LOCUS       NZ_CP103415            11192 bp    DNA     circular CON 16-SEP-2023
DEFINITION  Acinetobacter baumannii strain AB2369 plasmid pAB2369-2, complete
            sequence.
ACCESSION   NZ_CP103415
VERSION     NZ_CP103415.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30472217
            Assembly: GCF_024800505.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Zhan,Y., Tan,X., Li,X. and Zhang,Z.
  TITLE     Complete Genome Sequence of Acinetobacter baumannii strain AB2369
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   Zhan,Y., Tan,X., Li,X. and Zhang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2022) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4; FALCON v. 0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 689.66x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/16/2023 04:27:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,714
            Genes (coding)                    :: 3,638
            CDSs (with protein)               :: 3,638
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 21 of 76
            Pseudo Genes (incomplete)         :: 45 of 76
            Pseudo Genes (internal stop)      :: 16 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AB2369"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pAB2369-2"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2022-03"
                     /collected_by="Yongcong Zhan"
     gene            join(11059..11192,1..397)
                     /locus_tag="NX832_RS18995"
                     /old_locus_tag="NX832_18995"
     CDS             join(11059..11192,1..397)
                     /locus_tag="NX832_RS18995"
                     /old_locus_tag="NX832_18995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_259024967.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTF"
                     /name="MobA/MobL family protein"
     gene            427..1218
                     /locus_tag="NX832_RS19000"
                     /old_locus_tag="NX832_19000"
     CDS             427..1218
                     /locus_tag="NX832_RS19000"
                     /old_locus_tag="NX832_19000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259024969.1"
                     /translation="MNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKYNEIHSTLYLP
                     EPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSAENNPFSSLEV
                     MYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDSRRNSTPCFEF
                     EVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGLVFKLSWFYPKFTERVSTSEQTQ
                     LKFLPCFFPDYKLPFAAETEIQSIPEGANRFNLHLGRAKRV"
                     /name="hypothetical protein"
     gene            1631..2566
                     /gene="repM"
                     /locus_tag="NX832_RS19005"
                     /old_locus_tag="NX832_19005"
     CDS             1631..2566
                     /gene="repM"
                     /locus_tag="NX832_RS19005"
                     /old_locus_tag="NX832_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            2644..3162
                     /locus_tag="NX832_RS19010"
                     /old_locus_tag="NX832_19010"
     CDS             2644..3162
                     /locus_tag="NX832_RS19010"
                     /old_locus_tag="NX832_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            3372..3671
                     /locus_tag="NX832_RS19015"
                     /old_locus_tag="NX832_19015"
