LOCUS       NZ_CP103378             5589 bp    DNA     circular CON 17-SEP-2023
DEFINITION  Bacteroides thetaiotaomicron strain BFG-266 plasmid unnamed1,
            complete sequence.
ACCESSION   NZ_CP103378
VERSION     NZ_CP103378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30323249
            Assembly: GCF_024799865.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides thetaiotaomicron
  ORGANISM  Bacteroides thetaiotaomicron
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/16/2023 04:26:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,973
            CDSs (total)                      :: 4,886
            Genes (coding)                    :: 4,490
            CDSs (with protein)               :: 4,490
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 396
            CDSs (without protein)            :: 396
            Pseudo Genes (ambiguous residues) :: 0 of 396
            Pseudo Genes (frameshifted)       :: 344 of 396
            Pseudo Genes (incomplete)         :: 49 of 396
            Pseudo Genes (internal stop)      :: 24 of 396
            Pseudo Genes (multiple problems)  :: 19 of 396
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103378.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Bacteroides thetaiotaomicron"
                     /mol_type="genomic DNA"
                     /strain="BFG-266"
                     /host="Homo sapiens"
                     /db_xref="taxon:818"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1988-09-02"
     gene            255..755
                     /locus_tag="NXY38_RS24610"
                     /old_locus_tag="NXY38_24640"
     CDS             255..755
                     /locus_tag="NXY38_RS24610"
                     /old_locus_tag="NXY38_24640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_310506479.1"
                     /translation="MEAAKYVVAQWKKLHGSTVADARFQKYKKIEDFENAAIEVFKYAS
                     KASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFRTMIGDEALNDFRDEDL
                     VLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTRKDELLQDIYLDEDIFP
                     NSG"
                     /name="hypothetical protein"
     gene            complement(759..1220)
                     /locus_tag="NXY38_RS24615"
                     /old_locus_tag="NXY38_24645"
     CDS             complement(759..1220)
                     /locus_tag="NXY38_RS24615"
                     /old_locus_tag="NXY38_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812785.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            1312..2082
                     /locus_tag="NXY38_RS24620"
                     /old_locus_tag="NXY38_24650"
     CDS             1312..2082
                     /locus_tag="NXY38_RS24620"
                     /old_locus_tag="NXY38_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799241.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            complement(2151..3400)
                     /locus_tag="NXY38_RS24625"
                     /old_locus_tag="NXY38_24660"
                     /pseudo=""
     CDS             complement(2151..3400)
                     /locus_tag="NXY38_RS24625"
                     /old_locus_tag="NXY38_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NBU/pMV158-type mobilization protein"
                     /name="NBU/pMV158-type mobilization protein"
     gene            3806..4054
                     /locus_tag="NXY38_RS24630"
                     /old_locus_tag="NXY38_24665"
     CDS             3806..4054
                     /locus_tag="NXY38_RS24630"
                     /old_locus_tag="NXY38_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005812783.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4048..4368
                     /locus_tag="NXY38_RS24635"
                     /old_locus_tag="NXY38_24670"
     CDS             4048..4368
                     /locus_tag="NXY38_RS24635"
