LOCUS       NZ_CP103129             4153 bp    DNA     circular CON 14-SEP-2023
DEFINITION  Parabacteroides distasonis strain BFG-162 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP103129
VERSION     NZ_CP103129.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30323201
            Assembly: GCF_024759425.1
KEYWORDS    RefSeq.
SOURCE      Parabacteroides distasonis
  ORGANISM  Parabacteroides distasonis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae;
            Parabacteroides.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 305x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/14/2023 06:02:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,478
            CDSs (total)                      :: 4,373
            Genes (coding)                    :: 4,142
            CDSs (with protein)               :: 4,142
            Genes (RNA)                       :: 105
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 188 of 231
            Pseudo Genes (incomplete)         :: 48 of 231
            Pseudo Genes (internal stop)      :: 18 of 231
            Pseudo Genes (multiple problems)  :: 17 of 231
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103129.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Parabacteroides distasonis"
                     /mol_type="genomic DNA"
                     /strain="BFG-162"
                     /host="Homo sapiens"
                     /db_xref="taxon:823"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1986-07-14"
     gene            join(3403..4153,1..44)
                     /gene="mbpB"
                     /locus_tag="NXW35_RS22395"
                     /old_locus_tag="NXW35_22395"
     CDS             join(3403..4153,1..44)
                     /gene="mbpB"
                     /locus_tag="NXW35_RS22395"
                     /old_locus_tag="NXW35_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_007560590.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="mbpB"
     gene            1..453
                     /gene="mbpC"
                     /locus_tag="NXW35_RS22400"
                     /old_locus_tag="NXW35_22400"
     CDS             1..453
                     /gene="mbpC"
                     /locus_tag="NXW35_RS22400"
                     /old_locus_tag="NXW35_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_004295511.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="mbpC"
     gene            473..703
                     /locus_tag="NXW35_RS22405"
                     /old_locus_tag="NXW35_22405"
     CDS             473..703
                     /locus_tag="NXW35_RS22405"
                     /old_locus_tag="NXW35_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295510.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="hypothetical protein"
     gene            complement(740..1765)
                     /locus_tag="NXW35_RS22410"
                     /old_locus_tag="NXW35_22410"
     CDS             complement(740..1765)
                     /locus_tag="NXW35_RS22410"
                     /old_locus_tag="NXW35_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004295509.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            2178..2434
                     /locus_tag="NXW35_RS22415"
                     /old_locus_tag="NXW35_22415"
                     /pseudo=""
     CDS             2178..2434
