LOCUS       NZ_CP103121             4160 bp    DNA     circular CON 14-SEP-2023
DEFINITION  Bacteroides thetaiotaomicron strain BFG-510 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP103121
VERSION     NZ_CP103121.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30323413
            Assembly: GCF_024759205.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides thetaiotaomicron
  ORGANISM  Bacteroides thetaiotaomicron
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/14/2023 06:41:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,412
            CDSs (total)                      :: 5,323
            Genes (coding)                    :: 5,001
            CDSs (with protein)               :: 5,001
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 322
            CDSs (without protein)            :: 322
            Pseudo Genes (ambiguous residues) :: 0 of 322
            Pseudo Genes (frameshifted)       :: 246 of 322
            Pseudo Genes (incomplete)         :: 74 of 322
            Pseudo Genes (internal stop)      :: 29 of 322
            Pseudo Genes (multiple problems)  :: 24 of 322
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103121.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Bacteroides thetaiotaomicron"
                     /mol_type="genomic DNA"
                     /strain="BFG-510"
                     /host="Homo sapiens"
                     /db_xref="taxon:818"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2011-03-31"
     gene            1..1026
                     /locus_tag="NXW82_RS27020"
                     /old_locus_tag="NXW82_27020"
     CDS             1..1026
                     /locus_tag="NXW82_RS27020"
                     /old_locus_tag="NXW82_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004295509.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1063..1293)
                     /locus_tag="NXW82_RS27025"
                     /old_locus_tag="NXW82_27025"
     CDS             complement(1063..1293)
                     /locus_tag="NXW82_RS27025"
                     /old_locus_tag="NXW82_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295510.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="hypothetical protein"
     gene            complement(1313..1765)
                     /gene="mbpC"
                     /locus_tag="NXW82_RS27030"
                     /old_locus_tag="NXW82_27030"
     CDS             complement(1313..1765)
                     /gene="mbpC"
                     /locus_tag="NXW82_RS27030"
                     /old_locus_tag="NXW82_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_004295511.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="mbpC"
     gene            complement(1722..2529)
                     /gene="mbpB"
                     /locus_tag="NXW82_RS27035"
                     /old_locus_tag="NXW82_27035"
                     /pseudo=""
     CDS             complement(1722..2529)
                     /gene="mbpB"
                     /locus_tag="NXW82_RS27035"
                     /old_locus_tag="NXW82_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /name="mbpB"
     gene            complement(2526..2825)
                     /gene="mbpA"
                     /locus_tag="NXW82_RS27175"
     CDS             complement(2526..2825)
                     /gene="mbpA"
