LOCUS       NZ_CP103341            13439 bp    DNA     circular CON 02-OCT-2023
DEFINITION  Acinetobacter baumannii strain AB105 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP103341
VERSION     NZ_CP103341.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30068511
            Assembly: GCF_025264005.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   Guo,T., Yang,L. and Li,G.
  TITLE     Whole genome sequence of AB105 and the relationship with MGEs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13439)
  AUTHORS   Li,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2022) School of Medicine, Yangzhou University, 88
            Daxue South Road, Yangzhou 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/02/2023 04:16:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,674
            CDSs (total)                      :: 3,579
            Genes (coding)                    :: 3,414
            CDSs (with protein)               :: 3,414
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 65 of 165
            Pseudo Genes (incomplete)         :: 85 of 165
            Pseudo Genes (internal stop)      :: 40 of 165
            Pseudo Genes (multiple problems)  :: 20 of 165
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103341.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13439
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AB105"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2022-04-20"
     gene            1..933
                     /gene="repM"
                     /locus_tag="NT399_RS18230"
                     /old_locus_tag="NT399_18230"
     CDS             1..933
                     /gene="repM"
                     /locus_tag="NT399_RS18230"
                     /old_locus_tag="NT399_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021644.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_002124966.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANNP
                     LEVHAESYINQFNVARQTAYQALKDASKDLFARQFSYQEMNKRGNIENVLSRWVSEIRY
                     IDAEATVKLIFAPAIVPLITKLEEQFTKYELQQVSNLSSAYAVRLYELLIAWRSTGQTP
                     VIELEEFRKKIGVLDDEYTRMGNFKDRVLHLAIAQVNEFTDITVKYEQHKKGRSIYGFS
                     FSFKQKKNVNKPNLEARDQNTLDIFTKLTDAQRHLFANKLSELPEMSKYSQGTESYPQF
                     AVRIAEMLLDAEKFKELYPYLVKVGFQTK"
                     /name="repM"
     gene            993..1553
                     /locus_tag="NT399_RS18235"
                     /old_locus_tag="NT399_18235"
     CDS             993..1553
                     /locus_tag="NT399_RS18235"
                     /old_locus_tag="NT399_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_223154292.1"
                     /translation="MNTTKFSVMDASKAFKKSRTTIYEALKNGELSRDNDGLIDLSELI
                     RVYGNPIGVQSSTRTEQVQKDVQVHVQSEVENLLKDQISLLKNQLDLANQREKSLMQHI
                     EDLTHRIEFKGTLEQSQQENIDKLNESTNSNIATDPRSQTDSNYDELTTSESKRIHLPE
                     HVEPEPKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1566..1709
                     /locus_tag="NT399_RS18240"
                     /old_locus_tag="NT399_18240"
     CDS             1566..1709
                     /locus_tag="NT399_RS18240"
                     /old_locus_tag="NT399_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125247.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDERQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            1899..2282
                     /locus_tag="NT399_RS18245"
                     /old_locus_tag="NT399_18245"
     CDS             1899..2282
                     /locus_tag="NT399_RS18245"
                     /old_locus_tag="NT399_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_038343695.1"
                     /translation="MWKIYKEESSDLGFAIGCLFSEAINLNEFRQWVELCINDLNQDEI
