LOCUS       NZ_CP103335             6647 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Escherichia coli strain ZY22049 plasmid p6, complete sequence.
ACCESSION   NZ_CP103335
VERSION     NZ_CP103335.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30450095
            Assembly: GCF_028473865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Li,F.
  TITLE     Escherichia coli ZY22049
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2022) Yunnan Tropical and Subtropical Animal Virus
            Disease Laboratory, Yunnan Animal Science and Veterinary Institute,
            Jindian, Panlong District, Kunming, Yunnan 650224, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. JUN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 4790645.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 04:07:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,278
            CDSs (total)                      :: 5,160
            Genes (coding)                    :: 4,871
            CDSs (with protein)               :: 4,871
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 88 of 289
            Pseudo Genes (incomplete)         :: 194 of 289
            Pseudo Genes (internal stop)      :: 47 of 289
            Pseudo Genes (multiple problems)  :: 39 of 289
            Pseudo Genes (short protein)      :: 5 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103335.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZY22049"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="p6"
                     /geo_loc_name="China:Yunnan province"
                     /lat_lon="24.79 N 103.15 E"
                     /collection_date="2022-04-15"
                     /collected_by="Fuxiang Li"
     gene            105..623
                     /locus_tag="NX000_RS26215"
                     /old_locus_tag="NX000_26150"
     CDS             105..623
                     /locus_tag="NX000_RS26215"
                     /old_locus_tag="NX000_26150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            630..863
                     /locus_tag="NX000_RS26220"
                     /old_locus_tag="NX000_26155"
     CDS             630..863
                     /locus_tag="NX000_RS26220"
                     /old_locus_tag="NX000_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1181..1600)
                     /locus_tag="NX000_RS26225"
                     /old_locus_tag="NX000_26160"
     CDS             complement(1181..1600)
                     /locus_tag="NX000_RS26225"
                     /old_locus_tag="NX000_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1681..2118)
                     /locus_tag="NX000_RS26230"
                     /old_locus_tag="NX000_26165"
     CDS             complement(1681..2118)
                     /locus_tag="NX000_RS26230"
                     /old_locus_tag="NX000_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2378..3946
                     /gene="cea"
                     /locus_tag="NX000_RS26235"
                     /old_locus_tag="NX000_26170"
     CDS             2378..3946
                     /gene="cea"
                     /locus_tag="NX000_RS26235"
                     /old_locus_tag="NX000_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(3943..4284)
                     /locus_tag="NX000_RS26240"
                     /old_locus_tag="NX000_26175"
     CDS             complement(3943..4284)
                     /locus_tag="NX000_RS26240"
                     /old_locus_tag="NX000_26175"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            4332..4469
                     /locus_tag="NX000_RS26245"
                     /old_locus_tag="NX000_26180"
     CDS             4332..4469
                     /locus_tag="NX000_RS26245"
                     /old_locus_tag="NX000_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(4668..4826)
                     /locus_tag="NX000_RS26440"
     CDS             complement(4668..4826)
                     /locus_tag="NX000_RS26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5496..5687)
                     /locus_tag="NX000_RS26250"
                     /old_locus_tag="NX000_26185"
     CDS             complement(5496..5687)
                     /locus_tag="NX000_RS26250"
                     /old_locus_tag="NX000_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5736..6074
                     /locus_tag="NX000_RS26255"
                     /old_locus_tag="NX000_26190"