                     /locus_tag="NXW82_RS27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_004327437.1"
                     /translation="MELRRNEKITFRCTELEKDALAEQAARCSLSVSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="mbpA"
     gene            complement(3018..3215)
                     /locus_tag="NXW82_RS27040"
                     /old_locus_tag="NXW82_27040"
     CDS             complement(3018..3215)
                     /locus_tag="NXW82_RS27040"
                     /old_locus_tag="NXW82_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295503.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(3226..3501)
                     /locus_tag="NXW82_RS27045"
                     /old_locus_tag="NXW82_27045"
     CDS             complement(3226..3501)
                     /locus_tag="NXW82_RS27045"
                     /old_locus_tag="NXW82_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="WP_004327436.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3498..3749)
                     /locus_tag="NXW82_RS27050"
                     /old_locus_tag="NXW82_27050"
     CDS             complement(3498..3749)
                     /locus_tag="NXW82_RS27050"
                     /old_locus_tag="NXW82_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295507.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     Typing          complement(2003..2529)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.386"
                     /coverage="99.0"
                     /evalue="3.68e-97"
ORIGIN
        1 atgaaaaaga aactacctat taccaagaat aaagatgttg ttgtttcatg ggtatataca
       61 tggtcaaaac agcaagacat gtccatacac gaacaaagaa tagttcttcg catactggaa
      121 gcgtgccaag ctgaactaaa gggagtaaaa ctgaaagatt atgcaggcac gaaacgcaag
      181 tttgagcatg gactttggga tgttgatgca caaatgcatg tatctgacgt tattttttcc
      241 ggacgtgatt acaatgaaat catagccgca ctcgattctt tggcaggacg tttttttact
      301 tacgaagatg acgaggaatg gtggaagtgc ggatttatat ctaacccaaa atataaaaaa
      361 cgcactggca ttataacctt tcgtgtgtct aacgaccttt gggatgtgtt taccaagttc
      421 gccaaggggt acagagaatt tgagttaaac aaggctcttg cgctacctac tgggtattcc
      481 cttaggtttt acatgttaat gagtgggcag gtgtaccctt tggatatatc ccttgaaaac
      541 ttgaaagacc gtcttggcat acctgcggac aaatacaagg acaagaacgg aaaagacaga
      601 atagatcatt ttgaggaaag agttttgaaa ccagcaaagg ctgcgcttga tgagagctgc
      661 ccatacactt tcaactacgt gaaagtgcgt gaaaacccaa acaacaaacg tagcaaggtg
      721 accgggttta ggttctaccc ggtctatcaa ccacagttca gagatgaaga actggaaggg
      781 aaagaattgc aagcaaaggt tacagcacga tatcagatag acagccatgt gtacgagtac
      841 ctccgttatt cctgcggctt cacatcggaa gagataaacc ggaacaaaga gacattcata
      901 acggcacaag aaaagataac cgaccttatc ggagaactgg ctctcctaaa cggaaaatca
      961 cgagaaaaga acaatccgaa agggtggatt ataaacgccc ttaaagggaa aatcaaggat
     1021 aagtaacaag attacccggc tgaaacactc cgggtaatct tgtcaaatca gaggaacgat
     1081 ttccatttct acaccaagtt ctttcatctg ctgttgtatt tcctcgctgc ttagtttctc
     1141 tctcgccagt gcaagcccat gaaaggcgtc ccagcctgcc ggcttcgaaa cgatactacc
     1201 gtttaggacg gcatgggtga tttcgccgcc ggacttcgca cccttgttca gtatgaagaa
     1261 gaaccggggt tcttcgtgtt ccgttgttcc cataattcgt aaagttagat gtttatttat
     1321 tccccttttg ttcccttgcc tgctcttggt agtattcata atactgcttg tagttgtccc
     1381 ggtctttggc tgcatccgtc caaagatagc acatgaaaca agcgaacaat agggagataa
     1441 cagcacaaag gattgagtag aacgaaattc gtttgtccac ataaccgccg tttttcattc
