LOCUS       NZ_CP103334             8786 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Escherichia coli strain ZY22049 plasmid p5, complete sequence.
ACCESSION   NZ_CP103334
VERSION     NZ_CP103334.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30450095
            Assembly: GCF_028473865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Li,F.
  TITLE     Escherichia coli ZY22049
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2022) Yunnan Tropical and Subtropical Animal Virus
            Disease Laboratory, Yunnan Animal Science and Veterinary Institute,
            Jindian, Panlong District, Kunming, Yunnan 650224, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. JUN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 4790645.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 04:07:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,278
            CDSs (total)                      :: 5,160
            Genes (coding)                    :: 4,871
            CDSs (with protein)               :: 4,871
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 88 of 289
            Pseudo Genes (incomplete)         :: 194 of 289
            Pseudo Genes (internal stop)      :: 47 of 289
            Pseudo Genes (multiple problems)  :: 39 of 289
            Pseudo Genes (short protein)      :: 5 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103334.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZY22049"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="p5"
                     /geo_loc_name="China:Yunnan province"
                     /lat_lon="24.79 N 103.15 E"
                     /collection_date="2022-04-15"
                     /collected_by="Fuxiang Li"
     gene            456..778
                     /locus_tag="NX000_RS26160"
                     /old_locus_tag="NX000_26095"
                     /pseudo=""
     CDS             456..778
                     /locus_tag="NX000_RS26160"
                     /old_locus_tag="NX000_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            768..>1568
                     /locus_tag="NX000_RS26165"
                     /old_locus_tag="NX000_26100"
                     /pseudo=""
     CDS             768..>1568
                     /locus_tag="NX000_RS26165"
                     /old_locus_tag="NX000_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1456..1863
                     /locus_tag="NX000_RS26170"
                     /old_locus_tag="NX000_26105"
     CDS             1456..1863
                     /locus_tag="NX000_RS26170"
                     /old_locus_tag="NX000_26105"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_241525192.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTALSVTGGIFLGCWGL
                     IQWQESRIAKNILTIREQENTLAKLEAKTWG"
                     /name="MbeB family mobilization protein"
     gene            1980..2213
                     /locus_tag="NX000_RS26175"
                     /old_locus_tag="NX000_26110"
     CDS             1980..2213
                     /locus_tag="NX000_RS26175"
                     /old_locus_tag="NX000_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2900..3739)
                     /locus_tag="NX000_RS26180"
                     /old_locus_tag="NX000_26115"
     CDS             complement(2900..3739)
                     /locus_tag="NX000_RS26180"
                     /old_locus_tag="NX000_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272592201.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKHLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(4747..4938)