     CDS             3372..3671
                     /locus_tag="NX832_RS19015"
                     /old_locus_tag="NX832_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            4237..4593
                     /locus_tag="NX832_RS19020"
                     /old_locus_tag="NX832_19020"
     CDS             4237..4593
                     /locus_tag="NX832_RS19020"
                     /old_locus_tag="NX832_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4586..4888
                     /locus_tag="NX832_RS19025"
                     /old_locus_tag="NX832_19025"
     CDS             4586..4888
                     /locus_tag="NX832_RS19025"
                     /old_locus_tag="NX832_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            4881..5090
                     /locus_tag="NX832_RS19030"
                     /old_locus_tag="NX832_19030"
     CDS             4881..5090
                     /locus_tag="NX832_RS19030"
                     /old_locus_tag="NX832_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(5416..5877)
                     /locus_tag="NX832_RS19035"
                     /old_locus_tag="NX832_19035"
     CDS             complement(5416..5877)
                     /locus_tag="NX832_RS19035"
                     /old_locus_tag="NX832_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6182..8593)
                     /gene="znuD2"
                     /locus_tag="NX832_RS19040"
                     /old_locus_tag="NX832_19040"
     CDS             complement(6182..8593)
                     /gene="znuD2"
                     /locus_tag="NX832_RS19040"
                     /old_locus_tag="NX832_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(8721..9035)
                     /locus_tag="NX832_RS19045"
                     /old_locus_tag="NX832_19045"
     CDS             complement(8721..9035)
                     /locus_tag="NX832_RS19045"
                     /old_locus_tag="NX832_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(9022..9309)
                     /locus_tag="NX832_RS19050"
                     /old_locus_tag="NX832_19050"
     CDS             complement(9022..9309)
                     /locus_tag="NX832_RS19050"
                     /old_locus_tag="NX832_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            complement(9577..9876)
                     /locus_tag="NX832_RS19055"
                     /old_locus_tag="NX832_19055"
     CDS             complement(9577..9876)
                     /locus_tag="NX832_RS19055"
                     /old_locus_tag="NX832_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(9993..10754)
                     /locus_tag="NX832_RS19060"
                     /old_locus_tag="NX832_19060"
     CDS             complement(9993..10754)
                     /locus_tag="NX832_RS19060"
                     /old_locus_tag="NX832_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     Typing          1632..2566
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..394
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          392..1032
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 tggtcgatgt atgcctgcct ttgtaaaaga tcccattgat ttttggcagg cagcagatac
       61 gtatgaacga gccaatgcca aggcctacat ggagtatgaa attgccctac cgaatgagtt
      121 cacgtcagaa cagcgtaaaa cgctcataga gacgttttta gacaaacata tagttccaca
      181 gcaatatccg cactcatacg ccatacacaa cgtaaaatca cgtatcagtg gcgaagatca
