LOCUS       NZ_CP103337             3373 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Escherichia coli strain ZY22049 plasmid p8, complete sequence.
ACCESSION   NZ_CP103337
VERSION     NZ_CP103337.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30450095
            Assembly: GCF_028473865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Li,F.
  TITLE     Escherichia coli ZY22049
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2022) Yunnan Tropical and Subtropical Animal Virus
            Disease Laboratory, Yunnan Animal Science and Veterinary Institute,
            Jindian, Panlong District, Kunming, Yunnan 650224, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. JUN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 4790645.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 04:07:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,278
            CDSs (total)                      :: 5,160
            Genes (coding)                    :: 4,871
            CDSs (with protein)               :: 4,871
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 88 of 289
            Pseudo Genes (incomplete)         :: 194 of 289
            Pseudo Genes (internal stop)      :: 47 of 289
            Pseudo Genes (multiple problems)  :: 39 of 289
            Pseudo Genes (short protein)      :: 5 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103337.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZY22049"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="p8"
                     /geo_loc_name="China:Yunnan province"
                     /lat_lon="24.79 N 103.15 E"
                     /collection_date="2022-04-15"
                     /collected_by="Fuxiang Li"
     gene            complement(641..832)
                     /locus_tag="NX000_RS26305"
                     /old_locus_tag="NX000_26240"
     CDS             complement(641..832)
                     /locus_tag="NX000_RS26305"
                     /old_locus_tag="NX000_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            896..1219
                     /locus_tag="NX000_RS26310"
                     /old_locus_tag="NX000_26245"
     CDS             896..1219
                     /locus_tag="NX000_RS26310"
                     /old_locus_tag="NX000_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1428..>1865
                     /locus_tag="NX000_RS26315"
                     /old_locus_tag="NX000_26250"
                     /pseudo=""
     CDS             1428..>1865
                     /locus_tag="NX000_RS26315"
                     /old_locus_tag="NX000_26250"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1897..2457
                     /locus_tag="NX000_RS26320"
                     /old_locus_tag="NX000_26255"
     CDS             1897..2457
                     /locus_tag="NX000_RS26320"
                     /old_locus_tag="NX000_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2464..2697
                     /locus_tag="NX000_RS26325"
                     /old_locus_tag="NX000_26260"
     CDS             2464..2697
                     /locus_tag="NX000_RS26325"
                     /old_locus_tag="NX000_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          477..560
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="95.0"
                     /evalue="4.11e-16"
     Typing          1210..2780
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.628"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagttcccgg tatgtggtgg cgtgacgggt gagatggtgc ggcgaagacg catacgtcac
       61 gcaggcgcgg gttcttccac tgacgcgccc cgtgatcgat ggccttggct ccgtccggca
      121 tatcgagagc ggcaaactac ccgtcccttc actgggaggg gtgagttgtt gccgttccct
      181 ccgaccttcc accatccagc atatcgcacg atcccatgtg caccgctact cctgataact
      241 ccagtgcaaa aaaaaaaacg gatctgcatc acaacgagag tgatcccacc gctcgccgca
