LOCUS       NZ_CP102992             4167 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KPA2 plasmid p5, complete sequence.
ACCESSION   NZ_CP102992
VERSION     NZ_CP102992.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30381623
            Assembly: GCF_024750555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPA2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/13/2023 03:43:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,665
            CDSs (total)                      :: 5,541
            Genes (coding)                    :: 5,385
            CDSs (with protein)               :: 5,385
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 63 of 156
            Pseudo Genes (incomplete)         :: 98 of 156
            Pseudo Genes (internal stop)      :: 25 of 156
            Pseudo Genes (multiple problems)  :: 24 of 156
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102992.1.
            Bacteria and source DNA available from Dog.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPA2"
                     /isolation_source="Small Animal Hospital, Chulalongkorn
                     University"
                     /host="Dog"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="15.8700 N 100.9925 E"
                     /collection_date="2020-09-01"
                     /collected_by="Small Animal Hospital, Chulalongkorn
                     University"
     gene            complement(join(3990..4167,1..65))
                     /locus_tag="NW253_RS28245"
                     /old_locus_tag="NW253_28245"
     CDS             complement(join(3990..4167,1..65))
                     /locus_tag="NW253_RS28245"
                     /old_locus_tag="NW253_28245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(431..1063)
                     /locus_tag="NW253_RS28250"
                     /old_locus_tag="NW253_28250"
     CDS             complement(431..1063)
                     /locus_tag="NW253_RS28250"
                     /old_locus_tag="NW253_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1063..2178)
                     /locus_tag="NW253_RS28255"
                     /old_locus_tag="NW253_28255"
     CDS             complement(1063..2178)
                     /locus_tag="NW253_RS28255"
                     /old_locus_tag="NW253_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2523..2636)
                     /locus_tag="NW253_RS28260"
                     /old_locus_tag="NW253_28260"
                     /pseudo=""
     CDS             complement(2523..2636)
                     /locus_tag="NW253_RS28260"
                     /old_locus_tag="NW253_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3052..3243
                     /locus_tag="NW253_RS28265"
                     /old_locus_tag="NW253_28265"
     CDS             3052..3243
                     /locus_tag="NW253_RS28265"
                     /old_locus_tag="NW253_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3243..3644
                     /locus_tag="NW253_RS28270"
                     /old_locus_tag="NW253_28270"
     CDS             3243..3644
                     /locus_tag="NW253_RS28270"
                     /old_locus_tag="NW253_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2524..2931)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gaggagaaga acgccggaaa caggacttcc cctggctgaa tggtgtgaat ttccgcgcta
       61 tgcacttgcg cgcatactca tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt
      121 tttcgggtgg tttgttgcct gttttaccgg tttcccgtca gaaacgtcct gagtccgttt
      181 tagcggtgcg tacaattatg attttatgtt aaattgtttt aagtgaatag agagaatata
      241 acttaaggga gcatcttgtg ctgggtcttt tacattagat ctgcacaatc attgttttgg
      301 catctggtga tgtctttagg acgtttgaga ttgctgataa ctaaagtgta tgatgctttc
      361 tatacgttat ccggcgttta ataacaccct ggtgataagc cagcccgtga agggctggct
      421 tgatttggat ttatattttt gaaataaaat cttttatttc atcaaagcaa ttgtctgctg
      481 tgtttattat tccatttttc atggatattc ctattcttga gtcggattct ctattgcagc
      541 taagatagac ttgtacctta gctttttttt cgtttgttgg ataagtgtac tctttgtcac
      601 tcgctgagat gtcatcttta ttatttttaa ttttttcgaa aaattctatt atgtattcat