      241 gcctcattgt catctgatgt tctcgctaaa agcggatgac ggcattgaac gcagcgcaga
      301 acagtacttc aaacgctata accctcaaga tccagccaaa ggcggtgcca aaaaaatcca
      361 acttcaagat ggccatgaag actattccac tttttgatct atatccgaaa acaatgggaa
      421 aatcatttga atgatgccct tgcacagcac tgcccgaccg tcacctacac actggatggc
      481 caagacatca ccatcaaaaa tcaggtttca gcagacagct atgaaaaata taacgagatc
      541 cacagcacgc tataccttcc agagccaaaa ctaggcgttg gacaacaaaa tgcgacagcg
      601 gaatatctcg cccaaatcca aaaaatcaga gaacacaacc agcaagaacg agaactcgaa
      661 gaacgccaaa agcaattaga tcaacaacat gaatattttt atagtgctga aaacaaccca
      721 ttttcatcat tggaagtcat gtattacctg aattacttaa tcaagaatgc aacaaataag
      781 gacaaactaa aaaatgattt gaatgaaatt atcaaaaacc aaggccctat acttaaacaa
      841 gaagcttggt ccatctacga aatgaatgaa aaactagaag actcaagacg taacagtacc
      901 ccatgttttg agttcgaggt gcattcactt cctgaatacg tattaccgat acaaaatacg
      961 cctcccaaac gcactcaatc agaaccagta caacctaaag ctgatgatta tgattacggt
     1021 ggcctggttt ttaagctcag ttggttttat ccaaagttta cagagcgagt gtctacgagc
     1081 gaacagacac aattaaaatt tttgccatgc tttttccctg attacaaact cccctttgct
     1141 gctgagactg agatacagag catcccggag ggtgcgaaca gatttaattt gcatctaggt
     1201 cgagcgaagc gagtataaaa aacttatgag cgaagcgaat tccgagttgc ttttgctttt
     1261 ttgaagttat cacgagatta acccaaaaat tgccccttcg aactgagcgt aagcgaagtt
     1321 caatagagtt tgagcgtagc gaaaacatag ggcaattttt cattcattgg gcttttaatt
     1381 atttttaatc ttttaaatgc ttttaaataa gctgtaaacc tttatttata atagtttcat
     1441 agttaataaa actaggttta tcgacccata aaactaggtt tatcgaccca taaaactagg
     1501 tttatcgacc cataaaacta ggtttatcga ccttaaagct aggtttatcg acccataatt
     1561 aagttaattt acaactcaat tgaaatagtc tattgtacaa cctagtttaa ttataaattt
     1621 attattagaa atgagtgaat taatcgtaaa ggataatgct ctaattcaag caagttatac
     1681 cttagataca gttgaacaaa gactgatcct attagccatt gctgaagctc gagaaactgg
     1741 acatgggata actgaaaata gtcttttaga agtacatgca agtagttata taaatacttt
     1801 taatgtcgag aaacataccg cttataccgt actcagagag gcatctaaaa gcttatttga
     1861 tcgctatgtc acataccatg acattaatcc taagacaggt aaggatcgta gctttcattg
     1921 tcgttgggtc gacaaaattg ggtatgaatc tcaatcagga attattttcc tacgattcac
     1981 ccaagatatc gttccactca taactcgact tgaagaaaat ttcactaaat atgaattgca
     2041 gcaggtttct aggttaagta gctcatatgc tattcggcta tacgagctat taattcaatg
     2101 gagatctgcc ggaaaaacgc cactttttga tctatctatc tttagacaac aacttggtgt
     2161 caaacctcat caatacaaaa caatgagtaa ctttaaaaca tatgttttag attttgctct
     2221 taagcaggta aatgagttaa ccgatataac agctaaatat gagcaacata aaaaagggcg
     2281 ttctatttca ggtttttcat tcactttcaa acagaaaaaa atgagtaatc tgccaataaa
     2341 aaataagcgt gacccagaca ctatagatat tttctcaaaa atgacagatg ctcaacgcca
     2401 tctgttttcc cacaaactgt cagaacttcc tgaaatggga aagtattctc atggtacaga
     2461 aagctatccg caattcgctg tacgcattgc agaaatgctt caaaacccag aaaagtttaa
     2521 agaactctat ccttatctcc aaaaagtagg cttcaaagct gcatagaaga tagacttgct
     2581 gatttgtcta ttagacagtc taatagacac taatttttta caagattagg cagagattta
     2641 cacatgaaaa gccttacagt tcttgagctt tctaagcttt acaatattaa tcgacaaacc
     2701 atttacaaca atataaagaa aggtatttta agtaaaaatt ctgacaataa gattgatctt
     2761 gcagaagcga tccgggtatt tggtgagcca gtacaaaaac agagtgtaaa agaaactgta
     2821 aaagtagaca gtccaacttc ggcagaagtt ttactcctta gacagcagat agacctacta
     2881 aaaaatcagc tagatgatgc aaaaaataga gaaagcttct atcaaaatca aattgaaact
     2941 atgcaacgcc tattggaagc tccaaaaacc aatacaacac aatctgatga tcctggatca
