LOCUS       NZ_CP103323             2445 bp    DNA     circular CON 15-SEP-2023
DEFINITION  Klebsiella pneumoniae strain hvKP12 plasmid phvKP12-H, complete
            sequence.
ACCESSION   NZ_CP103323
VERSION     NZ_CP103323.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30403289
            Assembly: GCF_024762135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Huang,J.
  TITLE     Emergence of a fatal ST11-KL64 Tigecycline-resistant hypervirulent
            Klebsiella pneumoniae clone co-carrying blaNDM and blaKPC in
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Department of Laboratory Medicine, The First
            Affiliated Hospital of Chongqing Medical University, 1 Youyi Road,
            Yuzhong District, Chongqing 400016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/15/2023 04:03:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,116
            CDSs (total)                      :: 5,995
            Genes (coding)                    :: 5,813
            CDSs (with protein)               :: 5,813
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 64 of 182
            Pseudo Genes (incomplete)         :: 124 of 182
            Pseudo Genes (internal stop)      :: 22 of 182
            Pseudo Genes (multiple problems)  :: 28 of 182
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103323.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hvKP12"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="phvKP12-H"
                     /geo_loc_name="China: Chongqing"
                     /collection_date="2021-03-10"
     gene            326..1294
                     /gene="mobV"
                     /locus_tag="NXZ72_RS30450"
                     /old_locus_tag="NXZ72_30450"
     CDS             326..1294
                     /gene="mobV"
                     /locus_tag="NXZ72_RS30450"
                     /old_locus_tag="NXZ72_30450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1364..1594)
                     /locus_tag="NXZ72_RS30455"
                     /old_locus_tag="NXZ72_30455"
     CDS             complement(1364..1594)
                     /locus_tag="NXZ72_RS30455"
                     /old_locus_tag="NXZ72_30455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(1581..2063)
                     /locus_tag="NXZ72_RS30460"
                     /old_locus_tag="NXZ72_30460"
     CDS             complement(1581..2063)
                     /locus_tag="NXZ72_RS30460"
                     /old_locus_tag="NXZ72_30460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          318..1291
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tcagccaaag ctgcggcgag cggttcctgc tctttcaaag acgatttcat tgcctgattt
       61 cgtcaagggc gagcgtagcg agtgccattt acccttgacg acactccgga taaaacaggc
      121 ttggcagtgg tggggggtgg cgcgaagcga cgccttccac ctgccagacc gccgttgatt
      181 ttgaccttct ggcagcttgc tgcccgaacg attttgcgta gcaaagtcta gtgagtttta
      241 cactatcatc ttgatagtgg cccgccggag gtatcgcaat gcgctccccc gcctggctga
      301 atgaatgccg gaatcagggg ggaacatggc gaactacgcg ataatgcgct gcaaaaagct
      361 gacgggaatg ggaagcgtgg ccagcgcgtt gcagcactgc taccgagagc gtgagacgcc
      421 gaacgctaac gctgaacgga caccagaaaa ctactgctct gtgtccaaat caacggatca
      481 ggcaatgggg cgtgtgcggg agctgctgcc ggaaaaacgc cgtaaagatg cggtactggc
      541 cgttgaatac gttatgacag caagccctga gtggtggaaa gaggccacac cacggcagca
      601 ggcggagttt ttcgcccgct ctgagcaatg gctggagaag aaatacggga aggatcgtgt
      661 tgtggcggcg gtggttcacc gggacgaggc caccccgcac ctgtctgcgt tcgtggtgcc
      721 attgacgcag gatggacggc tgtccgcgaa agagtttatt ggcgggcggt caaagatgcg
      781 tgaagatcag agcacctacg ctgaatcagt aaaaaaactg ggtcttgagc gcggcatcga
      841 gggaagccgc gccacgcatc agacagttca gcactactac gaatcgataa accgtggcac
      901 ccgtagccag gtatcgatct cgccggaggc gctggagccg cgtgtattgc ggaagggcat
      961 tttcaccaaa gacgtggaag atcaggcagc catagccaaa cgtctaagcc acgcagtgaa
     1021 cgatggcttt gcaggaacca tcgctatggc ctctcagagc gcccagaacg caaaaagagc
     1081 gcgtgagcta cagaagacta tggatgcgca gcagaaacgc ctacagagcg tcacagagcc
     1141 ttttaagggg ctttccagag agcagatgac cgaaatcctg atgatggctc agcggtttaa
     1201 gcagcaaaac caggaaaagg aaaaacagca gcgtattgaa cgtgaaaaac agcgacagac
     1261 gcgatcgcgt ggcatgggtg gtatggagcg ttagttttga tgcgatgcag gatagggcta
     1321 acgccgcaaa gtgacgtcat ttgcggcatt caccacgaaa aaattaatca ttcagatggt
     1381 aattatgttt ttttaaaata gaaaaagctg cgtttttctg ctttggtgac aaacccttag
     1441 ttgagtattt attaccgcta gttctacgat atatgctgta aacactgctt aaaaaagtac
     1501 gctcccaaac agtcagaacc tcattctttt ttagtgaatc atgcagcatt agcctgtcta
     1561 catttttaaa aagtttatct tcagcattca tcatccatta ccttcgtctg caccgttctt
     1621 ccattggtgt cataactgat ctttaactca attttctttc ttctttcgta aattgatggc
     1681 cagtctcccc gtgcaaaaag aaaggggtta gcttcaagtt ccccacgccc tgcccggaag
     1741 aatatttgtt tatctacaag agccgcaatt atgtttttaa aggtctgata ttcaatatga
     1801 accctgggct gtatggtttt ttcaaatcta gtttttgata cagacacaaa cccatcccat
     1861 ccaatcagca ttgccaattg taaaagcaca tcattttgag attttgataa accatgaatc
     1921 atcgcaatgt ctgcaatgta tagcttgacg tagggaggtt cggctgggat ggaaaaggtc
     1981 tcgatcttag agtcttgttt tacctcaccg ttttcatcca tgtatgtatg ccgttcaagc
     2041 ctagaaatct ttttcgtatt cactccagcc ccacgtttcg ccatgattta gtactcgcat
     2101 ttaagtacta aagagacata cagtcaagtc ctttgtatca agtacgatta gtacttgagg
     2161 tgagtacgat tagtccttga ggtgagtacg attagtactt gaggtgagta cgattggtac
     2221 ttgaggtgag tacgattctt cgctctagcc cttggtacaa gcgggttccc atatctatat
     2281 ctatatatta agggggaaaa taagggctga aaaacggcat ttttagacac tgaaaaactg
     2341 ccttttttta gcaaggagtg tacgcatttc tatgtacggg gagtggaaat tccgcttcct
     2401 cgctttggct gttcgttcct cgtcgcgttg ctcctgcggt acgcc
//
