGQQYVVIFTDNNK
                     IRDYKTGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLR"
                     /name="hypothetical protein"
     gene            complement(4214..4471)
                     /locus_tag="M5T46_RS26280"
                     /old_locus_tag="M5T46_26280"
     CDS             complement(4214..4471)
                     /locus_tag="M5T46_RS26280"
                     /old_locus_tag="M5T46_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_052962080.1"
                     /translation="MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQ
                     LNLSKGEFEYLLLLAGEHPEYVLCKRGNMTHNEINEQLRP"
                     /name="transcriptional regulator"
     gene            complement(4468..4722)
                     /locus_tag="M5T46_RS26285"
                     /old_locus_tag="M5T46_26285"
     CDS             complement(4468..4722)
                     /locus_tag="M5T46_RS26285"
                     /old_locus_tag="M5T46_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962079.1"
                     /translation="MRKNKGRLTYYLEMIDEKYHFVKKISSYSKVYTEGKTKTTKRTLS
                     ELVFNENEVGAIDFTKNGLKPVDKSILLTMMKEYKENET"
                     /name="hypothetical protein"
     gene            complement(4719..5261)
                     /locus_tag="M5T46_RS26290"
                     /old_locus_tag="M5T46_26290"
     CDS             complement(4719..5261)
                     /locus_tag="M5T46_RS26290"
                     /old_locus_tag="M5T46_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962078.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCPSRIKLTSPQFKNY
                     SVHIRMEKERFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILINASQEVLQA
                     IEYEKSQEVKKDEILILKGMLSQLVQLESWYGALTGFKVENGLNGKVIKQGERYDLQIR
                     GLNIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(5365..5880)
                     /locus_tag="M5T46_RS26295"
                     /old_locus_tag="M5T46_26295"
     CDS             complement(5365..5880)
                     /locus_tag="M5T46_RS26295"
                     /old_locus_tag="M5T46_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_048267080.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDFLMANIDKINQFQSTDEHSRYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GDTITHKETLKEIGCKWAAKKKQWFYRPEEHKRYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVENRA"
                     /name="J domain-containing protein"
     gene            6463..7128
                     /locus_tag="M5T46_RS26300"
                     /old_locus_tag="M5T46_26300"
     CDS             6463..7128
                     /locus_tag="M5T46_RS26300"
                     /old_locus_tag="M5T46_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353182.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_052962077.1"
                     /translation="MAIYAISISKGGVGKTTSAVHIIDEIKPDVVFDLDLHHSLSIINR
                     LRPAEKKWSIVTVSDKAELLTYLKKINDEGKTAVIDCGGFDADINRIAIAVADVIIVPA
                     NDDVTEQIGLATFDAVLAEISRQTGRNIQAHVLMCKTQPNQKNFPDMDDTMKKVKHMAL
                     LNARLSYRKGRYGFTDSLRKGMGITQIKHGRSSQAGREVIELVKELRALSSDNTGFPA"
                     /name="ParA family protein"
     gene            7201..7440
                     /locus_tag="M5T46_RS26305"
                     /old_locus_tag="M5T46_26305"
     CDS             7201..7440
                     /locus_tag="M5T46_RS26305"
                     /old_locus_tag="M5T46_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962076.1"
                     /translation="MKNKQSIFDAALNIGNEAPLQVKNETPKQRTVRTKTLKAIPTRYF
                     DAHLKLRETNKTNLDFSNYIMEALREKLEKDGGL"
                     /name="hypothetical protein"
     gene            7595..7849
                     /locus_tag="M5T46_RS26310"
                     /old_locus_tag="M5T46_26310"
     CDS             7595..7849
                     /locus_tag="M5T46_RS26310"
                     /old_locus_tag="M5T46_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
                     /protein_id="WP_052962075.1"
                     /translation="MKITTLSSRELNQDVTRAKRATSDGPVFITDRGKTAHVLLSIEEY
                     RKLTQQRRTLIDILAMPDADDVEFEPQRINVGLKPAEFL"
                     /name="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
     gene            7849..8265
                     /locus_tag="M5T46_RS26315"
                     /old_locus_tag="M5T46_26315"
     CDS             7849..8265
                     /locus_tag="M5T46_RS26315"
                     /old_locus_tag="M5T46_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012987825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family toxin"
                     /protein_id="WP_052962074.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFVSVITIME
                     LEQGILQVERRDIRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCARLHVPDRRSERD
                     ALIAATALVHGMTVVTRNVGDFELSGVSIINPWD"
                     /name="type II toxin-antitoxin system VapC family toxin"
     gene            complement(8390..8551)
                     /locus_tag="M5T46_RS26320"
                     /old_locus_tag="M5T46_26320"
     CDS             complement(8390..8551)
                     /locus_tag="M5T46_RS26320"
                     /old_locus_tag="M5T46_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001128311.1"
                     /translation="MPLIEYINKFYRGNQASFARLTGVQPAQVTQWINKGFIVVNHTLY
                     SPRRKLGI"
                     /name="hypothetical protein"
     gene            complement(8620..8807)
                     /locus_tag="M5T46_RS26660"
                     /pseudo=""
     CDS             complement(8620..8807)
                     /locus_tag="M5T46_RS26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(9507..10529)
                     /gene="repA"
                     /locus_tag="M5T46_RS26325"
                     /old_locus_tag="M5T46_26325"
     CDS             complement(9507..10529)
                     /gene="repA"
                     /locus_tag="M5T46_RS26325"
                     /old_locus_tag="M5T46_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="WP_016234216.1"
                     /translation="MASLNRPSLHWSELSPEEQILFWEDYESGRATSFLVEPEKRSTKR
                     RRGEHSTKPKCENPAWFRPDSYKALGGQLGHAYNRLVKKDPVTGQYTLRMHMSLHPFYV
                     LKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDSKGNVI
                     PETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKMLGVDLVK
                     LQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKFRREKAAKRK
                     RANRLAKLPYDEQRNEIARFILKRMSPDEAYWCTKERLEQLVARDLRQLELALTASPPH
                     "
                     /name="repA"
     gene            11420..11602
                     /locus_tag="M5T46_RS26330"
                     /old_locus_tag="M5T46_26330"
     CDS             11420..11602
                     /locus_tag="M5T46_RS26330"
                     /old_locus_tag="M5T46_26330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962073.1"
                     /translation="MRDDNYLDGLIAGGTVAISFAGLILLIVSNLKNEAETEKTAFYEW
                     ADSHCEKIEKNFKCY"
                     /name="hypothetical protein"
     gene            11674..11958
                     /locus_tag="M5T46_RS26335"
                     /old_locus_tag="M5T46_26335"
     CDS             11674..11958
                     /locus_tag="M5T46_RS26335"
                     /old_locus_tag="M5T46_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001330654.1"
                     /translation="MSQAKANLKKLRHHCYIPPKQKISRLYYVEKALSSPWIYRERPAY
                     RAELLLTHDTLLVIYGLAKSGLIDRSEESLNREIKGVRKALAAGRFRWS"
                     /name="hypothetical protein"
     gene            12546..13127
                     /locus_tag="M5T46_RS26340"
                     /old_locus_tag="M5T46_26340"
     CDS             12546..13127
                     /locus_tag="M5T46_RS26340"
                     /old_locus_tag="M5T46_26340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_077817892.1"
                     /translation="MTLISQCAPGVSPETMRAIIMTESGGNPYAIANVTDGGSKYFTTE
                     EEAVNHAKKLTTDKKNFSAGLGQINSRNFQTLNLTHESVFSPCANIRAAAAVLKTCWDK
                     YETKGGDQQKILRDAMSCYYSGNNKRGYVKESNGKSYIDIIEEKAGIHTHYDIPAIKSY
                     SETSSHLMVSSDNHDKWDVFGDFGIQDFKE"
                     /name="lytic transglycosylase domain-containing protein"
     gene            13209..13514
                     /locus_tag="M5T46_RS26345"
                     /old_locus_tag="M5T46_26345"
     CDS             13209..13514
                     /locus_tag="M5T46_RS26345"
                     /old_locus_tag="M5T46_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231239180.1"
                     /translation="MNKSIALYRTFFLFLLCSFIATNPALAAGVGGLDKGTNFIGELRL
                     WAYGFVGGVALLHILWKVFQAYTEMDSWAGVLKSLMYAAIAGGCVVAAEACYQIFK"
                     /name="hypothetical protein"
     gene            13569..13892
                     /locus_tag="M5T46_RS26350"
                     /old_locus_tag="M5T46_26350"
     CDS             13569..13892
                     /locus_tag="M5T46_RS26350"
