LOCUS       NZ_CP103322             5596 bp    DNA     circular CON 15-SEP-2023
DEFINITION  Klebsiella pneumoniae strain hvKP12 plasmid phvKP12-G, complete
            sequence.
ACCESSION   NZ_CP103322
VERSION     NZ_CP103322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30403289
            Assembly: GCF_024762135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Huang,J.
  TITLE     Emergence of a fatal ST11-KL64 Tigecycline-resistant hypervirulent
            Klebsiella pneumoniae clone co-carrying blaNDM and blaKPC in
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Department of Laboratory Medicine, The First
            Affiliated Hospital of Chongqing Medical University, 1 Youyi Road,
            Yuzhong District, Chongqing 400016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/15/2023 04:03:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,116
            CDSs (total)                      :: 5,995
            Genes (coding)                    :: 5,813
            CDSs (with protein)               :: 5,813
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 64 of 182
            Pseudo Genes (incomplete)         :: 124 of 182
            Pseudo Genes (internal stop)      :: 22 of 182
            Pseudo Genes (multiple problems)  :: 28 of 182
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hvKP12"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="phvKP12-G"
                     /geo_loc_name="China: Chongqing"
                     /collection_date="2021-03-10"
     gene            120..365
                     /locus_tag="NXZ72_RS30400"
                     /old_locus_tag="NXZ72_30400"
     CDS             120..365
                     /locus_tag="NXZ72_RS30400"
                     /old_locus_tag="NXZ72_30400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            complement(547..891)
                     /locus_tag="NXZ72_RS30405"
                     /old_locus_tag="NXZ72_30405"
     CDS             complement(547..891)
                     /locus_tag="NXZ72_RS30405"
                     /old_locus_tag="NXZ72_30405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(881..1057)
                     /locus_tag="NXZ72_RS30410"
                     /old_locus_tag="NXZ72_30410"
     CDS             complement(881..1057)
                     /locus_tag="NXZ72_RS30410"
                     /old_locus_tag="NXZ72_30410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1697..1855
                     /locus_tag="NXZ72_RS30615"
     CDS             1697..1855
                     /locus_tag="NXZ72_RS30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2213..2539
                     /locus_tag="NXZ72_RS30415"
                     /old_locus_tag="NXZ72_30415"
     CDS             2213..2539
                     /locus_tag="NXZ72_RS30415"
                     /old_locus_tag="NXZ72_30415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            2565..3110
                     /locus_tag="NXZ72_RS30420"
                     /old_locus_tag="NXZ72_30420"
     CDS             2565..3110
                     /locus_tag="NXZ72_RS30420"
                     /old_locus_tag="NXZ72_30420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3176..3526
                     /locus_tag="NXZ72_RS30425"
                     /old_locus_tag="NXZ72_30425"
     CDS             3176..3526
                     /locus_tag="NXZ72_RS30425"
                     /old_locus_tag="NXZ72_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3596..3862)
                     /locus_tag="NXZ72_RS30430"
                     /old_locus_tag="NXZ72_30430"
     CDS             complement(3596..3862)
                     /locus_tag="NXZ72_RS30430"
                     /old_locus_tag="NXZ72_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3846..4070)
                     /locus_tag="NXZ72_RS30435"
                     /old_locus_tag="NXZ72_30435"
     CDS             complement(3846..4070)
                     /locus_tag="NXZ72_RS30435"
