LOCUS       NZ_CP103180             2737 bp    DNA     circular CON 14-SEP-2023
DEFINITION  Parabacteroides distasonis strain BFG-158 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP103180
VERSION     NZ_CP103180.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30323199
            Assembly: GCF_024759645.1
KEYWORDS    RefSeq.
SOURCE      Parabacteroides distasonis
  ORGANISM  Parabacteroides distasonis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae;
            Parabacteroides.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 236x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/14/2023 06:06:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,238
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 57 of 103
            Pseudo Genes (internal stop)      :: 7 of 103
            Pseudo Genes (multiple problems)  :: 15 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103180.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Parabacteroides distasonis"
                     /mol_type="genomic DNA"
                     /strain="BFG-158"
                     /host="Homo sapiens"
                     /db_xref="taxon:823"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1985-10-28"
     gene            64..654
                     /locus_tag="NXY22_RS21725"
                     /old_locus_tag="NXY22_21725"
     CDS             64..654
                     /locus_tag="NXY22_RS21725"
                     /old_locus_tag="NXY22_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_004295513.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     gene            1272..2363
                     /locus_tag="NXY22_RS21730"
                     /old_locus_tag="NXY22_21730"
     CDS             1272..2363
                     /locus_tag="NXY22_RS21730"
                     /old_locus_tag="NXY22_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_258918844.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEHIHK
                     DEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVSSDRK
                     HLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQKLISK
                     ASEKFYGLIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQNGTKAFKAEND
                     KEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHFPNIS
                     KAMEEGKDLLKQITRSRGFGMGM"
                     /name="mobilization protein"
     Typing          32..644
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1273..2360
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtttttat ggaaaataat agtttacttt gtggagaata atatttccca aaaacacatc
       61 aaaatggaaa ataaaaaagc agttaagtta accgattttc aaaagaacga agaaaatcct
      121 tttatgaaac aagctataga aggtattgaa aatcatgttg ttaaaaagta taagagtaat
      181 agtggtggcg ataagagagc cgtagtagct ttagccgaca ctgaaactgg agaagtgttt
      241 aagacttcgt ttatccgtca aatagaagta gatgaagaac aattcactaa attgtatctt
      301 tctaactttg ctgcattctt tgacctatca caagcagcta ttcgggtttt tggttacttt
      361 atgacctgca tgaaacccaa aaatgattta atcatcttca atagaaaaaa atgcctagaa
      421 tataccaaat acaaaacaga caaagccgtt tataaaggac ttgcagaact tgtaaaagct
      481 gaaatcatag cccgaggacc agccgataat ctttggttta ttaatcctct gatagtattc
      541 aatggtgacc gagtgacatt tgctaaaaca tacgttcgga aaaagacttt agctgcccaa
      601 aagaaagaag aagcagagaa acgacaatta tcacttggct ttgatgaaca gtaacactcc
      661 attgagtgaa gctgccgttt ggtcgctccc cctttggggg cgggggggga tagataaagt
      721 tcctctatgt aaagttataa tgggggatga aaggcaaggt tcgctaacct taccccgatt
      781 gtttatgctt cccagcattc aatctatccc cgtttatggg tttctctttc accctacaaa
      841 gataacctca tgggggaaaa atgtccaaga atatggggta aaaactatca agtcggtaga
      901 aaataagtat ctttgaagta catttttagt agaggtactg gtatgcctag acgaagcaga
      961 taggcaaaaa taaagccgct ggagatgtcg gatgtccgtc aattaactcc agcggcttgt
     1021 attttggtaa ctctcccgta tcggtcggct accgtttggt cagcaacaaa ggtagtactt
     1081 tattgtcgca aatccaaatg ttttcccctt tatttgtgca tttacagaac atttgctaac
     1141 ttcgcaccta tgcagtatgg tgatagcaca ctagcaccct aaagggtgcg tgtgtaaggg
     1201 gacaagcccc cttaaccccc tgtcagccgg ctgtcaccgg ctgttatcga ttgtcacaaa
     1261 aatgttaact tatgggagca acaagtattc atgtacaagc agtgaagccg gggagtgaga
     1321 ttcacaactt tagggaaaaa gagttggact atgttcgtcc cgaacttagt catttgaatg
     1381 aaagctgggt tggagatagc atctcccatc ggctggaaag tgcgaaacaa agatatctcg
     1441 atacggttgg gcagaagatg caggctaaag ccgcacccat acgagaggga gtaatagtaa
     1501 tcaaacaaga aaccaccatg caggaactcc agcagtttgc cacggtctgc aaagaacgtt
     1561 tcggtatcga acatatacac aaggacgaag gatacatgaa cgcaaagcaa tggacaccta
     1621 accttcatgc ccatgtagtt ttcgattgga cgcagccgaa tgggaaaagt gtgcgtttat
     1681 cgcgtgatga catggcagaa ctccaaacca tagcatctga aacactgggc atggaacgtg
     1741 gcgtttcttc tgaccgcaaa cacttatcgg ctatgcagta caagaccgaa tgtgcgaaag
     1801 aacagcttca ggaactatca aacgatatat ccagtgcatt agacaaacac aaggacgtac
     1861 aaaaccagct tcttcagctt cagaaagaac tacgttccat tgaaacaaag aagaacgtcc
     1921 aaaaactcat ttctaaggct tcagagaagt tttacggctt gattggaaaa acagtcaacg
     1981 atagggaaaa ggatgcctta aaagccaaag taaaggcttt agagggggaa aatgaacagc
     2041 tatccgatag actgggaaag gctatacttg aaaaagagca aaacggcaca aaggcattca
     2101 aagccgagaa tgacaaggag tattatagac aacaaatgga caacgcaagg acaaccagta
     2161 accgattaag gacggagaac caagaactaa agaccgaaac caaggaattg aaaaaagaac
     2221 ttggtaagat gaaagacttg ttcaattccg aacaactgga ggctttaagg catcatttcc
     2281 caaacatatc aaaagctatg gaagagggga aagacctact caagcaaatc accagaagca
     2341 gaggtttcgg tatgggtatg taaccccccc cgcccccaaa gggggagcga ccaaacggca
     2401 gcctctctca atggagtgtt acgtcgttcg gattcggaag aaattttgca gggttcggaa
     2461 aacggttgtg tgcttattct aaaaaaagtt tatggtgtgg cgctcgtcgg gacgggtggt
     2521 cccgcccctt gccgctgggc gctccccgac cgatgatttt taagtgactg attttgtgct
     2581 gttttggggg tatattaaga atagaagaaa tagaataagt taagtacttg atacacaata
     2641 tagggcattt tccatattgg aaattctcat tttccaatct agaaaatact gatttcctaa
     2701 tataatacta aacaggaaaa taatatttcc cttaata
//