                     /old_locus_tag="M5T46_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_000356611.1"
                     /translation="MDKEEIKAAEELKAIFLTYNGLNRPAMIKGMPIIPFILCLLALMV
                     SFFVGILTIDFYGLIIPSIIIGVMIAIKQMCADDPNAMSARALKFKGLCLKGFRSNNVL
                     KVE"
                     /name="VirB3 family type IV secretion system protein"
     gene            13894..16323
                     /locus_tag="M5T46_RS26355"
                     /old_locus_tag="M5T46_26355"
     CDS             13894..16323
                     /locus_tag="M5T46_RS26355"
                     /old_locus_tag="M5T46_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="WP_000023358.1"
                     /translation="MSIKKITNNFIPPYHSHIHENIIWLEDKKLLLTFLIEGIPYESLT
                     DDMILNNFNGQKETLLGLCKSERVFLWKHLVKRESPMDARYKFPGNPFLQRLGDFYCDT
                     FNGERSFRTDYFLTIGIPYDDIDAGEKKAKDIIRQIETGFKDYNIYTLGISDGGISDIC
                     SNYLNYLLNHDFEKIPLSATKISESIKNSKFYFSFDTLEIKNRTGHDNKFCSNYIVKDV
                     PRGSQIGQWNFLLSLPFEFILTQSFIPDSLSKSSKKIEQQLNKLTSVKDAGKTQHTELH
                     MAQEAIINQETVFGSWHSVLSVFGNTPDEASDNGSEVAAEFVTSGRGFRFVRAASEAPY
                     AWFSHLPASKLRPLATQRTLTNVCCLMPLHNHSYGKKSGNPIGDGSAIMPLKTVTDGLY
                     WFNTHYSAPGRNVTGQMIAGHGMLLGATGAGKTTFEMMAAAFIQRFNPDMFVIDFNRST
                     ELAIRMFGGQYFSLSEGVYSGLNPFQIGDNDDPELMAFLKEWVKRCAVNNDGSDCSDSE
                     AEEIDRAVEMVMKLERRQRRFARLQSRIQSPELNVRLSKWWDNGALAWATDSEENKFDP
                     NQYSKIGFDTTVILETVNQRDHPACEVILSVLFFYKKRMQKGGKLMLSIVEEFWKPCNY
                     PMTQALIKASLKAGRMKGEMMWLTSQSPEDAINCQIFAALVQQTPTKILLPNPDAKYSG
                     YKEIGLTEKEFRQLKKLKLESRTMLIKQSGSSVFAKMDLVGFDDFLPVISCSMKGIKLC
                     EKIREEIGSDDPELWYPPFIEGIYQIDDVIREANSDIPEVWLPFMMKRLKDKLPEK"
                     /name="VirB4 family type IV secretion system protein"
     gene            16546..17214
                     /locus_tag="M5T46_RS26360"
                     /old_locus_tag="M5T46_26360"
     CDS             16546..17214
                     /locus_tag="M5T46_RS26360"
                     /old_locus_tag="M5T46_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000753300.1"
                     /translation="MKKTLTALFLGATLTGYSSVASSVGGVIVKDPGSIAKTVEVIKKA
                     ADQIAELKKQVDLATSQLEAYKQEVLDTKRRLEGITDYSAIFGSAESYMKDFWEDMKKE
                     LTDADIRSMATKYGFDTKEYDRIKRQYESKFEEITKYEAMSKDLEKSAEKIKATQKAFS
                     KADTPQKREELQNNILLETAAMQLKIAQNEAEINRMARERKLREEAALIKYTEDNFSFN
                     "
                     /name="type IV secretion system protein"
     gene            17307..17654
                     /locus_tag="M5T46_RS26365"
                     /old_locus_tag="M5T46_26365"
     CDS             17307..17654
                     /locus_tag="M5T46_RS26365"
                     /old_locus_tag="M5T46_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053295590.1"
                     /translation="MFKKLLGGFILAFAFLLSGCGGDNGELAGTYIEKNPDSGDAPQKW
                     IIKYDKKDNYYTQIWIEKDKKGNEHNGTTNSFLIRDGDWLAGKEKHFIRVIDKNTLQLR
                     DDENALYIRTN"
                     /name="hypothetical protein"
     gene            17720..18766
                     /locus_tag="M5T46_RS26370"
                     /old_locus_tag="M5T46_26370"
     CDS             17720..18766
                     /locus_tag="M5T46_RS26370"
                     /old_locus_tag="M5T46_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000157854.1"
                     /translation="MASNDSAFAKANFEIVQPMFNELSSIIQNSVTTNFQTIMQLVTPL
                     FGAAVILYGVFFAWEIMYTRKDDVMMESVKRMGVLTLVFAMSTAGGMYLSYIVPFVQGS
                     GQEVASRLIGQDGASTGAVIDGLLNRLIDLGHEQFKVFQKADGWFDSMGAWIVFILKAI
                     LLALAGGGFVIYCAAYLLMAEMMVGILLSIGGVFILFSAWPTTRNMFTAWVGSCFNYIF
                     LNIAYACLFKVLIQYINNYINTNSDDITKNIWSVLCVAVVFLIGKFLLAQISTLISTLT
                     GGVGINGLTSATGDVIKSLRGAGNSTRNQLGLGFNKTAQDARARNSEYAAAAKDYLSSR
                     FGGNIKAG"
                     /name="type IV secretion system protein"
     gene            18967..19713
                     /locus_tag="M5T46_RS26375"
                     /old_locus_tag="M5T46_26375"
     CDS             18967..19713
                     /locus_tag="M5T46_RS26375"
                     /old_locus_tag="M5T46_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000523226.1"
                     /translation="MGLKEYFKRSKAEARKTPAQKETDKKIEKNVLDAAKEFARDRDNE
                     LKRSLRITRFALFFSSASAAAMAVALAVMAPLKTTEPFLLRVDKTTGYTDVITPFSQDS
                     GTYDEAVSRYFLTRYLENREGYEWYTIQNMLNTVELMSSSQVFAEYNNMIHGPLSPLYK
                     LKKSYKILVRVQSITFLDENTVQGRFVKAITDPDGKPAPGYTPTQWIATIKFVWLKDKI
                     ATEAERQINPFGLDVLSYRVDPEVVK"
                     /name="type IV secretion system protein"
     gene            19710..20507
                     /locus_tag="M5T46_RS26380"
                     /old_locus_tag="M5T46_26380"
     CDS             19710..20507
                     /locus_tag="M5T46_RS26380"
                     /old_locus_tag="M5T46_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="WP_000980278.1"
                     /translation="MMMRKLLLSLVISGITMTCHAAKTPASAGFDRRVQQIVYNPNDVT
                     IVKTKAGEVTLIELEEGEIIKSEDTGLAIGDPEAWDPAVRGNTIFLRPKAAEPDTNIAI
                     VTNKRIYSIQLVSTDQNPTYLLRYIYPKPPEPVKPNVMTGRSRVVAPCTEGNIVNGRYE
                     VRGSRKIKPEAIWDNGRLTCFRWTGAADLPVVFRILPDGKEQLVNYHMDKNIMVVHEIS
                     PAFVLRLGGEVMEVRTNRHIPRAWNASGTLTGETRVEINNDEK"
                     /name="TrbG/VirB9 family P-type conjugative transfer
                     protein"
     gene            20497..21720
                     /locus_tag="M5T46_RS26385"
                     /old_locus_tag="M5T46_26385"
     CDS             20497..21720
                     /locus_tag="M5T46_RS26385"
                     /old_locus_tag="M5T46_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="WP_000794678.1"
                     /translation="MKNNNDIVIEKLDKIDTQEADTDAQNRNFIHSGKKQGNKKAKAVL
                     ILAGFAFVTVVILTAVLSKVFSSSGEEETTETATTKAQAEVYAQNTQAKNLDDLKESVA
                     RSIEEVKNNVNSEEEQAREEAPPENKENKGVTLPAENEADTGYTQSNGDAPPTPEQRRL
                     MGSVMVDDSRITQSQPGSSGQKTAGATPASDNTNVSGDGDFLQGADFADGSVSRIKNRR
                     FLLSAGTTFPCVLKTMLVTTYPGVTLCQLTKDVISDDGKTVLIRAGAQLQGEQTKAITQ
                     GMARVFVNWTMVKDRNVRVRINALGADSLGASGLPAWVDTHFWERFGGALMLSLIEDTL
                     AAGTSRLSRENNSDFTMNNTTGTSSELAKTTLDNSINIPPTGYVNQGEMLTVIVPRNID
                     FSSVYKVR"
                     /name="TrbI/VirB10 family protein"
     gene            21720..22733
                     /gene="virB11"
                     /locus_tag="M5T46_RS26390"
                     /old_locus_tag="M5T46_26390"
     CDS             21720..22733
                     /gene="virB11"
                     /locus_tag="M5T46_RS26390"
                     /old_locus_tag="M5T46_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234221.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000447880.1"
                     /translation="METINMFLESTGVRPFLQAEGLTEIIVNRPFEIITEGVNGWEYHE
                     APGASYSDLLDLSIAINKYNNSAEPLDALHPIKSLVMPGGERMQIIMPPACEERCISFT
                     IRKPSLRRFTLEDYVSSNRFSNVRIVNQANLSTLSASQQKLLDLLDNTRCAPRTWISFL
                     EAAVAARMNILVVGGTGSGKTTIAKTIADLFPRDRRIITIEDVHEMTLPNHRNHVHLFY
                     KQGGVQPRYLIEAAMRMKPDHIFLAELRGDEAWSYLEALNTGHEGSVTTIHANSALDAI
                     SRLASIVKQSTVGLTLDHSFILKTIKTSIDIVVFFRHTHMTEIYYNPMEKNQLLAA"
                     /name="virB11"
     gene            23053..25074
                     /locus_tag="M5T46_RS26395"
                     /old_locus_tag="M5T46_26395"
     CDS             23053..25074
                     /locus_tag="M5T46_RS26395"
                     /old_locus_tag="M5T46_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727494.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_053295591.1"
                     /translation="MKKKKLVLPVLIVWLMALTASGWLGSFILVKIASLPTDNLQWSTY
                     LSTLAYYGWQPSLKKYLFAGLAGTLLPFLIALILTVVMFVAVREKKTVHGNARLANDRD
                     LADSGFFPDEKELKNKKYPSLLIGKMPEHKGRFKSRYLQYSGQQFLMLYAPTRSGKGVG
                     IVIPNGIWYTDSMVFLDIKLENFLETAGYRKNVLNQEVYLFSPDGYAREISGETVLTTH
                     RYNPLYYIRRDPVYRFGDLEKIAVILFPMTGGENDTWVESSKNVFISLVLWLLDTELEG
                     KFKVNMNSVMKLSIPADGSSLAKWMLNEIRSRGNEVLTERWKAYHALPEEEKQGKTPST
                     YPLSDDTIRLFRQFAAKEPEQQESIMLDFNRTMSMFANPVVAAATEDNDFDMRDVRRKK
                     MTIYYGISPGALKQYQRLTNLFFSQLLNENVRTLPEHDATLKYQCTLMLDEFTSMGRLD
                     VVQVSLAFTAGYNLRFVFILQNREQLFDEKHGYGKMGGNTILKNCAVEIFYPPKKVDES
                     VKDVSETIGYYDFVRTQISRTTGKNPSTTRNKIIEKRAVMLPQEITDLRDQKFKVKGKK
                     TSFSLPQIVMSEFCRPFIAHKIVSFDQNNESFFVNARKYSRKNIIDTPVLDIPQDEVVR
                     QLMLQREREEAWKATQPAFDKNDFDDDKEYFPGDGGGD"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            25322..25651
                     /locus_tag="M5T46_RS26400"
                     /old_locus_tag="M5T46_26400"
     CDS             25322..25651
                     /locus_tag="M5T46_RS26400"
