LOCUS       NZ_CP103333            14096 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Escherichia coli strain ZY22049 plasmid p4, complete sequence.
ACCESSION   NZ_CP103333
VERSION     NZ_CP103333.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30450095
            Assembly: GCF_028473865.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14096)
  AUTHORS   Li,F.
  TITLE     Escherichia coli ZY22049
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14096)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2022) Yunnan Tropical and Subtropical Animal Virus
            Disease Laboratory, Yunnan Animal Science and Veterinary Institute,
            Jindian, Panlong District, Kunming, Yunnan 650224, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. JUN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 4790645.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 04:07:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,278
            CDSs (total)                      :: 5,160
            Genes (coding)                    :: 4,871
            CDSs (with protein)               :: 4,871
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 88 of 289
            Pseudo Genes (incomplete)         :: 194 of 289
            Pseudo Genes (internal stop)      :: 47 of 289
            Pseudo Genes (multiple problems)  :: 39 of 289
            Pseudo Genes (short protein)      :: 5 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103333.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZY22049"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="p4"
                     /geo_loc_name="China:Yunnan province"
                     /lat_lon="24.79 N 103.15 E"
                     /collection_date="2022-04-15"
                     /collected_by="Fuxiang Li"
     gene            1..858
                     /gene="repA"
                     /locus_tag="NX000_RS26050"
                     /old_locus_tag="NX000_25980"
     CDS             1..858
                     /gene="repA"
                     /locus_tag="NX000_RS26050"
                     /old_locus_tag="NX000_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_032495102.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESDAGVLSITRATRALTSLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDVSEV
                     AVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDADRERQDIITLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            1797..2450
                     /locus_tag="NX000_RS26055"
                     /old_locus_tag="NX000_25985"
     CDS             1797..2450
                     /locus_tag="NX000_RS26055"
                     /old_locus_tag="NX000_25985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000616807.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLFF
                     SHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALALPA
                     EPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGALITS
                     LLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
                     /name="CPBP family intramembrane glutamic endopeptidase"
     gene            2543..2800
                     /gene="pemI"
                     /locus_tag="NX000_RS26060"
                     /old_locus_tag="NX000_25990"
     CDS             2543..2800
                     /gene="pemI"
                     /locus_tag="NX000_RS26060"
                     /old_locus_tag="NX000_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="WP_000557619.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
                     /name="pemI"
     gene            2733..3134
                     /gene="pemK"
                     /locus_tag="NX000_RS26065"
                     /old_locus_tag="NX000_25995"
     CDS             2733..3134
                     /gene="pemK"
                     /locus_tag="NX000_RS26065"