                     PLYMFDLLDFEDSLAKIHLVIGFVPSSGLSKTEKIALEGISYLRGIDIFDPMSTQEQSI
                     EYLKNNPHVSEKFKLTFPFINFI"
                     /name="hypothetical protein"
     gene            2512..2799
                     /locus_tag="NT399_RS18250"
                     /old_locus_tag="NT399_18250"
     CDS             2512..2799
                     /locus_tag="NT399_RS18250"
                     /old_locus_tag="NT399_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            2786..3097
                     /locus_tag="NT399_RS18255"
                     /old_locus_tag="NT399_18255"
     CDS             2786..3097
                     /locus_tag="NT399_RS18255"
                     /old_locus_tag="NT399_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_004836815.1"
                     /translation="MSMVRYTRKELNENFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNH"
                     /name="BrnA antitoxin family protein"
     gene            complement(3204..3665)
                     /locus_tag="NT399_RS18260"
                     /old_locus_tag="NT399_18260"
     CDS             complement(3204..3665)
                     /locus_tag="NT399_RS18260"
                     /old_locus_tag="NT399_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002124952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002124952.1"
                     /translation="MIDFSVDINDLVAQLERDSTVSIEEKKQILEVLNDETDDEQLSIK
                     MEVLAKDFLGEDSTTQEEWALVSGGDRGSFVDEYKKQIYKLICTESEEYKEDRLALSSH
                     SKSLLQIAISIATTTYSVSLAVVSATIYCILIPILKLGKNAYCSMHHVS"
                     /name="hypothetical protein"
     gene            complement(3658..4359)
                     /locus_tag="NT399_RS18265"
                     /old_locus_tag="NT399_18265"
     CDS             complement(3658..4359)
                     /locus_tag="NT399_RS18265"
                     /old_locus_tag="NT399_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002124954.1"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]; GO:0008236 - serine-type peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="WP_002124954.1"
                     /translation="MDSIFQKFNSKTIALLGVLEKNETTHESNVLFRGTCFKIYQNYYL
                     TCFHVLDLSKKMVISFPNEIDGFFDTKKKQSFQCINIRVISKDVKNDIALIKIVGNYLH
                     YGYEPKPIDLIDNTISKIGNDVLYAGYPFCHQEMTSLKWSKSIISSKILSSKATKQYML
                     DSVVHQGNSGGPLIDSNTGKIIGIISMTYNPMFGSGIMFGNQALSTTTSLGMAISITHA
                     INLLKTANIYD"
                     /name="serine protease"
     gene            complement(4536..4808)
                     /gene="nadS"
                     /locus_tag="NT399_RS18270"
                     /old_locus_tag="NT399_18270"
     CDS             complement(4536..4808)
                     /gene="nadS"
                     /locus_tag="NT399_RS18270"
                     /old_locus_tag="NT399_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_002126333.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIQASRTTELKLPDIKEVREKTG
                     LSQSEFAARLHISHRTLQNWEQGRRYPTGPAATLIRILDAHPSLI"
                     /name="nadS"
     gene            complement(4801..5121)
                     /locus_tag="NT399_RS18275"
                     /old_locus_tag="NT399_18275"
     CDS             complement(4801..5121)
                     /locus_tag="NT399_RS18275"
                     /old_locus_tag="NT399_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_002126330.1"
                     /translation="MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVNEDDQIFFLVAYPKSVKDNLTDKETAILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5293..5721
                     /locus_tag="NT399_RS18280"
                     /old_locus_tag="NT399_18280"
