LOCUS       NZ_CP103475             2188 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli strain 4973 plasmid pMB9698_7, complete sequence.
ACCESSION   NZ_CP103475
VERSION     NZ_CP103475.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174076
            Assembly: GCF_024918135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:21:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,209
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 68 of 198
            Pseudo Genes (incomplete)         :: 129 of 198
            Pseudo Genes (internal stop)      :: 42 of 198
            Pseudo Genes (multiple problems)  :: 38 of 198
            Pseudo Genes (short protein)      :: 5 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103475.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4973"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB9698_7"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2020-01-09"
                     /collected_by="MDACC"
                     /genotype="ST648"
     gene            join(2102..2188,1..339)
                     /gene="mobA"
                     /locus_tag="M5S50_RS26515"
                     /old_locus_tag="M5S50_26520"
     CDS             join(2102..2188,1..339)
                     /gene="mobA"
                     /locus_tag="M5S50_RS26515"
                     /old_locus_tag="M5S50_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_113451212.1"
                     /translation="MVKNKRSGSETRQRSKVITLRINNKIDREIRRLAKAEGMTISEYI
                     RTSCLNKEIIQRIPSRYLDELLELGRMQKNLFDKGRTPKDKDYLDVMHKIITLCAEAKE
                     VILRNSDIYKEMDLLKQEIKIIKQHYKNKYPGSDLFN"
                     /name="mobA"
     gene            complement(463..876)
                     /locus_tag="M5S50_RS26520"
                     /old_locus_tag="M5S50_26525"
     CDS             complement(463..876)
                     /locus_tag="M5S50_RS26520"
                     /old_locus_tag="M5S50_26525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_113451211.1"
                     /translation="MGAKFLDQKTGVILEEPEFVKLYIRDLCRVKGLSATQYKIFTFML
                     ANMNTDNVVSFGPRTKEKFLSEHSIKPQTFNNNVSQLIDSCLISRIARNEFLVNKKYAV
                     KVDWEKVQKIVWTTTYSNEGVDSTVTIAPKKTD"
                     /name="replication/maintenance protein RepL"
     Typing          1244..1502
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.091"
                     /coverage="100.0"
                     /evalue="6.999999999999999e-64"
ORIGIN
        1 atccgacgcc tggcaaaggc ggaagggatg acgatttcag aatatataag aacgtcatgt
       61 ttaaataaag aaataataca gcgcatccct tccagatatt tggatgaatt attagagctt
      121 ggccggatgc agaagaattt atttgataaa ggaagaacac cgaaggataa ggactacctt
      181 gatgtgatgc acaaaataat cacgctgtgt gcagaagcga aggaggttat tttacgtaat
      241 tctgatatat ataaagagat ggatttatta aaacaggaaa taaaaataat caaacagcac
      301 tataaaaata aatacccagg ttctgattta tttaattgaa acagaacata ggtctttata
      361 aaaatttaaa gacctataat agaaaaatta gcgacatgat tattacatta gttacataaa
      421 tcttactttt gttttatcga catcttttac caatataaga aattaatccg tttttttcgg
      481 ggctatcgtc actgttgaat caaccccttc attactataa gttgttgtcc atactatctt
      541 ttgtactttt tcccaatcaa ctttaaccgc gtatttttta ttaaccaaga attcatttct
      601 agctattcta gaaattaaac atgagtcaat cagttgagac acgttattat taaatgtttg
      661 tggtttaatt gaatgttctg ataaaaattt ttctttagtt cttggtccaa atgaaacaac
      721 attatcagta ttcatattag caagcatgaa cgtaaatatt ttgtattgcg ttgctgatag
      781 tcctttaaca cggcataaat cacggatata cagcttcacg aactccggct cttccaaaat
      841 tacgcccgtt ttttgatcta aaaatttcgc tcccattctc gcctcacagg taatttagtt
      901 accgttttat cggtatctta tgtatgttcc acaactatgt aaatactttt ttttcggtat
      961 tttatcgaca acaacaacag tattccggta tttgaaatac cgctgagcgg tatttcaacc
     1021 tccgctcagc ggtaaaaact ttaccgctga gcggtatctt tattcccgct gagcggtatt
     1081 tagtttggct ctaggccttg ccacacctga ttttcctatc tgtctatgaa tctatcttag
     1141 atctcgtgcg taagtgcact atgccaatac aggttccttt ccgcttcctc gcccactcgc
     1201 gagcctgccg gctcggtcgc tagggctgcg gcgagcggtg ccacctctcg catatcgttg
     1261 acgatccaga tttgatctca ggcgatcgag tcgttggcca cctgttaaac gcgctcagag
     1321 cctctcaaac gcacgaaacg gggtttaaat gcccgtgagg gcatggaagg cgcgaaaaac
     1381 gcttacagag ggtttcagga ggccaggcgc gccgataccc ctaaccaggg gctttcaggt
     1441 cgccaggaac cgcgcaagcc ccctgcaaag cccaaccgca cgggacaggc cgccactcgg
     1501 ttggtgggtg gtcgaagacc gttggtgtta cagcggcggg ggtctggggg tctccccaga
     1561 cggtttccgg tagcgctcaa ggcgctgtcg gaaccgtggc gggttttttt taacaaaaaa
     1621 cgacaggtcg gaaattaaag cgcgaactga acttatattt catatatata aacgcgatag
     1681 ttatcataca cacacatata tattcgcttc atcgttgtaa tctgtgcgat ttctgcctta
     1741 atcgagcatg ttgacggcat cggttaacac taaaaataat actatcgcga ggattaaata
     1801 attatttagc atatatatac cgcagacaat aagagaagaa acgtaatatc aaataaacat
     1861 aataaaaaaa taacaagcac cattttaaaa cctgtgtaaa aaatattgcg tgatgccttg
     1921 cttttttgta ttgaagatac cagcttatga ggcggttttt ttattttccg ccgaaaagtt
     1981 gtagcgaaaa atgggacagg atgtgtattt gtcataccgc gaatacattc gcttgtatga
     2041 aaatacctcc tgtcagggca ggccccgaca ccccgaacgt ggaaaccaga ggttcattgt
     2101 gatggttaaa aataagcgca gcggctcaga aacacggcag agaagcaagg ttataacctt
     2161 gcggataaat aacaagattg acagggag
//
