LOCUS       NZ_CP103414            11193 bp    DNA     circular CON 16-SEP-2023
DEFINITION  Acinetobacter baumannii strain AB2369 plasmid pAB2369-1, complete
            sequence.
ACCESSION   NZ_CP103414
VERSION     NZ_CP103414.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30472217
            Assembly: GCF_024800505.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Zhan,Y., Tan,X., Li,X. and Zhang,Z.
  TITLE     Complete Genome Sequence of Acinetobacter baumannii strain AB2369
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11193)
  AUTHORS   Zhan,Y., Tan,X., Li,X. and Zhang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2022) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4; FALCON v. 0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 689.66x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/16/2023 04:27:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,714
            Genes (coding)                    :: 3,638
            CDSs (with protein)               :: 3,638
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 21 of 76
            Pseudo Genes (incomplete)         :: 45 of 76
            Pseudo Genes (internal stop)      :: 16 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103414.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AB2369"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pAB2369-1"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2022-03"
                     /collected_by="Yongcong Zhan"
     gene            166..452
                     /locus_tag="NX832_RS18930"
                     /old_locus_tag="NX832_18930"
                     /pseudo=""
     CDS             166..452
                     /locus_tag="NX832_RS18930"
                     /old_locus_tag="NX832_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /name="BrnT family toxin"
     gene            439..753
                     /locus_tag="NX832_RS18935"
                     /old_locus_tag="NX832_18935"
     CDS             439..753
                     /locus_tag="NX832_RS18935"
                     /old_locus_tag="NX832_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            881..3292
                     /gene="znuD2"
                     /locus_tag="NX832_RS18940"
                     /old_locus_tag="NX832_18940"
     CDS             881..3292
                     /gene="znuD2"
                     /locus_tag="NX832_RS18940"
                     /old_locus_tag="NX832_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            3597..4058
                     /locus_tag="NX832_RS18945"
                     /old_locus_tag="NX832_18945"
     CDS             3597..4058
                     /locus_tag="NX832_RS18945"
                     /old_locus_tag="NX832_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(4384..4593)
                     /locus_tag="NX832_RS18950"
                     /old_locus_tag="NX832_18950"
     CDS             complement(4384..4593)
                     /locus_tag="NX832_RS18950"
                     /old_locus_tag="NX832_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(4586..4888)
                     /locus_tag="NX832_RS18955"
                     /old_locus_tag="NX832_18955"
     CDS             complement(4586..4888)
                     /locus_tag="NX832_RS18955"