      301 gccccagcga ccgaacgtag tgagcgagtg ggcgaggaag cggaagagtc acagaggcaa
      361 gcattgtgca cttacgcgcc tgtgccagtg tccgcgtgtg tgttgtttat cgggcgcgct
      421 gtttttacgc atctgtgcgg ctttttactc ctgacatggt gcacttccca tccactctgc
      481 accgatgcaa aatgaggaaa gccagtatac acatcgctat cgctctgtga actgggtcag
      541 ggctgcgccc cgacacccgc actgacgcgc cctggcgggc ttgtctgctc ccggcatccg
      601 ctcacaggca agctgtgaac gtctccggga gctgcatgtt tcagttgctt tcaccgtcat
      661 cgccgaagcg tgcgaggcaa ctgcggtaga gttcgtcggc gtggtcgtga agtgattcgc
      721 agatgtctgc ctgttcgtcc gcgtccagat cgttgagttt ttccagcagg gttagcgtct
      781 ggtttctgat aaagcgagcc atgttaaggg ctgttttttc ctgtttggtc atggtcttaa
      841 ggctccttgt atgggggatt tgttaggggg aatgataccg atgaaacgcg agaagatgct
      901 cacgatacgg gttaccgatg aggagcacgc ccggttactg gaacgctgcg agggtaaacg
      961 actggcggaa tggatgcggc gggtctgtct gggggagccg gttgcacgca caggaaaact
     1021 gccgacactg gcaccgccat tgctgcggca tctggctgcc atcgggaaca acctgaacca
     1081 gacagcccgg aaggtgaaca gcggccagtg gtcttcgatt gaccgggttc atgtggtggc
     1141 cgcactgatg gccatcgagg gtgaattgcg ccagttgcgg caggctgtca gggaacaggg
     1201 ggtacgggat gatagttaaa tttcatgcca gggggaaagg cggtggaagt ggcccggtcg
     1261 attacctgct ggggcgggag cgtaaccgcg aaggcgcaac ggtgctccgg ggtaacccgg
     1321 aagaagtgcg ggaactcatt gattccacgc cattttcgaa gaaatacacg tcaggcgttc
     1381 tgtcgttcgc ggagaaggag ctaccaccgg gtgaacgcga aagagtgatg acgagctttg
     1441 agcgtgttct tatgcccggt ctcgaaaaag accagtacag cattctctgg gtggaacacc
     1501 aggataaggg gcggctcgaa ctgaattttg tcatcccgaa catggagcta cagagcggta
     1561 agcgcctcca gccgtactac gaccgtgcgg acaggccgag gataaatgcc tggcagacgc
     1621 tggttaatca tcattacggg ctgcgtgacc cgaacgcacc ggagaacaga cggacgcttg
     1681 tcacgccaaa taatctgcca aaagcgaaac aggaggccgc agaagcgatt acacggggtc
     1741 ttgaggctct ttaccatgcc ggagcaataa aaacgcgtca ggatgtcaca gaggcgctta
     1801 cagcggcagg ttttgaggtg gtgaggacaa ccaaaagcag catcagtatt gccgacccgg
     1861 agggggggcg gaatctcagg ctaaaggggg cgctgtatga gcaatctttt acaaatggga
     1921 acagacttcg agaagaaact gaaagagcga gccgcttcta ccgagaacat gctgaacagc
     1981 gagttcagga agctcgaaga gtctgtagac aaggcattga gcttaaacag gcagaaaatc
     2041 agagacgcta tcagcgaaca tacaacatcc gtgaaaaagc aactggatac gctgagcacc
     2101 accgtgagca tgcagttcag caccacagag gccgaactgt cccggcagca gaaaaaactg
     2161 ctctggcggg tgatcaaagg gcggatactg tttccggcgc tgaccgcgct cagcgtcacg
     2221 ggcgggattt tcctggggtg ctgggggctg atggagtggc aggaaagcaa gattgcgaaa
     2281 aatatcctga ctatcagaga gcaggaaaac acgctggcga aactcgaggc aaaaacctgg
     2341 ggggtgacgt tcgtcaatgg cgagaacggg aaatttctgg tgttgccgga cggggtgaaa
     2401 ggggaaaaca cctggacggt gggcgacaag aacgccgtga ggctggtcag ggagtaaacg
     2461 agtatgacgg aactggaagg gcatttgctg aacgccttag agcatttgca acaggactat
     2521 atgcggcggc tgaacgagtg ggagagcgcc ttcgcggaat tgcagaagat gcacgaggtt
     2581 acgcaacgga acaacgcgat actgaacgag cgcgtcgtga tattgagtca gcaggtgcag
     2641 agattagccg ggcaagtgga tcgcttgagt cggttattta tcgcgaacaa cagctaaggg
     2701 atgaacagga gcgtgcccgg tacagacatg aggtacagga acgcgcggca cgggttcgga
     2761 gtgggccatc aatggagatg tgaccggaga agtgacgaaa tcttgccatt ttgacccgtt
     2821 cgacggctgg ggcgagcgaa acaaatcgtg tgccgtattt gtgcatagct atgcaacatc
     2881 ctgaaaacgc atttcacgcg tttttgggga ttcagccagg ggaaaacatg gtttttttac
     2941 tttttgagcg taaagacgtg aaaaatctcg ttattgcact tgcgatcttt cgtcggtggg
     3001 cgtagaacga aaacagctcg ccaggaaggc gagctgtttc gggttcattg aggtctctga
     3061 cctcctcaat gttcctgtgg aaactgttga gatccacaac agaaaccagt tcattcagtt
     3121 tgcatgagcc ttctgtattc ttcaagagtt tttcaacagc acagtctccc aggaaccccg
     3181 aaaaaaacct gtttttgcag atacttgcgt atttgcgaga atcgtttgcg gttgggggag
     3241 ctgtgacgac ggcgaccgca cgcgtgagag ttccgctacc ggcgcagaga gtttcatcga
     3301 cagcggacac ctgatgagtt gccacaaaaa ccgtatgtag cgcggctaga ttggttgagt
     3361 tagaccttgg cgg
//