                     /old_locus_tag="NXY38_24670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_005799262.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            4405..4803
                     /locus_tag="NXY38_RS24640"
                     /old_locus_tag="NXY38_24675"
     CDS             4405..4803
                     /locus_tag="NXY38_RS24640"
                     /old_locus_tag="NXY38_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799260.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="hypothetical protein"
     Typing          2..755
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.868"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2586..3400)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.632"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaagtatt tggcttcgga aggcagagag ttttattttg tgacgcttac cttgcaaaat
       61 tgtgtaaccg atgatgcaag ggtattgcga gatttcatgc gtagatgtaa ccggatgtgg
      121 gctgatggta taaggacgaa acacaagttt cgttcacttg gtatttcggg ggtgattaag
      181 aaggagtgta cctatcatgt agtaaaaagg atttgttttc gttccattat cattttcata
      241 ttattgttga ttcattggaa gctgcaaaat atgtagtagc ccaatggaag aaattgcatg
      301 gtagtacggt tgctgatgcc cgttttcaga aatataagaa gatagaggat tttgaaaatg
      361 cagcgataga ggtttttaag tatgcttcaa aggcttctgt ctcaaaatcg aaaggtcgtg
      421 acggtaaaaa aaagattcag ataaattata aggctttgga tatgatttat actgccatgc
      481 atgggatgca gcgcatgagt agtttcggac agttccgcac catgatcgga gatgaagcgt
      541 tgaatgattt tagagatgag gatttggttc ttaatattga gggattgaat gttgaagatg
      601 gttgttatac ttggtatatg tctgtgaaag ataaggtggc tgattggtat aatatggaaa
      661 ctggggaggc attgtgttgt tataaggtca cccggaagga tgaacttttg caggatattt
      721 atttagatga ggatattttt cctaatagtg ggtagttgtt agtttgttgt attgttttct
      781 tggtatggtg tgagttctat gtattctcta ttcatgaaag gatcgccaaa ttttccactt
      841 ctgtcataga aggaaaattt gtttttagta agctcgtcta tgtcccagta atgttctcca
      901 ccaaaataaa tggtactgaa acttggatca ccgaatgacc attgtccgta gatattggct
      961 tcttttttat ttccttgttt gtcgatttct atatttgtac gggtatatgt tccgttttta
     1021 ttgaatttgt atgtaccttg ccagacactg ccgttttcgt ctgttgtttt tttaccagcc
     1081 catgtaatat cacaaattaa tgagattatg ctttcttctc catggttgta gtatccttgt
     1141 ttttcaacac atccggtgtt tgaaataaga aaaagtgata ctgttgaaat aagtaaagag
     1201 atatgtatta ttctattcat agttaaatat ttaatattca atgcaaaaat aggagaaatt
     1261 ttatattttt gggcgaaacc tttatttatt aactttaaaa aaggaggatt tatgaaaaaa
     1321 gtaatgttta tgttcatcag tctactgact gttttgggtg tttgttcgtg taatgataat
     1381 gaagtaatag aacctgtggt tagtgattct gtatctgata tggaggtctt atctcgtttt
     1441 gttgatgtaa atgagattac aaatgaatac tatttcaatg agaataagaa aacgagggcg
     1501 ttgtcttatg taacaggttc ggattggcag gatttagaaa aagttagtcc tttgagtatt
     1561 gagaaatata agaataacct gcaggtttta aatgctcaag tggcaagtgc tatttcaaat
     1621 cctaataccg cttatgttgt tttttctgta aatggaaaga ctttggttaa aaaggtgaag
     1681 gaggatgcta attttgattt ttcagtattt agggatgttg taacggaaac gagggctgtt
     1741 ctcccttctt tgagtattaa tggaggttct caaagcacta ccggagtttt ttacgattcc
     1801 agtcgtactt taaagatgca agttgatttg aatgcttcaa tacagaataa ttattatttc
     1861 tttgaagtat taaatcctaa tgctaaacca agtccggatg ataatataac gacacctgaa
     1921 agtgttgctt ttagtggaac tggaccgtta tggagtaaca ctttcacttg gacttcgtat
     1981 tgggatgcaa atgtacctgg tcaaggtttt aaatgggagt ttaaaggaaa aggtactact
     2041 ccttcttttg gttttatagc taattgcacc tttagtcgtt aaaaaatgaa cgggatttaa
     2101 tttgaattgc acttctcttt ttagagaagt gcaattcttt tagtgtcttt ctatcggtgt
     2161 agccagttac cgttatctaa agtcttttgt agtgtattcc aaagttcttt aagaaagttt
     2221 ttaaagtgga tgttatttag ccaaattgta gtaataccat tctgactttt ggagatatct
     2281 attttcacgt ttttaacttg gtatgatttt ctcttgtctt tatcgtaaag gtttccagtg
     2341 tagtatattt cctttccaat aagaagttgt cggatatctt tattttgtat tcccatagtt
     2401 tggagttcct ctatattttc ctgtgcattt tccatttccg gaaaaaggtt agttatgggt