                     /old_locus_tag="NX832_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4881..5237)
                     /locus_tag="NX832_RS18960"
                     /old_locus_tag="NX832_18960"
     CDS             complement(4881..5237)
                     /locus_tag="NX832_RS18960"
                     /old_locus_tag="NX832_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5803..6102)
                     /locus_tag="NX832_RS18965"
                     /old_locus_tag="NX832_18965"
     CDS             complement(5803..6102)
                     /locus_tag="NX832_RS18965"
                     /old_locus_tag="NX832_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(6219..6980)
                     /locus_tag="NX832_RS18970"
                     /old_locus_tag="NX832_18970"
     CDS             complement(6219..6980)
                     /locus_tag="NX832_RS18970"
                     /old_locus_tag="NX832_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            7285..8454
                     /locus_tag="NX832_RS18975"
                     /old_locus_tag="NX832_18975"
     CDS             7285..8454
                     /locus_tag="NX832_RS18975"
                     /old_locus_tag="NX832_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            9051..9986
                     /gene="repM"
                     /locus_tag="NX832_RS18980"
                     /old_locus_tag="NX832_18980"
     CDS             9051..9986
                     /gene="repM"
                     /locus_tag="NX832_RS18980"
                     /old_locus_tag="NX832_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            10064..10582
                     /locus_tag="NX832_RS18985"
                     /old_locus_tag="NX832_18985"
     CDS             10064..10582
                     /locus_tag="NX832_RS18985"
                     /old_locus_tag="NX832_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            10792..11091
                     /locus_tag="NX832_RS18990"
                     /old_locus_tag="NX832_18990"
     CDS             10792..11091
                     /locus_tag="NX832_RS18990"
                     /old_locus_tag="NX832_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     Typing          9052..9986
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7364..8451
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataaaaatcc aaaaatgccc aaaaagccga ctttgaaaca agtcggcttt tttatgatcg
       61 attttgatag tttttgccaa taaatctgac taaaaattac gcttctcaac ttgagtgata
      121 caatttcgtg atacattatt gtatatacaa atttgaatat tttttatgga acagtatttt
      181 gaatgggatg aggctaagaa tcgaaagaat cagaaaaagc atgatatctc tttcgaaact
      241 gcaagccttg tttttgaaga tccattacgg atctcaatcc aagacagata tactaatggt
      301 gaagaacgtt gcaaaccatt ggacgagtta aaggcgtact aatgttattg gtggctcaca
      361 ccatttttga tgaagatgac tgtgaaatca tccgaatcat tagtgcaagg caagtcacta
      421 aagcggagcg aaacaaatat gagcatggtt agatactcac acaaagagct gaatgagaaa
      481 tttggtgaga agcaagatgc tgaaattcaa cgcttgcttg ctaaaggcac ggttcctgat
      541 gatcagctag atctatcaga tattcctgaa attacagact ggagcaatgc tgtacgccaa
      601 aatcaatttt accgcccagt aaagcaacaa acttcaattc ggttagatgc tgatgtactt
      661 gcttggttca aagcacaagg caaaggttat caaacacgta tgaatgaaat tttacgagat