                     /old_locus_tag="NX000_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_001398199.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGT
                     RPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTID
                     MKARGGKRLERVPETIMNEVLGRLSTILT"
                     /name="pemK"
     gene            3383..>3798
                     /locus_tag="NX000_RS26070"
                     /old_locus_tag="NX000_26000"
                     /pseudo=""
     CDS             3383..>3798
                     /locus_tag="NX000_RS26070"
                     /old_locus_tag="NX000_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<3867..>4379)
                     /locus_tag="NX000_RS26075"
                     /old_locus_tag="NX000_26005"
                     /pseudo=""
     CDS             complement(<3867..>4379)
                     /locus_tag="NX000_RS26075"
                     /old_locus_tag="NX000_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            complement(4445..5149)
                     /locus_tag="NX000_RS26080"
                     /old_locus_tag="NX000_26010"
     CDS             complement(4445..5149)
                     /locus_tag="NX000_RS26080"
                     /old_locus_tag="NX000_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <5215..5280
                     /locus_tag="NX000_RS26085"
                     /old_locus_tag="NX000_26015"
                     /pseudo=""
     CDS             <5215..5280
                     /locus_tag="NX000_RS26085"
                     /old_locus_tag="NX000_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            6729..6963
                     /locus_tag="NX000_RS26090"
                     /old_locus_tag="NX000_26020"
                     /pseudo=""
     CDS             6729..6963
                     /locus_tag="NX000_RS26090"
                     /old_locus_tag="NX000_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382250.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7091..>7294
                     /locus_tag="NX000_RS26095"
                     /old_locus_tag="NX000_26025"
                     /pseudo=""
     CDS             7091..>7294
                     /locus_tag="NX000_RS26095"
                     /old_locus_tag="NX000_26025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(7495..7845)
                     /locus_tag="NX000_RS26100"
                     /old_locus_tag="NX000_26030"
     CDS             complement(7495..7845)
                     /locus_tag="NX000_RS26100"
                     /old_locus_tag="NX000_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="WP_000361389.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTAG
                     LALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQKSD
                     EETKKNAMKLIN"
                     /name="plasmid partitioning/stability family protein"
     gene            complement(7848..8810)
                     /gene="parM"
                     /locus_tag="NX000_RS26105"
                     /old_locus_tag="NX000_26035"
                     /gene_synonym="stbA"
     CDS             complement(7848..8810)
                     /gene="parM"
                     /locus_tag="NX000_RS26105"
                     /old_locus_tag="NX000_26035"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="WP_000959884.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKKV
                     FNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTLPL
                     TEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQELDEL
                     DSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGSSYLA
                     DDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHVMVIGG
                     GAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
                     /name="parM"
     gene            complement(8916..9122)
                     /locus_tag="NX000_RS26430"