                     /old_locus_tag="M5T46_26400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231239185.1"
                     /translation="MKPRLLISVTLLSALTFILPASAKIKGLSAAGDPCEVLVCMAEKV
                     QGAPQPGCIPVNQRFFNIRVYTPFYNPGATYGARQNFLYTCSGSAVNQATLNMIMQRYG
                     SLFSG"
                     /name="hypothetical protein"
     gene            25726..26064
                     /locus_tag="M5T46_RS26405"
                     /old_locus_tag="M5T46_26405"
     CDS             25726..26064
                     /locus_tag="M5T46_RS26405"
                     /old_locus_tag="M5T46_26405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000008122.1"
                     /translation="MSFIKINRQREFMERSNSPRLVLRKEKRRNGIVNCYLSKTVRGDD
                     TFITIELNPEHNKIRLKTTNSPEGHKMSRGCFSLTKVFEYIFHENLHEDRIIIELEKKE
                     NGWWYGNY"
                     /name="hypothetical protein"
     gene            26130..26717
                     /locus_tag="M5T46_RS26410"
                     /old_locus_tag="M5T46_26410"
     CDS             26130..26717
                     /locus_tag="M5T46_RS26410"
                     /old_locus_tag="M5T46_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="WP_155018583.1"
                     /translation="MYLHIVPKLFHRMANKCTLKSISIPELDLIIDGESLSVGRPWPNK
                     CVWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHQVNTYIEDDDFDMVSQ
                     EILLNGSFDKWSNRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLLSRE
                     ESLLLHTIQSERLNTDCSLFKRQPSIESALVI"
                     /name="DUF6012 family protein"
     gene            26742..27317
                     /locus_tag="M5T46_RS26415"
                     /old_locus_tag="M5T46_26415"
     CDS             26742..27317
                     /locus_tag="M5T46_RS26415"
                     /old_locus_tag="M5T46_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001199074.1"
                     /translation="MQYALVDALERKFLLDALEFGVLKDWKENPVKELPDIDESVHPFH
                     VCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLILCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKDQDIATNILNREGIFAFNGCERRFRQ
                     PVSEDNWQQAVSEERAIRCANRLIQCKG"
                     /name="hypothetical protein"
     gene            27322..29475
                     /locus_tag="M5T46_RS26420"
                     /old_locus_tag="M5T46_26420"
     CDS             27322..29475
                     /locus_tag="M5T46_RS26420"
                     /old_locus_tag="M5T46_26420"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235750.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSSRKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFYGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            29472..29678
                     /gene="hha"
                     /locus_tag="M5T46_RS26425"
                     /old_locus_tag="M5T46_26425"
     CDS             29472..29678
                     /gene="hha"
                     /locus_tag="M5T46_RS26425"
                     /old_locus_tag="M5T46_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            29696..30130
                     /locus_tag="M5T46_RS26430"
                     /old_locus_tag="M5T46_26430"
     CDS             29696..30130
                     /locus_tag="M5T46_RS26430"
                     /old_locus_tag="M5T46_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293126.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293139.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREVDFEQLLEMQEKLNVV
                     VEERREDAEREAAERAERERKRQELLQLIAGEGFSPEELLGITEDAPKTRKKTLPKAPP
                     KYQFEENGETKYWSGRGRAPKPIAEALAGGRSLDEFLIEK"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            30529..30849
                     /locus_tag="M5T46_RS26435"
                     /old_locus_tag="M5T46_26435"
     CDS             30529..30849
                     /locus_tag="M5T46_RS26435"
                     /old_locus_tag="M5T46_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053295602.1"
                     /translation="MKFFILKKNKDSTITKNDTVFPVNNFFVVLTLVSSLVNSAGIFFP
                     MISHSGNIIEMCFFVMFSVLLAAFSFVSTMGLANAAAPIKNVFPLLLLVIVLITNYNIY
                     FR"
                     /name="hypothetical protein"
     gene            complement(31170..31265)
                     /gene="dqlB"
                     /locus_tag="M5T46_RS26665"
     CDS             complement(31170..31265)
                     /gene="dqlB"
                     /locus_tag="M5T46_RS26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609149.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            complement(31632..34919)
                     /locus_tag="M5T46_RS26440"
                     /old_locus_tag="M5T46_26440"
     CDS             complement(31632..34919)
                     /locus_tag="M5T46_RS26440"
                     /old_locus_tag="M5T46_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="WP_155018582.1"
                     /translation="MLVRISGGNKGVCEYLEKGHKQGREYTRDEIDHRLVLDGDLALTE
                     SVIDAISDNGQERYMHITLSFFEDDVSESKLHDVVQEYKSLMMNAYHEDEYNFYAEAHL
                     PKIKQMPDHKTGEMVERKPHIHIVIPEVNLVTGNKLNPGGVINHYLKHLDAIQEHINNK
                     YNLVSPKDGVRVSDHNHANVLSRAKGDFYRERHSEFKTTVFDALDTENIRTEKQFSDYL
                     SRFGEVKEYNKGKSDRYFGVKLEGDSKFTRLKSPLFSRQYISDRTIPLLKPTKTQIESY
                     LKEWKSSISHEIKHIHPAGQKIRKEYAALAGEDKQMYLKKVINRYDEKHNLQRQDRRQN
                     SDQSGIKYNTGKFVSGKTIGLPRLPARELVYGLHGRSGGKSINKSQLLLQQNEYGYLST
                     AGRQKVDPDRYVRRLQDRAGRRVVQSALESNIKAAIQRSDDLPFLKQIRTEINAERYLS
                     NLQTRFNIDPSKHTVTYGQDGSPRFRVGNRNMNASDFLTKHLNLEWDEAKEVLLDIWQD
                     QKDNKPFDAVITRVNLTRQQAKERFICLNEHKGWVNNEIKYKHNDNIANYRAELRGLLA
                     IRNKQEREVARGYIIFKKLEREREINSIRKACFREINSYYHRWNPDRRYDMALGDSLKK
                     IIRNPVDGNSISSGNEKEVFSVVKRVQQSQQVQDYVKRELTDLVPHKVSPKETRYLDPK
                     TTQTVFTDKGNHVRVNGEITQDKAEAALLYAKEKFGGMLKLNGSEEFKVACAMAAAEKG
                     MNVILRPEKYHEMMQVRLQELKAEHGHENSVEAERQQQVAGQKPEQEQEKRAEQVNSVE
                     KDTQAKERTPEVAESVRNDDKSRDAVPEQPVKSETVSQKSTSETSVVQEAEKQESVQQN
                     VSAHSEEREQQPQEAQQQKPVTEKDVTTGQDKQTGDEQSKSALATENPHQQNNASLQQL
                     QEEAKVIASQHQEAQRLLNEAVENGAPKEEVDLLSKGVNSISARAQTINEQIKAFEEQQ
                     GQKKAKSVEEKNENTTEKKKRTVNSEKAMEREIERISKQLAKEMNIDKQTVKDTLKFIR
                     FEDVNDLKAAMKNPETVEAVKREIVSYDNALKNERERERNRDNTRSR"
                     /name="LPD7 domain-containing protein"
     gene            complement(34913..35299)
                     /locus_tag="M5T46_RS26445"
                     /old_locus_tag="M5T46_26445"
     CDS             complement(34913..35299)
                     /locus_tag="M5T46_RS26445"
                     /old_locus_tag="M5T46_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_021566153.1"
                     /translation="MKKQDKGKKAVASTSKVERKHVISLRLTDEEYQSFHEYFTSLEVS
                     RSEFFRMVILNRLDDIPTLKQKRPVEYRELLRLFNKSSNNINQIAKKINTEYRNGFISE
                     STYLRYLNVLVNIRDAFVAGINKC"
                     /name="DUF6290 family protein"
     gene            35661..36062
                     /locus_tag="M5T46_RS26450"
                     /old_locus_tag="M5T46_26450"
     CDS             35661..36062
                     /locus_tag="M5T46_RS26450"
                     /old_locus_tag="M5T46_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052962069.1"
                     /translation="MKKIDSLLSPICETIKETNSKSKQAVFNMYLSNILEAKSYYTWEQ
                     ITDYINNNTKTELAKKTYMNMTERAKKRQKCTEQKDLRKSTNIIEKKTDEDNKQRGGVK
                     GFFSTISENKNEIDHDPSATNEKFINKYL"
                     /name="hypothetical protein"
     gene            36080..36835
                     /locus_tag="M5T46_RS26455"
                     /old_locus_tag="M5T46_26455"
     CDS             36080..36835
                     /locus_tag="M5T46_RS26455"
                     /old_locus_tag="M5T46_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="WP_052962070.1"
                     /translation="MKNSINFILQGKGGVGKSFTTAILAQYFIDEKNMDNVVVGDTDPV
                     NTTTVKVKRLNADLIQITENSKVVQSKFDPMFESMLTNGQNTFVIDNGASTFLPLIQYF
                     NDNYVMDMFNEVEQEVYIHTIIVGGQAQADTIEGFEDLVKLVKGTKVKIIVWINEFQGA
                     PALENIPLIKTKFIEKNKDVIAGVVVIQDRKSDAFASDIKNLTEKSLTLKEALESEYFG
                     LMAKSRLKRVFNDIYVQLDSIYDMQHTEA"
                     /name="conjugal transfer protein TraL"
     Typing          complement(9508..10529)
                     /name="IncI2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.902"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(31681..34919)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.815"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgattaatg ataaatcctt taacattgaa aacattattt ctgatatttt taaagaaacc
       61 agattaaaaa taagtaagga cgatcctgtt ttaagcatag tactaatgca tgaaaaaata
      121 ctcgaacatg cactaacaca acttaaaaac agtaatcaaa tagctactga aagattatct
      181 catgacatat cttcaataag agatgcaatc aacgcattac ctgaagctat tgatgaaaaa
      241 acgtcagaac tacaacatgc agcagtagca cttcatgacg aattccagga aagcaaagga