      721 gctatgctaa aagaattaaa aaatcatcaa taagaatggg agcttaggct cccatttttg
      781 acttcatttt taggggggat taccattcaa aaatttaaat agtagatttt ataaattttt
      841 aagtgttata ttataatata acattaaatt tggttgtttt atgctaaata aaagtaaact
      901 atttttagct ttaattactt taggggcctc gaaaatttta ttggcagctg aagggcctgt
      961 tactacctta aatacaatag tattaactgc acaatctgat gagttagggt ctgagctgtt
     1021 aggcaaaagt ctaaatgtta gtaatcagtt tattgatact agtaaactta aacagcgttc
     1081 gacaactcta ggggatgcct tgggtacgga attaggaatt cattctaatc aatatggagg
     1141 tggagcatct actcctatta ttcgtgggca ggaaggaaaa cgtattaaag ttttacagaa
     1201 taatgctgac gtgttggata tgtctaatat gtctccagat cacgctgtca cagttgaacc
     1261 aagtttagct aagtctattg aaattatacg tggtgcaagt acccttttgt atagttcaaa
     1321 tagtgcggca ggggtagtca atgtcattga ctataaaatt ccaacccaaa tgcctcaaga
     1381 cggattagag ggtaatacta cattacgttt taatactggc agtaatgaga agttgaccac
     1441 agcaggtgta acagttggct taagcccaca tgtagcttta cgggcagaag ggttatatcg
     1501 taatgctggt aactataaaa ctcctcatta ccagtctagc tcttataatt ctttagaaga
     1561 tcttgaaaac caaaacagta tttataaaaa tttaaaatat ttaccagaaa gctgggcaga
     1621 gtcgagagta ggtacgcttg gattatcctg gatagacgat aatacttatt taggagtttc
     1681 ttatacacat cgtcatgatg aatatggttt accagcccat agccatttat atgaaggttg
     1741 tggtgcctct gcaatcggta tcgattcacg aattagtgga ttaaaaaatt atcttttata
     1801 ttatcctcaa ttgatggatg agcaagatat taattacata aatccacgtc ctgattgtca
     1861 tcagcataat catattcatg aaactaattt ctctcataat gctccttata ttgatttaaa
     1921 tactcgacgt tatgatgtgc gtggagaatt cactcaacca tttaccggta ttgacaaaat
     1981 tcgtacctca ttgagttata tagattattt tcacaatgaa ctcgagggag ataagattac
     2041 taactttttt aaaaatacag gaaaagtagg ccgcatagag ttatcacatc agccattagg
     2101 ggaattaacc ggtatcttgg ggctacaata tttggaacag gataatagtg ctttatcacc
     2161 agtccattct caagaaggcc ataccaccta tctagataat cagcagctat taaatcgaaa
     2221 tgtcactaaa aactttagtg tatttggcct agaaaaatat aactggaacg actttacttt
     2281 cgaactaggt gctcgtatag aaaagcagaa agttagtatg gattatgata tagaaaaaat
     2341 taaagattca atgaaacctt ggcctaataa atataacagt ccttatgtag aaaaaaataa
     2401 taaaatcagg gctcaaaatt taaaatctat tttagaagct gttcaaccta acaaagaaac
     2461 agctttttct tatgcaggta cagtccactg gagatttgct ccaaattata tcttatcatt
     2521 aacaggtaca catcaagaaa gattacctaa tgcacaagaa atgtatactc atggtatgca
     2581 tctcgctact aattcttttg aaataggtaa ccgctttcta agaaaagaga aatccaataa
     2641 tttagaaatc agtctggcct ataaggatga tttacttgat tatcaaatca gtacttacta
     2701 ttatgatttt gataactata tatatttaca gacactcaat gaagttttag gaactacaaa
     2761 agttagagat cagcatacac tacgtattaa tcattatagc cagtctgctg ctaattttta
     2821 tggattagag ggcaacatag ggtatcaatt taattctgtt tatcacggct ctctttttgg
     2881 agattatgtg aaaggccgtt taacaaattt acctgatgct gtaattgcat acgatatctg
     2941 gaatagagaa ccgactttag ctccccaaaa ggatcgttac actcctagat tacctcctgc
     3001 tcgtttgggt acacgactaa aagctgattt tgatgaatcg cttaaaggtg aaatagaata
     3061 ttatcgagtc tttaaacaag ataatatttc taaatttgag caggttacat caggttataa
     3121 tatgcttaat atgactttag catataaaaa taagctatct cacacagaat atgatttatt
     3181 ctttaaagcg aataatttac ttgatcagaa agtctatgcc catgaaacat ttttgccata
     3241 tattccccaa attggaagaa attttagttt aggcttaaat cttaatttct agataagata
     3301 ctgtaatctt ctaagaaaat ttcttagaag atttttctta taaaatgatt agattaataa