     1501 tgtacccctg ccgttcaaag tccgccaaca cgttttttag gcgattttcg gcactttcaa
     1561 ggcttttccc gtctatttgt acctttcggg ctgttgcgtc ctctatgcgc ttcgtataga
     1621 cttcaacacg ctttagttct tgatacacca gttctaccgc aagctgcggg tttacccttg
     1681 gcgttgtatc ttttcttttc cgggggctgt actgtccctg cttaacgttg gaagctgccg
     1741 cttccgggtg tgagtttgtt tccatgagat atgttcattg agttgcactt cagtttgctt
     1801 tgtgtccgct cgatatgggc gagagtgtag cctttcccta tctcgcttgc cttatactcc
     1861 gtaccgcttc gggctgtgac atagtagccg ttaagtttcc cggtgcttgc cctggcttcc
     1921 cggactttaa aacctctctt accaagttct tccttgaatt tggtaaagtc aaagccctgc
     1981 atcttcttca atacgcttct tcaatacgcc gtccatggct tccttgattt ccgctttgtt
     2041 gactttgcca atgtcctgcg actgtacaaa gttccgttcc ttggctatgg cattggcggc
     2101 ttccgttgcc tttttcccta tccagttgtc ccggtacagt tccccggaaa gggaaacccg
     2161 gtttgccagt atgtgcaggt gggcttgctc tttcttgctc tccgtaccgc tgtgccggat
     2221 gatgatgtac tggtggttcg ccaaccccat gcgctgcatg aagtcgttac caagttccgc
     2281 ccagtccgca tccgtaaagg tggcgctttc ctctatcgag gggctgacct cgaaacgtag
     2341 gcagttgttt tttacgttcg gaaaatcaat gtgatacggt ttcatttcct gcaccatttc
     2401 cgcaccagta cacccataaa gttcatgccg ggctatctcg gtggctactg cctgcccgtt
     2461 tatctctttg gcgaggtcgt actccaatgc cgccacacca tgtgatatgc ttttcccttt
     2521 ccctatcatc gtgagagtat ttttttgagc tctgtaatta gtgcccggtt ctcctcgaac
     2581 acttcccggt actggcgacc accgaagtag ttgttcagcc gttggagcgt tcctttcaac
     2641 tgggctatgt cccggagaag ctgccgttcc tcctcggtgt acctctccct tgggcgtccc
     2701 ccaagggaca aactccggca gtattccgat acgcttagac tgcaccgggc tgcctgctcc
     2761 gcaagtgcgt ccttttcaag ttcggtacac cggaatgtaa tcttttcgtt ccgtcttagt
     2821 tccatgccac aaaggtaatc ttaaaatctt tttgagcaaa gcgagaaaag caagaattgt
     2881 ccgacaagga cacttcttgc tatatataca catgccgcat gggaatacat ccacgccaac
     2941 cgcccagcaa aaagccccgg cgtgttagaa aatcccccac agcctaacaa attctacccc
     3001 atttctaccc aaggggatta cacgccgcag aagtcatccg ggaacatgtc ttccagttca
     3061 tccaaatcaa tggtttcttc ttcggacacc tccacccatt caatgttttc tttccctctt
     3121 atcgcacatg agtacatctc ggcggcggca agagaagaga acacctcgtg cataggctcg
     3181 ccgttctcta accagtaaac acaatattcg accataacag ttttatcatt tatcgttgta
     3241 atgcccctct acctccaaga tgtagacctc taccacttcc tcgtaaatgt catagattat
     3301 ccggtcatgg gcggtaatgt gtcgagacca tgtaatatcc ccaccgcccc ttaacgcttc
     3361 gggatgccca agcccagtct tagggtgctc taacaattct ttgtagattt tcttatactt
     3421 cttgaacaaa ttggggtttg atttcttcca tttggcaatc accttatcca ccccgtcgga
     3481 aaccctaatt gtgtacatca tagactatcg aaataagcgt caatatcctc actgcttttg
     3541 agggtaacac actttccgga tttgatttct tcccttgcct tgtcaatcct tgcttgcagc
     3601 tccggggtta tcatcaaatc ttcacgacca acttttacca aagcgtaaat ctcgtttttc
     3661 ctgcgaatta gtacctgctc gccattgtca gctttggtga aagacgctgc gaggttgtta
     3721 cggtattctc ttactgataa tgcttccata tctgtttgct tttaaattca gcacaaagat
     3781 agctatattt caataaaata caaacatttt gtacacaaac gtgtacacgc cataaaaacc
     3841 cgtttccaat cctaccgccc gttggttggt tttgctttgc tctttttccc tatcgttttt
     3901 ctttttccga cagtaaatca accacttagc tctataaatt ccccttgtct gttataagca
     3961 tcgtctgtaa tatgctctat cacttcttcg caaacgccta taaataaagg gattagaaca
     4021 tgtgtttccc cacactttgg gagtttagtc cccacacttt gggagtttaa aagtaaatta
     4081 gtccccacac tttgggagtt ttacccctct tttttgtttt aatgtgggga ttgtgtatat
     4141 ttgcagcaaa tcaatcgttt
//