                     /locus_tag="NXW35_RS22415"
                     /old_locus_tag="NXW35_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2431..2706
                     /locus_tag="NXW35_RS22420"
                     /old_locus_tag="NXW35_22420"
     CDS             2431..2706
                     /locus_tag="NXW35_RS22420"
                     /old_locus_tag="NXW35_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="WP_004327436.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            2717..2914
                     /locus_tag="NXW35_RS22425"
                     /old_locus_tag="NXW35_22425"
     CDS             2717..2914
                     /locus_tag="NXW35_RS22425"
                     /old_locus_tag="NXW35_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295503.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            3107..3406
                     /gene="mbpA"
                     /locus_tag="NXW35_RS22430"
                     /old_locus_tag="NXW35_22430"
     CDS             3107..3406
                     /gene="mbpA"
                     /locus_tag="NXW35_RS22430"
                     /old_locus_tag="NXW35_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_004327437.1"
                     /translation="MELRRNEKITFRCTELEKDALAEQAARCSLSVSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="mbpA"
     Typing          3404..4146
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.452"
                     /coverage="94.0"
                     /evalue="5.12e-139"
ORIGIN
        1 atggaaacaa actcacaccc ggaagcggca gcttccaacg ttaagcaggg acagtacagc
       61 ccccggaaaa gaaaagatac aacgccaagg gtaaacccgc agcttgcggt agaactggtg
      121 tatcaagaac taaagcgtgt tgaagtctat acgaagcgca tagaggacgc aacagcccga
      181 aaggtacaaa tagacgggaa aagccttgaa agtgccgaaa atcgcctaaa aaacgtgttg
      241 gcggactttg aacggcaggg gtacagaatg aaaaacggcg gttatgtgga caaacgaatt
      301 tcgttctact caatcctttg tgctgttatc tccctattgt tcgcttgttt catgtgctat
      361 ctttggacgg atgcagccaa agaccgggac aactacaagc agtattatga atactaccaa
      421 gagcaggcaa gggaacaaaa ggggaataaa taaacatcta actttacgaa ttatgggaac
      481 aacggaacac gaagaacccc ggttcttctt catactgaac aagggtgcga agtccggcgg
      541 cgaaatcacc catgccgtcc taaacggtag tatcgtttcg aagccggcag gctgggacgc
      601 ctttcatggg cttgcactgg cgagagagaa actaagcagc gaggaaatac aacagcagat
      661 gaaagaactt ggtgtagaaa tggaaatcgt tcctctgatt tgacaagatt acccggagtg
      721 tttcagccgg gtaatcttgt tacttatcct tgattttccc tttaagggcg tttataatcc
      781 accctttcgg attgttcttt tctcgtgatt ttccgtttag gagagccagt tctccgataa
      841 ggtcggttat cttttcttgt gccgttatga atgtctcttt gttccggttt atctcttccg
      901 atgtgaagcc gcaggaataa cggaggtact cgtacacatg gctgtctatc tgatatcgtg
      961 ctgtaacctt tgcttgcaat tctttccctt ccagttcttc atctctgaac tgtggttgat
     1021 agaccgggta gaacctaaac ccggtcacct tgctacgttt gttgtttggg ttttcacgca
     1081 ctttcacgta gttgaaagtg tatgggcagc tctcatcaag cgcagccttt gctggtttca
     1141 aaactctttc ctcaaaatga tctattctgt cttttccgtt cttgtccttg tatttgtccg
     1201 caggtatgcc aagacggtct ttcaagtttt caagggatat atccaaaggg tacacctgcc
     1261 cactcattaa catgtaaaac ctaagggaat acccagtagg tagcgcaaga gccttgttta
     1321 actcaaattc tctgtacccc ttggcgaact tggtaaacac atcccaaagg tcgttagaca
     1381 cacgaaaggt tataatgcca gtgcgttttt tatattttgg gttagatata aatccgcact
     1441 tccaccattc ctcgtcatct tcgtaagtaa aaaaacgtcc tgccaaagaa tcgagtgcgg