     3361 ttaaggtaag cgtccgctga atcccatacc tattttttaa ttcgatttag gtttccagca
     3421 gtgtggcagt aactcttcaa tctgggtcac tttatgtgtc ggcagccttt tcagcactga
     3481 atagccaccg attttgtaga caccttttag ctagataaaa agcaattttt ttgatcttta
     3541 aagaatgagg atataagctg tttaaaactt aattctaatt ggttaatagg tgaaaaatga
     3601 aattagcaga agctctctta cttcgaagtg atcaacaaaa aaaaattatt tcactaaaac
     3661 aacgtatcaa tgcaaatgta ttggtacaag atggtgatca accatctgaa gatccaaatg
     3721 aactactaaa acaagtattt tctttaattc aagaatttca aaaattaagt tatgcaattc
     3781 atgaaactaa tgctttaaca aaattaaatg atggccgttc tctacttgct ttattgactt
     3841 tgcgtgatga atttgtagaa cagcacaaaa ccctaactgc agcaataagt aatacatctc
     3901 gtgaatctga tcgttatagt acgcgggaaa taaagtggca taaagttatt ccagtttcat
     3961 cattacaaaa acaagctgat gatattagtc ttaaattacg tgatttaaac gtgttaattc
     4021 agtcgactaa ttggaaaatt gatttgattg aagcctaata gtcctttaga tataatcaag
     4081 ttgaacatac tgggcgagta caagatccct tacttccaca acataggatt gtaaataaag
     4141 tgtagtggct tgcaaagcaa gtggataagc ttaaaatttt aagtacaaat caatccatca
     4201 cttggcataa ctcaaatctt tgtgcaaaca aagttacacg ttacttataa ctaccatgta
     4261 ctgacagtat gttcacctca attactaaaa ataaagaaat gagcaagcat cagctcaact
     4321 acatttctat ttttaattca tttcggcatt taacataaaa tctcttatgc gaatctagaa
     4381 ggattaagct atttcgtaat attttatcca ataagcattg tcaaaaatat caatgtaata
     4441 gcttggcctc ttacaatgtg aaaaatattc acagcataaa tctctaataa tttcaatttc
     4501 ttctaggcta tcggcagcat tgactaaaaa tataaacttg tctaagttaa caagtaattt
     4561 agtactattt ttcaaaggtt tattattaga cattgaaccc tatgtgtttt cctgttggaa
     4621 gctctacatc aatacgaagt tttccaccca ttgcttcaac atactttttc atcgttgaaa
     4681 ttttaagatc atgaccacga ttttcgattg ctgataatga cggctgttta attccaaggg
     4741 ttgcagctag ttctttctgg gaaatttcta actcttctcg gataaggtga agctgacttt
     4801 ctaaaagtaa ttcatcagcc attttttgaa tacgagcttg actctctgga gagcgactag
     4861 ccaataattc ttgtaagctc ttagccatta gattgatctc ctaaagttga gagatgaagt
     4921 gcgtattgct catctgcaat tgctagcatc tctgtataaa aacgcttttt attactctta
     4981 tcaccaatac acagcacaat cgcttgtctt aggggatcga atgcaaaaaa tgcccttaac
     5041 ggtttaccac gatgctgaac acgtagttcc ttcatattgg taaatttaga atcgtagacg
     5101 gtatctacta aaggacgacc taaactaggc ccttgctgtt gtagaacaac caatgcagct
     5161 aagactttct cttgtgttgc ttcatcttgc tgttcaagcc attcattaaa cagatccgtt
     5221 gtaatgactg tccacatatt acaatcacaa tatagattac agtctatata ttaaatctga
     5281 ttgaatgact ttgcaaatgg caataaaaaa tcccgacgtc atgtcgggat ttttcggtat
     5341 ggagcgcata ggtagggctg actacctgat ggtcatggtg cctgactgat tattattgtt
     5401 gttttaagtc attgaacatc ctgtccatat agatcgatca taagagcttc atcgggggat
     5461 agatacagac aatttcgtga aacgctgtaa gccaaaacgt acaaaaaata atcaaaattt
     5521 atcccagtgg ttatccacag aaatagtgga ttcttttggg taaaaaagct tataaactct
     5581 atggaattgc tatttttgat tattttttaa acaattttat tggcaagaag tatcaaaatt
     5641 gagcataaaa aaaccgactt gtttctaagt cggttgaacg aggtcgttcc agagggtatg
     5701 actcaatgta agctactgat cttaaagtaa tcttaaagat ccatttcgca taatatccat
     5761 tatgttaaat agtaggggac tctaagtaca agattagaaa atttagtctt taagaggaga
     5821 aatttgttct agcttatctt caggattatt ttctctaata tgtataattc gatcataatt
     5881 ttctactcct cgtaagtgag gtggtgcaaa tgctggatac ttgttgaaaa agtgcatttt