     CDS             complement(8916..9122)
                     /locus_tag="NX000_RS26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306173356.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRSCRAASAGAVKRGARRHL
                     LLPAKQNPKTTHTQPNKTPKQDK"
                     /name="hypothetical protein"
     gene            9023..9250
                     /locus_tag="NX000_RS26110"
                     /old_locus_tag="NX000_26040"
     CDS             9023..9250
                     /locus_tag="NX000_RS26110"
                     /old_locus_tag="NX000_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_170942586.1"
                     /translation="MTRRGRNAACGQCPASGRARDGRFYRSRLALVTVYASPSPFSDER
                     PSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
                     /name="hypothetical protein"
     gene            9327..10010
                     /locus_tag="NX000_RS26115"
                     /old_locus_tag="NX000_26045"
     CDS             9327..10010
                     /locus_tag="NX000_RS26115"
                     /old_locus_tag="NX000_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="WP_000085883.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAGD
                     KTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYTSK
                     AAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESFTHP
                     NAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAANDDWF
                     MPEAA"
                     /name="DNA methylase"
     gene            10011..10232
                     /locus_tag="NX000_RS26120"
                     /old_locus_tag="NX000_26050"
     CDS             10011..10232
                     /locus_tag="NX000_RS26120"
                     /old_locus_tag="NX000_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001104881.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPERL
                     VRQTLFRANRLLMEAYTEILELEACFKD"
                     /name="hypothetical protein"
     gene            10246..10680
                     /locus_tag="NX000_RS26125"
                     /old_locus_tag="NX000_26055"
     CDS             10246..10680
                     /locus_tag="NX000_RS26125"
                     /old_locus_tag="NX000_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="WP_000274503.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLTG
                     HEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQAL
                     WKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
                     /name="DUF1380 family protein"
     gene            <10726..11088
                     /locus_tag="NX000_RS26130"
                     /old_locus_tag="NX000_26060"
                     /pseudo=""
     CDS             <10726..11088
                     /locus_tag="NX000_RS26130"
                     /old_locus_tag="NX000_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /name="fertility inhibition protein FinO"
     gene            11220..11420
                     /locus_tag="NX000_RS26135"
                     /old_locus_tag="NX000_26065"
     CDS             11220..11420
                     /locus_tag="NX000_RS26135"
                     /old_locus_tag="NX000_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059022.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAARV
                     APRTDWQQFARQRAERCQRRR"
                     /name="hypothetical protein"
     gene            11806..12405
                     /locus_tag="NX000_RS26140"
                     /old_locus_tag="NX000_26070"
     CDS             11806..12405
                     /locus_tag="NX000_RS26140"
                     /old_locus_tag="NX000_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="WP_105906770.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNNV
                     VLPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYDGI
                     VRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTFNNY
                     LKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
                     /name="PIN domain-containing protein"
     gene            12467..12799
                     /locus_tag="NX000_RS26145"
                     /old_locus_tag="NX000_26075"
     CDS             12467..12799
                     /locus_tag="NX000_RS26145"
                     /old_locus_tag="NX000_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152916585.1"
                     /translation="MAFISMTRQKAPYRYASKTRLSIRRTDSGSAGCYVTVAVRGKQEF
                     IIAELDSEIRKIRLKLTDSYEEGVRLSSGTFTLPARFCREILPDDVRSITIILEKSDDE
                     WWYGSY"
                     /name="hypothetical protein"
     gene            13104..13253
                     /locus_tag="NX000_RS26150"
                     /old_locus_tag="NX000_26080"
     CDS             13104..13253
                     /locus_tag="NX000_RS26150"
                     /old_locus_tag="NX000_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001312851.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAY
                     EARK"
                     /name="Hok/Gef family protein"
     gene            13537..13785
                     /locus_tag="NX000_RS26155"
                     /old_locus_tag="NX000_26085"
     CDS             13537..13785
                     /locus_tag="NX000_RS26155"
                     /old_locus_tag="NX000_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083837.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKELR
                     VFIPAALKAQLQEMCDAEGVTQAEMIAELIKQKSAFS"
                     /name="replication regulatory protein RepA"
     Typing          104..858
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(13841..14096)
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.05e-131"
ORIGIN
        1 gtgactgatc ttcaacaaac ttattaccgc caggtaaaga acccgaatcc ggtgtttaca
       61 ccccgtgaag gtgccggaac gctgaagttt tgcgaaaaac tgatggaaaa ggcggtaggc
      121 ttcacctccc gttttgattt cgccattcat gtggcacatg cccgttcgcg tggtctgcgt
      181 cggcgcatgc caccggtact gcgtcgtcgg gctattgatg cgctgctgca ggggctctgt
      241 tttcactatg atccgctggc caaccgtgtc cagtgctcca tcaccacgct ggccattgag
      301 tgtggactgg cgacggagtc tgatgccgga gtactctcca tcacccgtgc cacccgtgcc
      361 ctgacgtccc tggctgagct gggtctgatt acctaccaga cggaatatga cccgcttatt
      421 ggttgcaaca ttccgaccga tatcacgttc acaccggcgc tgtttgccgc tctcgatgtg
      481 tctgaggtgg cggtggcttc cgcacgccgc agccgtgtgg aatgggaaaa cagacaacgc
      541 aaaaagcagg ggctggatac cctgggcatg gatgaactga tagcgaaagc ctggcgtttt
      601 gttcgtgagc gtttccgcag ttaccagaca gagcttaagt cccggggaat aaagcgtgcc
      661 cgtgcgcgtc gtgatgcgga cagggaacgt caggatatta tcaccctggt gaaacggcag
      721 ctgacgcgcg aaatcgcgga agggcgcttc actgccagtc gtgaggcggt aaaacgtgaa
      781 gtggagcgtc gtgtgaagga gcgcatgatt ctgtcacgta accgcaatta cagccggctg
      841 gccacagctt ccccctgaaa gtgacctctt ctgaataatc cggcccgaac cggaggcatt
      901 cgcacgcctg aagcctgcca gcgaacaaaa aatcagcacc acatacaaaa aacaacctca
      961 ctatccacct tctggagcat ccggttcccc ctgtttttaa tacaaaatgc gcctcagcga
     1021 cggggaattt tgcttatcca catttaactg caagggactt ccccataagg ttacaaccgt
     1081 tcatgtcata aagcgccagc cgccagtctt acagggtgca atgtatcttt taaacacctg
     1141 tttatatctc ctttaaacta cttaattaca ttcatttaaa aagaaaacct attcactgcc
     1201 tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc gaccggagcc
     1261 actttagtta caacacacaa aaacaacctc cagaaaaacc ccggtccagc gcagaaccga
     1321 aaccacaaag cccctccctc ataactgaaa agcggccccg ccccggccca aagggccgga
     1381 acagagtcgc ttttaattat gaatgttgta actacatctt catcgctgtc agtcttctcg
     1441 ctggaagttc tcagtacacg ctcgtaagcg gccctcacgg cccgctaacg cggagatacg
     1501 ccccgacttc gggtaaaccc tcgtcgggac cactccgacc gcgcacagaa gctctctcat
     1561 ggctgaaagc gggtatggtc tggcagggct ggggatgggt aaggtgaaat ctatcaatca
