LOCUS       NZ_CP103251             4185 bp    DNA     circular CON 14-SEP-2023
DEFINITION  Bacteroides uniformis strain BFG-227 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP103251
VERSION     NZ_CP103251.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30323235
            Assembly: GCF_024760085.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides uniformis
  ORGANISM  Bacteroides uniformis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 354x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/14/2023 06:06:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,944
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,652
            CDSs (with protein)               :: 3,652
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 149 of 213
            Pseudo Genes (incomplete)         :: 57 of 213
            Pseudo Genes (internal stop)      :: 19 of 213
            Pseudo Genes (multiple problems)  :: 11 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103251.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Bacteroides uniformis"
                     /mol_type="genomic DNA"
                     /strain="BFG-227"
                     /host="Homo sapiens"
                     /db_xref="taxon:820"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1986-07-30"
     gene            1..198
                     /locus_tag="NXX72_RS19680"
                     /old_locus_tag="NXX72_19680"
     CDS             1..198
                     /locus_tag="NXX72_RS19680"
                     /old_locus_tag="NXX72_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295503.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            391..689
                     /gene="mbpA"
                     /locus_tag="NXX72_RS19780"
                     /pseudo=""
     CDS             391..689
                     /gene="mbpA"
                     /locus_tag="NXX72_RS19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /name="mbpA"
     gene            686..1480
                     /gene="mbpB"
                     /locus_tag="NXX72_RS19685"
                     /old_locus_tag="NXX72_19685"
     CDS             686..1480
                     /gene="mbpB"
                     /locus_tag="NXX72_RS19685"
                     /old_locus_tag="NXX72_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_007560590.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="mbpB"
     gene            1437..1889
                     /gene="mbpC"
                     /locus_tag="NXX72_RS19690"
                     /old_locus_tag="NXX72_19690"
     CDS             1437..1889
                     /gene="mbpC"
                     /locus_tag="NXX72_RS19690"
                     /old_locus_tag="NXX72_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_004295511.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="mbpC"
     gene            1909..2139
                     /locus_tag="NXX72_RS19695"
                     /old_locus_tag="NXX72_19695"
     CDS             1909..2139
                     /locus_tag="NXX72_RS19695"
                     /old_locus_tag="NXX72_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295510.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="hypothetical protein"
     gene            complement(2176..3240)
                     /locus_tag="NXX72_RS19700"
                     /old_locus_tag="NXX72_19700"
     CDS             complement(2176..3240)
                     /locus_tag="NXX72_RS19700"
                     /old_locus_tag="NXX72_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_258896392.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEINRNKETFITAQEKITDLIGELALLNGKSREKNNPK
                     GWIINALKGKIKDK"
                     /name="replication initiation protein"
     gene            3652..3903
                     /locus_tag="NXX72_RS19705"
                     /old_locus_tag="NXX72_19705"
     CDS             3652..3903
                     /locus_tag="NXX72_RS19705"
                     /old_locus_tag="NXX72_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295507.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            3900..4175
                     /locus_tag="NXX72_RS19710"
                     /old_locus_tag="NXX72_19710"
     CDS             3900..4175
                     /locus_tag="NXX72_RS19710"
                     /old_locus_tag="NXX72_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="WP_004327436.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
ORIGIN
        1 atggtcgaat attgtgttta ctggttagag aacggcgagc ctatgcacga ggtgttctct
       61 tctcttgccg ccgccgagat gtactcatgt gcgataagag ggaaagaaaa cattgaatgg
      121 gtggaggtgt ccgaagaaga aaccattgat ttggacgaac tggaagacat gttcccggat
      181 gacttctgcg gcgtgtaatc cccttgggta gaaatggggt agaatttgtt aggctgtggg
      241 ggattttcta acacgccggg gctttttgct gggcggttgg cgtggatgta ttcccatgcg