     2461 tttagtttgg atgcttctgc ctttatctgt ctgtcggttg tttcctgtac ccttcgcatc
     2521 tcgatgttgt tggttcttaa ttgtccttct aaattttgat tcttgaatga gaggttataa
     2581 ttttcttttt gagggtttca atttctttgg cttgtcgttt tacttcccct acattaaaga
     2641 cgcttgtaac ggcattaaat acggtttttc cggcttttgc ggcagcttct ttggcttcgt
     2701 tgatgtgtat gtcggttttc atgctgttta gctctttttg tgccgtacta agttcctttt
     2761 ggactgtggt gattttctcg gttccggcag tgtatgccct gtttagttgc tcgatgtctt
     2821 tttgaagttg ttcagctttc tctttttcca ttttgttttt gtattgcatg gccgacaagt
     2881 gtttcttgtc tgaagaaact ccacgttcca tgttgagaca ctctgctgtt atagtctgca
     2941 tctccgccat gtcgtgtctg ttcagcttta cagtttttcc tgtatggccg tccgtccagt
     3001 caaagatcat gtgcgcatga tagttcggtt tccatgcttc ttcctttccg tcccatacgt
     3061 ttgcgccttc atccttatgg gtgtagattt ggaaggcgtg cactccaaag cgttcttcca
     3121 gtttttcggc caagtcttga agctgctgga ttgtggtttc ctcgctgatt actattaccc
     3181 cttcccggat tggggtagct ttcttctgta atccttgtcc ggtggcttcc ttgtattttt
     3241 ccgttatgtc ccggtgtact tcggcaatgg aacattctac ccactgttcg ttgcggtgtg
     3301 tgagttccgg acggatgtag tcgagctcct tgcttcttaa gttgtgacgt tccgagctgc
     3361 ctatattgca ggcttccact ctgatacatg tctttgccat atctttaggt tttaggtgtg
     3421 tatgtatgtt tagaagatgt ggcgtgaaac gtcacatcgc acggggtcga ggggacaccc
     3481 ctcgcacaca acccctttag gggttatagt gtgctataac cttttgcaaa ggtaagcctt
     3541 ccggacggga tttgcaagcg ctctgccatg tgttccggat gtgcttggga acagaagccg
     3601 gacaaaaggg aaaaggacac aaggcacaat aaaaaatcat cggtcgggga gcgctgcacg
     3661 gcaagggggt gcaccaacac tcccgacgag tgcagccatt gacagggttc tcggtgaaaa
     3721 tggaaaaaag ttgtttttcc ttttgttatt gttataactt tacatatatt tgtatcgtga
     3781 taatcaatta taaagttata acattatggt aacgaatatt attcagattg gaaacagcaa
     3841 ggggataata cttccttccg aagtattgaa acagttgcgt ttgtccttga aatcggctgt
     3901 gagcatttct ttggacggga acaatattgt gataaaggca caaccacggc agggatgggc
     3961 ggaagctgcc aaacgtgccc atgagaatgg ggatgatgag ttgcttatcc ctgatgtgtt
     4021 tgaggatgaa aagtttgagg attggacatg gtagaacagt atgaagtgta ttgggtagag
     4081 ttagacccta cccgtggggg tgaaatggca aagacacggc catgtgtagt tgtcactcct
     4141 tcagatttga atatgtacct tacaacagta gtcattgttc cgataacctc aacgataaga
     4201 aattatcctt accgggttct gtgttctgta gccgggcgtg aaggagagat agccactgat
     4261 caaattcgca cggtggataa aagccggctg aaaagaaaga ttggagattt gaatttcagt
     4321 gagataaaaa ggttacgtga agtgtttcag caaatgttct gtgaataatg gaaagtacgg
     4381 aaagaaaaat gcaaataaga cattatgaaa ccaactgact acatcgaatg ggataacctg
     4441 aaggatattc cattcttcct ctgtcaagta gtcgaggaca gagaaaaaca agatttggat
     4501 atttactatt tgggcaaacg tgtcttgcac gattacgacc atgtggggca ctatttgcgg
     4561 actgccgtta tcttgttccg cagggtaaag agccgcacag cagactgggt aaacctgcgt
     4621 aacctttgga cgctgcgcaa ctgtgtgcgt gagaactaca atcacggcat aggaatgaac
     4681 gaccttattt ttggggagaa tttcgatgga gataatctcg atacgcttac accacttaca
     4741 aagaaacgct ttgatttttt gtgtaagcgg attaaggaac ttgacccata tgcaactatt
     4801 taacactcca ttgagtgaag gcagggcttg tctttgtgtg ctcccccttt gggggacggg
     4861 agggggataa gaaaaaaata ccaccggaga tgtcggatat ccgtcaattc actccggtgt
     4921 tttttttgca actctcccgt atcgctcggc aagggttgtc tttgtgtacg gcaaagatag
     4981 ttttttttcc ggaagtatct ttgtctgttg tggatttagt agtgtatttg tgtcagaatt
     5041 gcgcagccgg gggaatgggg gcgagccccc atactagata aactagaaag tttcgtggta
     5101 aatgcttttt gacttgttat atgtaggttt tttgaatgat gtttggcggt ttggaatgaa
     5161 ttacctactt ttgggctgag aaaaaacgaa agcccgaagg cagtaaccaa cgggctttgt
     5221 attttaagaa ttagcaatcg tttgtgttcg ttcttggcgg ggcggatacc aaacgaaagc
     5281 cacatcaaaa tttcatatgt gcaaagatag aacaaatata accaactgca aagaaagtcc
     5341 gttaaaaaac ggttctgata gtcttaaaaa aaggctaaag taaaaatcat atcccataat
     5401 tttactcgaa aactttcgga aaagaatccg gatagtaagc tgatgaaagc atatatggat
     5461 gcccatgtgt gttcaaatgt ttatactgtg cagggaggta aggcgcattc ccattattgt
     5521 catcgggctt tttgtcctat ctgtcagcgt attcaaacgg cacaaaatgt aaagaagttc
     5581 cttccggtt
//