      301 gaaattaaag gttctctgga agaagcacga attaatgcaa cagagaaatt agcagaaagt
      361 gcaaaagaac ttcaattaaa cattacaaaa gttgccgaaa aaacaacaga aacaatagac
      421 acagtaaata aaataataag caatatagaa accgaactat atgacataaa taaaaaagct
      481 ctacatgatt atgtaaaaga gattaagtta gtggatgaaa aaagaaaaag tataacaact
      541 aacctggaaa gtgcaattaa tgacgctttc aaaaaaagcg taaaatcgtt taagttttat
      601 tgtagcgcgg cattaattat tagcactcta ctacaattca caatgtgggg gttctttcta
      661 tataagcttc tgacctgatg ttttaaatgc cgctttagcg gcatggaagg cggcactctg
      721 tagctaaatg tggtttcgct ggttatccgc cgaagccccg cgctagcggg tgctgaattc
      781 tatattctga ggaacagcgc tcgcgccccc tgctaagcca aaaacgcacg aaaccaacag
      841 ccactcgatt gtttctggtc gaagactgac aatgttaaag cgacgtgaca aaaatatttg
      901 agcaactact caagaaaatt cggttttgaa attgaacaat ggtctgattt cacaccgaca
      961 atgcaatatt aatctgccgt tacaacgtca ctgaaactct gctctacacg ctcgtaagcg
     1021 gccctgacgg cccgctaacg cggagatacg ccccgacttc gggtaaaccc tcgtcgggac
     1081 cactccgacc gcgcaccgaa actctgtcat ggctgaaagc gggtatggct tagcaggatg
     1141 gggataggta aggtgaaatc tatcaatcag taccggctta cgccgggctt cggcggtttt
     1201 actccagttc cacatgtaac aacggagtgc cgccttccat gccgctgacg cggcatattt
     1261 agtgtgtatt attacacaat gttgtttatc aggctgacag ttctcaaaat tgccctaaaa
     1321 aaaccagtaa cgttcgccgc ccccaacgac ctaacgttag tgagtgggcg aggaagcgga
     1381 agactgccag gcctgaattg cgcttgtgag aaggggccac aagccccttc ccgtctttca
     1441 tggtaaatca ccacaaccga gaggacagca atcgcgatca aagccgtgac aacaatagcc
     1501 attggcatag ccgttgcgta tctcttcagc aatctctttc gcagaaagat aatcaaattc
     1561 cttgcgagcc agttcaaacg cctgggcgtg ttgttcttcc ggcaatctgg cgacaacttt
     1621 atcaatcgca gcactcaaac gacgttggta tagctcaaaa acaagaccac aaccaggcat
     1681 gatctctttt gataaaatat ccagttctga gagaaatttt tcactatcaa aagtatccat
     1741 catcttcacc ttcgtaagtt acctgacatt atttctgcat cagttaataa caggggctat
     1801 taagcccctg ttatatttat tttccttcta tagcgattct tctaagctca tgaaggatac
     1861 aagccgtttg atctaccaga ttttcgtcat gaatgatgtt ttctgctatt aactcgttaa
     1921 acgctacgac cagaaaatat gactctctat aaacgtcatc ctgatcaaat tctgccgctc
     1981 tatactttga atagatgata atgccgcgat cttgaatttt ctgaatttgc ttaatcactt
     2041 ctgaagtcat ttttaatctc ctgtccgttt ggacttaaga aggaaaggag cctgtcccct
     2101 ttcgatgaaa caataataca tctatttgga tgtattatca agatttctgg cctgaaaaac
     2161 ggtaaattat ttggtggttg cacgcttttt atccgcgcca gcggatggaa ggggcgaacg
     2221 aaaaaaccag gggagcacaa gagccacatc gccccggggc gcggcaacgc cgctactgtt
     2281 atcaaccgga accagggata ccctcccgcc ctgctaagcc cgactgactc acagacaacg
     2341 atgacgctga tttgcgccgc agaatgccgc agttagcgct cgcgccagcg agtttacgcc
     2401 gtccagggtt tacttggacg gggcgctgta ctgcgcgtgt gagcgtcagc gagctacggg
     2461 tgcaacacac cagaagttaa accgctctta atatggtttg cattaaaatc tttcttgacg
     2521 aatcctcatt aatatcacac ttgaaggaaa ttatttttat tgctctcttt ttcagagcgc
     2581 tttttatttg aactgttgat aataaatcct tgtaaatcgg atgaagataa accccatatt
     2641 tatcttcaaa agctctatca atttttgatg cattcataat agcatcaaaa caattatcat
     2701 catccacagg gcaagctgtc caaaacatca tttcaacagc cattttctta gcttcattaa
     2761 tcatcatccc cccttttatt acttaaggcg actatagtta catatagcac ttttgtctca
     2821 atatatgaag gggcggaaat gccccttttt atttaaaaca tttcataccc ttgcaagtag
     2881 tttaaccacc accgaaattg atcccactca ctaaacgtca ttgctggaat tctggtttcc
     2941 cagcctttga caagagaatc agcaagctgt acgatctgcc gagttgacat ttaaaaacct
     3001 cattattcag ttttgattgt cgataacgtg atcaggttgg tggaacaacc tgatcacacc
     3061 ttgaatagtc agtcattacc ctccttcttt tctatagtta ctgtgtatcc gtaaagggtc
     3121 aaaatgcgat aagcatccaa cgcctcacgc tcacttttaa atgttctgac ttcgtgtaat
     3181 ccattacctt tatcaaggga tacacaaaac tcataatctt cttctacttc tatcttttta
     3241 attaatttat caagttcggc taatgttcgt tttggctcaa aaagaaatgt gcattgtaca
     3301 atggcgttta aaccttcccg atcaagctct aacagagcat cattataagc agctttaaaa
     3361 cagcgattat tacgcagatc agatgcaaac tgcattgcgg cttgttttaa cgttttcatg
     3421 aatttccctt gcattgatgt taatagacaa tttcagaatc aatattgaaa tttccagaaa
     3481 atgcagggtt cccccctgca tttataatgc ttaacgaagg cactcaataa aatctttaaa
     3541 actcaagcat tcatcgccat cttttaacga atcaaaatat ttttcatatt cgtccctata
     3601 gaaaaggact aaatcaacat cactctgctt aagttcgcca aactgaggaa caattccggt
     3661 tttatagtca cgaattttat tgttatcagt aaatatgaca acatactgtt gtcccgtatc
     3721 tttatggata agggcaatag tgcaaagtgt tactcgttct aatgtgaaaa catagtttgt
     3781 ggttgaggta aaaacaggtg agcatttcat ttaaaatctc ctgtccgttt ggacttaaga
     3841 aggaaaggag ccaatcccct ttcgatgaaa caataataca tctatttaga tgtattatca
     3901 agatttctgg cctgaaaaac ggtaaattat ttgatgattg cacgcttttt atccgcgcca
     3961 gcggatggaa ggggcgaacg aaaaaaccag gggagcacaa gagccacatc gccccggggc
     4021 gcggcaacgc cgctactgct atcgaccgga acctggacca ccttccccgt cctgctaagc
     4081 ccgactgact caaaggcaac gatggcgctg atttgcgccg cagaatgccg cagttagcgc
     4141 tcgcgccagc gagtttacgc cgtccaggaa aacctggacg gggcgctgta ctgcgcgtgt
     4201 gagcgtcagc gagctacggg cgcaattgct cattgatttc gttgtgcgtc atgttgcccc
     4261 ttttacaaag gacgtattca gggtgttctc cagcaagcaa taacagatac tcaaattcac
     4321 cttttgacaa atttaattgg ttgtgggttt ttgcttcctt tttctgccat gtacttaagc
     4381 ctacgccaaa aatttcaccg cattctgtct gggtaaggcc aattttttta cgtaatttct
     4441 ttatgttttc cggtgtattt tcaatagtca tgtttcattc tccttatatt ctttcatcat
     4501 ggttaaaaga atactcttat caacaggttt taaaccattt tttgtaaaat ctattgcccc
     4561 aacttcattt tcattaaaaa ccagttcgct taacgttctc tttgtcgttt tagtttttcc
     4621 ttctgtgtac acctttgaat agctgcttat tttttttaca aaatgatatt tttcatcaat
     4681 catttcaaga taataagtca atcgcccttt gttttttctc ataactgttt caaatatcct
     4741 gtaattttaa caagttgatc tatgttcaag ccccgaatct gcaaatcata acgctcgccc
     4801 tgtttgatga ctttaccgtt caatccgttt tcaactttaa agcctgtcaa tgccccatac
     4861 cagctttcaa gctggacaag ttgcgataac atacctttca ggatcagaat ttcgtccttt
     4921 tttacctcct ggcttttctc atactcaatc gcctgtaaca cctcctgcga agcatttatc
     4981 agaatttttc gctcgatatc ggctgctata tcaacgggat tccgcttcct agataacgtg
     5041 caaacatgat gcggactacg ccaggaacga ctgtccacac tccccacaac agaaaatcgc
     5101 tctttttcca tccgaatatg cacggagtaa tttttaaact gcggtgatgt cagttttata
     5161 cgagacggac aatcatcaag tttgttcacg cgccagccgt tccccaagat acggcagaca
     5221 acttcaaaga cagggtgata ttgttcaaaa aaagagttca tctgttttcc tttttgctac
     5281 gtggcagctg ttcccgtctt ttggggttcg atgccctaaa agacgagctg gttatgatat
     5341 cgggggcttc tgcccccgtt ccggttacgc tctgttttcc acgcgttgcc accctgtcgc
     5401 cctgcgctgt ccagtcgtac cgtattttgc gcggatttct tctatcgtgt gttcttcacg
     5461 attccagtaa cgtttatgtt cttctggacg ataaaaccat tgttttttct ttgccgccca
     5521 tttacacccg atttctttta aagtttcttt atgtgtaatg gtatctccac taatccagac
     5581 ccagttacct ataacttcaa ataccagacc tgataaacca gaaagaacgt ttaatacttt
     5641 ttccaggtcg tcaccgtaat tatatcgaga atgttcatca gtgctctgaa actgattaat
     5701 tttatcgatg ttagccatga gaaaatcaaa agctgcattt accgctttca tcagttcagc
     5761 ccctaaagga ttacgatctg gatgatattt taacgcagct tttctgtatg ctgctttgat
     5821 atctttttcg gttaattcac cggataaccc aaaaacattt aacgcttcct ggatattcat
     5881 ttcaaatctc ctgtccgttt ggacttaaga aggaaaggag ccaatctcct ttcgatgaaa
     5941 caataataca tctatttaga tgtattatca aggtttctga tctgaaaaac ggtaaattat
     6001 ttgatgattg tcacgctgtt atccgcgcca gcggatgtaa ggggcgaacg aaagttccag
     6061 ggtagcacag gagccacatc gccccgaagc gcggcaacgc cgctactgtt atcaaccgga
     6121 accagggaac accctcccgc cctgctaagc ccgactgact cacagacaac gatgacgctg
     6181 atttgcgccg cagaatgccg cagttagcgc tcgcgccagc gagtttacgc cgtccagggt
     6241 ttccctggac ggggcgctgt actgcgcgtg tgagcgtcag cgagctacgg gcgcaacacg
     6301 ccagaagtta agcagccctc attggcatcc ccacgacctg aaggacgtgg ggttacgtcg
     6361 catttgacag ggattcccca tgtaaatctt aaagattcct gttgaacatc ttaaatactt
     6421 ctgtagtata cctatagtaa ttacctaaca ggggagacag taatggctat ttatgcaata
     6481 tcaatatcaa aaggtggggt gggaaaaacc acatctgctg tacatataat cgatgaaata
     6541 aagcctgacg ttgttttcga tctggattta caccacagcc tgagcataat taacagactg
     6601 cgaccagctg aaaaaaaatg gtcaatagtg acagtttcag acaaagcaga attgctcaca
     6661 tatctgaaaa aaataaatga cgaaggaaaa acggcagtta tcgactgcgg ggggtttgat
     6721 gctgatatta accgcatcgc tatagctgtt gctgatgtga tcatcgttcc ggcaaatgac
     6781 gatgttactg agcagatcgg acttgccacc ttcgatgccg tcctcgccga aatatccaga
     6841 caaacaggca gaaatataca ggcccatgtt ctgatgtgta aaacacaacc taatcagaaa
     6901 aacttccctg atatggacga tacaatgaaa aaggttaaac atatggcgct actgaacgct
     6961 cgcttatcat atcgtaaagg acgctatggt tttaccgatt ctttgcgtaa aggaatgggg
     7021 ataacccaaa ttaagcatgg tcgtagttca caggcaggac gagaagttat agagcttgta
     7081 aaagaattac gcgctttatc ttcagataac acaggttttc ctgcataaac cccaagagaa
     7141 ccttaaagaa acacaaagga tttaccatag ataccttaag gattcataaa ggatttccgc
     7201 atgaaaaaca agcaatccat ttttgatgcc gctctcaata ttggtaacga agcgccttta
     7261 caggtaaaaa acgaaacgcc aaaacaacgg acggtcagaa caaaaacact aaaagccata
     7321 ccgacaagat attttgatgc acacctgaaa ttaagagaaa caaataaaac gaatctcgat
     7381 ttttccaatt acataatgga agcactacgg gaaaaactgg aaaaggatgg cggactttaa
     7441 atctaaaggg aacacccagg aaacacttag gatatctgac ggaaccccca tgtatttcca
     7501 taagtgtttt tcggggtgca agcatcgttc ttgcattaca gtatttcatg aactacaatg
     7561 tagtaacata cctaccagta tttgaggcaa gaatatgaag attacaactc tatccagtcg