      301 gcatgtgtat atatagcaag aagtgtcctt gtcggacaat tcttgctttt ctcgctttgc
      361 tcaaaaagat tttaagatta cctttgtggc atggaactaa gacggaacga aaagattaca
      421 ttccggtgta ccgaacttga aaaggacgca cttgcggagc aggcagcccg gtgcagtcta
      481 agcgtatcgg aatactgccg gagtttgtcc cttgggggcg cccaagggag aggtacaccg
      541 aggaggaacg gcagcttctc cgggacatag cccagttgaa aggaacgctc caacggctga
      601 acaactactt cggtggtcgc cagtaccggg aagtgttcga ggagaaccgg gcactaatta
      661 cagagctcaa aaaaatactc tcacgatgat agggaaaggg aaaagcatat cacatggtgt
      721 ggcggcattg gagtacgacc tcgccaaaga gataaacggg caggcagtag ccaccgagat
      781 agcccggcat gaactttatg ggtgtactgg tgcggaaatg gtgcaggaaa tgaaaccgta
      841 tcacattgat tttccgaacg taaaaaacaa ctgcctacgt ttcgaggtca gcccctcgat
      901 agaggaaagc gccaccttta cggatgcgga ctgggcggaa cttggtaacg acttcatgca
      961 gcgcatgggg ttggcgaacc accagtacat catcatccgg cacagcggta cggagagcaa
     1021 gaaagagcaa gcccacctgc acatactggc aaaccgggtt tccctttccg gggaactgta
     1081 ccgggacaac tggataggga aaaaggcaac ggaagccgcc aatgccatag ccaaggaacg
     1141 gaactttgta cagtcgcagg acattggcaa agtcaacaaa gcggaaatca aggaagccat
     1201 ggacggcgta ttgaagaaga tgcagggctt tgactttacc aaattcaagg aagaacttgg
     1261 taagagaggt tttaaagtcc gggaagccag ggcaagcacc gggaaactta acggctacta
     1321 tgtcacagcc cgaagcggta cggagtataa ggcaagcgag atagggaaag gctacactct
     1381 cgcccatatc gagcggacac aaagcaaact gaagtgcaac tcaatgaaca tatctcatgg
     1441 aaacaaactc acacccggaa gcggcagctt ccaacgttaa gcagggacag tacagccccc
     1501 ggaaaagaaa agatacaacg ccaagggtaa acccgcagct tgcggtagaa ctggtgtatc
     1561 aagaactaaa gcgtgttgaa gtctatacga agcgcataga ggacgcaaca gcccgaaagg
     1621 tacaaataga cgggaaaagc cttgaaagtg ccgaaaatcg cctaaaaaac gtgttggcgg
     1681 actttgaacg gcaggggtac agaatgaaaa acggcggtta tgtggacaaa cgaatttcgt
     1741 tctactcaat cctttgtgct gttatctccc tattgttcgc ttgtttcatg tgctatcttt
     1801 ggacggatgc agccaaagac cgggacaact acaagcagta ttatgaatac taccaagagc
     1861 aggcaaggga acaaaagggg aataaataaa catctaactt tacgaattat gggaacaacg
     1921 gaacacgaag aaccccggtt cttcttcata ctgaacaagg gtgcgaagtc cggcggcgaa
     1981 atcacccatg ccgtcctaaa cggtagtatc gtttcgaagc cggcaggctg ggacgccttt
     2041 catgggcttg cactggcgag agagaaacta agcagcgagg aaatacaaca gcagatgaaa
     2101 gaacttggtg tagaaatgga aatcgttcct ctgatttgac aagattaccc ggagtgtttc
     2161 agccgggtaa tcttgttact tatccttgat tttcccttta agggcgttta taatccaccc
     2221 tttcggattg ttcttttctc gtgattttcc gtttaggaga gccagttctc cgataaggtc
     2281 ggttatcttt tcttgtgccg ttatgaatgt ctctttgttc cggtttatct cttgtgccgt
     2341 tatgaatgtc tctttgttcc ggtttatctc ttccgatgtg aagccgcagg aataacggag
     2401 gtactcgtac acatggctgt ctatctgata tcgtgctgta acctttgctt gcaattcttt
     2461 cccttccagt tcttcatctc tgaactgtgg ttgatagacc gggtagaacc taaacccggt
     2521 caccttgcta cgtttgttgt ttgggttttc acgcactttc acgtagttga aagtgtatgg
     2581 gcagctctca tcaagcgcag cctttgctgg tttcaaaact ctttcctcaa aatgatctat
     2641 tctgtctttt ccgttcttgt ccttgtattt gtccgcaggt atgccaagac ggtctttcaa
     2701 gttttcaagg gatatatcca aagggtacac ctgcccactc attaacatgt aaaacctaag
     2761 ggaataccca gtaggtagcg caagagcctt gtttaactca aattctctgt accccttggc
     2821 gaacttggta aacacatccc aaaggtcgtt agacacacga aaggttataa tgccagtgcg
     2881 ttttttatat tttgggttag atataaatcc gcacttccac cattcctcgt catcttcgta
     2941 agtaaaaaaa cgtcctgcca aagaatcgag tgcggctatg atttcattgt aatcacgtcc
     3001 ggaaaaaata acgtcagata catgcatttg tgcatcaaca tcccaaagtc catgctcaaa
     3061 cttgcgtttc gtgcctgcat aatctttcag ttttactccc tttagttcag cttggcacgc
     3121 ttccagtatg cgaagaacta ttctttgttc gtgtatggac atgtcttgct gttttgacca
     3181 tgtatatacc catgaaacaa caacatcttt attcttggta ataggtagtt tctttttcat
     3241 aaacgattga tttgctgcaa atatacacaa tccccacatt aaaacaaaaa agaggggtaa
     3301 aactcccaaa gtgtggggac taatttactt ttaaactccc aaagtgtggg gactaaactc
     3361 ccaaagtgtg gggaaacaca tgttctaatc cctttattta tgggcgtttg cgaagaagtg
     3421 atagagcata ttacagacga tgcttataac agacaagggg aatttataga gctaagtggt
     3481 tgatttactg tcggaaaaag aaaaacgata gggaaaaaga gcaaagcaaa accaaccaac
     3541 gggcggtagg attggaaacg ggtttttatg gcgtgtacac gtttgtgtac aaaatgtttg
     3601 tattttattg aaatatagct atctttgtgc tgaatttaaa agcaaacaga tatggaagca
     3661 ttatcagtaa gagaataccg taacaacctc gcagcgtctt tcaccaaagc tgacaatggc
     3721 gagcaggtac taattcgcag gaaaaacgag atttacgctt tggtaaaagt tggtcgtgaa
     3781 gatttgatga taaccccgga gctgcaagca aggattgaca aggcaaggga agaaatcaaa
     3841 tccggaaagt gtgttaccct caaaagcagt gaggatattg acgcttattt cgatagtcta
     3901 tgatgtacac aattagggtt tccgacgggg tggataaggt gattgccaaa tggaagaaat
     3961 caaaccccaa tttgttcaag aagtataaga aaatctacaa agaattgtta gagcacccta
     4021 agactgggct tgggcatccc gaagcgttaa ggggcggtgg ggatattaca tggtctcgac
     4081 acattaccgc ccatgaccgg ataatctatg acatttacga ggaagtggta gaggtctaca
     4141 tcttggaggt agaggggcat tacaacgata aatgataaaa ctgtt
//