     CDS             5293..5721
                     /locus_tag="NT399_RS18280"
                     /old_locus_tag="NT399_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002125445.1"
                     /translation="MKYSNEKIVKALLLSPLPLLFFTTVLFIVMNQEYSLYSILVVLVG
                     HGFVYLAYCILTVPFSFIFSILLNRYNSLNLLTICIASIIIATPFFILFGWSHTGEISK
                     EWWKMYTDTWTIFMALFPGLCYWLFLINLKDKKSKNTE"
                     /name="hypothetical protein"
     gene            complement(5787..5978)
                     /locus_tag="NT399_RS18285"
                     /old_locus_tag="NT399_18285"
     CDS             complement(5787..5978)
                     /locus_tag="NT399_RS18285"
                     /old_locus_tag="NT399_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002125028.1"
                     /translation="MSTELVNSANIISFPKPCAFCESTEHVQLFAGLMLCKSCQENIKI
                     TNPDLFATNEQIQQKAQD"
                     /name="hypothetical protein"
     gene            7347..8270
                     /gene="repM"
                     /locus_tag="NT399_RS18290"
                     /old_locus_tag="NT399_18290"
     CDS             7347..8270
                     /gene="repM"
                     /locus_tag="NT399_RS18290"
                     /old_locus_tag="NT399_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_000845851.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNIINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITLSDFRQKIGVLDTEYKRMYDFKKYVLDIALKQVNEHTDITVKVEQHKTGRSITGF
                     SFSFKQKKSVTKSAKSIGVSEDITITLTDAQRYSFASKLSELPEMGKLSQGTESYEQFA
                     VRIADMLKQPEKLKELTPLLRKVGFQ"
                     /name="repM"
     gene            8338..8883
                     /locus_tag="NT399_RS18295"
                     /old_locus_tag="NT399_18295"
     CDS             8338..8883
                     /locus_tag="NT399_RS18295"
                     /old_locus_tag="NT399_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000736775.1"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFAEAIRV
                     FGEPQKKDTNVNQSQSINSQNLTEVDLLKRQVDILEKQLNQALFRENQSLERESFYQDQ
                     IEAMQRLLEAPKANMTTFTDQEQSQANVAQVQPEPNIDDPKHDGLTTPENKRIPVPEHV
                     EPEPKKRGFWSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            9176..10696
                     /locus_tag="NT399_RS18300"
                     /old_locus_tag="NT399_18300"
     CDS             9176..10696
                     /locus_tag="NT399_RS18300"
                     /old_locus_tag="NT399_18300"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249302.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_049590605.1"
                     /translation="MSQDPKKCPVTHLTTEAGAPVVDNQNSMTAGARGPLLAQDLWLNE
                     KLANFVREVIPERRMHAKGSGAFGTFTVTNDITQYSRAKIFSEVGKKTEMFARFSTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDLVGNNTPVFFLRDARKFPDLNKAVKRDPKTNKR
                     SATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTFSFINAQNERFWVKFHM
                     RTQQGIQNLTDAEAADLIAKDRESSQTDLFDAIERGDYPKWKMYVQVMPELEAETVPYH
                     PFDLTKVWPKGDYPLIEVGEFELNRNPENYFQDVEQAAFAPSNLVPGISYSPDRMLQAR
                     LVNYADAARYRVGVNHSQVPVNAARCPVNSNRRDGQGRMDGNYGSLPHYEPNSFNQWQE
                     QPQFKEPALKITGDADFWDFREDDNDYFSQPRALFNLMNDEQKQALFNNTAAAMGDALD
                     FIKYRHIRNCYACDPAYGQGVAKALGMTVADAQAARETDPAKGLPSFL"
                     /name="catalase"
     gene            complement(11182..11709)
                     /locus_tag="NT399_RS18305"
                     /old_locus_tag="NT399_18305"
     CDS             complement(11182..11709)
                     /locus_tag="NT399_RS18305"