     1501 ctatgatttc attgtaatca cgtccggaaa aaataacgtc agatacatgc atttgtgcat
     1561 caacatccca aagtccatgc tcaaacttgc gtttcgtgcc tgcataatct ttcagtttta
     1621 ctccctttag ttcagcttgg cacgcttcca gtatgcgaag aactattctt tgttcgtgta
     1681 tggacatgtc ttgctgtttt gaccatgtat atacccatga aacaacaaca tctttattct
     1741 tggtaatagg tagtttcttt ttcataaacg attgatttgc tgcaaatata cacaatcccc
     1801 acattaaaac aaaaaaagag gggtaaaact cccaaagtgt ggggactaat ttacttttaa
     1861 actcccaaag tgtggggact aaactcccaa agtgtgggga aacacatgtt ctaatccctt
     1921 tatttatggg cgtttgcgaa gaagtgatag agcatattac agacgatgct tataacagac
     1981 aaggggaatt tatagagcta agtggttgat ttactgtcgg aaaaagaaaa acgataggga
     2041 aaaagagcaa agcaaaacca accaacgggc ggtaggattg gaaacgggtt tttatggcgt
     2101 gtacacgttt gtgtacaaaa tgtttgtatt ttattgaaat atagctatct ttgtgctgaa
     2161 tttaaaagca aacagatatg gaagcattat cagtaagaga ataccgtaac aacctcgcag
     2221 cgtctttcac caaagctgac aatggcgagc aggtactaat tcgcaggaaa aacgagattt
     2281 acgctttggt aaaagttggt cgtgaagatt tgatgataac cccggagctg caagcaagga
     2341 ttgacaaggc aagggaagaa atcaaatccg gaaagtgtgt taccctcaaa agcagtgagg
     2401 atattatatt gacgcttatt tcgatagtct atgatgtaca caattagggt ttccgacggg
     2461 gtggataagg tgattgccaa atggaagaaa tcaaacccca atttgttcaa gaagtataag
     2521 aaaatctaca aagaattgtt agagcaccct aagactgggc ttgggcatcc cgaagcgtta
     2581 aggggcggtg gggatattac atggtctcga cacattaccg cccatgaccg gataatctat
     2641 gacatttacg aggaagtggt agaggtctac atcttggagg tagaggggca ttacaacgat
     2701 aaatgataaa actgttatgg tcgaatattg tgtttactgg ttagagaacg gcgagcctat
     2761 gcacgaggtg ttctcttctc ttgccgccgc cgagatgtac tcatgtgcga taagagggaa
     2821 agaaaacatt gaatgggtgg aggtgtccga agaagaaacc attgatttgg acgaactgga
     2881 agacatgttc ccggatgact tctgcggcgt gtaatcccct tgggtagaaa tggggtagaa
     2941 tttgttaggc tgtgggggat tttctaacac gccggggctt tttgctgggc ggttggcgtg
     3001 gatgtattcc catgcggcat gtgtatatat agcaagaagt gtccttgtcg gacaattctt
     3061 gcttttctcg ctttgctcaa aaagatttta agattacctt tgtggcatgg aactaagacg
     3121 gaacgaaaag attacattcc ggtgtaccga acttgaaaag gacgcacttg cggagcaggc
     3181 agcccggtgc agtctaagcg tatcggaata ctgccggagt ttgtcccttg gggggcgccc
     3241 aagggagagg tacaccgagg aggaacggca gcttctccgg gacatagccc agttgaaagg
     3301 aacgctccaa cggctgaaca actacttcgg tggtcgccag taccgggaag tgttcgagga
     3361 gaaccgggca ctaattacag agctcaaaaa aatactctca cgatgatagg gaaagggaaa
     3421 agcatatcac atggtgtggc ggcattggag tacgacctcg ccaaagagat aaacgggcag
     3481 gcagtagcca ccgagatagc ccggcatgaa ctttatgggt gtactggtgc ggaaatggtg
     3541 caggaaatga aaccgtatca cattgatttt ccgaacgtaa aaaacaactg cctacgtttc
     3601 gaggtcagcc cctcgataga ggaaagcgcc acctttacgg atgcggactg ggcggaactt
     3661 ggtaacgact tcatgcagcg catggggttg gcgaaccacc agtacatcat catccggcac
     3721 agcggtacgg agagcaagaa agagcaagcc cacctgcaca tactggcaaa ccgggtttcc
     3781 ctttccgggg aactgtaccg ggacaactgg atagggaaaa aggcaacgga agccgccaat
     3841 gccatagcca aggaacggaa ctttgtacag tcgcaggaca ttggcaaagt caacaaagcg
     3901 gaaatcaagg aagccatgga cggcgtattg aagaagatgc agggctttga ctttaccaaa
     3961 ttcaaggaag aacttggtaa gagaggtttt aaagtccggg aagccagggc aagcaccggg
     4021 aaacttaacg gctactatgt cacagcccga agcggtacgg agtataaggc aagcgagata
     4081 gggaaaggct acactctcgc ccatatcgag cggacacaaa gcaaactgaa gtgcaactca
     4141 atgaacatat ctc
//