     3001 acacaggcag aatccacagt agaagaaaaa gaggacgtca tagctatgcc tcctaaggag
     3061 tcagaggtta tcgagaataa gcgtattcca gtccctgaac atattgagcc agaaccagaa
     3121 aaaaggggct tctggagccg ttttttcaag ccctatggat gataggacta tttaatcaga
     3181 ctaatcaaaa ccgtggaaca atgatcaata attgatcgtg aaacataaaa aatcactaaa
     3241 acagcctgag cgtgactgct taggctgttt ttacatccat aagacattgt tttaaaatgc
     3301 tttttacaaa gaatttcgta taaggtgtat tatgttaatt ttagggaaac ttaattctat
     3361 aggtgatacc aatggacaaa aagatagaag agaaaactaa agatattaat gaaaaaattc
     3421 aaaaattgga tttagctatt caagaactaa tagaaactac agacaatttt tttattaatg
     3481 ataagtacta tggaattgaa tgtgctttta ttttagacca tgcactaaat attaaaatgc
     3541 actttttcaa caagtatcca gcatttgcac cacctcactt acgaggagta gaaaattatg
     3601 atcgaattat acatattaga gaaaataatc ctgaagataa gctagaacaa atttctcctc
     3661 ttaaagacta aattttctaa tcttgtactt agagtcccct actatttaac ataatggata
     3721 ttatgcgaaa tggatcttta agattacttt aagatcagta gcttacattg agtcataccc
     3781 tctggaacga cctcgttcaa ccgacttaga aacaagtcgg tttttttatg ctcaattttg
     3841 atacttcttg ccaataaaat tgtttaaaaa ataatcaaaa atagcaattc catagagttt
     3901 ataagctttt ttacccaaaa gaatccacta tttctgtgga taaccactgg gataaatttt
     3961 gattattttt tgtacgtttt ggcttacagc gtttcacgaa attgtctgta tctatccccc
     4021 gatgaagctc ttatgatcga tctatatgga caggatgttc aatgacttaa aacaacaata
     4081 ataatcagtc aggcaccatg accatcaggt agtcagccct acctatgcgc tccataccga
     4141 aaaatcccga catgacgtcg ggatttttta ttgccatttg caaagtcatt caatcagatt
     4201 taatatatag actgtaatct atattgtgat tgtaatatgt ggacagtcat tacaacggat
     4261 ctgtttaatg aatggcttga acagcaagat gaagcaacac aagagaaagt cttagctgca
     4321 ttggttgttc tacaacagca agggcctagt ttaggtcgtc ctttagtaga taccgtctac
     4381 gattctaaat ttaccaatat gaaggaacta cgtgttcagc atcgtggtaa accgttaagg
     4441 gcattttttg cattcgatcc cctaagacaa gcgattgtgc tgtgtattgg tgataagagt
     4501 aataaaaagc gtttttatac agagatgcta gcaattgcag atgagcaata cgcacttcat
     4561 ctctcaactt taggagatca atctaatggc taagagctta caagaattat tggctagtcg
     4621 ctctccagag agtcaagctc gtattcaaaa aatggctgat gaattacttt tagaaagtca
     4681 gcttcacctt atccgagaag agttagaaat ttcccagaaa gaactagctg caacccttgg
     4741 aattaaacag ccgtcattat cagcaatcga aaatcgtggt catgatctta aaatttcaac
     4801 gatgaaaaag tatgttgaag caatgggtgg aaaacttcgt attgatgtag agcttccaac
     4861 aggaaaacac atagggttca atgtctaata ataaaccttt gaaaaatagt actaaattac
     4921 ttgttaactt agacaagttt atatttttag tcaatgctgc cgatagccta gaagaaattg
     4981 aaattattag agatttatgc tgtgaatatt tttcacattg taagaggcca agctattaca
     5041 ttgatatttt tgacaatgct tattggataa aatattacga aatagcttaa tccttctaga
     5101 ttcgcataag agattttatg ttaaatgccg aaatgaatta aaaatagaaa tgtagttgag
     5161 ctgatgcttg ctcatttctt tatttttagt aattgaggtg aacatactgt cagtacatgg
     5221 tagttataag taacgtgtaa ctttgtttgc acaaagattt gagttatgcc aagtgatgga
     5281 ttgatttgta cttaaaattt taagcttatc cacttgcttt gcaagccact acactttatt
     5341 tacaatccta tgttgtggaa gtaagggatc ttgtactcgc ccagtatgtt caacttgatt
     5401 atatctaaag gactattagg cttcaatcaa atcaattttc caattagtcg actgaattaa
     5461 cacgtttaaa tcacgtaatt taagactaat atcatcagct tgtttttgta atgatgaaac
     5521 tggaataact ttatgccact ttatttcccg cgtactataa cgatcagatt cacgagatgt
     5581 attacttatt gctgcagtta gggttttgtg ctgttctaca aattcatcac gcaaagtcaa
     5641 taaagcaagt agagaacggc catcatttaa ttttgttaaa gcattagttt catgaattgc