                     /old_locus_tag="NT399_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_002124964.1"
                     /translation="MGTVKLKSSDEVFQLKISLLGSDPEIWRTIQISSAATLSRLHKAI
                     QSAFDWEDSHLHEFTTIKHEKINKRQKLKEVLRVGKKIIYTYDFGDCWEHLITVESRQS
                     PDLNKKYPCCIDGQNHAPFEDIGGIFGYLDILDALQEPKHPRYDDYMQIIEDEIGEIEF
                     DPTYFNSHDIKF"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            complement(11820..12188)
                     /locus_tag="NT399_RS18310"
                     /old_locus_tag="NT399_18310"
     CDS             complement(11820..12188)
                     /locus_tag="NT399_RS18310"
                     /old_locus_tag="NT399_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_025467239.1"
                     /translation="MTEQKKPKRIRQKVIQVVVTEAEKKQIEENAAQGNLSASQYLRDL
                     GLGYQPKSLTDVKTIQEIRSLKADMNKVGGLLKNLMNGELTDPKEIRSQVNGLLLDFSK
                     NQNSIDLFISKVRSAIKF"
                     /name="LysR family transcriptional regulator"
     gene            12475..12810
                     /locus_tag="NT399_RS18315"
                     /old_locus_tag="NT399_18315"
     CDS             12475..12810
                     /locus_tag="NT399_RS18315"
                     /old_locus_tag="NT399_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002124970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002124970.1"
                     /translation="MVTRHGAKKFIISVLPEIEKRLEAGYTIKEIHADLPELANNISYS
                     SVLRNLSKMGISEKKPHSNKIEKSVNLDTASSSSQKITNAQRNLEKLQKRPEKFKFDHG
                     TNDKELI"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1247"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.143"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7348..7951
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.835"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcgagatc tagttgtaaa agataatgct ttaattaacg caagctataa cctagatcta
       61 gttgaacagc gacttattct tttagctatt gttgaagcaa gagaaagtgg taaaggcatt
      121 aatgcaaaca accctttaga agtgcatgca gagagctata tcaatcaatt caatgttgca
      181 agacagactg cctatcaagc attaaaggat gcttcaaaag atttatttgc tagacaattt
      241 agctatcaag agatgaataa acgaggaaac atcgaaaacg tactaagccg atgggttagt
      301 gagattcgtt atattgatgc tgaagcgact gttaagttaa tttttgcacc tgctattgtt
      361 ccattaatta ctaaactcga agaacagttc actaagtatg aattacagca agttagtaat
      421 ctcagtagtg cttatgctgt acgcctatat gaattattga tcgcatggcg tagcactggc
      481 caaactcctg ttatagagct tgaagagttt agaaaaaaaa ttggtgtgct tgatgatgag
      541 tacacaagaa tggggaactt taaagacaga gtcttacatc tagctatagc tcaagttaat
      601 gagtttacag atatcactgt taaatatgag cagcataaaa aaggacgttc aatttatggc
      661 ttttcattct cattcaagca aaagaaaaac gttaacaaac caaatctaga agctagagat
      721 caaaacacct tagatatttt caccaagtta acagatgctc agcgtcattt atttgctaac
      781 aaattgtcag aactgcctga aatgagtaag tattctcaag gcaccgaaag ctatccgcaa
      841 tttgccgtac gaattgccga aatgctatta gatgctgaaa aatttaaaga actatatcca
      901 tatctagtaa aggttggctt tcaaacaaaa taaagataat gttcagtaat atgctcacct
      961 gaacatcctt gtacatgaac agagggattg tattgaacac tacaaaattt agcgttatgg
     1021 acgcaagtaa agcctttaaa aaatcacgga caacaatata tgaggcttta aaaaatggtg
     1081 aattatcaag agataatgat ggtctaatag acttatctga acttatcaga gtttatggca
     1141 acccaatcgg tgttcagtcc agtacacgta ctgaacaagt tcagaaggat gtacaggtac
     1201 acgttcaatc tgaagtcgaa aatctattaa aagatcagat ttctttacta aaaaatcagt
     1261 tagatttagc aaatcaaaga gaaaagtctt tgatgcaaca tattgaagat cttactcata
     1321 gaattgagtt taaaggcacc ttagaacagt cacaacaaga aaatattgat aagctaaatg
     1381 aatctacaaa ttcaaatata gcaacggatc ctcggtcaca gactgactct aactatgacg
     1441 aattgactac ttccgagagt aaacggatcc atcttcctga gcatgttgaa ccagaaccta