     1621 gtaccggctt acgccgggct tcggcggttt tactcctgta tcatatgaaa caacagagtg
     1681 ccgccttcca tgccgctgat gcggcatatc ctggtaacga tatctgaatt gttatacatg
     1741 tgtatatacg tggtaatgac aaaaatagga caagttaaaa atttacaggc gatgcaatga
     1801 ttcaaacacg taatcaatat ctgcagttta tgctggttat gctggctgca tggggcatta
     1861 gttggggagc cagatttgtc atggagcagg ccgttctgct ttatggatca ggaaaaaact
     1921 atttgttctt cagtcatggt actgttctga tgtacctgct gtgtgttttc ctggtatacc
     1981 gccgttggat agctccgcta ccggtcgttg gtcagctgcg caacgttggc gtaccgtggc
     2041 tggtcggtgc gatggccgtg gtgtatgtcg gtgtatttct gctcggtaag gcgctggctc
     2101 tgcctgctga gccatttatg acgaaacttt ttgccgataa gtccatacct gacgtgatcc
     2161 tgacgttgct gaccatcttt atccttgccc cgttgaatga ggaaacgctg ttccggggga
     2221 ttatgctgaa cgtcttccgt tcacggtact gctggacgat gtggctgggg gcgctgataa
     2281 cgtcgttgtt gttcgtcgcc gcgcacagcc agtatcagaa cctgctgaca ctggcagaac
     2341 tgttcctggt ggggttgatt acatcagtgg ccaggatcag aagtggtggc ctgctgctgc
     2401 cggtattgct gcatatggaa gcaaccacgc tgggtttact gtttggttga aagttatatt
     2461 tttattaaac attgtgcgtt aaagcctggt gtgttttttt agtggatgtt atatttaaat
     2521 ataactttta tggaggtgaa gaatgcatac cacccgactg aagagggttg gcggctcagt
     2581 tatgctgacc gtcccaccgg cactgctgaa tgcgctgtct ctgggcacag ataatgaagt
     2641 tggcatggtc attgataatg gccggctgat tgttgagccg tacagacgcc cgcaatattc
     2701 actggctgag ctactggcac agtgtgatcc gaatgctgaa atatcagctg aagaacgaga
     2761 atggctggat gcaccggcga ctggtcagga ggaaatctga catggaaaga ggggaaatct
     2821 ggcttgtctc gcttgatcct accgcaggtc atgagcagca gggaacgcgg ccggtgctga
     2881 ttgtcacacc ggcggccttt aatcgcgtga cccgcctgcc tgttgttgtg cccgtaacca
     2941 gcggaggcaa ttttgcccgc actgccggct ttgcggtgtc gttggatggt gttggcatac
     3001 gtaccacagg tgttgtacgt tgcgatcaac cccggacaat tgatatgaaa gcacggggcg
     3061 gaaaacgact cgaacgggtt ccggagacta tcatgaacga agttcttggc cgcctgtcca
     3121 ctattctgac ttgaacatgg ggtttgaggg gcaactggat gaaaacgtac gatttagggc
     3181 actaaaaccg ctgttgtccc accattctgg tgattcccaa acgttatttg gctaaaaagt
     3241 agttttgatg tggtttattt tccgaattcc aagcgcagcc ctactttctt gtgcgtattt
     3301 gcgtgttttg cgcagttttg aagttccggt gatgctgcca acttactgat ttagtgtatg
     3361 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     3421 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
     3481 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
     3541 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaaccg cccgcattat
     3601 gggcgttggc ctcaacacga ttttccgcca tttaaaaaac tcaggccgca gtcggtaacc
     3661 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtgggga
     3721 tacgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag gctccggaag
     3781 acggttgttg cgcacgtatt ggggtctgac gctcagtgga acgaaaactc acgttaagca
     3841 acgttttctg cctctgacgc ctcttttaat ggtctcagat gtcctttggt caccagttct
     3901 gccagcgtga aggaataatg gccgagcata ttgatatgtc cgtggcaaag cggggagagg
     3961 cgtgcgatat cttcatcatt cagtgtttca ccctgcgccc ggagatgatc cagggctgcc
     4021 tgcatataaa tagtgttcca taacacgacg gcgttagtga ccagccccag tgcgcccagt
     4081 tgatcttcct gaccgtcggt atatcgtttt cttatctcac ctttttgacc gtgacagatg
     4141 gctctggcaa cggcatggcg actttctccc cgattaagct gggtcagaat gcgccggcgg
     4201 taatcttcat catcaatata attaagcaga tacagcgttt tgttgatgcg ccccacttca
     4261 atgattgcct gagtcagtcc ggaaggacgt tcacttttca gcaatgaacg gaccagcact
     4321 gaagcctgta ctttgcccag cttcagggag ccagcggtcc ggatcatttc gtcccactga
     4381 aggactattt ttggcactgt tgcaaagtta gcgatgaggc agccttttgt cttattcaaa
     4441 ggccttacat ttcaaaaact ctgcttacca ggcgcatttc gcccagggga tcaccataat
     4501 aaaatgctga ggcctggcct ttgcgtagtg cacgcatcac ctcaatacct ttgatggtgg
     4561 cgtaagccgt cttcatggat ttaaatccca gcgtggcgcc gattatccgt ttcagtttgc
     4621 catgatcgca ttcaatcacg ttgttccggt acttaatctg tcggtgttca acgtcagacg
     4681 ggcaccggcc ttcgcgtttg agcagagcaa gcgcgcgacc ataggcgggc gctttatccg