     7621 ggaactgaat caggatgtaa ccagggccaa acgcgccaca agtgatggtc ccgtatttat
     7681 tacggatcgg ggtaaaaccg cgcacgtact tcttagtatc gaagaataca ggaaattaac
     7741 gcaacaacgg cgaactctta ttgacatcct ggctatgcct gatgctgatg atgttgaatt
     7801 tgaaccgcag cgaattaatg ttggtttaaa acctgcggag tttttgtagt gtatattctt
     7861 gatacaaatg ttatttctga gctacgaaag gcccgtacgg gacgtataga taataatgtt
     7921 gcaaaatggg cattgcatac agacgcggca gatttctttg tatctgttat taccataatg
     7981 gaacttgaac agggaatatt acaggttgag cgtcgagata tacgccaggg gattatgtta
     8041 cgttcctgga tggaccagca tgtgttacca gcgttttctg ataagatact tcctgtagat
     8101 actgcagttg ccttacgttg tgctcggtta catgttcccg acagacgcag tgaacgagac
     8161 gcattaatag cggcaacagc tctggttcat gggatgaccg ttgtcaccag aaatgttggt
     8221 gattttgaat taagcggagt atcaattatt aatccctggg attgatatac cttccctttt
     8281 ttagatgctg caagattaca caaagaacac aaaaggaata caaaggattt ccataagaca
     8341 tctatagaat tccttttagg aatccaataa aaatcccagc gacgacacat taaatgccaa
     8401 gtttgcggcg tggactatac agtgtgtgat tcaccactat gaatcctttg ttgatccact
     8461 gtgttacctg cgcaggttga actccggtca gcctggcgaa cgatgcctga ttcccccgat
     8521 agaacttgtt gatgtattca atcaaaggca ttttttaatt ctccgcaaca atacgaaatc
     8581 atatagtaat ttactatatg atttatcttt ggcaagtaat taacttatct gatttcttcc
     8641 attggtaagg gggcttttga tgtcgcgcca gcgacatgta aggcggtacg ctgtggttac
     8701 atgtggaact ggagtaaaac cgccgaagcc cggcgtaagc cggtactgat tgatagattt
     8761 caccttacct atccccatcc tgctaagcca tacccgcttt cagccatgac ggagtttctg
     8821 tgcgcggtcg gagtggtccc gacaagggtt tacccgaagt cggggcgtat ctccgcgtta
     8881 gcgggccgtc agggccgctt acgagcgtgt attcgatact tccagccaga ggactgacag
     8941 cgacgatgac gcttagttac aacatccatt attaaaagcg atgttgttcc cgccctttgg
     9001 gcgggggcgg cggcgctttt cagtgatggg ttgggcgctg tggctgagta tttgcagcgt
     9061 tttttgtagg agggttggag gggctttgtt tttcatcggg cagaagttca tatgcccgcc
     9121 gcgaagcggt gggaggtact tttatattct gagcggcgcg gctttagccg ctgcaagcga
     9181 aggcattttt gtgattatcc acagttgttt caaagataaa tattttccct gcggcgaagc
     9241 aacgctgggg attaaagaac aggggttaag aatatattgc aggtttgatc ttctatgttt
     9301 gctgctttct gaagttattc gtttgcggtt ttttgtgaat aaccgaaggc ggtttagctc
     9361 cgatacccga ttgtggccat caaaaaactg tcagagcggg acaaaatgaa aaacggtcgt
     9421 caaaaatgag gaatttcatg aaatgcaggc aacacgaaaa gccccgaagg ggctgaagag
     9481 agtgccgatt gtatcaggca gctaaactag tggggtggcg aagctgtcag ggccagttca
     9541 agctgacgta gatccctggc taccagttgc tctaatcttt ctttagtgca ccagtacgct
     9601 tcgtccggcg acatacgctt cagaataaac cgtgcaattt cgtttcgctg ttcatcatac
     9661 ggcagttttg caagacgatt ggcacgtttg cgctttgctg ctttctctcg tcggaacttg
     9721 atggcatcga gagaacgctg cgcataccag cgtttacgag ccgagtgaac agagatttcc
     9781 tcgccctcgc ggataacgcc ttctttaatc agttgcagac ggatttcact ttctgcaagg
     9841 cgcttacgtt gctgttcctg aagtttcacc agatcaactc ccagcatttt ccagccagtt
     9901 tcggtgatcc agacgtattt cggcagtcgc tggcgggatt cacggtccca tatcgtcttt
     9961 tctgacgttc ccaacgtgcc aaaggaaacc tgctctgcca gcagacggga aaggcgcgat
    10021 actgtgactg cggtttcagg aataacgttt cccttactgt cttttgggct tatttcttct
    10081 gccaggcggg agacagacat gccgacagtg tgcaatccac gatcacaaaa actcacaagt
    10141 acaacccata ttgcgtcaag cagccgctgt ttctccggtc ggaatttata tttgcgaccg
    10201 acactctgac gtttcagtac ataaaatgga tgcaacgaca tatgcattct gagtgtgtac
    10261 tgaccagtga ccgggtcttt tttaacgaga cgattgtaag cgtggcccaa ctgtccgccc
    10321 agcgccttat agctgtcggg acggaaccag gctggattct cacactttgg cttagtggaa
    10381 tgttcaccac ggcggcgctt cgtgctccgt ttttccggtt caaccagaaa tgacgtggcc
    10441 ctgcccgatt cgtaatcctc ccagaaaagg atttgctctt cgggagacag ttcactccag
    10501 tgtaatgaag gcctgttcag ggaagccata agcaatcgct tatggcagag cttgattcgc
    10561 acagtgcatt tttacaccgg acgatattga ataatgagga aagcatcatg tatactgtct
    10621 tccaagggat ttcccctgcc cccattaaac tttgatcggc ctctgccaag aaacggatgc
    10681 aaagttaatg ggggtttgtt ttttatggct tcatggaatc tttcaactcc tgccatacac
    10741 ctgaatgcct tatggcgcat cctaccttta tcccctatga cggggaccaa atttctcaaa
    10801 tttaagcgaa gatcaaaaag gatcaacacc gaatgccgct taatatacct gtagccctcc
    10861 gatttgaaaa gtgtttgtcg tgacgctcat tccaccaatc tcgccaaatc agcacatccg
    10921 gcactgccga tgaaacaatc aaaaagtatt ttttttcgcc acttctttaa tttttagaat
    10981 attagatcgt gaatgatcta ttcaacgatc tatcatacca atataaaagc tgattttaga
    11041 attttatact ttttattttg atttatgcgg gataatagat cgcctaaacc accttgaacg
    11101 atctattgta ctggtttaaa gtctccatgt tgaagttttc cgctatcagc agaacgcccg
    11161 aacaaggcgt tattcgtacg ctctttaaca cccctggaca gcggtaattg cctgaagccc
    11221 tggcatgagg aggattaatt gtgtgcaaaa aacagcactt gctgacaatg ttcagtgtgg
    11281 gctacgtata gagtgacgtg ccgcgccatc tgacaggtaa cgactcattt ttacaatttt
    11341 caacatagtt aattcccttt atcaaactcg attccctgaa agggggatta actgtgtacg
    11401 aaaagtacag gagataataa tgagagatga taattatctt gatggattaa ttgccggagg
    11461 tactgttgca atttcttttg cgggactgat tctgttaatc gtgagtaatt tgaaaaacga
    11521 agctgagaca gaaaaaactg cattttatga atgggccgat tcacactgtg aaaaaattga
    11581 aaaaaatttt aaatgttact gaaaattcga aaacatttaa gtgtgatgac ggaaaaattt
    11641 acacaataaa atggtgaaat agattaacat gttatgtcac aagcaaaagc aaacctgaag
    11701 aaattacgtc atcactgtta tattccacca aaacaaaaaa tatccagact gtattatgtt
    11761 gaaaaagcct taagttctcc ctggatatat cgtgaacggc ctgcatacag agcagagttg
    11821 ctacttacac atgacacact gcttgttatt tatggcctgg caaaatccgg cctgattgac
    11881 cgttctgagg aatcactcaa cagagaaatc aaaggggtcc gaaaagcgct cgcagccgga
    11941 cgtttccgtt ggtcgtgaga tgccgcgcca gcggcatgta aggcggcact ctgtacttaa
    12001 atatggcttt gcgggttatc cgccgaagcc ccgcgccagc gggtactgtc ttctatattc
    12061 tctggaactg cgctcgcgcc ccctgctaag cccaaaacgc acggaaccaa cagctacacg
    12121 gttgtttctg gtcgaagacc gacactgctg aagcgatatt attcgaaagt tatcctactg
    12181 cgcaatgccc ctgttttatt tgaggaaaat ggcatgaagc ataaaaattt tattccgaaa
    12241 tggtatgatt caactcttga gtcaattccc catcaagatg ttaacacgat tcacagtatc
    12301 attgatgaac accacaaatt atggaatatc cagatgtgca acaggaacta tttaacagac
    12361 tgttatccgg taaaaaactg acgattcatg atgagcgaga aataattgat atctgctcac
    12421 gaaggagcgc aacatatcat ttaaccagaa gtaatatccc taagtaatct gacgctgcca
    12481 ataatccgtt aaattaaact ttcatcgtta cctgtcaaaa aatgtctata tccgcaactg
    12541 tgcttatgac actgatcagt cagtgcgcac ctggtgtttc tcctgaaacc atgcgtgcca
    12601 ttattatgac tgaatccggt gggaatccct acgctatagc aaatgtcacc gacggtggca
    12661 gtaaatattt taccactgaa gaggaggctg ttaatcacgc aaaaaaactg actacagata
    12721 aaaaaaattt cagtgctggc ctagggcaga taaacagccg taacttccag actttaaatt
    12781 taactcatga atccgttttt tctccctgtg cgaatattcg cgctgctgcg gcagtgctga
    12841 aaacgtgctg ggataaatac gaaacaaaag ggggggacca acaaaagata ctacgtgacg
    12901 ctatgagttg ttattactcc ggtaataaca aacggggtta tgtgaaggaa agcaatggca
    12961 aaagttatat cgatataatt gaggaaaaag ccggaataca tactcattat gatattccgg
    13021 caataaagtc atattcagaa acatccagcc atttaatggt atccagtgat aaccatgata
    13081 aatgggatgt ttttggtgat ttcggaatac aggattttaa ggagtaacac acagccatta
    13141 atttaattat tcctaccatc tttaactaca atctaattaa tgttatctaa ttatgtatgg
    13201 agataatcgt gaataaatcc attgcattat acagaacgtt ctttttattt ttactttgta
    13261 gctttattgc tacaaatccg gctctggcag cgggtgttgg tgggcttgat aaaggcacaa
    13321 acttcatcgg tgaactgcgc ctctgggcgt atggttttgt tggtggtgtg gcccttttac
    13381 acattctctg gaaagtattc caggcataca cggaaatgga ctcctgggct ggcgttctca
    13441 aatcactgat gtatgccgca attgccggtg gttgtgtggt agctgctgaa gcctgctacc
    13501 agattttcaa ataattacct aatcaccgga atacatgatg tattccggtt catcatgagg
    13561 atcaaaagtt ggataaagaa gaaattaaag cagccgaaga gcttaaggca atctttctta
    13621 cctacaatgg cctgaatcgt cctgcaatga tcaaaggtat gcccatcatt ccttttattc
    13681 tgtgtctgct ggctctcatg gtttcttttt ttgttggcat actgactatt gatttttacg
    13741 ggcttattat tccctccatt attatcggcg taatgatcgc catcaaacaa atgtgtgccg
    13801 atgacccgaa cgccatgtct gccagggcac taaaatttaa aggtctgtgc cttaagggat
    13861 ttcgttcgaa taacgttctg aaggttgaat aatatgtcga taaagaaaat aactaataat
    13921 ttcattcctc cttatcactc acatattcat gaaaatatca tctggcttga agataaaaaa
    13981 ctactgctca cttttcttat tgaaggcatt ccctatgaaa gcctgacaga tgatatgatt
    14041 ctgaacaact ttaacggaca aaaagaaaca cttcttggac tctgtaaaag tgaaagagtt
    14101 tttctgtgga aacatcttgt aaaacgcgaa tcacctatgg atgccagata taaatttcct
    14161 ggcaatcctt ttcttcagcg cttaggtgat ttctactgtg atacttttaa cggagaaaga
    14221 tcattcagaa cagattattt tctcacaata ggcattcctt atgatgatat cgatgctggc
    14281 gaaaaaaaag ctaaagatat catccggcag attgaaacag gttttaaaga ctacaatatt
    14341 tatacgcttg ggatcagtga cggcggtatt tccgatattt gttcaaacta tctgaattat
    14401 ttactcaatc atgattttga gaaaataccg ttatctgcaa cgaagatttc agagagtatt
    14461 aagaacagca aattctattt cagctttgat actcttgaaa taaaaaatcg cacaggacac
    14521 gataataaat tttgttccaa ttatatcgta aaagatgtac cccgaggctc gcagattggt
    14581 cagtggaatt ttctgttatc cctgccattt gagtttatcc tgacgcaatc ctttattccc
    14641 gattcgctat caaaatccag caagaaaatt gagcaacagc ttaacaaact cacttccgta
    14701 aaggacgctg gtaagacgca acacaccgaa ctccatatgg cccaggaagc tatcatcaac
    14761 caggaaaccg tttttggctc ctggcacagc gtcctttccg tcttcggaaa cacacctgat
    14821 gaagccagtg ataacggttc tgaagtcgcg gcggaatttg tcaccagtgg gcggggattt
    14881 cgttttgttc gtgcagcgtc tgaagcacct tatgcatggt tcagtcatct gccagcctca