     1501 aaaaacgtgg tttatttggc cgtgtactaa atgctgtttt tgataatgac taagggagag
     1561 agatcatgcc gaaactgaaa gacattgccc tgggaattat tgtggccccg ctgctgatcc
     1621 cgatcatgtt gattgcatcg taccaggata aaaaggcact taaaaaagag ctggatgaac
     1681 gccaaaaaga aaaagaccag gcatcctgat tattcaattg aactaatcaa agtcgtgaaa
     1741 ctttgattaa ataataaacg tggaacgtga aaaatcataa aaaaagcccg aacagggatg
     1801 ttcgggctat aaactagaaa ctacagcatt gatttctata gataattata acgcatttcg
     1861 tataaggtgt attatgttaa ttttagaaaa atttaagtat gtggaaaata tataaagaag
     1921 aaagttctga tcttggattt gctattgggt gtctattttc agaagcaatt aatttaaatg
     1981 agtttaggca atgggttgag ctttgtatta acgatttaaa tcaagatgaa ataccacttt
     2041 atatgtttga tcttttagat ttcgaagact cattagcgaa gattcattta gttattggat
     2101 ttgtgccaag ctctggacta tcaaaaacag aaaaaatagc gttagaaggc atttcttatc
     2161 taaggggaat agatattttt gaccctatgt ctacgcaaga acaatccatt gaatacctaa
     2221 agaacaaccc acatgtctca gagaaattta agctcacttt tccttttata aattttattt
     2281 aacataatgg acgttatacg aaatcgtctt ttgtaagtta aataaaatca tatacttagc
     2341 tattttacat aacccgaaac taccgcaaaa atccgctttg gaaacaaagc ggattttttt
     2401 tgagcaagtt tgatacttct tgccaataat tttgactaaa aattactcca attagcttga
     2461 gtgatacaaa tttatgatac attattgtat atacaaattt gaatgttttt tatggaactg
     2521 tatttcgaat gggatgaggc taaaaaccaa aagaatcaga aaaaacatga tgtatctttt
     2581 gaaacggcaa gcctcgtttt tgaagatcct ttacggatct caatccagga tcgacatacc
     2641 aatggtgaag aacgttggca aacgatcggt atggttaaag gtgtactgat gttattggtg
     2701 gctcatacaa tcattgacga tgatgactgt gagatcatac gaatcattag tgcaaggcaa
     2761 gtcactaaag cggaacgcaa tcaatatgag catggttaga tatacacgca aagaactgaa
     2821 tgaaaatttc agtgacaagc aagatgctga aattaaacgc ttgcttgcga aaggaactgt
     2881 accagatgaa caattagatc tatctgacat tcctgagatt accgactgga gtcatgctgt
     2941 tcgccatggg caattttatc gtccagtgaa gcaacaaaca tctgttcgtc tagatgcaga
     3001 tgtacttgca tggttaaagg ctcaaggcaa aggctatcag acacgcatga ataaaattct
     3061 gcgtgaagca atgctagaag atcttaagaa tcattgatga aaatggaagc tatagactcc
     3121 catttttgct tcgcataaga gtttttatgt taaatagccc tcaattgagg gccacttatt
     3181 atttttctat ttgagaaaat tgattatgaa acatgatgca tactgcaata tgcatttttt
     3241 cctaatttta atataggaat taaaatacaa taaattgttg ctgaaactac agctaagctt
     3301 acgctatatg ttgttgttgc tatagatatt gcaatttgaa gtaaactttt agaatgagat
     3361 gaaagagcta gtctatcttc tttatattct tctgattctg tacatattag cttgtatatt
     3421 tgttttttat actcatcaac aaatgatcct ctatcaccac cactgactaa agcccattct
     3481 tcttgagtag ttgaatcttc accaagaaaa tctttagcca agacctccat ctttatactt
     3541 aactgttcat catcagtttc atcatttaac acctctagaa tttgtttttt ttcctcaatg
     3601 ctgacagtag aatcgcgttc taattgagca actaaatcat ttatatctac agagaagtca
     3661 atcatatata tttgccgttt ttagtaaatt aattgcatgg gtgatggaaa tagccatgcc
     3721 taagctagtg gttgtagata gtgcttggtt accaaacatg attccacttc caaacatcgg
     3781 gttatatgtc atagaaataa tgcctataat ttttcctgta ttagagtcta ttaatggccc
     3841 gccactgtta ccctgatgca ctactgagtc caacatatat tgtttagttg ccttagaact
     3901 cagaattttg gaggatatta ttgatttaga ccattttaaa gaagtcattt cttgatgaca
     3961 gaatgggtat cctgcataaa gcacatcgtt tccaattttg ctaatagtat tatctatgag
     4021 atcgataggt tttggttcat atccataatg aagataattg ccaacaattt taattagagc
     4081 aatatcattt tttacatctt tagaaattac tctaatatta atacattgaa atgattgctt
     4141 ttttttggta tcaaaaaaac catcaatttc atttggaaat gagataacca ttttttttga
     4201 taaatctaaa acatgaaaac aggttaaata ataattctga taaattttga agcatgtccc
     4261 tctaaataaa acgttagatt catgcgttgt ctcgtttttt tctaagactc ccaacagtgc
     4321 tatagtttta ctattaaatt tttgaaaaat actatccata tagaggttgg gatctttatg
     4381 tttgaatggg atcgattata aggaataatt tttaatttca ttaaatatat ttcaattact
     4441 taaattttta atggtttaga aatattaaaa ttaacataat acaccttata cgaattcaaa
