LOCUS       NZ_CP103240             2713 bp    DNA     circular CON 14-SEP-2023
DEFINITION  Bacteroides thetaiotaomicron strain BFG-130 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP103240
VERSION     NZ_CP103240.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30323177
            Assembly: GCF_024759965.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides thetaiotaomicron
  ORGANISM  Bacteroides thetaiotaomicron
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 21x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/14/2023 06:54:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,403
            CDSs (total)                      :: 5,301
            Genes (coding)                    :: 4,984
            CDSs (with protein)               :: 4,984
            Genes (RNA)                       :: 102
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 269 of 317
            Pseudo Genes (incomplete)         :: 51 of 317
            Pseudo Genes (internal stop)      :: 16 of 317
            Pseudo Genes (multiple problems)  :: 17 of 317
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103240.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Bacteroides thetaiotaomicron"
                     /mol_type="genomic DNA"
                     /strain="BFG-130"
                     /host="Homo sapiens"
                     /db_xref="taxon:818"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1985-10-21"
     gene            1..1104
                     /locus_tag="NXV61_RS27000"
                     /old_locus_tag="NXV61_27000"
     CDS             1..1104
                     /locus_tag="NXV61_RS27000"
                     /old_locus_tag="NXV61_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_007219893.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQNGTKAFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="plasmid recombination protein"
     gene            1543..2133
                     /locus_tag="NXV61_RS27005"
                     /old_locus_tag="NXV61_27005"
     CDS             1543..2133
                     /locus_tag="NXV61_RS27005"
                     /old_locus_tag="NXV61_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_004295513.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     Typing          1511..2123
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1101
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.275"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggagcaa caagtattca tgtacaagca gtgaagccgg ggagtgagat tcacaacttt
       61 agggaaaaag agttggacta tgttcgtccc gaacttagtc atttgaatga aagctgggtt
      121 ggagatagca tctcccatcg gctggaaagt gcgaaacaaa gatatctcga tacggttggg
      181 cagaagatgc aggctaaagc cgcacccata cgagagggag taatagtaat caaacaagaa
      241 accaccatgc aggaactcca gcagtttgcc acggtctgca aagaacgttt cggtatcgaa
      301 gcatttcaaa tccatataca caaggacgaa ggatacatga acgcaaagca atggacacct
      361 aaccttcatg cccatgtagt tttcgattgg acgcagccga atgggaaaag tgtgcgttta
      421 tcgcgtgatg acatggcaga actccaaacc atagcatctg aaacactggg catggaacgt
      481 ggcgtttctt ctgaccgcaa acacttatcg gctatgcagt acaagaccga atgtgcgaaa
      541 gaacagcttc aggaactatc aaacgatata tccagtgcat tagacaaaca caaggacgta
      601 caaaaccagc ttcttcagct tcagaaagaa ctacgttcca ttgaaacaaa gaagaacgtc
      661 caaaaactca tttctaaggc ttcagagaag ttttacggct tgattggaaa aacagtcaac
      721 gatagggaaa aggatgcctt aaaagccaaa gtaaaggctt tagaggggga aaatgaacag
      781 ctatccgata gactgggaaa ggctatactt gaaaaagagc aaaacggcac aaaggcattc
      841 aaagccgaga atgacaagga gtattataga caacaaatgg acaacgcaag gacaaccagt
      901 aaccgattaa ggacggagaa ccaagaacta aagaccgaaa ccaaggaatt gaaaaaagaa
      961 cttggtaaga tgaaagactt gttcaattcc gaacaactgg aggctttaag gcatcatttc
     1021 ccaaacatat caaaagctat ggaagagggg aaagacctac tcaagcaaat caccagaagc
     1081 agaggtttcg gtatgggtat gtaacccccc cccgccccca aagggggagc gaccaaacgg
     1141 cagcctctct caatggagtg ttacgtcgtt cggattcgga agaaattttg cagggttcgg
     1201 aaaacggttg tgtgcttatt ctaaaaaaag tttatggtgt ggcgctcgtc gggacgggtg
     1261 gtcccgcccc ttgccgctgg gcgctccccg accgatgatt tttaagtgac tgattttgtg
     1321 ctgttttggg ggtatattaa gaatagaaga aatagaataa gttaagtact tgatacacaa
     1381 tatagggcat tttccatatt ggaaattctc attttccaat ctagaaaata ctgatttcct
     1441 aatataatac taaacaggaa aataatattt cccttaatat tgtttttatg gaaaataata
     1501 gtttactttg tggagaataa tatttcccaa aaacacatca aaatggaaaa taaaaaagca
     1561 gttaagttaa ccgattttca aaagaacgaa gaaaatcctt ttatgaaaca agctatagaa
     1621 ggtattgaaa atcatgttgt taaaaagtat aagagtaata gtggtggcga taagagagcc
     1681 gtagtagctt tagccgacac tgaaactgga gaagtgttta agacttcgtt tatccgtcaa
     1741 atagaagtag atgaagaaca attcactaaa ttgtatcttt ctaactttgc tgcattcttt
     1801 gacctatcac aagcagctat tcgggttttt ggttacttta tgacctgcat gaaacccaaa
     1861 aatgatttaa tcatcttcaa tagaaaaaaa tgcctagaat ataccaaata caaaacagac
     1921 aaagccgttt ataaaggact tgcagaactt gtaaaagctg aaatcatagc ccgaggacca
     1981 gccgataatc tttggtttat taatcctctg atagtattca atggtgaccg agtgacattt
     2041 gctaaaacat acgttcggaa aaagacttta gctgcccaaa agaaagaaga agcagagaaa
     2101 cgacaattat cacttggctt tgatgaacag taacactcca ttgagtgaag ctgccgtttg
     2161 gtcgctcccc ctttgggggc ggggggggga tagataaagt tcctctatgt aaagttataa
     2221 tggggtatgc ttcccagcat tcaatctatc cccgtttatg ggtttctctt tcaccctaca
     2281 aagataacct catgggggaa aaatgtccaa gaatatgggg taaaaactat caagtcggta
     2341 gaaaataagt atctttgaag tacattttta gtagaggtac tggtatgcct agacgaagca
     2401 gataggcaaa aataaagccg ctggagatgt cggatgtccg tcaattaact ccagcggctt
     2461 gtattttggt aactctcccg tatcggtcgg ctaccgtttg gtcagcaaca aaggtagtac
     2521 tttattgtcg caaatccaaa tgttttcccc tttatttgtg catttacaga acatttgcta
     2581 acttcgcacc tatgcagtat ggtgatagca cactagcacc ctaaagggtg cgtgtgtaag
     2641 gggacaagcc cccttaaccc cctgtcagcc ggctgtcacc ggctgttatc gattgtcaca
     2701 aaaatgttaa ctt
//