     CDS             5736..6074
                     /locus_tag="NX000_RS26255"
                     /old_locus_tag="NX000_26190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001405931.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVR
                     EQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          5324..5411
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4755..5310)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3..967
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.872"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6065..6645
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.7600000000000003e-124"
ORIGIN
        1 ggcgctcact gaggcggggc tggaagtggt cagggtgacg cgaagcagta tcagcattgc
       61 agatccgaac ggcgggaaga atatcaggct gaaaggagca ttttatgagc aatcttttgc
      121 agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc
      181 tgaacaacga gttcaggagg ctcggcgaat ctgtaagcga ggctgtgaca tcaaacgaga
      241 cgaaaatcag agacgctata gccctgttca cagcctcgac agaggaatcg ctggaaaaac
      301 accgggaagg ggtgaaagag gcgatgatgc agcacaggag ggacgtgtta aagctggcag
      361 ggaatacggg catgatgtta ctggggatag tctttctcct gtttaccgcg agtggcggga
      421 cgctctggta tcttggaggg aggatacagg cgaacctgga agaaatcagg aagcaggaag
      481 agacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag gacgggaaca
      541 ggaaattcct tgtccttccg tacgggaaat cagcggaggt gattcccttt caggggaaag
      601 agtgggtaca tctgaaggag tgacacagag tgacagagct ggaaacacat ttgctgaacg
      661 ccttagagca gctgcaacag gactatatgc agcggctgag cgaatgggag agcgccttcg
      721 tggaattgca gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg aacgagcggg
      781 tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaacgc ttgagccagt
      841 tatacagcga gaactggaga taagagagga acggctgata caggagcgcg aacatgtgtt
      901 atccctggaa cgggagcgtc agccggaaat acaggaacgc acgctggatg gcccttcgct
      961 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
     1021 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg atcgcggcag
     1081 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
     1141 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
     1201 catcgagata aaccgcagaa atcgtgtcag ccagcagcct ggattttttc ggggtaaggt
     1261 ctttgctgcg gtaatcagta cccgcaaatt gagttgtgcc ccggagtgag tttaaaaatt
     1321 cctggctgtg aaactgctga atgcgttgtt gcacatcact ccgtgatttt cctgtcgctt
     1381 tatctttagc tcccatatcc cagaactgcc gaaaaaaagg caccatcatt cgtgttgtgt
     1441 cggctttttg ggtggaaaag ttgggatcaa aaccgtcatc agaagcatat acgtaatgcc
     1501 gggtatgttg ttccacgctg ccctttgggg tacaggctgt gagagctact gttgctgtta
     1561 tcatggcgat aagacaaagt ttgtttccgg tacgtctcat gagtgtctcc ctaccttaaa
     1621 gtatttattc tcggtgagtt attatccgaa gactatttta tcatcagtct ctgacagcgt
     1681 tcagcgaccg aacaacctgg accagaatcc ccgtttcttt tccagttcca gtgcctgcct
     1741 gagctgggct atctcatttt gtagctgttc ccgttctgct tcctggcgtc tgcgatccat
     1801 atcctgtgcc tgtttatcct caagcatcag cgtcaggcat tgtttcagct cattcagctc
     1861 ccggagaatg cgttctgtct gctgatcatg tggattttct gcatgtccct cactgtgcct
     1921 ttctggtgtc tcattctgct ttaattcgcc gtatgcccgg atcagttcac tggtttcaaa
     1981 ctcccgtcgt ccgtccctgc cggtacggta actgacaagg cctgcgcgca tatcacggta
     2041 aaactgtgaa cgcgatctgc ctgtcatttt tagtgcgtcc cggagtgtgt gccatgccat
     2101 aaagtgacag tgtcccatag atgtctcatc tcatagtttc agtaaaacat aatgaggtct
     2161 gagaacggta atgtttgtgc tggtttttgt ggcatcgggc gagaatagcg cgtggtgtga
     2221 aagactgttt ttttgatcgt tttcacaaaa atggaagtcc acagtcttga cagggaaaat
     2281 gcagcggcgt agcttttatg ctgtatataa aaccagtggt tatatgtaca gtatttattt
     2341 ttaacttatt gttttaaaag tcaaagagga ttttataatg gaaaccgcgg tagcgtacta
     2401 taaagatggt gttccttatg atgataaggg acaggtaatt attactcttt tgaatggtac
     2461 tcctgacggg agtggctctg gcggcggagg tggaaaagga ggcagtaaaa gtgaaagttc
     2521 tgcagctatt catgcaactg ctaaatggtc tactgctcaa ttaaagaaaa cacaggcaga
     2581 gcaggctgcc cgggcaaaag ctgcagcgga agcacaggcg aaagcaaagg caaacaggga
     2641 tgcgctgact cagcgcctga aggatatcgt gaatgaggct cttcgtcaca atgcctcacg
     2701 tacgccttca gcaacagagc ttgctcatgc taataatgca gctatgcagg cggaagcaga
     2761 gcgtttgcgc cttgcgaaag cagaagaaaa agcccgtaaa gaagcggaag cagcagaaaa
     2821 ggcttttcag gaagcagaac aacgacgtaa agagattgaa cgggagaagg ctgaaacaga
     2881 acgccagttg aaactggctg aagctgaaga gaaacgactg gctgcattga gtgaagaagc
     2941 taaagctgtt gagatcgccc aaaaaaaact ttctgctgca caatctgaag tggtgaaaat
     3001 ggatggagag attaagactc tcaattctcg tttaagctcc agtatccatg cccgtgatgc