     4501 aaatgggagc taagctccca tttttaatgc attttttaaa taagactagg atgtgcatct
     4561 aaaattcgaa tcaatgttgc tgctggtcct gttggatagc gtcggccttg ttcccagttt
     4621 tgcagagtac gatggctaat gtggagacgt gctgcaaatt cgctttgact taaaccagtt
     4681 ttttctcgca cttccttaat atctggtaat ttaagttcgg tcgttcgact agcttggatc
     4741 tctttgcgct tgattgctcc tgcttcttta atcgaagcaa ctaagtcatc aaataagttg
     4801 ttatccatga aattgctcct tcacgagttg gcgcagaatg gcggtttctt tatccgttag
     4861 attatctttt acagattttg gataagccac caagaaaaag atttgatcat cctcatttac
     4921 ccaatagtaa attactctga taccgccact tttgccttta ttcccttgag cacaacgtac
     4981 ttttcgaatg ccaccgccat tcttgattaa gtcaccttta tctggctgaa ccaatagatc
     5041 ttgttgaagt tgacgatatt cctcatcaga tacaaggtct ttgatttgct tggtaaagat
     5101 actggtttca atgaataaca tagattctat gtacgtcaat ggcgtacaag tattctagca
     5161 gtttccacat taaaaaacga gagctgctgc tctcgtttcg tataagcacc attatgttaa
     5221 atataatcaa aaaacctcca ataaaatcgt tatagtatta ttatttattg agtagataag
     5281 agaattaaat tagtgaaata ctcaaatgaa aaaatagtaa aagctctctt actttctcct
     5341 ttaccactac tttttttcac aacagtcctg tttatcgtaa tgaatcagga atacagccta
     5401 tactcgattt tagtggttct tgtagggcac ggttttgtct acttggctta ttgtattttg
     5461 actgtacctt tcagcttcat attctcaatt cttttaaatc gttataacag tcttaattta
     5521 ttaactatct gtattgcctc tataattatt gctactccat tttttattct ttttggatgg
     5581 agtcacacag gagaaatttc gaaagagtgg tggaagatgt atacagatac atggactatc
     5641 tttatggctc tatttcctgg cttatgttat tggctatttc tgattaacct aaaagataag
     5701 aaatcaaaga atactgaata aaccattgca tagagattct taaatttccc taaaattaac
     5761 ataatacacc ttatacgaag cccaggttaa tcctgggctt tttgttgaat ctgttcattg
     5821 gtagcaaaca gatcaggatt ggtaattttg atgttttcct gacaactctt gcaaagcatc
     5881 agcccagcaa aaagttgtac atgttccgtt gattcacaga aggcacatgg cttaggaaaa
     5941 gagatgatat tggctgaatt gactaattct gttgacataa gaagtaacct tggattgtac
     6001 atatttattt ttaataaatt tcaatacttt atacctaatt tagccactaa aatttgtaca
     6061 ttattgtata atccatgtgt acatattatt ttttataatt ttcattcact tagacccaaa
     6121 atagtattta tttttgtaca acaccatgta cagaacaata atcatataaa tcaaattttt
     6181 agcataaaaa agtccattaa ttttgtacac aattctgaaa cttaaaaatc taaactttca
     6241 tcaatttttt tcataatttc aataaattaa ccttaatttt aagatatatt ctgaaatttg
     6301 ggttgaaagt tgtttttaca ttatatttca atactttaaa tcaaaaaatt ggattttttt
     6361 tgaaaaactc tatgaaagtt tattttttat ttaaaaacaa ctagttatat tagtttttat
     6421 ccattttttt gaaacagttt ctatatgata aaaaaaccta taaaaaccat atctagcaaa
     6481 ggtttgaggg ttatcatctt tagatgcgtg gtgtgtgaca aaaaaatccc ggcatgtgcc
     6541 ggattctgga ttagaaaatt ggctaaagtg acgtaggact ggtgcttggt tttacatgga
     6601 aaaaagtatt tattttctgg tttataaaaa cgtaaaaaga tcagtttttg ttcattcatc
     6661 caggttaaaa atttcaacct aaaactttaa ttatgaaaag cttcacagaa agcattcaaa
     6721 tgcgatttaa gagcctttat ctaaaaaaca tagatcttat agcgaaaaac agaaaacagc
     6781 tcaaaaaacg caaaagagag tgaagtaaag agatgttttg actttagata gctgcataga
     6841 gcgagtgtct acgagcgaac tatcaaaatt tgcgtctaga ctctctgaaa cacatttttt
     6901 tgccctcttt agcctaagaa agcttaattt tcatgcagaa atttgctcct ggaccgagcg
     6961 tagcgagaaa aaaagctcat gagcgaagcg aattccgagt tgcttttgct ttttcttaaa
     7021 gtcacgcaag tattaaccaa aaaattgccc cgatgaactg agcgaaagcg aagttcaata
     7081 gagtttgagc gaagcgaaaa ccaagggcaa tttttcatga cctgagcttt taaatatttt
     7141 taaatctttt aaatgctttt agacatgctg aaagccttta tttataaaat tcttagagac
     7201 tataaaacga ggtttacctt gcattaaaac gaggtttacc ttgcattaaa acgaggttta
     7261 ccttgcatta aaacgaggtt taccttgcat taagcgagtt tatgatatag cctcgtctta
     7321 ttgaattaaa aatataacct tttcatatga aaacagaact aatagttaaa gataatgcat
     7381 taattaatgc cagttataac cttgatctag tcgagcaacg gttaattctt ctagctatcg
     7441 ttgaagcaag ggaatcgggt aagggaataa atgctaatga tccattaaca gttcatgctg