     5701 ataacttaat ttttgaaatt cttgaattaa agaaaatact tgttttagta gttcatttgg
     5761 atcttcagat ggttgatcac catcttgtac caatacattt gcattgatac gttgttttag
     5821 tgaaataatt tttttttgtt gatcacttcg aagtaagaga gcttctgcta atttcatttt
     5881 tcacctatta accaattaga attaagtttt aaacagctta tatcctcatt ctttaaagat
     5941 caaaaaaatt gctttttatc tagctaaaag gtgtctacaa aatcggtggc tattcagtgc
     6001 tgaaaaggct gccgacacat aaagtgaccc agattgaaga gttactgcca cactgctgga
     6061 aacctaaatc gaattaaaaa ataggtatgg gattcagcgg acgcttacct taattattaa
     6121 tctaatcatt ttataagaaa aatcttctaa gaaattttct tagaagatta cagtatctta
     6181 tctagaaatt aagatttaag cctaaactaa aatttcttcc aatttgggga atatatggca
     6241 aaaatgtttc atgggcatag actttctgat caagtaaatt attcgcttta aagaataaat
     6301 catattctgt gtgagatagc ttatttttat atgctaaagt catattaagc atattataac
     6361 ctgatgtaac ctgctcaaat ttagaaatat tatcttgttt aaagactcga taatattcta
     6421 tttcaccttt aagcgattca tcaaaatcag cttttagtcg tgtacccaaa cgagcaggag
     6481 gtaatctagg agtgtaacga tccttttggg gagctaaagt cggttctcta ttccagatat
     6541 cgtatgcaat tacagcatca ggtaaatttg ttaaacggcc tttcacataa tctccaaaaa
     6601 gagagccgtg ataaacagaa ttaaattgat accctatgtt gccctctaat ccataaaaat
     6661 tagcagcaga ctggctataa tgattaatac gtagtgtatg ctgatctcta acttttgtag
     6721 ttcctaaaac ttcattgagt gtctgtaaat atatatagtt atcaaaatca taatagtaag
     6781 tactgatttg ataatcaagt aaatcatcct tataggccag actgatttct aaattattgg
     6841 atttctcttt tcttagaaag cggttaccta tttcaaaaga attagtagcg agatgcatac
     6901 catgagtata catttcttgt gcattaggta atctttcttg atgtgtacct gttaatgata
     6961 agatataatt tggagcaaat ctccagtgga ctgtacctgc ataagaaaaa gctgtttctt
     7021 tgttaggttg aacagcttct aaaatagatt ttaaattttg agccctgatt ttattatttt
     7081 tttctacata aggactgtta tatttattag gccaaggttt cattgaatct ttaatttttt
     7141 ctatatcata atccatacta actttctgct tttctatacg agcacctagt tcgaaagtaa
     7201 agtcgttcca gttatatttt tctaggccaa atacactaaa gtttttagtg acatttcgat
     7261 ttaatagctg ctgattatct agataggtgg tatggccttc ttgagaatgg actggtgata
     7321 aagcactatt atcctgttcc aaatattgta gccccaagat accggttaat tcccctaatg
     7381 gctgatgtga taactctatg cggcctactt ttcctgtatt tttaaaaaag ttagtaatct
     7441 tatctccctc gagttcattg tgaaaataat ctatataact caatgaggta cgaattttgt
     7501 caataccggt aaatggttga gtgaattctc cacgcacatc ataacgtcga gtatttaaat
     7561 caatataagg agcattatga gagaaattag tttcatgaat atgattatgc tgatgacaat
     7621 caggacgtgg atttatgtaa ttaatatctt gctcatccat caattgagga taatataaaa
     7681 gataattttt taatccacta attcgtgaat cgataccgat tgcagaggca ccacaacctt
     7741 catataaatg gctatgggct ggtaaaccat attcatcatg acgatgtgta taagaaactc
     7801 ctaaataagt attatcgtct atccaggata atccaagcgt acctactctc gactctgccc
     7861 agctttctgg taaatatttt aaatttttat aaatactgtt ttggttttca agatcttcta
     7921 aagaattata agagctagac tggtaatgag gagttttata gttaccagca ttacgatata
     7981 acccttctgc ccgtaaagct acatgtgggc ttaagccaac tgttacacct gctgtggtca
     8041 acttctcatt actgccagta ttaaaacgta atgtagtatt accctctaat ccgtcttgag
     8101 gcatttgggt tggaatttta tagtcaatga cattgactac ccctgccgca ctatttgaac
     8161 tatacaaaag ggtacttgca ccacgtataa tttcaataga cttagctaaa cttggttcaa
     8221 ctgtgacagc gtgatctgga gacatattag acatatccaa cacgtcagca ttattctgta
     8281 aaactttaat acgttttcct tcctgcccac gaataatagg agtagatgct ccacctccat
     8341 attgattaga atgaattcct aattccgtac ccaaggcatc ccctagagtt gtcgaacgct
     8401 gtttaagttt actagtatca ataaactgat tactaacatt tagacttttg cctaacagct