     4741 tgttgatgaa tcgcgggatc tgccacttct tcacgttgtt gaggatttta cccagaaacc
     4801 ggtatgcagc tttgctgtta cgacgggagg agagataaaa atcgacagtg cggccccggc
     4861 tgtcgacggc ccggtacaga tacgcccagc ggccattgac cttcacgtag gtttcatcca
     4921 tgtgccacgg gcaaagatcg gaagggttac gccagtacca gcgcagccgt ttttccattt
     4981 caggcgcata acgctgaacc cagcggtaaa tcgtggagtg atcgacattc actccgcgtt
     5041 cagccagcat ctcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg
     5101 cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca
     5161 tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg cctctcaaaa
     5221 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
     5281 gttggagtca ttaccaaaca gggattattt attctctcag gcgagaataa aatagacgaa
     5341 gaatgtttca ttaatatagt ccaaacggct gtcgccataa ataacatcgg tagagaatct
     5401 aatggttata ttttagttgg tgtttccgat accaaagcta cggcagacag agttaagact
     5461 ttatatggtg ttacccctat tgagtgcaat ggctattata tcaatggaat tgatcatgaa
     5521 gctgtaattc aatcaaaaaa catagataat tactttctgt tcataaaaca aaaaatagaa
     5581 tcatttaatt ttaatgaggc tttgaaacaa caaatactaa aagatatttc attatgttca
     5641 tatgaaggac tgcatgtttt aaaaatcgaa ataaaaagtg tcggagaagt ctgtcatttt
     5701 gaaaataggt attatataag acaaggaaca gctacgcatt taatcactga cggtgaagca
     5761 attagtagtt tacacagatt atattactca taattactta agcactatga aggtatcata
     5821 caccttcata gtgtactttt ttactgcgct ttggggctaa attgtttttg tgaatagcca
     5881 gaatatcgtg gctacggcgg caatggaaat atatgatctc tgctgccaga gactggagat
     5941 gtaattgggg gcagtttgga tatcgaaaat tattgtacta taaggggtcc gcttgagggg
     6001 gccgcttgga aaacggaaat tttcccacgc taagagaatt gagttgaacg agtggttaca
     6061 tctaggccag ctaactcact gaacgtaaag gctgtacgaa atgttccgac cgtccgataa
     6121 ccatttggct tacttccgta catttccttg cgtagatgta cagaattgta cgatttcgga
     6181 caccaaaact gctgttgtcc cggtgcatga agctactttc cgggttggtg gtactgactt
     6241 tggtgttctt aacgacgacg ttttcaactc tgaaaatcgc ctctctgcac ggggattttt
     6301 acccccccat agagccgtca tataaattca aattattaac tttgatggta gaattatctt
     6361 aggtttgata aaattttgag ccgtcgccgt ttacttcaca ggattcaata taatggctaa
     6421 atatacagtt tgtgattacc aataaactat tagaaataat gggaatggtt gtgccaatct
     6481 ctatctggag gtcttattgc agggaacatc caccccatcg ttacaccaat acagaattgc
     6541 ccccgataca agacatccag acattaacct aataaaagcg caccttgacg agggctttca
     6601 gcaggcaaag tctgagggat taaaagtaga gatttcagat tataaagaac gtctttacct
     6661 gtacattaga acacctggta ataacctaat gcaatattct ggttgcagag aaaaataata
     6721 cagtcgcgct ggatacgctg acattgacgc agttatatga ttttctggcg ttggtcagta
     6781 aaccagctgc agccaaaacg gtgtagtccc tgacacaggc gccggtcatt ctgttaactc
     6841 atccacgtat gggatgaaca gttgtttcag tttgaaacca gggatgtcag acggattttt
     6901 gctggcgagt acgaaatccg ttacgaactt aatggccaga ctatttatgt attgcgcctg
     6961 tgacacacac gagaaaacag gtagattgtg tgtgagggca actttaacat agcctgcagg
     7021 tgttaattca ggagcattgt tatcagacca aatatttttc attccattaa atagtcttgt
     7081 aggggataaa atgaattacg atgaaataac gaaaatcact gcagaacgta ttagcgacta
     7141 catgactgaa gcggttaata cagactctaa atctgttgcg gaaatgtttc ataacgctgc
     7201 atggggagtg ctatcattgt ggtttgaact cgtcacaaaa atagatctgg atatacataa
     7261 aaagaacagg tatgcaagtt atgattttag aagaaaaatc gaaatgcagc atgaagagtt
     7321 tcagaaaatg acagagcgtg agcaagtgcc tttattgaaa ttgcctgaat aacagagaca
     7381 gtgcaaacga ttatttcata ataacgatta tgtaatgact ggtaagaaaa taacttgatt
     7441 gagttccctt tatccagcct gatagtggat aaagggaact caataataat tgaattaatt
     7501 tattagcttc atcgcatttt ttttggtctc ttcatcactt ttttgctctt gttgtgaaga
     7561 ggattgagtg actattgaag aattaaaatc ggccatctct tttggaaata gcgatctcaa
     7621 tatattcacg atatctgaaa atgtggtttc tttcgtcagc agctcacata aaaggaaagg
     7681 ggtccgggga tcttgtctgt ataaggccag acctgccgtc agtgcggccc ggttaaggcg
     7741 gcttcgttcc ccttgcggga tactgtccag tgtatcacaa acaattttat ctgtttgatt
     7801 tacatctgga tttagtttga aggcaatggt tctgcgcttg tccatcatta attacctatg