                     /locus_tag="NX000_RS26185"
                     /old_locus_tag="NX000_26120"
     CDS             complement(4747..4938)
                     /locus_tag="NX000_RS26185"
                     /old_locus_tag="NX000_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001677262.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5001..5324
                     /locus_tag="NX000_RS26190"
                     /old_locus_tag="NX000_26125"
     CDS             5001..5324
                     /locus_tag="NX000_RS26190"
                     /old_locus_tag="NX000_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5314..>6155
                     /locus_tag="NX000_RS26195"
                     /old_locus_tag="NX000_26130"
                     /pseudo=""
     CDS             5314..>6155
                     /locus_tag="NX000_RS26195"
                     /old_locus_tag="NX000_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6043..6450
                     /locus_tag="NX000_RS26200"
                     /old_locus_tag="NX000_26135"
     CDS             6043..6450
                     /locus_tag="NX000_RS26200"
                     /old_locus_tag="NX000_26135"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_241525192.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTALSVTGGIFLGCWGL
                     IQWQESRIAKNILTIREQENTLAKLEAKTWG"
                     /name="MbeB family mobilization protein"
     gene            6567..6800
                     /locus_tag="NX000_RS26205"
                     /old_locus_tag="NX000_26140"
     CDS             6567..6800
                     /locus_tag="NX000_RS26205"
                     /old_locus_tag="NX000_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7488..8327)
                     /locus_tag="NX000_RS26210"
                     /old_locus_tag="NX000_26145"
     CDS             complement(7488..8327)
                     /locus_tag="NX000_RS26210"
                     /old_locus_tag="NX000_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272592201.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKHLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(8519..8668)
                     /locus_tag="NX000_RS26435"
     CDS             complement(8519..8668)
                     /locus_tag="NX000_RS26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     Typing          4575..4659
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(2..256)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.31"
                     /coverage="100.0"
                     /evalue="5.52e-88"
     Typing          3739..4189
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.779"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          8327..8778
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          424..554
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.091"
                     /coverage="100.0"
                     /evalue="1.05e-44"
     Typing          769..1094
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.578"
                     /coverage="83.0"
                     /evalue="1.72e-61"
     Typing          1375..1859
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.383"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1857..2311
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5315..5973
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6444..6898
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
       61 cctgtcgggt ttcgccacct ctgacttgag catctgtttt tgtgatgctc gtcagggggc
      121 ggagcctatg gaaacgtgcc agcggcgctg ggcctcttcc tgttcctggc ctttgctcac