     7501 aaagctacat caatcaattt ggtgtacatc gaaacacggc ttatcaggca ttaaaagatg
     7561 cttgtgatga tctatttgca agacaattca gttatcaaag tcttagtgaa aaaggcaaca
     7621 ttattaatca caagtcaaga tgggtgagtg aagtcgctta tattgataat gaagcggttg
     7681 ttagacttat ttttgctccc gctatcgtgc ctttaattac aagactagaa gaacaattta
     7741 caaagtatga aatacaacaa ataagcaatt taacaagtgc ttatgccgtt cggttatatg
     7801 agatattgat tgcatggcgt agtacaggaa aaacgcctct tataactttg tctgatttca
     7861 gacaaaaaat aggtgtactc gatactgaat acaaacgaat gtatgacttt aaaaaatatg
     7921 tcttagacat tgcattaaag caagtaaatg aacataccga cattactgtt aaagttgaac
     7981 agcataagac tggtagatca attacaggtt tttcatttag ctttaaacaa aaaaaatcag
     8041 taacgaagtc agctaaaagt ataggtgtaa gcgaagatat aacgatcact ttaacagatg
     8101 cacaacgcta ttcatttgcg agtaaattgt cagagcttcc agaaatggga aaactttcac
     8161 aaggcaccga aagctatgaa caatttgctg tacggattgc agatatgcta aaacagccgg
     8221 agaaattaaa agaacttact ccattacttc gaaaagttgg ctttcaataa atgatgtcaa
     8281 ttgacattgt aaagttgaca gtcaagtatt aacacaaatt gaaaactggt ttacataatg
     8341 aaaaaactgt cagtttcaga gttggctaag ctttacggat attcacgaca agccatatat
     8401 gcccatataa acaaaggaaa tttatctaaa ggatctgatg gattaattga cttcgcagaa
     8461 gccataagag tttttgggga accacaaaag aaagatacta atgtcaatca aagtcaatca
     8521 attaacagtc aaaacttgac agaagttgac ctattaaaac gtcaagttga catactggaa
     8581 aaacagttaa atcaagcact gtttagagaa aatcaatcac tagaacgtga atcgttttat
     8641 caagatcaga tcgaggccat gcagcgctta ctagaagcac caaaagccaa tatgactacg
     8701 tttaccgatc aagaacagtc gcaagcgaat gtagctcaag tacagccaga acctaatatt
     8761 gatgatccta aacatgacgg attgactact ccagagaata agcgcattcc tgttccagag
     8821 catgttgaac cagagcctaa aaagcgtggc ttctggagtc gtttttttct tccatatggt
     8881 taacttaggc taaataaaac cgtggaacat tgctcaataa ataagcgtgg aacgtgaaaa
     8941 atcataaaaa aaacccacct tggggaagat gggctataaa ctagaaacta cagcattgat
     9001 ttctaaggaa aattataaca catttcgcat aagagttttt atgttaaatc agtcggctat
     9061 aaattttcaa aaatatcctt agtttaatat tttaataagc aagtaaaggg taagatagga
     9121 ttgaaaatct taaatctcta ggaaatttaa attaaaagaa acgggagtta atagtatgag
     9181 tcaagaccct aaaaaatgtc ctgttaccca cctgactact gaagctggtg cccctgtggt
     9241 cgacaatcag aacagtatga cggcaggtgc gcgtggacct ttacttgccc aggatttatg
     9301 gctaaatgaa aaactggcca attttgtccg tgaagtgatt ccagagcgtc gtatgcatgc
     9361 caaaggttca ggtgcttttg gtacctttac cgtgaccaat gatattaccc aatatagccg
     9421 cgctaaaatt ttctctgaag tcggcaaaaa aaccgaaatg tttgcgcgtt tctcaacggt
     9481 ggcgggtgaa cgtggtgcgg ccgatgcaga acgtgatatt cgcggttttg ccctgaaatt
     9541 ctatactgaa gaaggcaact gggatctagt tggcaacaat actccggtat ttttcctacg
     9601 ggatgcccgc aagttccctg acctgaataa agcagtcaaa cgtgacccga aaaccaacaa
     9661 gcgcagtgcc accaataact gggatttctg gacattattg cctgaagcct tgcatcaggt
     9721 gaccattgtc atgtcggatc gcggtattcc tgctggctac cgtcatatgc atggttttgg
     9781 cagccatacc ttcagtttta tcaatgccca aaatgaacgc ttctgggtga aattccatat
     9841 gcgtacccag caaggcatcc agaacctgac cgatgctgaa gcagcagact tgattgccaa
     9901 agaccgtgaa agcagccaga ccgacctgtt tgatgccatc gaacgtggcg actatccaaa
     9961 atggaaaatg tacgttcaag tcatgccaga actggaagcc gaaacagtgc cttatcatcc
    10021 atttgacctg accaaagtct ggccgaaggg cgattatcca ctgattgaag tcggtgaatt
    10081 tgagctgaac cgcaatccgg aaaactattt ccaggatgta gaacaggcag cctttgcacc
    10141 tagtaatctt gtaccgggca tcagctactc accggaccgt atgttacagg cacgtttagt
    10201 gaactatgcc gacgcagcgc gttaccgtgt tggtgtaaat cattcacagg ttccggtcaa
    10261 tgctgcacgc tgccctgtaa actctaatcg tcgtgatggt cagggccgca tggatggcaa
    10321 ctatggttca ttgccacatt atgaaccgaa cagttttaac cagtggcagg aacaacctca
    10381 gtttaaagaa ccagcattaa agattaccgg tgatgctgat ttctgggact tccgtgaaga