     7861 agatacatac cgttaactaa atcatattga gagttattgg ttttgaaaaa acgttcatca
     7921 cgaatctgtg tgtgtttttt tactgcatcg catattaatt ctgcgccacc gcctataacc
     7981 ataacatgag tataaccaga aaattcattg agcgtattta atacacgttg ctcaagttta
     8041 cgaagtgctt cattcattgc ttcggtgact attgatattt tgttctcatc attaattcgt
     8101 tgcttcagat agttattatc ttttctgtga atgattatat cgtcagcaag atagctactt
     8161 ccttttgttc tcgcaagaga aagggcatct tttactgcag atgtaaccag agagacacca
     8221 agagatgagt ctccgtatat tttactgatc cccgataatt tccccattac ctgagaaata
     8281 tctaatgtgg tgcccccgag atctataatt aataaagaat ctaactcatc cagttcttgt
     8341 agaacttcat aacctgccgg tatagattca ggcatgactt ttacatcttt tattgtgaat
     8401 gtatccccgc catttaatgt aatttttttc cggaagtttg ctttcttacg ctcaatattt
     8461 tccgtattgg gttggttatt tctgtcgtaa tactctgtca gaggaagtgt gcaaacaata
     8521 tccacttcgc ttaccggcag accactggtc agtaaggcgt gatgcactgc aacgacatta
     8581 acgtcgctgt attgccatgc gatattggtt gtgactacag catccgggct gattggatca
     8641 aatgaatact gttcgccgtt cagtgtgtag ttaaagacct ttttatcacc aaaagagact
     8701 gcccactcgc gtttgaagct gttcgggcta atgtgctgtt taattgttcc gtcgctttcc
     8761 tgccactgta gtttgatgtt tgttgaaccg tcatcaatga ataccaacat ttataaaact
     8821 ccttatggtg tttttttggt gtgtgtttgg gtatgttttg ggttttaagt gggtttgttt
     8881 gtcaagttta ccccatttca accatcaatc aatgattatt tgtcttgttt tggtgtttta
     8941 ttgggttggg tatgggttgt ttttgggttt tgtttggcgg gtaacaaaag atgtcgccgg
     9001 gctcctcttt ttactgcgcc ggctgacgcg gcgcggcagg aacgctgcct gtggtcagtg
     9061 tcccgcgtcc ggccgggcac gggacgggcg cttttaccgc tcccggctgg cgttggtgac
     9121 agtgtacgcc agcccgtcgc ccttttctga tgaacgccct tcaagccgct ttcgcggcat
     9181 atcctcgccg tcaaaaagac gacggctgcg gtattccacg gtcggcctga cccgttaccg
     9241 gacgcggtga acagcccaca ggcagcgggg aacgggcacc acaggggtgc cctcccggtg
     9301 ccctctgtaa gagaaggagt gctgttatgt cccgttttat cctgggtgac tgcgtgcgtg
     9361 ttatggccac gtttccgggc aatgctgtcg atttcatcct caccgacccg ccgtatctcg
     9421 tcggtttccg tgaccgttcc gggcgcacca tcgccggcga taaaaccgat gaatggctgc
     9481 aaccagcctg taatgaaatg taccgcgtac tcaaaaaaga cgcgttaatg gtgagcttct
     9541 atggctggaa ccgcgtcgat cgctttatgg ccgcctggaa aaatgcggga ttcagcgttg
     9601 ttggtcacct ggtcttcacc aaaaactaca catcgaaggc cgcatatgtg ggctatcgcc
     9661 acgaatgtgc ctacatcctg gcaaaaggcc gtccacgtct gccacaaaac ccgctgccgg
     9721 acgtgctggg ctggaaatat tcgggcaatc gccatcaccc gacggaaaag cccgttacca
     9781 gcctgcaacc gctgattgag agcttcacac acccgaacgc aattgtgctg gacccgtttg
     9841 caggcagcgg ctcaacctgc gtcgccgccc tccagtccgg acgccggtat atcggtatcg
     9901 agctgcttga gcagtatcac cgtgccgggc agcaacgcct tgccgccgtg caacgggcca
     9961 tgcagcaggg ggccgcgaat gatgactggt ttatgccgga ggctgcgtaa atgaactatg
    10021 caggacacga aaaactgcgc gccgaagtgg cggaggtggc caatgccatg tgcgacctgc
    10081 gtacaaccat gaatgagatg gagcagcggt acagctttaa tgccgacacc ctgccggaac
    10141 gtctggtgcg tcagacgctg tttcgcgcaa accgcctcct gatggaggca tataccgaaa
    10201 ttctggaact ggaagcgtgc tttaaagatt gagaaggaga cgagaatgta cggaacatgc
    10261 gaaacgctat gccgggagct ggcagtaaag tatccgggag acatgccgct gatgctggtt
    10321 atctggtccc cggaagagat tcaggcactg gctgacggta tggaaatttc tctgaccggt
    10381 catgaaatca ggaccgtcct ggcacgcctg gaggacatcc cggaagacca gcggattgag
    10441 tccggtattt cttccgctgc ggtgatggag attatccgca acgagagcga aaaccgcctg
    10501 gtgaccgtcc ctgctgaact gctggcgtcc ctgattcaga ccgctgaaca ggcattgtgg
    10561 aaacgtgaat gggccgcccg ggatcatggc ctcgccgtcc cggaatgcgt cacccgccgt
    10621 caggaggtgg ttaatcaggc ccgcaccctg ctgaaaaaca acacacacga aaacgactga
    10681 tgttatcgcc gcctgccctg cgggcggcga tacagggaga acgcttatct gaatctgccc
    10741 acgctggatg aggccgtgaa taccctgaaa ccctggtggc cgggattgtt tgacggtgac
    10801 acgccccggc ttctggcctg cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg
    10861 aatatcccgc tctcgcataa aaaactgcgc agggcgctga aggccatcac ccgttcagaa
    10921 agctatctgt gtgccatgaa agccggtgcc tgccggtatg acacggaagg gtatgtgacg