      181 atgtttctct tttggcgtta tcccctgatt ctgtggataa ccgcatcacc gtctttgagt
      241 gagttgatac cgctcgtagc gagtcagtga gcgaggaagt aaagagcgcc tgatgcggca
      301 ttttccttac gtgcggtatt tcacaccgca tatggtgcac ttttctttat aatccgcact
      361 gatgccacat agttaagtta gtatacactc cgccatcgct acgtgactgg tcagggggat
      421 ttttaggggg aatgataccg atgaaacgcg agaagatgct cacaatacgg gttagcgatg
      481 aggagcacgc ccggttactg gaacgctgcg agggtaaacg actggcagaa tggatgcggc
      541 gggtctgtct ggggagccgg ttgcgcgcac aggaaaactg ccgacactat cgccgccatt
      601 gctgcggcat ctggctgcca tcgggaacaa tctgaaccag acagcccgga aggtgaacag
      661 cggccactgg tcttcgattg accgggttca tgtggtggcc gctctgatgg ccatcgaggg
      721 tgaattgcgc cagttgcggc aggctgtcag ggaacagggg ggacgggatg atagttaaat
      781 ttcatgccag ggggaaaggc ggtggaagtg gcccggtcga ttacctgctg gggcgggagc
      841 gtaaccgcga aggcgcaacg gtgctccggg gtaacccgga agaaatccgg gaactcattg
      901 atgccacgcc attttcgaag aaatacacgt caggcgttct gtcgttcgcg gagaaggagt
      961 taccgccggg tgaacgcgaa agagtgatga cgagctttga gcgtgttctt atgcccggtc
     1021 tcgaaaaaga ccagtacagc attctctggg tggaacacca ggataagggg cggctcgaac
     1081 tgaattttgt catactacga ccgtgcggac aggccgagga taaatgcctg gcagacgctg
     1141 gttaatcatc attacgggct gcgtgacccg aacgcaccgg agaacagacg gacgcttgtc
     1201 acaccgaata atctgccaaa agcgaaacag gaggccgcag aagcgattac acggggtctt
     1261 gaggcgcttt accgtgccgg agcaataaaa acgcgtcagg atgtcactga ggcgctcaca
     1321 gcggcaggtt ttgaggtggt gaggacaacc agaagcagta tcagtattgc cgacccggag
     1381 gggggcggaa tctcaggcta aagggggcgc tgtatgagca atcttttaca aatgggaacg
     1441 gacttcgaga agaaactgaa agagcgagcc gcttctaccg agaacatgct gaacagcgag
     1501 ttcaggaagc tagaagagtc tgtagacaag gcactgagct taaacaggca gaaaatcaga
     1561 gacgctatca gcgaacatac aacatccgtg aaacagcaac tggatacgct gagcaccacc
     1621 gtgagcacgc agctcagcac cacagaggcc gaactgtccc ggcagcagaa aaacctgctc
     1681 tggcaggtga tcaaagggcg ggtactgttt ccggcgctga ccgcgctcag cgtcacgggc
     1741 ggcattttcc tggggtgctg ggggctgatt cagtggcagg aaagcaggat tgcgaaaaat
     1801 atcttgacca tcagagagca ggaaaacacg ctggcgaaac tcgaggcaaa aacctggggg
     1861 tgacgttcgt caatggcgag aacgggaaat ttctggtgct gccggacggg gtgaaagggg
     1921 aaaacacctg gacggtgggc gacaagaacg ccgtgaggct ggtcagggag taaacgagta
     1981 tgacggaact ggaagggcat ttgctgaacg ccttagagca tttgcaacag gactatatgc
     2041 ggcggctgaa cgagtgggag agcgccttcg cagaattgca gaagatgcac gcggttacgc
     2101 aacagaacaa cgcgatactg aacgagcgtg tcgtgaactt gagtcagcag gtgcagctat
     2161 tagccggaca agtcgaccgc ttgagtcggt tatttatcac gaacaacaga taagggatga
     2221 acaggagcgt caggcgcagg agcttgcccg gcgctggcat gaagagcagg aacccgaagc
     2281 gcggatctgg cgtggcccgt caatggggat gtgaccgggg taccccctga aatcgagcca
     2341 gtttgtggca cgccctaccc ggatggcgcc aatgttaatg tacgtacatt agcattaaca
     2401 atatccaaac ctccccattt tgccccgttc gacggctgga acgtgcgaaa ctgatcgtca
     2461 ttcagtttcg cgcataacta tgcatgaggt taaaatttac caggcgcgat cgcggcagtt
     2521 tttcgggtgg tttgttgctg tttttacctg tctgctgccg tgatcgcgct gaacgcgttt
     2581 aagcggtacg cgcaatgcga cgttatggta aatacggagt attttaattg cgggcctttg
     2641 ttgtatatct ctgtcgtttg tccccggggg aagcattact gctaacaagt cccgtgagaa
     2701 aactgcgcaa tcgtattgcg caccatgaac ctattctcaa ccgcaatctg gaggatgatt
     2761 ttgctacgat aaagagaatt atcgcgtatc ggtgtcagca ttcattggag tggatgctga
     2821 aaaaccaggt gttactccct ctacttacac ttaaacctct ctaaaaatat aagtaagtac
     2881 tatcgagggc tatcatgaac tatggtgccc tctgagcata gctacccatt acttcttcta
     2941 tgtcattgaa taattcattt cttaactcta agtattctag taactccatt tctaatgact
     3001 ctgtcgtgaa cttaaatcta gaaggatctt tgattgagtt tactagtctt gataacgctc