     5941 aatatttagt gcatggtcta aaataaaagc acattcaatt ccatagtact tatcattaat
     6001 aaaaaaattg tctgtagttt ctattagttc ttgaatagct aaatccaatt tttgaatttt
     6061 ttcattaata tctttagttt tctcttctat ctttttgtcc attggtatca cctatagaat
     6121 taagtttccc taaaattaac ataatacacc ttatgcgaaa ttccaatgta agcccaaaat
     6181 agaaatgtcc aacttctcta aaatccctgc taaataaact actcttcgcc tttttctaag
     6241 gcctttttaa tttcaattac tgacaataat tctccgccct tttgccaggt atgtaatcct
     6301 tctaacacct gaataaaaag tttatttgct ttaaaatatt cattttcata tgtcttaaca
     6361 tctttaaaaa ttaaagattt acgtacttca ttatgaaact ttgtcgtatt cacaatccga
     6421 ttggtaatct gttgaggagt atcattgcta atatcaatat tgagtttctt aaatgcgaca
     6481 agaacagcac cacctaattc aaatttacgt tgattttcag ctttgcgttt tttcccagca
     6541 tccttcttct gtagctgact caaatacaat tctaaatcac tgtattcaag cttttgcgct
     6601 ttaaaattca gataccgtgt cgccaaggtt tttaaaatat ttagttccat ctgatctagt
     6661 gcattctttt gaaaaagttc atacagatct aaaaacttct gttcatactc ggttttaaga
     6721 ggcagattat tcaactcttc aaacagcaat ttcaacttca gatgttcacg ataaggtttt
     6781 aaagcctctt tgaccagaac ctgatctgtc tgagttagcc cctcaaaatt acaatcaaca
     6841 ctatttaaag cctttaacac ttgagattga agatcagaaa gctgttcttg ctgttctaaa
     6901 aactctacaa atgcatgtaa ctcagcaatc tgtaacaact gagcaggtcg accacgcttt
     6961 tttgcttttt ccatttccat catctgccgc caaattttca gaactgcaac ctctttaaat
     7021 atggttttac catatttaaa gagggcagtc tgaaaataat tatttaatat aaaaataaaa
     7081 attcttttca agaaatttta tttttatatt aaataattcg cacttgacag tttctcctca
     7141 tcggagaaac tagtacgcaa tggggcgctt cccccttgac ccccttcaac cggatttttc
     7201 agccatacag gcatttcaaa tccgggcggt tttcattccg tttcactgca taaaaaccga
     7261 acccacaacc cagcagaggt gaacatggcc agctaccact tcagcgtcaa aagcaaaaat
     7321 aagggctatg cgaccggcca ttatctctac atttcccggc tgatgcagta tgaaggcatc
     7381 cgcaaaacca gcaatgagac catagaacat attgaacctg gtcgatgtat gcctgccttt
     7441 gtaaaagatc ccattgattt ttggcaggca gcagatacgt atgaacgagc caatgccaag
     7501 gcctacatgg agtatgaaat tgccctaccg aatgagttca cgtcagaaca gcgtaaaacg
     7561 ctcatagaga cgtttttaga caaacatata gttccacagc aatatccgca ctcatacgcc
     7621 atacacaacg taaaatcacg tatcagtggc gaagatcagc ctcattgtca tctgatgttc
     7681 tcgctaaaag cggatgacgg cattgaacgc agcgcagaac agtacttcaa acgctataac
     7741 cctcaagatc cagccaaagg cggtgccaaa aaaatccaac ttcaagatgg ccatgaagac
     7801 tattccactt ttttgatcta tatccgaaaa caatgggaaa atcatttgaa tgatgccctt
     7861 gcacagcact gcccgaccgt cacctacaca ctggatggcc aagacatcac catcaaaaat
     7921 caggtttcag cagacagcta tgaaaaatat aacgagatcc acagcacgct ataccttcca
     7981 gagccaaaac taggcgttgg acaacaaaat gcgacagcgg aatatctcgc ccaaatccaa
     8041 aaaatcagag aacacaacca gcaagaacga gaactcgaag aacgccaaaa gcaattagat
     8101 caacaacatg aatattttta tagtgctgaa aacaacccat tttcatcatt ggaagtcatg
     8161 tattacctga attacttaat caagaatgca acaaataagg acaaactaaa aaatgatttg
     8221 aatgaaatta tcaaaaacca aggccctata cttaaacaag aagcttggtc catctacgaa
     8281 atgaatgaaa aactagaaga ctcaagacgt aacagtaccc catgttttga gttcgaggtg
     8341 cattcacttc ctgaatacgt attaccgata caaaatacgc ctcccaaacg cactcaatca
     8401 gaaccagtac aacctaaagc tgatgattat gattacggtg gccctggttt ttaagctcag
     8461 ttggttttat ccaaagttta cagagcgagt gtctacgagc gaacagacac aattaaaatt
     8521 tttgccatgc tttttccctg attacaaact cccctttgct gctgagactg agatacagag