    10981 gagcatattt ctcaggagga ggaagcgtat gcggcagcgc gtctggataa aatccgccgc
    11041 cagaaccgga taaaggcaga acttcaggcc gtgcttgatg agaaataaaa aaaagcctcc
    11101 atcagagagg aggcagggaa ataaggctta aaaggaaata acttccgtac ccgaaaggaa
    11161 gccaattcag tagattattt aacaccataa tactgacggg ttaaagagga gtgataaaga
    11221 tgaatggatt cagaaacagt tcacggaacg gtcaggtctg gcgctggcag cgtgccggaa
    11281 gccgtgccgt cagcctggag gtcaggggat tccggaagga ggccgctgaa gcgtggctcc
    11341 tggctgcccg tgttgctccc cgtacagact ggcagcagtt tgcacggcag agagccgaac
    11401 gctgtcagcg acggagataa tgaaaaaaaa cggcaaaaaa aagacttccc ctgagcaaag
    11461 gaggccaaaa atcattcata gcgtattact gacattgaat gaatgctctg tacatcaacc
    11521 ctgtgaagct accaccggtt aatgccggga ggttgcggga agaaagataa tacacagtca
    11581 ttacgtggtt aatatctgtc agaatgtttt ttgtgaccat aaactgtcag atggataaaa
    11641 aagaccgtca tgtgacggtc agtgttgtcc tgtcagggac aaatgcattc agtaaggaga
    11701 gcaaaataat aacatcacgt tgcccggctg ttaatatcag gcgatgttaa gtgttattta
    11761 tgactctttt atctggttgt gaagtctgtt aatcaggaag agaaaatggc gaccggaaaa
    11821 tcagagaagc aacaggtgat tgcctttatc gattacgaga actgcagtaa cctgacagaa
    11881 atttccttaa atatatatac ggagttaatt atattcgcgg gtgcgaaaca gaataatgtg
    11941 gtactgccag taaacgcttt ccctgaagca gtaaaaatca cactccggca tgtcagtgaa
    12001 gtctcccgga acaatgttga ttttcatctg gtgctggagc tggggcagtg tgtatgccac
    12061 tacgggccgg agaaggaata ccatattgtt tccgctgaca agggatatga cggtattgtc
    12121 cggacactgc aggcgcgcgg gattcgctgc cgtcgcgtct ccccggataa ggcgtcatca
    12181 gtaaagatgg ctgcacccga catggatatc gttatccggt gggcaaacaa aattcaacag
    12241 gcagcgcggg aggttcgtaa tatgccggta acagaaaaga catttaataa ttatttaaaa
    12301 tcacagatgc gcggtgaatg gcgtgatgtc ctgaccagag gtatccggga tgaactggtc
    12361 cgtcgcgggg taatgaaaat tcaggggaat aaaatcatct ggtaagactg ccagaataaa
    12421 atgatattta ttttatttat aaaaacagaa aggtcgaaaa tcaataatgg catttatttc
    12481 catgacaaga cagaaagcgc cgtacagata tgcaagcaag acccggttat cgataagacg
    12541 aacagactca ggctctgccg ggtgttatgt gacggttgcc gttcggggga agcaggaatt
    12601 tattattgct gaacttgatt cagaaatccg gaaaatcagg ctgaaactga cggactccta
    12661 tgaagaaggg gtaaggctgt cttcgggcac gtttacgctt ccggccagat tctgccggga
    12721 aatactgccg gacgacgtca ggagtatcac cattattctg gaaaaaagcg acgatgagtg
    12781 gtggtacggg agttactgat atgtggcaga actccggaca ctgacgcctt cgcgttgctc
    12841 agttgtccaa ccccggaaac gggaaaaagc aagttttccc cgctcccggc gtttctgcaa
    12901 taaaaagcag gggcatggag gtcgtcgtaa ccagacagga atgtggtatt atcgcggcgg
    12961 gtgtctgagc ctttctggtt caggcaagac gcaggtacca gaaatgcgaa gaccccactc
    13021 gttaatccat taactcgtga ggtctgcatg aagtacttag cactactgat tgtagcctct
    13081 tctttgcaga gaggtcaaag tttatgacga aatatgccct tatcgggttg ctcgccgtgt
    13141 gtgccacggt attgtgtttt tcactgatat tcagggaacg gttatgtgaa ctgaatattc
    13201 acaggggaaa tacagtggtg caggtaactc tggcctacga agcacggaag taagctgccg
    13261 ggcggggacg gaagtccccg ctttccggaa gtgtgaggta tttcaggggc agacacccga
    13321 catgccagaa acagccggtc ccgcccgggg ccggcatcca ggttaaggca tttcctgttt
    13381 ttcagtcatt tcatcatcaa aatcacatta aacggtcgta atcagacatg atttgtgcgc
    13441 caacacagat catcatcaca actttcaagt cgctgatttc aaaaaactgt agtatcctct
    13501 gcgaaacgat ccctgtttga gaattgagga ggcgagatgt cgcagacaga aaatgcagtg
    13561 acttcctcat caggtacaaa gcgagcatac cgaaaaggta atcctctgac gcttgcagaa
    13621 cgtcagcagg catctctggc aagaaaacgt gccacacata aagaactcag agtgttcatt
    13681 cctgctgcgt taaaagcgca attacaggag atgtgtgacg ctgaaggtgt tactcaggct
    13741 gagatgattg ctgaactgat aaagcagaag agtgctttca gctaactaaa atgtagcgac
    13801 atcacattct tgcctgtttg tggtgtgcgt gatagattgc tgatcgttta aggaattttg
    13861 tggctggcca cgccataagg tggcagggaa ctggttctga tgtggatgta caggagccag
    13921 aaaagcgaaa accccgataa tcttctttaa ctttggcgag tcagaaagat taccggggct
    13981 aacaagaaac tgcatagaag ctgttgctct atgcggggag tatagttata tgcccggaaa
    14041 agttcaagac ttctttctgt gctcactcct tctgtgcaac ataagtgcag gatggt
//