     3061 tatatgtttt gagtgaaggt tgcattatag atggaaagta tttgaaaacc tcttcatctc
     3121 tgttgaaatt aactaaaaac ttgtagtcat catttataaa tctcaagcat acagcgtcaa
     3181 gcttatcgcc ttttcttatt attatttctt tataggaaag atgtttttca gaagataagt
     3241 cccacatcaa gcgaaaatct ctctttgcga acgagttcgc gtcatcattg aaaagagatg
     3301 atgtgatggc tttggataga ccttctgtgt ttatctcttt gttggatggg tagtaaattt
     3361 tattaattgc tttcattctc tcaaatgatg aatataggtc attatgccac cttgttgaaa
     3421 tcactccttt agttatattc tccttgctaa aattaagccc tgtatcaaaa ataaatgcag
     3481 ccatcagtga atatgatatg ttttttatta actcagactt aaatggttcc gctatggagt
     3541 agtacagtga taaaataact aaaaataaaa ggtattgtct tacctttccc gatgagtata
     3601 tgtaaaggcg atatttattt attgatagtt ttattttgta tggaatgatc tcatttctgt
     3661 catatcctct tcgtggtgga gcaaaacaaa agaaacatgc agctattaat aaaacaaaaa
     3721 agatgaacag tgtaagcaag ttcggctcct gtgtatgctc actatgcatg attgtcttga
     3781 gatctaacga tcctgaaggt agtcttgtcc gtaaacgaaa aaaccgccca gcaaggcggc
     3841 ttttcgaagg ttctcagagc agcaactctt tgaaccaagg taactggctt ggaggagcac
     3901 agccaccaaa tctgtccttt cagtttagcc ttaaccggcg cataacttca agactaactc
     3961 ctctaaacgg ttaccaatgg ctgctgccag tggcgttttt acgtgtcttt ccgggttgga
     4021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg ttcgtgcaca
     4081 cagcccagct tggagcgaac gacctacacc gggccgagat accaacagcg tgagctatga
     4141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4201 ggaacaggag agcgcacgag ggagcttcca ggggaaacgc ctggtatctt tatagtcctg
     4261 tcgggtttcg ccacctctga cttgagcatc tgtttttgtg atgctcgtca gggggcggag
     4321 cctatggaaa aacgccagcg gcgcggcctt cttcctgttc ctggcctttt gctcacatgt
     4381 ttcttttggc gttatcccct gattctgtgg ataaccgcat caccgtcttt gagtgagctg
     4441 ataccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag
     4501 agcgcctgat gcggcatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     4561 gcacttttct tataatccgc actgatgcca catagttaag ccagtataca ctccgctatc
     4621 gctacgtgac tgggtcaggg ctgcgccccg acacccgcta aaacccgctg acgcgccctg
     4681 acgggcttgt ctgctcccgg catccgctca caggcaagct gtgaacgtct ccgggagctg
     4741 catgtttcag ttgctttcac cgtcatcgcc gaagcgtgcg aggcaactac ggtagagttc
     4801 gtcggcgtgg tcgtgaagtg attcgcagat gtctgcctgt tcgtccgcgt ccagctcgtt
     4861 gagtttttcc agcagggtca gcgtctggct tctgataaag cgggccatgt taatggctgt
     4921 tttttcctgt ttggtcatgg tcttaaggct ccttaatagg ggatttttta gggggaatga
     4981 taccgatgaa acgcgagaag atgctcacaa tacgggttag cgatgaggag cacgcccggt
     5041 tactggaacg ctgcgagggt aaacgactgg cagaatggat gcggcgggtc tgtctggggg
     5101 agccggttgc gcgcacagga aaactgccga cactatcgcc gccattgctg cggcatctgg
     5161 ctgccatcgg gaacaatctg aaccagacag cccggaaggt gaacagcggc cactggtctt
     5221 cgattgaccg ggttcatgtg gtggccgctc tgatggccat cgagggtgaa ttgcgccagt
     5281 tgcggcaggc tgtcagggaa caggggggac gggatgatag ttaaatttca tgccaggggg
     5341 aaaggcggtg gaagtggccc ggtcgattac ctgctggggc gggagcgtaa ccgcgaaggc
     5401 gcaacggtgc tccggggtaa cccggaagaa atccgggaac tcattgatgc cacgccattt
     5461 tcgaagaaat acacgtcagg cgttctgtcg ttcgcggaga aggagttacc gccgggtgaa
     5521 cgcgaaagag tgatgacgag ctttgagcgt gttcttatgc ccggtctcga aaaagaccag
     5581 tacagcattc tctgggtgga acaccaggat aaggggcggc tcgaactgaa ttttgtcatt
     5641 ccgaacatgg aactacagag cggtaagcgc ctccagccgt actacgaccg tgcggacagg
     5701 ccgaggataa atgcctggca gacgctggtt aatcatcatt acgggctgcg tgacccgaac
     5761 gcaccggaga acagacggac gcttgtcaca ccgaataatc tgccaaaagc gaaacaggag
     5821 gccgcagaag cgattacacg gggtcttgag gcgctttacc gtgccggagc aataaaaacg
     5881 cgtcaggatg tcactgaggc gctcacagcg gcaggttttg aggtggtgag gacaaccaga