    14941 aaactgcgcc cccttgccac acaacgcacg ctcactaatg tctgctgcct gatgccgctt
    15001 cataatcaca gctatggcaa aaaatcagga aatccaattg gtgatggctc tgccataatg
    15061 ccgctgaaaa ccgtcactga tggtctttac tggttcaaca ctcactacag cgcaccaggg
    15121 cgtaatgtaa caggacagat gatcgcgggg cacggaatgc tgctgggtgc gactggtgcg
    15181 ggaaaaacca cgtttgaaat gatggccgcc gcgtttatcc agcgttttaa cccagatatg
    15241 ttcgtgattg acttcaaccg ctctaccgaa ctggcaatca gaatgttcgg tggtcagtat
    15301 ttctctctgt ctgagggtgt gtattccggc ctgaacccct tccagatcgg agacaatgac
    15361 gatcctgaac tgatggcatt cctgaaagag tgggtcaaac gttgtgctgt taataacgat
    15421 ggcagcgact gtagcgacag tgaggctgaa gagattgacc gcgccgttga aatggtgatg
    15481 aaacttgaac gtcgccagcg acgtttcgct cgtctgcaat cccgtataca aagccccgaa
    15541 ctgaacgtcc gtctgtctaa atggtgggat aatggcgctc ttgcctgggc tactgacagc
    15601 gaagaaaata aattcgatcc caatcaatac agcaaaatcg gttttgacac gactgtaatc
    15661 ctggaaacag ttaatcaacg agatcatcct gcgtgtgaag tcattctttc tgttctgttt
    15721 ttctacaaaa aacgaatgca gaaaggcggt aagctcatgc tctccatcgt tgaagagttc
    15781 tggaaaccct gtaactaccc aatgacacag gctttaatca aggcttcgct caaggctggt
    15841 cgtatgaagg gggaaatgat gtggctcacc agtcagtcac cggaagacgc gattaactgt
    15901 caaattttcg cggctctcgt tcagcagaca ccgacaaaaa tcctgctccc taaccctgat
    15961 gccaaataca gcggctataa agagattggg ctgacagaaa aagaatttcg ccagcttaaa
    16021 aagctgaaac tcgaaagccg aacaatgctg attaagcaaa gtggctcaag tgtctttgcc
    16081 aaaatggacc tggtgggatt tgacgatttt ctgcctgtta tttcctgctc aatgaaaggg
    16141 attaaattgt gcgaaaaaat cagggaggaa ataggctctg acgatcctga actctggtat
    16201 cccccgttta tcgaggggat ttatcagata gatgacgtta tacgggaagc taacagcgat
    16261 atccctgaag tctggctgcc cttcatgatg aaacggctca aagacaaatt gccggaaaaa
    16321 taactgtctc tgtagcatgc cgcacaagcg gcatgtaagg cggcactctg tactttaata
    16381 tggctttgcg ggttatccgc cgaagccccg cgccagcggg tactgtcttc tatattctct
    16441 ggaactgcgc tcgcgccccc tgctaagcca aaaccgcacg aaaccaacag ccacgcggct
    16501 gtttctggtc gaagactgac aatcttaaag cgagaggaaa attcaatgaa aaagactctg
    16561 acagccctgt ttctgggtgc caccctgacc ggatacagtt ctgtggcaag ttctgtaggc
    16621 ggtgtcatcg ttaaagaccc tggcagcata gctaaaaccg tcgaagtaat taagaaggct
    16681 gccgatcaga ttgctgaact gaaaaaacag gttgatctgg caaccagcca gcttgaagcc
    16741 tataagcagg aagtgctgga taccaaaaga cgactggaag gtataaccga ttattccgcc
    16801 atttttggca gtgctgaatc ctacatgaaa gacttctggg aagatatgaa gaaggaactt
    16861 accgatgctg atattcgcag tatggcaact aaatacggct ttgatacaaa agagtacgac
    16921 cggatcaaac gccagtacga atcaaagttt gaggaaatca cgaaatacga agcgatgagt
    16981 aaggacctgg agaaaagcgc agaaaagata aaggcaactc aaaaggcttt cagtaaagcc
    17041 gatacgccac aaaaacgcga agagcttcag aataacattc ttctcgaaac tgccgccatg
    17101 cagttaaaaa ttgcacagaa tgaggcagaa atcaatcgta tggcccgtga acgtaaatta
    17161 cgtgaggaag cggcactgat aaaatataca gaagataact tttcctttaa ctgataagaa
    17221 agtcacaaat tctctcctaa tacatacact tttcctgtac tatttgatag catatttaat
    17281 taatatatgc tatcaggagg taacttatgt tcaaaaaact acttggcggt tttatccttg
    17341 cctttgcttt tttattatcc ggttgcggtg gtgataatgg tgaattagcc gggacatata
    17401 tagaaaaaaa tcctgactct ggtgatgcac ctcaaaaatg gattatcaaa tacgacaaaa
    17461 aggataatta ctacactcaa atatggatag aaaaagataa gaaaggtaat gagcataacg
    17521 gaacaaccaa ctcattcctt attagagatg gagactggct tgcaggaaaa gaaaaacatt
    17581 ttattcgagt gattgataaa aacacgcttc agttaagaga tgatgagaac gctttgtata
    17641 tccgtacaaa ctgaatgctc tctgattaac ccacaacgcc tgtccctgtg acgggcgttt
    17701 tcttttgcag gtaataacga tggcttcaaa tgactccgct tttgcaaaag cgaatttcga
    17761 aatcgtccag ccgatgttca atgaattatc ttcgattatt cagaacagcg taaccacaaa
    17821 ttttcagacc attatgcaac ttgtgactcc attgttcgga gccgccgtca ttctctacgg
    17881 tgttttcttt gcgtgggaaa tcatgtacac acgcaaggat gatgtgatga tggaatccgt
    17941 aaagcgtatg ggcgttctga cgcttgtttt tgccatgagt accgccggag gtatgtacct
    18001 tagctatatc gttcctttcg tacagggaag cggtcaggaa gtggcctcca ggcttattgg
    18061 ccaggacgga gccagtaccg gagccgttat cgatggcctt cttaacagac tgatagattt
    18121 aggccatgaa caatttaagg tttttcaaaa agctgatggc tggtttgatt caatgggggc
    18181 atggattgtt tttattctta aagccattct tcttgctctt gctggcggcg gatttgttat
    18241 ttactgcgca gcttatttat taatggccga aatgatggtc ggcatactgc tttccattgg
    18301 cggtgtgttt atcttatttt ctgcgtggcc taccacgcgc aatatgttta cagcgtgggt
    18361 gggatcatgt tttaactata tatttctcaa tattgcctat gcctgtctgt tcaaggttct
    18421 gattcagtat attaataact atatcaatac aaacagcgat gatattacta aaaatatctg
    18481 gagcgtcctt tgtgttgcgg ttgtattcct gattggtaaa ttcctccttg ctcaaatatc
    18541 aacgctgata agtacattaa caggcggtgt gggtattaac ggtctgacaa gcgctaccgg
    18601 agatgttata aaatctctgc gtggggctgg caacagcaca cgaaatcagc ttgggcttgg
    18661 ctttaataaa acagcacagg atgcacgggc gcgtaacagt gaatatgctg ccgcagcaaa
    18721 agattattta tcttcccgct ttggtggaaa catcaaggcc ggataattcc ccctctttac
    18781 tcaatcaata tctcttattt atccggctta aaaggtatgt tttatgtata aatatatcat
    18841 tccggcagtt gtctgcttat ttctttctgc atgtaccgca acgaatcccc ctgaagcacc
    18901 tgaagctaca ggaaaatggg agccattata taccagcgtc gataacatta tgaagggata
    18961 taaataatgg gtttgaaaga atattttaaa agatccaaag cggaagcacg taaaacaccc
    19021 gctcaaaaag aaaccgataa aaaaattgaa aaaaacgtac tggacgcagc aaaagaattt
    19081 gcgcgtgatc gtgataacga actgaagcgc tcactgcgga tcacgcgttt tgcgcttttc
    19141 ttttcgtccg cttctgcggc tgcaatggcg gttgccctcg cagtcatggc cccgctgaaa
    19201 acaacagaac cgtttctgtt acgggtagat aaaacaacgg gctacacgga tgtgatcacc
    19261 ccgttcagtc aggattccgg tacatatgac gaggcggtaa gccgctattt cctcacgcgc
    19321 tatctggaga accgcgaagg ttacgaatgg tacacaattc agaacatgct gaataccgtt
    19381 gaactgatgt cttcctccca ggtattcgcc gaatacaaca acatgattca cggcccgtta
    19441 tctccgctgt ataagctgaa gaaaagctac aaaattcttg ttcgcgtgca gtccatcacc
    19501 tttctggatg aaaacacggt acagggacgt tttgttaaag caatcaccga tcccgatggc
    19561 aaacccgcac ctggatacac gccaacacaa tggattgcca ccatcaaatt tgtatggcta
    19621 aaagacaaga tcgcgactga agctgagcgc cagataaacc cgttcgggct ggatgtgctc
    19681 tcataccgtg ttgatccaga ggtggtgaaa tgatgatgcg aaaactcctt ttatccctgg
    19741 tcatatccgg tatcaccatg acctgtcatg ctgcaaaaac gccagcgtcc gcaggttttg
    19801 acagacgcgt acaacaaatc gtctataacc ccaatgacgt aaccattgtc aaaacaaaag
    19861 cgggagaagt aacccttatc gagcttgaag agggagaaat catcaagagc gaagatactg
    19921 gtcttgctat tggtgatcct gaagcctggg atccggctgt caggggtaac actatttttc
    19981 tgcgtcccaa agcagcagaa cctgatacca atatcgcaat tgtgacaaac aaacggatct
    20041 actccatcca gttagtcagt accgaccaga acccgaccta tttattgcga tacatctatc
    20101 cgaaaccgcc ggaacccgtg aaacccaatg tcatgacggg ccggagtcgt gttgtcgcgc
    20161 cctgtactga agggaatatt gtaaacggtc gttatgaggt gcgtggtagc aggaaaatta
    20221 aacctgaagc catctgggat aacggtcgtc tgacctgttt ccgctggact ggcgcagctg
    20281 accttccggt tgtgttcagg atcttaccgg acggtaagga gcaactggtt aattaccata
    20341 tggataaaaa catcatggtt gtgcacgaaa tttcgccagc ttttgtactg cgtctcggag
    20401 gtgaggtgat ggaagtcaga acaaaccgtc atataccgcg agcctggaac gccagcggaa
    20461 cactgactgg cgaaaccaga gtggagatta ataacgatga aaaataacaa cgatattgtg
    20521 attgaaaaac tcgataaaat tgacacgcag gaggcggata ccgacgcaca aaaccggaat
    20581 ttcatccact ccggtaaaaa acagggaaac aaaaaggcaa aagcggtcct tatacttgca
    20641 ggttttgctt ttgttaccgt agtcattctg acagccgttt tatcaaaggt gttttcatcc
    20701 tcgggagagg aagaaactac cgaaactgcg accacaaaag cacaggcaga ggtctatgca
    20761 caaaataccc aggcaaaaaa tcttgatgac ctgaaagaaa gcgtcgccag aagcatagag
    20821 gaagtcaaaa acaacgttaa ttcagaagag gaacaagcca gggaagaagc accaccggaa
    20881 aacaaggaaa ataaaggtgt cactctgcca gcagaaaacg aagctgatac cggatataca
    20941 caaagtaatg gcgatgcccc acccacacca gaacagcgtc gtctgatggg aagcgtaatg
    21001 gttgatgaca gccggatcac acagtcacag cctggaagtt ccggtcaaaa aacggcaggc
    21061 gctacgccag cgtcagacaa cacaaatgtt tctggtgatg gtgatttttt acagggtgct
    21121 gactttgctg atggctctgt atcccgcata aaaaatcgtc gctttctgct ttcggctgga
    21181 acaacttttc cgtgcgtcct gaaaacaatg cttgtcacca catatcctgg tgtaacgctc
    21241 tgccagttaa ccaaagatgt gatctcggat gacgggaaaa cggtattaat ccgtgctggc
    21301 gcacaactcc agggagaaca gacaaaagcc attacacagg gaatggcacg cgtgtttgtc
    21361 aactggacaa tggttaagga ccgcaatgtc agagtgcgga ttaatgctct cggtgctgac
    21421 agcctcggcg cgtcggggct tccggcgtgg gtggatacac atttctggga gcgctttggt
    21481 ggcgcattaa tgctttctct cattgaagac actctggctg ccggaacctc acgtctttcc
    21541 cgcgaaaaca acagcgactt cactatgaac aataccaccg gaacgtccag tgaactggcg
    21601 aaaaccacgc ttgataattc aatcaacatt ccgccaacag gttacgtgaa tcagggcgaa
    21661 atgctgactg tcattgtgcc acgcaacatt gatttttctt ctgtatataa ggtccgctga
    21721 tggaaacaat caacatgttt cttgaaagta caggcgtacg cccctttctt caggctgagg
    21781 ggcttacgga aattatcgta aaccgcccgt ttgaaattat taccgaaggc gttaacggct
    21841 gggaatacca tgaagcgcca ggggcttcat acagtgatct gcttgatctc tctattgcaa
    21901 tcaacaaata caacaacagc gcagaacccc ttgatgccct gcaccctatc aagtcgctgg
    21961 ttatgcctgg tggtgagcgt atgcagatta tcatgcctcc ggcctgtgaa gagagatgta
    22021 tctcattcac catacgtaaa ccttccctga ggcgcttcac tctggaggat tacgttagca
    22081 gcaaccggtt cagcaatgtt cgcattgtga atcaggcaaa cctgagcact ctgagtgcct
    22141 cacaacaaaa actgcttgac cttctcgata acaccaggtg tgcccccaga acctggatca
    22201 gttttttaga agctgctgtg gccgccagaa tgaatattct ggttgtgggt ggtacgggat
