LOCUS       NZ_CP103252             2743 bp    DNA     circular CON 14-SEP-2023
DEFINITION  Bacteroides uniformis strain BFG-227 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP103252
VERSION     NZ_CP103252.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30323235
            Assembly: GCF_024760085.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides uniformis
  ORGANISM  Bacteroides uniformis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 354x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/14/2023 06:06:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,944
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,652
            CDSs (with protein)               :: 3,652
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 149 of 213
            Pseudo Genes (incomplete)         :: 57 of 213
            Pseudo Genes (internal stop)      :: 19 of 213
            Pseudo Genes (multiple problems)  :: 11 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103252.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Bacteroides uniformis"
                     /mol_type="genomic DNA"
                     /strain="BFG-227"
                     /host="Homo sapiens"
                     /db_xref="taxon:820"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1986-07-30"
     gene            64..654
                     /locus_tag="NXX72_RS19715"
                     /old_locus_tag="NXX72_19715"
     CDS             64..654
                     /locus_tag="NXX72_RS19715"
                     /old_locus_tag="NXX72_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_004295513.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     gene            1272..1907
                     /locus_tag="NXX72_RS19720"
                     /old_locus_tag="NXX72_19720"
     CDS             1272..1907
                     /locus_tag="NXX72_RS19720"
                     /old_locus_tag="NXX72_19720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_258896393.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSIQCIRQTQGRTKPASSASERTTFH"
                     /name="plasmid recombination protein"
     gene            1963..2367
                     /locus_tag="NXX72_RS19725"
                     /old_locus_tag="NXX72_19725"
     CDS             1963..2367
                     /locus_tag="NXX72_RS19725"
                     /old_locus_tag="NXX72_19725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151871045.1"
                     /translation="MIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQNGTK
                     AFKAENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALR
                     HHFPNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="hypothetical protein"
     Typing          32..644
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1273..1832
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.465"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          1835..2346
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtttttat ggaaaataat agtttacttt gtggagaata atatttccca aaaacacatc
       61 aaaatggaaa ataaaaaagc agttaagtta accgattttc aaaagaacga agaaaatcct
      121 tttatgaaac aagctataga aggtattgaa aatcatgttg ttaaaaagta taagagtaat
      181 agtggtggcg ataagagagc cgtagtagct ttagccgaca ctgaaactgg agaagtgttt
      241 aagacttcgt ttatccgtca aatagaagta gatgaagaac aattcactaa attgtatctt
      301 tctaactttg ctgcattctt tgacctatca caagcagcta ttcgggtttt tggttacttt
      361 atgacctgca tgaaacccaa aaatgattta atcatcttca atagaaaaaa atgcctagaa
      421 tataccaaat acaaaacaga caaagccgtt tataaaggac ttgcagaact tgtaaaagct
      481 gaaatcatag cccgaggacc agccgataat ctttggttta ttaatcctct gatagtattc
      541 aatggtgacc gagtgacatt tgctaaaaca tacgttcgga aaaagacttt agctgcccaa
      601 aagaaagaag aagcagagaa acgacaatta tcacttggct ttgatgaaca gtaacactcc
      661 attgagtgaa gctgccgttt ggtcgctccc cctttggggg cgggggggga tagataaagt
      721 tcctctatgt aaagttataa tgggggatga aaggcaaggt tcgctaacct taccccgatt
      781 gtttatgctt cccagcattc aatctatccc cgtttatggg tttctctttc accctacaaa
      841 gataacctca tgggggaaaa atgtccaaga atatggggta aaaactatca agtcggtaga
      901 aaataagtat ctttgaagta catttttagt agaggtactg gtatgcctag acgaagcaga
      961 taggcaaaaa taaagccgct ggagatgtcg gatgtccgtc aattaactcc agcggcttgt
     1021 attttggtaa ctctcccgta tcggtcggct accgtttggt cagcaacaaa ggtagtactt
     1081 tattgtcgca aatccaaatg ttttcccctt tatttgtgca tttacagaac atttgctaac
     1141 ttcgcaccta tgcagtatgg tgatagcaca ctagcaccct aaagggtgcg tgtgtaaggg
     1201 gacaagcccc cttaaccccc tgtcagccgg ctgtcaccgg ctgttatcga ttgtcacaaa
     1261 aatgttaact tatgggagca acaagtattc atgtacaagc agtgaagccg gggagtgaga
     1321 ttcacaactt tagggaaaaa gagttggact atgttcgtcc cgaacttagt catttgaatg
     1381 aaagctgggt tggagatagc atctcccatc ggctggaaag tgcgaaacaa agatatctcg
     1441 atacggttgg gcagaagatg caggctaaag ccgcacccat acgagaggga gtaatagtaa
     1501 tcaaacaaga aaccaccatg caggaactcc agcagtttgc cacggtctgc aaagaacgtt
     1561 tcggtatcga agcatttcaa atccatatac acaaggacga aggatacatg aacgcaaagc
     1621 aatggacacc taaccttcat gcccatgtag ttttcgattg gacgcagccg aatgggaaaa
     1681 gtgtgcgttt atcgcgtgat gacatggcag aactccaaac catagcatct gaaacactgg
     1741 gcatggaacg tggcgtttct tctgaccgca aacacttatc ggctatgcag tacaagaccg
     1801 aatgtgcgaa agaacagctt caggaactat caatccagtg cattagacaa acacaaggac
     1861 gtacaaaacc agcttcttca gcttcagaaa gaactacgtt ccattgaaac aaagaagaac
     1921 gtccaaaaac tcatttctaa ggcttcagag aagttttacg gcttgattgg aaaaacagtc
     1981 aacgataggg aaaaggatgc cttaaaagcc aaagtaaagg ctttagaggg ggaaaatgaa
     2041 cagctatccg atagactggg aaaggctata cttgaaaaag agcaaaacgg cacaaaggca
     2101 ttcaaagccg agaatgacaa ggagtattat agacaacaaa tggacaacgc aaggacaacc
     2161 agtaaccgat taaggacgga gaaccaagaa ctaaagaccg aaaccaagga attgaaaaaa
     2221 gaacttggta agatgaaaga cttgttcaat tccgaacaac tggaggcttt aaggcatcat
     2281 ttcccaaaca tatcaaaagc tatggaagag gggaaagacc tactcaagca aatcaccaga
     2341 agcagaggtt tcggtatggg tatgtaaccc cccccccccc ccaaaggggg gagcgaccaa
     2401 acggcagcct ctctcaatgg agtgttacgt cgttcggatt cggaagaaat tttgcagggt
     2461 tcggaaaacg gttgtgtgct tattctaaaa aaagtttatg gtgtggcgct cgtcgggacg
     2521 ggtggtcccg ccccttgccg ctgggcgctc cccgaccgat gatttttaag tgactgattt
     2581 tgtgctgttt tgggggtata ttaagaatag aagaaataga ataagttaag tacttgatac
     2641 acaatatagg gcattttcca tattggaaat tctcattttc caatctagaa aatactgatt
     2701 tcctaatata atactaaaca ggaaaataat atttccctta ata
//