     8461 cagaccctaa ctcatcagat tgtgcagtta atactattgt atttaaggta gtaacaggcc
     8521 cttcagctgc caataaaatt ttcgaggccc ctaaagtaat taaagctaaa aatagtttac
     8581 ttttatttag cataaaacaa ccaaatttaa tgttatatta taatataaca cttaaaaatt
     8641 tataaaatct actatttaaa tttttgaatg gtaatccccc ctaaaaatga agtcaaaaat
     8701 gggagcctaa gctcccattc ttattgatga ttttttaatt cttttagcat agcatctcgt
     8761 aaaatttcat tcatacgtgt ttgataacct ttgccttgtg ctttgaacca agcaagtaca
     8821 tcagcatcta accgaattga agtttgttgc tttactgggc ggtaaaattg attttggcgt
     8881 acagcattgc tccagtctgt aatttcagga atatctgata gatctagctg atcatcagga
     8941 accgtgcctt tagcaagcaa gcgttgaatt tcagcatctt gcttctcacc aaatttctca
     9001 ttcagctctt tgtgtgagta tctaaccatg ctcatatttg tttcgctccg ctttagtgac
     9061 ttgccttgca ctaatgattc ggatgatttc acagtcatct tcatcaaaaa tggtgtgagc
     9121 caccaataac attagtacgc ctttaactcg tccaatggtt tgccaacgtt cttcaccatt
     9181 agtatatctg tcttggattg agatccgtaa tggatcttca aaaacaaggc ttgcagtttc
     9241 gaaagagata tcatgctttt tctgattctt tcgattctta gcctcatccc attcaaaata
     9301 ctgttccata aaaaatattc aaatttgtat atacaataat gtatcacgaa attgtatcac
     9361 tcaagttgag aagcgtaatt tttagtcaga tttattggca aaaactatca aaatcgatca
     9421 taaaaaagcc gacttgtttc aaagtcggct ttttgggcat ttttggattt ttatattttt
     9481 attaagtagt tgatatttaa attaatatac aagtgtactt cgtataatat ccattatgtt
     9541 aaatagtagg ggactctaag tacaagatta gaaaatttag tctttaagag gagaaatttg
     9601 ttctagctta tcttcaggat tattttctct aatatgtata attcgatcat aattttctac
     9661 tcctcgtaag tgaggtggtg caaatgctgg atacttgttg aaaaagtgca ttttaatatt
     9721 tagtgcatgg tctaaaataa aagcacattc aattccatag tacttatcat taataaaaaa
     9781 attgtctgta gtttctatta gttcttgaat agctaaatcc aatttttgaa ttttttcatt
     9841 aatatcttta gttttctctt ctatcttttt gtccattggt atcacctata gaattaagtt
     9901 tccctaaaat taacataata caccttatgc gaaattccaa tgtaagccca aaatagaaat
     9961 gtccaacttc tctaaaatcc ctgctaaata aactactctt cgcctttttc taaggccttt
    10021 ttaatttcaa ttactgacaa taattctccg cccttttgcc aggtatgtaa tccttctaac
    10081 acctgaataa aaagtttatt tgctttaaaa tattcatttt catatgtctt aacatcttta
    10141 aaaattaaag atttacgtac ttcattatga aactttgtcg tattcacaat ccgattggta
    10201 atctgttgag gagtatcatt gctaatatca atattgagtt tcttaaatgc gacaagaaca
    10261 gcaccaccta attcaaattt acgttgattt tcagctttgc gttttttccc agcatccttc
    10321 ttctgtagct gactcaaata caattctaaa tcactgtatt caagcttttg cgctttaaaa
    10381 ttcagatacc gtgtcgccaa ggtttttaaa atatttagtt ccatctgatc tagtgcattc
    10441 ttttgaaaaa gttcatacag atctaaaaac ttctgttcat actcggtttt aagaggcaga
    10501 ttattcaact cttcaaacag caatttcaac ttcagatgtt cacgataagg ttttaaagcc
    10561 tctttgacca gaacctgatc tgtctgagtt agcccctcaa aattacaatc aacactattt
    10621 aaagccttta acacttgaga ttgaagatca gaaagctgtt cttgctgttc taaaaactct
    10681 acaaatgcat gtaactcagc aatctgtaac aactgagcag gtcgaccacg cttttttgct
    10741 ttttccattt ccatcatctg ccgccaaatt ttcagaactg caacctcttt aaatatggtt
    10801 ttaccatatt taaagagggc agtctgaaaa taattattta atataaaaat aaaaattctt
    10861 ttcaagaaat tttattttta tattaaataa ttcgcacttg acagtttctc ctcatcggag
    10921 aaactagtac gcaatggggc gcttccccct tgaccccctt caaccggatt tttcagccat
    10981 acaggcattt caaatccggg cggttttcat tccgtttcac tgcataaaaa ccgaacccac
    11041 aacccagcag aggtgaacat ggccagctac cacttcagcg tcaaaagcaa aaataagggc
    11101 tatgcgaccg gccattatct ctacatttcc cggctgatgc agtatgaagg catccgcaaa
    11161 accagcaatg agaccataga acatattgaa cc
//