     5941 agcagtatca gtattgccga cccggagggg ggcggaatct caggctaaag ggggcgctgt
     6001 atgagcaatc ttttacaaat gggaacggac ttcgagaaga aactgaaaga gcgagccgct
     6061 tctaccgaga acatgctgaa cagcgagttc aggaagctag aagagtctgt agacaaggca
     6121 ctgagcttaa acaggcagaa aatcagagac gctatcagcg aacatacaac atccgtgaaa
     6181 cagcaactgg atacgctgag caccaccgtg agcacgcagc tcagcaccac agaggccgaa
     6241 ctgtcccggc agcagaaaaa cctgctctgg caggtgatca aagggcgggt actgtttccg
     6301 gcgctgaccg cgctcagcgt cacgggcggc attttcctgg ggtgctgggg gctgattcag
     6361 tggcaggaaa gcaggattgc gaaaaatatc ttgaccatca gagagcagga aaacacgctg
     6421 gcgaaactcg aggcaaaaac ctgggggtga cgttcgtcaa tggcgagaac gggaaatttc
     6481 tggtgctgcc ggacggggtg aaaggggaaa acacctggac ggtgggcgac aagaacgccg
     6541 tgaggctggt cagggagtaa acgagtatga cggaactgga agggcatttg ctgaacgcct
     6601 tagagcattt gcaacaggac tatatgcggc ggctgaacga gtgggagagc gccttcgcag
     6661 aattgcagaa gatgcacgcg gttacgcaac agaacaacgc gatactgaac gagcgtgtcg
     6721 tgaacttgag tcagcaggtg cagctattag ccggacaagt cgaccgcttg agtcggttat
     6781 ttatcacgaa caacagataa gggatgaaca ggagcgtcag gcgcaggagc ttgcccggcg
     6841 ctggcatgaa gagcaggaac ccgaagcgcg gatctggcgt ggcccgtcaa tggggatgtg
     6901 accggggtac cccctgaaat cgagccagtt tgtggcacgc cctacccgga tggcgccaat
     6961 gttaatgtac gtacattagc attaacaata tccaaacctc cccattttgc cccgttcgac
     7021 ggctggaacg tgcgaaactg atcgtcattc agtttcgcgc ataactatgc atgaggttaa
     7081 aatttaccag gcgcgatcgc ggcagttttt cgggtggttt gttgctgttt ttacctgtct
     7141 gctgccgtga tcgcgctgaa cgcgtttaag cggtacgcgc aatgcgacgt tatggtaaat
     7201 acggagtatt ttaattgcgg gcctttgttg tatatctctg tcgtttgtcc ccggggggaa
     7261 gcattactgc taacaagtcc cgtgagaaaa ctgcgcaatc gtattgcgca ccatgaacct
     7321 attctcaacc gcaatctgga ggatgatttt gctacgataa agagaattat cgcgtatcgg
     7381 tgtcagcatt cattggagtg gatgctgaaa aaccaggtgt tactccctct acttacactt
     7441 aaacctctct aaaaatataa gtaagtacta tcgagggcta tcatgaacta tggtgccctc
     7501 tgagcatagc tacccattac ttcttctatg tcattgaata attcatttct taactctaag
     7561 tattctagta actccatttc taatgactct gtcgtgaact taaatctaga aggatctttg
     7621 attgagttta ctagtcttga taacgctcta tatgttttga gtgaaggttg cattatagat
     7681 ggaaagtatt tgaaaacctc ttcatctctg ttgaaattaa ctaaaaactt gtagtcatca
     7741 tttataaatc tcaagcatac agcgtcaagc ttatcgcctt ttcttattat tatttcttta
     7801 taggaaagat gtttttcaga agataagtcc cacatcaagc gaaaatctct ctttgcgaac
     7861 gagttcgcgt catcattgaa aagagatgat gtgatggctt tggatagacc ttctgtgttt
     7921 atctctttgt tggatgggta gtaaatttta ttaattgctt tcattctctc aaatgatgaa
     7981 tataggtcat tatgccacct tgttgaaatc actcctttag ttatattctc cttgctaaaa
     8041 ttaagccctg tatcaaaaat aaatgcagcc atcagtgaat atgatatgtt ttttattaac
     8101 tcagacttaa atggttccgc tatggagtag tacagtgata aaataactaa aaataaaagg
     8161 tattgtctta cctttcccga tgagtatatg taaaggcgat atttatttat tgatagtttt
     8221 attttgtatg gaatgatctc atttctgtca tatcctcttc gtggtggagc aaaacaaaag
     8281 aaacatgcag ctattaataa aacaaaaaag atgaacagtg taagcaagtt cggctcctgt
     8341 gtatgctcac tatgcatgat tgtcttgaga tctaacgatc ctgaaggtag tcttgtccgt
     8401 aaacgaaaaa accgcccagc aaggcggctt ttcgaaggtt ctcagagcag caactctttg
     8461 aaccaaggta actggcttgg aggagcacag ccaccaaatc tgtcctttca gtttagcctt
     8521 aaccggcgca taacttcaag actaactcct ctaaacggtt accaatggct gctgccagtg
     8581 gcgtttttac gtgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     8641 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8701 ggccgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8761 cggacaggta tccggtaagc ggcagg
//