    22261 caggaaaaac caccattgca aaaaccattg cagacctgtt tccccgtgat cgccgcatta
    22321 tcaccattga agatgtgcac gagatgacgc tgccaaacca tcgtaatcac gtccatctgt
    22381 tctataagca aggcggcgta cagccccgat acctgattga agcggcaatg cgtatgaaac
    22441 cggatcatat ctttctggca gaattacgtg gtgatgaggc atggagttac ctggaagcac
    22501 tgaatacagg acacgaaggc agcgtgacaa ccattcacgc caacagtgcc ttagacgcca
    22561 tttcccgtct tgcgtcgatt gtcaaacagt ccacagtggg gcttaccctg gaccacagct
    22621 tcatactgaa aaccatcaaa acctccatcg atattgtggt gttcttcaga catacccata
    22681 tgacggagat ctactacaac ccgatggaga aaaatcaact actggcagca tgatgttttc
    22741 gtctgcgcca gcagacagaa ggggagaacg aaaactccag ggtagcacag gagccacatc
    22801 gccccgaagc gcggcaacgc cgctactgtt atcgaccgga accaggtcca ccttcccgcc
    22861 ctgccaggcc cgactgactc accgacaacg atgacgctga tttgcgccgc agaatgccgc
    22921 agttagcgct cgcgccagcg agtttacgcc gtccagggtt tccctggacg gggcgctgta
    22981 ctgcgcgtgt gagcgccagc gagctacggg tgcaactctc cggtgaaaaa cctcacgaca
    23041 acggaaaagc tgatgaagaa aaagaaactt gtcctgcctg tacttatagt ctggctgatg
    23101 gccctgacag cctccggctg gctgggaagt tttatactgg tgaaaatcgc ctcactaccg
    23161 actgacaacc tgcaatggag tacgtactta tccacgctgg cctattatgg ctggcaacca
    23221 tccctcaaaa aatacctgtt tgcaggactt gcggggactc tgcttccgtt cctgattgca
    23281 ctcatcctga ctgtcgtgat gtttgtcgcc gttcgggaga aaaaaacggt gcacggtaac
    23341 gccaggctgg caaatgatcg tgacctggca gacagcggtt tttttcccga cgaaaaagaa
    23401 ctgaaaaata agaaataccc gtcactgctc ataggaaaaa tgcctgaaca taaaggacgt
    23461 tttaaatcac gctatctgca atacagcggt cagcagtttc tgatgctgta cgccccgaca
    23521 cgaagcggta agggcgtcgg gatcgttatc cctaacggca tctggtatac cgactcgatg
    23581 gtattcctgg atatcaaact ggaaaacttt cttgagacgg cgggataccg gaaaaatgtg
    23641 ttaaaccagg aagtctatct cttcagccct gacggctacg cacgagaaat cagcggtgaa
    23701 acagtactga caactcaccg ctacaatccc ctttactata ttcgccgtga tcctgtatat
    23761 cgctttggcg acctggaaaa aatcgcggtg atcctgtttc cgatgaccgg aggtgaaaac
    23821 gacacctggg tggaatccag taaaaacgtc tttatcagtc tggttctgtg gttactcgat
    23881 actgaactcg agggcaaatt taaagttaat atgaactcgg tgatgaaact gagtattccg
    23941 gctgatggct caagcctggc aaaatggatg ctgaacgaga tcagaagtcg gggtaatgaa
    24001 gttctcactg aacgctggaa ggcgtaccat gctttaccgg aagaggagaa acagggcaaa
    24061 acgccctcca cctatccgtt aagtgatgac accatccgcc tgttccgcca gttcgcagcc
    24121 aaagaacccg agcaacagga aagcatcatg ctcgacttta accgcaccat gtcaatgttc
    24181 gccaaccctg tcgttgctgc ggctacggaa gataacgatt ttgatatgcg tgatgtgcgc
    24241 cgtaaaaaaa tgacaattta ctacggcatt tctcctggtg cattaaagca atatcagcgt
    24301 ctgactaacc tgtttttttc acaactgctt aatgagaacg tgcgtactct gccagaacat
    24361 gacgcaacac tgaaatatca gtgcacactc atgctggacg agtttacgtc aatgggccgc
    24421 ctcgacgtgg ttcaggtgtc cctggcattc acggcgggct ataacctgcg ttttgtgttt
    24481 attctgcaaa acagggagca gttatttgat gaaaaacatg gttacgggaa aatgggggga
    24541 aacactattc tgaaaaactg tgcggttgaa attttctatc cacctaaaaa agtggatgaa
    24601 agtgtaaaag acgtatcaga aacaatcggg tattatgatt ttgtccgcac tcaaataagc
    24661 cggacaacag ggaaaaaccc tagtacaacc cgcaataaaa ttattgaaaa acgggctgtt
    24721 atgctaccgc aagaaattac tgatttacgg gaccagaaat ttaaagtcaa aggaaagaaa
    24781 acaagttttt ctttaccaca aatcgtaatg agtgagtttt gtcgcccgtt tattgcacat
    24841 aaaattgtca gtttcgatca aaacaatgag tctttttttg ttaacgcaag aaaatattcc
    24901 cgaaaaaata tcatagacac acctgtactt gatattccac aggatgaagt ggttcgtcag
    24961 ttaatgctcc agcgggaaag ggaggaagcg tggaaagcga cgcaacctgc ttttgataaa
    25021 aatgattttg atgatgataa ggaatatttt cctggtgatg gtggtggtga ttaaccatga
    25081 tgccgcttta agcggcatgg aaggcggcac tctgtagctc aatatggcta tgcggcttat
    25141 ccgccgaagc cccgcgccag cgggtactga atccaatatt ctctggtaca gcgctcgcgc
    25201 cccctgctaa gcccaattgc acggaactta cagccactcg gttgtttctg gtcgaagact
    25261 gacaaaatca aagcaattgt tcaaaaagcc tgtctgtggg cttcctcacg gagatataaa
    25321 tatgaaacct cgtttactca tatcagtcac actactttct gcgcttactt tcatactacc
    25381 tgccagtgcc aaaataaaag gactatccgc agccggagat ccttgtgaag tgcttgtctg
    25441 tatggctgaa aaagtacagg gagcaccaca acctggttgt attccggtga atcagagatt
    25501 ctttaacatc cgcgtctaca ctccttttta taatcccggt gcaacctacg gcgcaagaca
    25561 gaattttctt tatacctgtt caggttcggc tgttaaccag gcaaccttaa atatgattat
    25621 gcaacgttat ggcagtcttt tttctggtta acacggataa tgactcattt ttactttttc
    25681 aaagaaaata tttgacgtca ctctgtcaaa actgaaggaa ccataatgag ctttataaaa
    25741 ataaacaggc aacgtgaatt tatggagcga agtaattcac cacgtcttgt actacgaaaa
    25801 gaaaagagaa gaaacggtat tgtaaattgt tatctttcca agacggtaag aggcgacgat
    25861 acattcatta ccatagagtt aaaccctgaa cacaataaaa taagattaaa aacaaccaat
    25921 tcaccagaag gccataaaat gagtcgtggt tgtttttctc ttactaaagt tttcgaatat
    25981 atttttcatg aaaaccttca cgaagacaga attattattg aactggagaa aaaagaaaat
    26041 gggtggtggt atggaaatta ctaaaaacag agaaatccga tttgtaactc ttgcatcaga
    26101 ctttaacaat aaagataaag gaaatagata tgtacctgca tattgtccct aaactttttc
    26161 atcgcatggc gaataaatgc actcttaaat ccatctccat accagaactt gatttaataa
    26221 ttgatggtga atcattgtct gtggggaggc cgtggcctaa taaatgtgta tgggttggaa
    26281 tgcgaaaagg aaggaagtca gttaatggcc tcgttttgca aacagataaa aaattaagat
    26341 ggtttactac tatatacacc tgggatattg aagatatggg gttaatttac catcaagtca
    26401 atacctatat agaagatgat gattttgata tggtttcaca ggaaattctt ttaaatggtt
    26461 cttttgataa gtggagtaac agggttcatt cagcttatga aaataagcct ccagccagaa
    26521 tccagcccaa aatggagtcc ttattaaata aacctggtga aaacagtcat gatgtgtggg
    26581 aagagttcga atggggtgac ttccttctgt ccagagaaga aagcctttta cttcacacta
    26641 ttcagtctga acgattgaat acagattgtt ctttattcaa aagacaacca tctattgaaa
    26701 gtgcattagt tatataaatc agatactctt tggtggttat tatgcaatat gcattagttg
    26761 atgcgctgga acgaaaattt ttgctggatg ctcttgaatt tggtgttctg aaggactgga
    26821 aagaaaatcc ggtaaaagaa cttcctgata ttgatgaatc tgttcacccc ttccatgtct
    26881 gctatggtgg atatttatta aatcctggtg tttcagattc agatattagc agaaaaataa
    26941 aagaccagac aggattctgg ctggcagcta ttgatgatac ccgcatggat tgtcattcaa
    27001 tagcttatta tgatattcac accctccctt tgattttgtg tggacatcaa aagatagttc
    27061 cttttgccgc attaataaaa gctgatgaat gcatcatttc aaaaattgct tcatattctg
    27121 gttttgctgt aacagccttt ttgagaatta aagaccagga tatcgcaacg aatatactta
    27181 accgtgaggg aatttttgcc tttaatggct gtgagcgcag attcagacaa cctgtaagtg
    27241 aagataactg gcaacaggca gtatcagaag aacgcgctat tcgttgtgca aacagattaa
    27301 ttcaatgtaa aggataacaa aatgagactt tttatcgcag aaaaacccgc agtagcaaat
    27361 gatattgtta aggcacttgg tggcaatttt acccgccatg atggctggtt tgaaagtgat
    27421 aacgccattg tgactaactg ttttggtcat attatcgaat cacagccgcc ggaaaactat
    27481 aatcctgaat acaaagcctg gaaggttgaa acgcttcctt tacgtcttta tcccgtgaag
    27541 tatcagcctg ttgaaagtgc agcaaaacag gttaaaacga ttctcgagct tatcagacgt
    27601 ggagacgtga ctgaaattgt tcacgctggc gatcctgatg atgagggaca gctacttgtt
    27661 gatgaagtcc tggaatatgc aggaaacaca aaacccgtaa agcgcgttct gattaacgac
    27721 aacacgcttc cggcagtgaa aaaggcactg gcaaatctta aagataatcg tgatttcaaa
    27781 gggctttacc ttaaggcgct ggcgcgttca gttgctgatg ccgtctatgg cttctccatg
    27841 acgcgtgctt acaccattcc tgcaaaagcc agaggatatc agggcgttct gtctgtcggg
    27901 cgcgtccaga ctcccgttct tggcctgatt gtgaatcgta cccgtgctaa ccagaaccat
    27961 aaatccagtt tttactacac catgaccgga gtctttcagc gtggtgctga tgttatcagg
    28021 gcgaactgga agccagggga atttgctccg ctgacagacc gtaaattact tgataaagcg
    28081 tgggcagacg gaacggcagc atcccttgca ggaaaaccgg ctacagttga agcggcagca
    28141 actgatgata aaaaaacggc tgcaccgttg ccctttaacc tggtacgact ccagcaatac
    28201 atgaacaaaa agttcaaaat gacggcacaa aaaacgctgg atattacgca acaattacgt
    28261 gaaaaatata aagcgattac ttataaccgc tctgattgct catatttatc tgatgaacaa
    28321 ttcagcgaag cgccgcaggt tatcgatgcc ctgaaatcag tctttcctca gtcgctggat
    28381 attgattctt cacgtaaaag caaagcgttt aacagtgcaa aggtgactgc gcatactgct
    28441 ataatcccga ccgccagtgt gcctgatgtt aacgcactca gcaccgacga gcgcaatgtt
    28501 tacctggcga tcgcacaaca ctatcttgtt cagttcatgc ctgaaaaagc ataccaggaa
    28561 gtatcggttg ccattcagtg tggtgatgag tcgttctatg cccgtgccag aaaaacaact
    28621 gacagcggat ttgaggcgtt tcttggcgcg gaaaccacag acgaaggtga atcagaagat
    28681 aatgatgatt ccgcttttga actgctctgt aaaattcgca caggagaaac actgacgaca
    28741 aaagaagtta ttgttaatga gaagaaaaca acaccgccgc cgttattcac cgaagcctcc
    28801 ttgcttgctg cgcttgttcg tgtcgcggat tttgtcactg acccaacgat taaaaaattg
    28861 ttgaaggata aggataaaga caaaaaagat gaacatggtg gcattggtac gccagctacc
    28921 cgcgcagcca ttctggaaac gctgaagaaa agaaactata tcacgctgga aaaagggaaa
    28981 cttattccta ctgataccgg atatgcgctt attgatgccc tgcctggtat agcggttaat
    29041 cctgatatga cagcattatg gtctgaaaag caggctgcca tagaaaatgg cgatctgacg
    29101 gttgaacagt ttattaatga tctgtacggt gaactgacag gcatgatttc tgatgttgac
    29161 ctgggcgaga tgaagattga acccgctgcg ccagcagggc agtttcaacg cctggactct
    29221 ccctgccctt cctgtggtaa acatattgtt atcaggccga aaggttattt ctgtaccgga
    29281 tgtgaattta aaatctggag tgagttttat ggtaagaaaa tcacccaggc acaggccgaa
    29341 aaactggtta aatcagggaa aaccgatttg attaagggat ttaaaaagaa aagtggtgga
    29401 acgtatgata cagttcttgt ccttgaggat aagaaaacag ggaaactggg ttttccggca
    29461 agggctaaga agtgaaaaca aagcaggaat ggctttttca gttaagaaaa tgtacatcaa
    29521 gagatactct tgaaaaagtt attgagatta accgttacaa actgccttta tcagaatcag