     3061 agaaatgaaa acgctcgctg gaaaacgaaa tgaactggct caggcatccg ctaaatataa
     3121 agaactggat gagctggtca aaaaactatc accaagagcc aatgatccgc ttcagaaccg
     3181 tccttttttt gaagcaacca gacgacgggt tggggccggt aagattagag aagaaaaaca
     3241 aaaacaggta acagcatcag aaacacgtat taaccggata aatgctgata taactcagat
     3301 ccagaaggct atttctcagg tcagtaataa tcgtaatgcc ggtatcgctc gtgttcatga
     3361 agctgaagaa aatttgaaaa aagcacagaa taatctcctt aattcacaga ttaaggatgc
     3421 tgttgatgca acagttagct tttatcaaac gctgactgaa aaatatggtg aaaaatattc
     3481 gaaaatggca caggaacttg ctgataagtc taaaggtaag aaaatcggca atgtgaatga
     3541 agctctcgct gcttttgaaa aatacaagga tgttttaaat aagaaattca gcaaagccga
     3601 tcgtgatgct atttttaatg cgttggcatc ggtgaagtat gatgactggg ctaaacattt
     3661 agatcagttt gccaagtact tgaagattac ggggcatgtt tcttttggat atgatgtggt
     3721 atctgatatc ctaaaaatta aggatacagg tgactggaag ccactatttc ttacattaga
     3781 gaagaaagct gcagatgcag gggtgagtta tgttgttgct ttacttttta gcttgcttgc
     3841 tggaactaca ttaggtattt ggggtattgc tattgttaca ggaattctat gctcctatat
     3901 tgataagaat aaacttaata ctataaatga ggtgttaggg atttaattat tctttattga
     3961 tataaaaagt cctagcaatc caaatgggat tgctaggacc aaacaaagta gattatatag
     4021 cataaatagg tttaattttg ctacgggggc gttatttagg ttttttcttc tttcgaaaaa
     4081 atctttcttt atgaagttaa aagctatgta ttcaatagca tattttgaat atggacatag
     4141 aatagtgctt atcactattg catatagcat cttatctgac acaaggaaat aatacccttc
     4201 gctgtttttt gttataaggt atatatatat aagtgtgcag tacaggccaa ataaaatatt
     4261 ttttatgtag tatcttaagc tcataaatta aacctcgcca tatattcttt tcattttata
     4321 aggatcgagt tatgaggaaa agattttttg tgggaatatt cgcgataaac ctccttgttg
     4381 gatgtcaggc taactatata cgtgatgttc agggagggac catcgcacca tcctcctctt
     4441 ctaaactgac ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt
     4501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccaa cggtggtttg
     4561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4621 gataccaaat actgtccttc tagtgtagcc gtagtcgggc cactacttca agaactctgt
     4681 agcaccgttt gtgccatcat cgctctgcta atccggttac cagtggctgc tgccagtggc
     4741 gttaaggcgt gccttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4801 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4861 ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4921 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4981 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5041 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgcctgcta cgtggccttc
     5101 ttcctgttcc tggtcttttg ctcacatgtt ctttccggcc ttatcccctg attctgtgga
     5161 taactgtgtt accgtttttg tgtgagtcag taccgctcgc cgcagtcgaa cgaccgagcg
     5221 tagcgagtca gtgagcgagg aagcggaaaa gcgcctggac gtgcattttc tccttacgca
     5281 tctgtgcggc atttcacacc cggcatggcg tacttttcat acaatccgca ctgatgccgc
     5341 atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc tgcgccccga
     5401 cacccgctaa aacctgctga cgcgccctga cgggcttgtc agctcccggc atccgctcac
     5461 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg
     5521 aaacgtgcga ggcagctgcg gtaaagctca tcggcgtggt cgtgaagcga ttcacaaata
     5581 tcggcctgtt catctgcgtc cagttcgttg agcttctcca gcagcgttaa tgtctggctt
     5641 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tagtcactga tgcctccgtg
     5701 taagggggat ttctgttcat ggggtaatga taccgatgaa acgcgagagg atgctcacaa
     5761 tacgggttac tgatgatgaa catgcccggt tacttgaacg ctgtgagggt aaacaactgg
     5821 cggtatggat gcggcgggtc tgcctggggg agccggttgc ccgttccgga aaactgccga
     5881 cactggcacc gccgttactg cgtcagctgg ccgccatcgg aaataacctg aatcagacag
     5941 cccgtaaggt gaacagcggg cagtggtctt ccggtgaccg ggttcaggtg gtggccgcac
     6001 tgatggccat cggggatgag ctgcgccggc tgcgtctggc tgtcagggaa cagggggcgc
     6061 gggatgatag ttaaatttca tgccagggga aaaggtggtg gcagtggtcc ggttgattac
     6121 ctgctgggga gggagcgtaa ccgcgaaggc gcaacggtgc ttcagggtaa tccggaagaa
     6181 gtccgggaac tcatcgatgc cacgccattt gcgaagaaat acacgtccgg tgttctgtcg
     6241 ttcgcggaga aggagctgcc gccgggagga cgtgaaaaag tgatggcgag ctttgagcgt
     6301 gtactgatgc ccggtctcga aaaaaatcag tacagcatcc tgtgggtgga gcaccaggac
     6361 aagggacggc ttgagctgaa ttttgtcatt ccgaacatgg agctacagac cggaaaacgc
     6421 ctccagccgt actacgaccg cgcagacagg cctagaattg atgcctggca gacgctggtg
     6481 aatcaccatt acgggctgca tgacccgaac gccccggaga accgcaggac gctgacactc
     6541 cctgataacc tgcctgaaac gaaacaggcg cttgctgagg gcgtcacgcg aggtatagat
     6601 gcactttacc atgccggaga gataaaaggc cgtcaggatg tgattca
//