    10441 tgacaatgat tacttcagcc agccacgtgc cctgttcaat ttgatgaatg atgagcagaa
    10501 acaggccctg tttaataaca cggccgcagc gatgggtgat gcgctagatt tcatcaaata
    10561 ccgtcatatc cgcaactgtt atgcttgcga tccagcttat gggcaaggcg ttgctaaggc
    10621 cttaggtatg actgtagcag atgctcaagc ggcacgtgaa acagatcctg ctaaaggttt
    10681 gccaagtttt ctttaatgtg gaaatttgat ttagatagtt aataaaaaaa cccggcatcc
    10741 tgccagggtt ttttattgga acattaggtc tctgtgctct tgcgtgtctt atatagcgcc
    10801 atcctggtgc ttatttttaa taatatggaa cttcctgttc ctgaccactt aatcatatga
    10861 aaattccact gtcccaggta gccgcaaagg gccgagattt atgtacacca gagcgcacaa
    10921 aatagcctaa atttcatcca taaaaaacat tcaaatttgt atatacaata atgtatcata
    10981 aatttgtatc actcaagcta attggagtaa tttttagtca aaattattgg caagaagtat
    11041 caaacttgct caaaaaaaat ccgctttgtt tccaaagcgg atttttgcgg tagtttcggg
    11101 ttatgtaaaa tagctaagta tatgatttta tttaacttac aaaagacgat ttcgtataac
    11161 gtccattatg ttaaatcagg tttaaaactt aatatcatgt gaattaaagt aggtcggatc
    11221 aaactctatc tctcctattt catcctcgat aatttgcatg taatcgtcat atcttggatg
    11281 tttgggttct tgtaatgcat ctaaaatatc aagatatcca aaaattcctc caatatcttc
    11341 aaatggagca tgattttgac catctataca acatggatat tttttattta aatcaggaga
    11401 ttgtctgctt tctacagtaa tcaaatgttc ccaacaatcg ccaaaatcat atgtatatat
    11461 aattttttta cctacacgta gaacttcttt caatttttgt cttttattga tcttttcatg
    11521 cttaatagtc gtgaattcat gtaaatggct atcttcccaa tcaaaagcag attgaattgc
    11581 cttatggagc ctagataaag tagctgccga tgaaatttga atagttctcc agatttcagg
    11641 atcactgcct agcagactaa tttttaattg aaaaacttca tcagaagatt tgagctttac
    11701 agttcccata acagcctagt tctcaaaaaa tagttttagt aaatctaaaa ttaacataat
    11761 accgcttatt cgaaattcag gctaacgccc ctatacaagg ggacacactt tacacttttt
    11821 taaaacttga tcgcggatct gactttagaa atgaatagat caattgaatt ttggttcttg
    11881 gaaaaatcca gcagcagccc atttacttga gatcggatct ctttaggatc agtcagttct
    11941 ccattcatta agtttttcag tagccctccc actttattca tatctgcttt aaggctacgt
    12001 atctcttgga ttgtttttac atccgtcagg cttttaggct ggtagcccaa accgagatca
    12061 cgcaaatatt gagaagctga taaatttccc tgagcagcat tttcttcaat ttgctttttc
    12121 tcagcttcag ttaccacaac ttgaattact ttttggcgta tccgtttcgg ctttttctgt
    12181 tcagtcattt tttttaattt ccagtacatt ttggctatgc caaaagctcc gcaggacaag
    12241 atgccacgtt gagctgaaag cgaataagta gggtaattta tataaccaag gcttgccatg
    12301 ttatataaca ttacacatct tgccaatatc actgaaagtt caattgcaca taaatgcata
    12361 taattgcaca tcaaaatgaa taaaaaccat ttaaaagctt atagttgcat ataatttcat
    12421 ataaaatctt ataaaagctc ttaattgcat aaatcaaaga aagaggaagt cattatggtc
    12481 accagacatg gcgctaagaa attcatcatc tctgtattac cagaaataga aaaacggctg
    12541 gaagctggtt atacaattaa ggaaatccac gctgatttac ctgagctagc gaacaatatt
    12601 tcttacagtt cagttttacg aaatttgagt aaaatgggaa tcagtgaaaa aaagccacat
    12661 tcaaataaaa tagaaaaatc agtgaacctt gatactgctt ctagttctag ccaaaaaata
    12721 acgaatgctc aaagaaatct tgaaaagcta cagaagcgac ctgagaaatt taaatttgat
    12781 catggaacaa atgataagga attaatctaa gtcctcttta aatatagctc taaatgctgc
    12841 ctgtttgcga tttaagctca aattcacagt gaaaggtaca gaacgagtgt ctacgagtga
    12901 aatcgctgaa attcgcgctt agaccctctg aaaaacagct ttttaggctg tgaagcctaa
    12961 ataagcgtaa atcacatgca aaaaattgag tctagataga gcgcagcgag taaaaaagct
    13021 ctatgagcga agcgaattca tcgtgctttt gctttttatt gaagtcacaa cgagataagc
    13081 caaaaaattg ccccgacgaa tcgagcgaaa gcgagattca atagagtttg agcgtagcga
    13141 aaaccaaggg caatttttct ttgcctgtgc ttttaattat ttttaaagtt tttaaatgct
    13201 tttagacatg ctgaaagcct ttgtttacaa tgctttcaag ggttatatga ctacgtttac
    13261 ctaccaatat gactacgttt acctaccaat atgactacgt ttacctacca atatgactac
    13321 gtttacctac cataggtcta cttatttttt tttgtagatt taactacgtt taccttgcat
    13381 ttaactacat aatattttat atagatttat attcagaaat aaaaaagtag actaataac
//