                     /old_locus_tag="NXZ72_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(4737..4949)
                     /locus_tag="NXZ72_RS30440"
                     /old_locus_tag="NXZ72_30440"
     CDS             complement(4737..4949)
                     /locus_tag="NXZ72_RS30440"
                     /old_locus_tag="NXZ72_30440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4956..5465)
                     /locus_tag="NXZ72_RS30445"
                     /old_locus_tag="NXZ72_30445"
     CDS             complement(4956..5465)
                     /locus_tag="NXZ72_RS30445"
                     /old_locus_tag="NXZ72_30445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1144..1220)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1409..1806
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(4..557)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.31e-116"
     Typing          complement(4622..5595)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.692"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcccctgacg aggcgagggc cagtaagccc cgcgtaatag cctctgcggc ttcctgcttc
       61 gttttgggta acgaagatgg cgtcaccagc gcgcgccggt tctccggtgc gttcgggtcg
      121 tgcagcccca gtcgcccgtt caccacggtc tgccacgcat cgatgcgcgg acggtctgcc
      181 cggtcgtagt acggctggag acgcctgccg gtcagcagtt ccgtgttcgg taccagaaaa
      241 ttcagttcca gccgcccctt gtcccggtgt tcaacccaca gcacgctgta ctggtcttta
      301 tcgagtccgg gcatcagaac ccgctcgaag ctcgccatca gcttttcacg ctggccgggc
      361 ggtaaatcct gttcagcaaa agacaggacg ccggaggtgt actttttggc gtagggcgag
      421 gcgtcgataa gctcccggac ctcctccggc tttccctgca ggacgatggc cccgtcgcgc
      481 tggcggtctt tccccagcag ataatccacc ggcccggcac cgccgcctcg ccctcgcgga
      541 tgaaacttaa cgatcatcgt ctgccccctt ttccagcacg gcatgccgca gccgctcgag
      601 ttcggcatca atggccatca gcgcggccac cacctgaacc cggtcagcgc ctgaccactg
      661 gccgccgtta attttccggg cgatctggtt gaggttgttc cccatcccgg caagctggcg
      721 cagcaaaacc ggatcgatgg acggcagccg caacgaccgc gccgggcgcg tatcgagaca
      781 ggtttcccgc atccacaccg ccagttgcct tccattgcag cgctccagca gctgctggta
      841 ctccccatcg gtcacccgga tacctaaaaa acgggtacgc ttaaccgcca tcctctcctc
      901 cgatatccgc aaaatgtgac cgggctgaac gctcaagctc tgccatctgc tcccggcggt
      961 aatcttcctc ctgtgcctga cgcatcagat ttgccatcac tgccggatgc caggatcttt
     1021 tttttacgac gagcggcttc ttcttcggct tctgcattat ccctccttcg caaacgccgg
     1081 acggcacgga aaagttttcc gggtcgctcc ggcgttggac aagcgccagc gcgtcagtgg
     1141 tgttagcggg tttcggggcg cagccctgaa ccagtcacag cgtttacgct gtgtatactg
     1201 gcttactatg ttggcatgag agcacgattc atgaaagtgc ctcatgtggc aggtgtgata
     1261 tgtggctcag gagcgtccgc aataccatgc acggacagat gaaatgacgc ctcctcgctc
     1321 actcggtagc tgcgctcctg ccgttcggct gcggcgggcg tgaccgactc acggataacg
     1381 ggataaagca aaggcgctca aatcagaaag ggccggagaa aaggatgcgg cgttgccgtt
     1441 tttccatagg ctccgcctcc cctgacgagc atcacagaaa tggacgctca agtcagagga
     1501 ggacgaaacc cgacaggact taaagatgcc ggtgatttct ctccggcggc tccctcgtgc
     1561 gctctcctgt tccgaccctg ccgtttaccg gatacctgtt ccgccttttt ccctttcggg
     1621 aagcgtggcg ctttctcata gctcacgctg ctggtatctc ggctcggttt aggtcgttcg
     1681 ctccaagctg ggctgtatgc acgaaccccc cgttcagccc gactgctgcg ccctttccgg
     1741 taactatcat cttgagtcca acccggacgg acacgcaaaa acgccactgg cagcagccat
     1801 tggtaactgg atatatacga gagagatatt gagaggtctt gaagtgggtg cctaactgcg
     1861 gctacactga aagaacagtt ttggtatctg cggtctcgta tacccagtta cccggttaag
     1921 cagttcccca actgacttaa ccttcgatca aaccgcctcc ccaggcggtt ttttcgttta
     1981 cagtgcaaga gattacgcgc agaaaaaaag gatctcaaga agatccttta aatgatcgtc
     2041 agttctgtta tccccggagc tccatccatc ccacggaaaa acacccgcca ctgatgccct
     2101 cagcgcactg cacatcatgt acaaaccact ccctccctcc ttttttattg cgtttcttaa
     2161 tgcgcaatgg tagctttgtg cgcaacaaaa aacgcaatag caaggggggg taatgagaga
     2221 aagaacaaat atcatgctag atataggatt aaagcaatcc ttacaacggc tttcgaataa
     2281 aaccaaaatg agtctaagtg atcttattaa ccagagatta tcaggttcac tttcagttga
     2341 agaacgcatt ggcgaaaagc ctgaatggat gagaaacctt gatccctatc tcgccaatca