     8581 catcccggag ggtgcgaaca gatttaattt gcatctaggt cgagcgaagc gagtataaaa
     8641 aacttatgag cgaagcgaat tccgagttgc ttttgctttt ttgaagttat cacgagatta
     8701 acccaaaaat tgccccttcg aactgagcgt aagcgaagtt caatagagtt tgagcgtagc
     8761 gaaaacatag ggcaattttt cattcattgg gcttttaatt atttttaatc ttttaaatgc
     8821 ttttaaataa gctgtaaacc tttatttata atagtttcat agttaataaa actaggttta
     8881 tcgacccata aaactaggtt tatcgaccca taaaactagg tttatcgacc cataaaacta
     8941 ggtttatcga ccttaaagct aggtttatcg acccataatt aagttaattt acaactcaat
     9001 tgaaatagtc tattgtacaa cctagtttaa ttataaattt attattagaa atgagtgaat
     9061 taatcgtaaa ggataatgct ctaattcaag caagttatac cttagataca gttgaacaaa
     9121 gactgatcct attagccatt gctgaagctc gagaaactgg acatgggata actgaaaata
     9181 gtcttttaga agtacatgca agtagttata taaatacttt taatgtcgag aaacataccg
     9241 cttataccgt actcagagag gcatctaaaa gcttatttga tcgctatgtc acataccatg
     9301 acattaatcc taagacaggt aaggatcgta gctttcattg tcgttgggtc gacaaaattg
     9361 ggtatgaatc tcaatcagga attattttcc tacgattcac ccaagatatc gttccactca
     9421 taactcgact tgaagaaaat ttcactaaat atgaattgca gcaggtttct aggttaagta
     9481 gctcatatgc tattcggcta tacgagctat taattcaatg gagatctgcc ggaaaaacgc
     9541 cactttttga tctatctatc tttagacaac aacttggtgt caaacctcat caatacaaaa
     9601 caatgagtaa ctttaaaaca tatgttttag attttgctct taagcaggta aatgagttaa
     9661 ccgatataac agctaaatat gagcaacata aaaaagggcg ttctatttca ggtttttcat
     9721 tcactttcaa acagaaaaaa atgagtaatc tgccaataaa aaataagcgt gacccagaca
     9781 ctatagatat tttctcaaaa atgacagatg ctcaacgcca tctgttttcc cacaaactgt
     9841 cagaacttcc tgaaatggga aagtattctc atggtacaga aagctatccg caattcgctg
     9901 tacgcattgc agaaatgctt caaaacccag aaaagtttaa agaactctat ccttatctcc
     9961 aaaaagtagg cttcaaagct gcatagaaga tagacttgct gatttgtcta ttagacagtc
    10021 taatagacac taatttttta caagattagg cagagattta cacatgaaaa gccttacagt
    10081 tcttgagctt tctaagcttt acaatattaa tcgacaaacc atttacaaca atataaagaa
    10141 aggtatttta agtaaaaatt ctgacaataa gattgatctt gcagaagcga tccgggtatt
    10201 tggtgagcca gtacaaaaac agagtgtaaa agaaactgta aaagtagaca gtccaacttc
    10261 ggcagaagtt ttactcctta gacagcagat agacctacta aaaaatcagc tagatgatgc
    10321 aaaaaataga gaaagcttct atcaaaatca aattgaaact atgcaacgcc tattggaagc
    10381 tccaaaaacc aatacaacac aatctgatga tcctggatca acacaggcag aatccacagt
    10441 agaagaaaaa gaggacgtca tagctatgcc tcctaaggag tcagaggtta tcgagaataa
    10501 gcgtattcca gtccctgaac atattgagcc agaaccagaa aaaaggggct tctggagccg
    10561 ttttttcaag ccctatggat gataggacta tttaatcaga ctaatcaaaa ccgtggaaca
    10621 atgatcaata attgatcgtg aaacataaaa aatcactaaa acagcctgag cgtgactgct
    10681 taggctgttt ttacatccat aagacattgt tttaaaatgc tttttacaaa gaatttcgta
    10741 taaggtgtat tatgttaatt ttagggaaac ttaattctat aggtgatacc aatggacaaa
    10801 aagatagaag agaaaactaa agatattaat gaaaaaattc aaaaattgga tttagctatt
    10861 caagaactaa tagaaactac agacaatttt tttattaatg ataagtacta tggaattgaa
    10921 tgtgctttta ttttagacca tgcactaaat attaaaatgc actttttcaa caagtatcca
    10981 gcatttgcac cacctcactt acgaggagta gaaaattatg atcgaattat acatattaga
    11041 gaaaataatc ctgaagataa gctagaacaa atttctcctc ttaaagacta aattttctaa
    11101 tcttgtactt agagtcccct actatttaac ataatggata ttatacgaag tacacttgta
    11161 tattaattta aatatcaact acttaataaa aat
//