    29581 aggcatttta ttctgccgca gatcatcgcc gagcagaact ggtgatgaat aaactttatg
    29641 ataaggtgcc ttccggcgtc tggaagtacg tacactaaat aagaggatta attatatgag
    29701 cgaactgact aaagaagatg aatacggcat tatcagccgt actatgatga atattcgttc
    29761 attgcgtgtt tttgcccgtg aagttgattt tgagcaattg ctcgaaatgc aggaaaaact
    29821 gaacgttgtt gttgaagaac gtcgtgaaga tgctgaacgt gaagcggctg aacgagctga
    29881 gcgtgaacgg aaacgtcagg aactgcttca gttaatcgcc ggagagggtt tctcaccgga
    29941 agaactgctt ggcattaccg aagatgcgcc aaaaacacgt aaaaaaacgt tacccaaagc
    30001 gcctcccaag tatcagtttg aagaaaatgg tgaaactaaa tactggtctg gtcgtggacg
    30061 tgcgccaaaa ccgattgctg aagcactggc tggcggtcgc tcccttgatg agtttctcat
    30121 agagaaatga ttttacagac ctccttttct ctggagggcg ttatcatttg aaatttacgt
    30181 cacgtaactc cgtacatccg tttataatgt tgcttccggc tctactgctg gttgagtgtt
    30241 tagttttggt gataattctc atgtttcgtt tctcagcgtg gggagtacag actttatggt
    30301 gaatgattgg ttgcttttct ctgttagtgt attagttttt ttagtgataa tcctgatgtt
    30361 acatttgtcc ttaaaaacag ccagtcttct ttctgaatgc tctgaatatc gcacgaaatg
    30421 tgataatctt tatcatctat gccagatgca agaggaagag ttagaacatc tacgtgcatt
    30481 cattgatgag cataacatag attattagac gttgccttag gggggtaaat gaaatttttt
    30541 attttaaaga agaataaaga tagcacaatt actaaaaacg acactgtttt ccctgttaac
    30601 aatttctttg tcgttttaac tcttgtgtct tccctggtta actctgccgg aatatttttc
    30661 cccatgatat ctcattctgg taatattatt gagatgtgtt ttttcgtgat gttttcagta
    30721 ttactggctg cattcagttt tgtaagtaca atggggcttg cgaacgcagc agcacccatt
    30781 aaaaatgtgt ttccgctttt gttgttggtt attgtcttaa ttactaatta taatatttac
    30841 ttccgataaa aacggggcgc ggcagcggct tttcttctgc ccgttctccc gtcaattctc
    30901 tccggtaaac ctctccgaaa aatcgcagat ttcacgtagc gcaccgccgt taatgactct
    30961 atcaaaaata ctcaccgata tttttcgcac agcaaacaac ctaactcact gtaaaacaac
    31021 aaatcagctt tgcgccggta acgcatcggg cagctggcca acacgctgat tatcagcgac
    31081 cgcaacaaaa aaattgaaag gaaaaactga tatcattatt aaagggcaga ggtggttaac
    31141 ccctgccctt agttacgttt ccgctgtcat caatcaatga actggcgaat cagttccaga
    31201 accgcaatca gcgcgtttgc aacggcgacg acggctctga gaacgatgat taacaggtta
    31261 atcatccgtc tctccatcaa tggagtagag acaccattgc ttactcatac attgcctgtc
    31321 acaacggctc tcgttatctg ttggatattg agagtcccac tcctgccaga gcactgaata
    31381 cagcaccacc tgtttattca gccagggctt tagattcatt cataccgcag ccagcggagc
    31441 aaaagcagaa atcccaaaca tcacccaaca aacctccgaa gtataaccag ataagcaggc
    31501 ttttgacata gcagtgtatc tgacttacta tacaaacgtt ggatattgag agtcatcctc
    31561 cggtcggagc caccaactca aggccggaaa acaaaaaagc ccgattacca gtcgggcttt
    31621 tttgtttttg attaacggga ccgcgtgtta tccctgtttc tttcccgttc acgctcattt
    31681 ttcaaagcgt tgtcataact cactatttca cgcttcacgg cctctaccgt ttcagggttt
    31741 ttcattgctg ccttgagatc gtttacatcc tcaaaacgta tgaatttgag cgtatctttg
    31801 actgtttgtt tgtcaatatt catttccttc gccagttgct tagaaattcg ctcaatttct
    31861 ctttccatcg ctttttcact attgacagtc cgctttttct tttctgtagt attctcgttt
    31921 ttttcctcga ctgatttagc ttttttctgc ccttgttgtt cctcaaaggc tttaatctgt
    31981 tcattaatcg tctgagcacg agccgaaata ctgttaaccc ctttcgaaag cagatcaacc
    32041 tcttctttcg gcgcaccatt ttctacagct tcattcagta aacgctgggc ttcctggtgc
    32101 tggctggcaa taacttttgc ctcttcctgt agctgctgta gcgatgcgtt attttgctga
    32161 tgaggatttt cagtggctaa cgcggatttg ctttgctcat ctccggtctg tttatcctga
    32221 ccagtagtca cgtctttctc tgttaccggc ttctgttgct gcgcttcttg tggctgctgc
    32281 tcgcgttctt cagaatgagc cgaaacgttc tgctgcacag actcctgttt ttccgcttcc
    32341 tgtaccacgg atgtttcaga cgtggatttt tgagaaacag tttctgactt aacaggctgt
    32401 tccggcactg catcacgcga tttatcatca ttacgtacag attccgctac ttcgggcgtt
    32461 ctttccttag cctgggtgtc tttctcgact gaattaacct gctctgctct cttttcctgc
    32521 tcctgttctg gtttctgtcc ggcgacttgt tgctgacgct ctgcctcgac gctgttttca
    32581 tgtccatgtt cagctttcag ttcctggagg cggacctgca tcatttcatg atatttttca
    32641 ggtcgcagaa taacattcat ccccttctca gctgccgcca ttgcacaggc taccttaaat
    32701 tcttccgagc cattgagttt cagcatccca ccaaatttct cttttgcata aagaagggct
    32761 gcttcagctt tatcctgggt aatttcacca tttacacgaa catgatttcc tttatcagtg
    32821 aaaacagtct gtgttgtctt cggatcaagg taacgcgttt cttttgggct gaccttatga
    32881 ggaacaagat cggttagttc acgttttaca taatcctgaa cttgctggga ttgctgtact
    32941 cgcttaacta cgctgaaaac ttccttttca ttaccggaag aaatgctgtt tccatcaacc
    33001 ggattacgga ttatcttttt taaagaatca cctaaagcca tatcatatct cctgtctgga
    33061 ttccaacggt gatagtatga gtttatttcg cgaaaacatg cctttcgaat tgagtttatt
    33121 tcacgctctc tttccagctt tttaaatata atgtaccctc tagcaacctc ccgttcctgc
    33181 ttgtttctta tcgccagaag ccctctgagt tctgccctgt aattagctat attatcatta
    33241 tgcttatatt taatctcatt atttacccaa cctttatgtt catttaaaca aataaatctt
    33301 tcttttgcct gttgacgcgt aagatttaca cgggtaataa cagcatcaaa aggtttgtta
    33361 tctttctgat cctgccagat atcaagtaat acctcttttg cctcatccca ttcaagattc
    33421 agatgtttag ttaaaaaatc actggcattc atatttcgat tgccaactct gaaacgcgga
    33481 gatccatcct gtccataggt gacagtatgc tttgatggat ctatgttaaa gcgtgtttgc
    33541 agattggaca aataacgttc agcattaatt tctgtgcgaa tttgtttcaa aaaaggtaaa
    33601 tcatcactgc gctgaatcgc cgctttaata tttgattcaa gcgctgactg aactactctt
    33661 cttcctgctc tatcctgcaa ccgtcgcaca tatcgatctg gatcgacttt ttgtcgtcca
    33721 gccgttgata aataaccgta ttcattttgc tgcaataaca actgagattt attaatgctt
    33781 ttcccacctg atcttccgtg aagtccgtaa accagttcgc gtgctggcag acgtggcaat
    33841 ccgattgtct taccggatac aaactttccg gtattgtatt taataccgga ctggtcacta
    33901 ttctgtcgtc ggtcctgtct ttgaaggttg tgtttttcgt cataacgatt aattaccttt
    33961 ttcagataca tttgcttatc ttcaccagca agtgctgcat attctttacg aattttttgc
    34021 cctgccggat gaatatgttt tatctcatga ctgatcgaac tcttccactc tttcagataa
    34081 ctctcaatct gcgtctttgt gggttttaaa agtggtatag ttcgatcaga gatatactga
    34141 cgtgaaaata aagggctttt gagtcgagta aatttagagt ctccttcgag cttcacacca
    34201 aagtagcgat cagacttccc cttattatac tcctttacct caccaaaacg ggaaagataa
    34261 tcagaaaatt gtttctctgt tcggatattc tctgtatcca gcgcatcaaa aaccgtggtt
    34321 ttaaactcac tgtgccgctc acggtaaaaa tcgcctttag ccctggataa tacattagcg
    34381 tggttatggt cactgactct gacaccatct ttaggagaaa caagattgta tttattatta
    34441 atgtgctcct gtatggcatc aagatgtttc aaataatggt taataactcc cccaggattt
    34501 agtttatttc ctgtcaccag attaacttca gggataacaa tgtgaatgtg aggcttacgc
    34561 tcaaccattt ctcctgtttt atgatcaggc atctgtttta tttttggaag atgcgcttcc
    34621 gcataaaaat tatattcatc ttcatgataa gcgttcatca tgagagactt gtattcctgg
    34681 actacatcat gcaactttga ttcagaaaca tcatcttcaa aaaaggataa cgtaatatgc
    34741 atatagcgct cctgaccatt atcagatatc gcgtcaataa ctgattcagt caatgccaga
    34801 tcgccatcca gaactaatcg atggtcaatt tcatctcggg tatattccct tccttgctta
    34861 tgaccttttt caaggtattc gcataccccc ttattgccac cgctgattct gactaacatt
    34921 tattaatccc tgcaacaaac gcatcacgaa tattaacaag aacattaaga tagcgtaaat
    34981 atgtactttc tgaaataaaa ccattgcgat attctgtgtt aatttttttg gctatctgat
    35041 taatattatt gcttgactta ttaaaaagtc tgagtaattc tctgtattca actggacgtt
    35101 tttgtttcaa agtcggaata tcatccagac gattcagtat taccatccga aaaaactcac
    35161 tacgactgac ttcaagagat gtaaaatact catgaaatga ctgatattcc tcatcagtca
    35221 atcgcagtga tatcacatgt tttctctcca cctttgatgt ggaagctaca gccttttttc
    35281 ctttgtcttg ttttttcatg gttcgtagaa cctccatata gcgacctggt tgtagagata
    35341 tactggggta ttagggggag atccccctaa gcacgtcgat tataccggaa tttattccgt
    35401 gtatacatcg ctgtcgtgcc tgtgtcttgt agtaagatca attgaacaag tcacaagccc
    35461 acattacaac ctacacaaca acccacacac aaaagcatga agcccacaaa acaacccaca
    35521 cttaaaccca caaaaaagac cacaagacct attgacaacc cacacaacac cccacataat
    35581 cacccacact aacgaaaagc aaattgattt aaataaagta ctgtgccaat gtaggaggta
    35641 tgagagagga aacataacaa atgaaaaaga tcgatagttt attatctcca atatgcgaga
    35701 cgataaaaga aacaaattca aaatcaaaac aggctgtctt caatatgtat ttatcgaaca
    35761 tacttgaagc aaaaagttat tatacatggg agcaaataac agattacata aataacaaca
    35821 ccaagacaga actagccaaa aagacttata tgaacatgac agagagagcc aaaaaaaggc
    35881 aaaaatgcac tgagcaaaaa gatctaagaa aaagcaccaa tataattgaa aaaaaaacag
    35941 atgaagacaa caaacaaaga gggggcgtaa aaggattttt ttcaacaatc agcgagaaca
    36001 aaaacgaaat agatcacgat ccaagcgcaa ccaatgaaaa atttataaac aaatatcttt
    36061 aagtaaaggt gtatcataaa tgaaaaactc aataaatttt atccttcaag gaaaaggcgg
    36121 tgtaggaaaa tcatttacaa cggcaatact agcacaatac tttattgatg aaaaaaacat
    36181 ggataacgtc gttgtaggcg acacagatcc tgtaaataca actacagtta aagtaaaaag
    36241 attaaatgct gatttaatac aaatcactga aaacagcaaa gtagtccaat ctaaattcga
    36301 tccaatgttc gaatcaatgc taactaatgg tcaaaataca tttgtcattg ataatggtgc
    36361 ttctacattt ttaccattaa ttcagtattt caatgacaac tatgtaatgg atatgtttaa
    36421 cgaagttgaa caagaagttt atatccatac tattattgtt ggcggtcagg cacaagcaga
    36481 tacaatagaa ggttttgaag atcttgttaa actagtaaaa ggaacaaaag taaaaataat
    36541 agtatggatt aatgaatttc agggtgcacc agcacttgaa aacattcctt taattaaaac
    36601 taaattcatt gaaaaaaata aggatgtcat cgctggagtt gtggttattc aggacagaaa
    36661 aagcgatgcc tttgcttctg acatcaaaaa tttaaccgaa aaatcactaa cattaaaaga
    36721 agcccttgaa tcagaatatt ttggattaat ggctaaatct cgtctcaagc gagtttttaa
    36781 cgatatatat gtgcaacttg attcaatcta tgatatgcag cacacagaag cataagtgat
    36841 tatataacag cccttatggg ctgttatatt catgaggaca agtt
//