     2401 cttttcaaat attgagttac cgctcccttc accattaaaa gaataccaag aaaaaatgga
     2461 tgagatagat tcaattatta atgacatgat ggttctaaag gaatatttca gagctaagtt
     2521 ttacgacgaa ataaaatagg cacgctatac ggaggcggag aattatgttt tttaaacggt
     2581 atttaacgga gaaggaaata tacaaggcag cactcccaat tgttaggctt caagggagag
     2641 ttaacacttc attacgtttt ttatccaaat actcagatgt aaacaaaaat ggattaattg
     2701 cattgcatct taatttatta cattttatct catacaacat acacggtgta gagtctctga
     2761 aaatcactca agcaggcata aacaaagctg tacaacaaag ccctcacgca gatgaaataa
     2821 aaaatattgc gcaggcaata ggacgcaaag gacctgagta catcgaagga ttaattagaa
     2881 acccatgggg gaagaaaaat gataatccac taatatttat aatgagcatt gaagaaagta
     2941 taaaagaaac ccttaactca ggttatatgt tatatagagt agctcccata actgagggtg
     3001 aagcagctgc tttcatggat gttcttaaac aattattatt atttgaggat aattttcgcc
     3061 tttttggtag agacttggtt tataaaatag aaaaattaaa aatcagataa caaagtatgg
     3121 tgaacgaaaa atgaaagaga ggatagtgaa atgtgtaata ccaagattct caactatgta
     3181 ttggttaatt gcgttcctag catttatatt ccccggtcga tcttttgctg acggttacac
     3241 cgcttactac cgatgtaatg gtactcagga agcatctctt actgttaaag ataacaaact
     3301 aagtatggtt gtaggtcttt ctaagtttaa taggcaactg gatacttata gattcaaaga
     3361 aaagggaatc gaagtagtaa tgcagagagc tgcttctgat gataactctg aggccataat
     3421 gtcttttatt atgcctgacg ttaacaatat gaccttatta gttaaaaata acggctacat
     3481 tatcgttaag acaacctgct ggttaactaa aaccgacaaa cagtagaaca cgtagttagt
     3541 cgagtattta ccataatacc tgttgtcggc atcaaaaaag cccgcatagc gggcttcagt
     3601 cgtaaacttc gcggcgatgt ccaatcgtgg cggcaaggat aacaagctcg tcatctcgga
     3661 tctcacacag aacgcgataa tctcccaccc gatagcgcca gaactcgccc agctcacctt
     3721 tgagcggctt ccctgactgg cgaggatcgg cagcaactgc aatgcgtaaa ctcataaaat
     3781 ccacaatccg tcgtgcgttc tgtttgtcca tcttgcgtaa cgatttgagc gcccgatcgg
     3841 aatagttaat cgtccagacc aagtgacttc ctcacgtctt cagacgaatg cactgcttca
     3901 tcaccacggc gaacgcgtgc agcagtttct gctgaaagat aataatcctc caggtcttcc
     3961 agatgcgcca gtattgcttc ccgcgcataa aacgtctttg ttctgccggt ttgcttcgcc
     4021 agcgagtcca gacgggactc aatctcatca gataaccgga tagcaagcat ataaacctcc
     4081 cgaacaccat ttgctataca tgtatatcaa cagtaccaca atcaacctaa aagaacaaaa
     4141 aataatcttg ttagcccctc ttgataatcc gtaacgcggt caatatatct agcttgaggg
     4201 ctttcctctt aactattgaa aggataacga ttatgggtct taagccgggt ccgaaacgaa
     4261 ttgccgattc aacaggggaa cctgataaac gccagcgtga caataaaaaa acgccgggaa
     4321 atactgacaa attgaaaccc agcaaatctt caaaaaaata agtcagtagt taaaagccct
     4381 ccggggcttt tttgtatctg ccgttcacgc tgattaaaca taacacccgt tgcccgcacc
     4441 gccccggacg ggttcgccgc gaacggggtg gcagtcaggt aaaaccggca acaaaccacc
     4501 cgataaaccg ccccgttcgc gacagataaa ttttacctgg ctgcatagcc atgcagccag
     4561 gtgaatgaca tccggtttca gcgtaaatgc ccgatgagaa ccctgttttc ctgcatttca
     4621 gcccagcgac ggaccgtcat agcttcgctg ccgggaacgc gcttccgtca gttccttttc
     4681 gtgctgccgt tgctcgtgcg cccgttctgc ggccacggcc atctcatgac gctgcactac
     4741 cggctcaaac gctgcagttg cccgctcaag cgttccacct gctgactcaa gcgcgtcacg
     4801 cgctcgctca gcgccgcgtt ctcccgttgc ataagccctg acattttccg ccattccccg
     4861 aaggcgctct cccactcgtc cagccttttc gagtagtcct gctgtagctg ctctaatgcg
     4921 ctcagcaact gtttttccag ctcggtcatc ggcacttact ccttcaccag tctcagcgcg
     4981 ttctgcttcg accgcccgtt cccgaccgtc cagctctgcg tcctgtctac ggccgtcccc
     5041 ttcggcagca ccagaaaacg tccgttgctg tcttccaggt acgtcacgcc ccaggtcttc
     5101 gcgttcagct tcgacagcgc tgcgttctgc cggtcgattt cggccagatt tgacgctatc
     5161 aagctcccct gataccacaa caccccgctc aggctggcga acagcagtcc tgacaccatc
     5221 gtgatggtca gccatgtccg cccgaccata cgcatcacgc tcaggcggtt cgactgcatg
     5281 gccgcgctca taccctgctc gtgggcgcgg atggcggcgc tgattctctt cccgctttcg
     5341 ctcagttccg ctctgacaga cttctcgtgc tcgctgaatg cggctttcag catttcgccg
     5401 gtactctgct gctgcgcttt cgatttctgc tctaagtcct tcgccagcgt taaaagactg
     5461 ttcatagatg gctcccttaa gtcggatgtt tcgccctccg tccgggtcgg caatgctgat
     5521 gctgctcttt gtggtgcgca cgacctcaaa acctgcgctt tccagcgcct cagtgacgtc
     5581 ctgacgcgtt ttaagc
//