      661 ataacgctcg agtgcttgcg tgttcaatta tcagatcttc aatggcacct tgcttgtctt
      721 ttcctggcat tataaagaaa gaaatttttg tattgccatt catagctatc tcgttatgcg
      781 agctggggga ggagatttgt agttctcgca atgtggcaat tattgatctt tctgtgtcag
      841 aaaatgatgt gtcagcatct aatataataa aaatactttc tttttcatcg aaagaatcaa
      901 tgtttttgaa tgctctaagt gcttgtttta attttgattt tccatttatt ttttcgacaa
      961 aaacatcggt aatgccttgt atttcaagta ggttgcatag aaaatgaaaa tcatcatcac
     1021 cctcaacgaa aatgatcttt ttgctattat ttatgtcttt cattatctta cctcccagtc
     1081 gttctcaact gagtaggaga aatcatccat attgaattgt acggctgttg gttttaggga
     1141 gttcttttct cttcctagtc taatataagc gatatcttca tttttgctat ctatttcgtt
     1201 tattgctcgt atcacatctt gactatgagt tgttgcaaat atttgattgt tgttttgttt
     1261 tgcagatgaa attaatgatt ttattatgtc tttaattact gagtaatgta ttccgttctc
     1321 gatttcatca ataagaataa ttgagttttc ctgtaccagc actgagctga taaaagataa
     1381 tgctcgactg ataccctcgc caagcattga tatttcattt agctcactaa atccaatgtc
     1441 taaatatatt tccttattgt tacctatcgc agatattgct atatctacaa ttctgtcatc
     1501 tatttttttt aagttttcta gtatgtcttt tttccttttt tgtgttagta ggctagatac
     1561 atttgtaatt gttgcctctt tgtttatctt tctggatgtt gttattattg ttgctgtctt
     1621 aaatttataa ttaatcccat ccctttcgtt ttttatttcg gattttattt ctacgccatc
     1681 ttctttcaat tgcatagaga aaagcttatt ggaaaccctg tttttcgcat tgggtgtctc
     1741 ggtcacattt atttttaaag agtttactgt ggcctgagaa ccgatagttt gacgaggtat
     1801 gttttggtta gatagtacgg tgtcaacgtt tagagacaaa cttgattcta atctctgatt
     1861 tgtttctgtt tcatatgttt gtgttacttc agagcgttca tctcgaatga ctatggacat
     1921 cttttgtgat gtgtccatat catgaaagta actaacccaa aatggcttgt ttttgtttat
     1981 ttctgccatt tcttttctga atgcttgaat gtttaacaaa acagccgggt tttttacatc
     2041 ataaaaacaa aaaatagcat ccagtataga tgttttcccg tagttgtttt tcccagctat
     2101 gatatttaaa gtctttaatt gctttagata tagctcattg aagcatttga agttctttaa
     2161 acttatttcc tttatcatga tagcctctgt tcgtgttttt gttatatgcc atacgatttg
     2221 ttagtacctt aataaaatgt acagctttca ttgtgattag ctagacttta tgtcaaagat
     2281 taactttgat tgcttgtgtt ttaatcgatt ttttttactt tcttcttgag attatctggc
     2341 agcagccgta agcttccggc tgtgaacgaa aaaaaccgcc ttggcgggcg gttttttcga
     2401 aaggttcagt agagttggcg ctctatgaac cgaggtaact ggcttaaagg gagcgcagtc
     2461 accaaaactg ttcgtttagt ttagctttaa ctggcgtata acttcaagac taactcccta
     2521 aatcagttac cagtggctgc tgccagtggc gctttgtcgt gtctttccgg gttggactca
     2581 agacgatagt taccggatta ggcgcagcag tcggactgaa cggggggttc gtgcatacag
     2641 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg
     2701 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     2761 ggagccatca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
     2821 atttgagcgt cagattccgt gatgctcgtc aggggggcgg agcctatgga aaaacggccg
     2881 ctgtgcggcc tttccttctt tcctccatgc gattactcgg ttttctcgag cgtttctacg
     2941 catgctcctg ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga
     3001 agcggaaaag aggagatccc tacactgacc actgacgctc tgaggcctct tatgacgaaa
     3061 caacagaaaa cagcgctgaa catggctaaa ttcatccaga atcagtctct gctcctgctg
     3121 gagaagctta acgagcttga tctcgatgtc gaggccgacc tttgtgagaa actccacgat
     3181 gatgcggagc atcttttccg tacactgtcc tcgagactgg atgcattaca ggatggtaac
     3241 tgatggccag agtgtctatt agtgaggctg cccgactgac gggtaagagt agaacaacac
     3301 tgcacaggtt aattaaagca ggtgacctgt ccacctgtag cggtgagcgc aatgcaaaaa
     3361 tggtagatac ttcagagctt ttacgtgtct ttggcccctt tgagcaaccg aagggtgaac
     3421 accattctgg acaggtctct ggacagcgtg atacagcctc tgctgaccag agtgaacagg
     3481 tgatccgtca tctgaaacag gagattgaac acctgaacac cttacttttg tccaaggatt
     3541 cgcacattga cagcctgaaa caggccatgc tccttcttga aaatcaccag gcaaaattaa
     3601 ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc ctgaccccgt catgcttcag
     3661 ttttcctctc cggccatccc agggagttcc tgaaccttcc caaaaatcgt cagatctctg
     3721 aacgctcctc accactaaaa atacccacac cccacgcaaa attaaaattt tgcagattcc
     3781 ttatttacaa caatgggtta atgaaaaata aatgtgcggt gatgtgttac aaagtgcaga
     3841 tccaagtcgt ggacgactct atggcgaatc gctgacgaat tgcccacgat tcatatgtga
     3901 atcacgcttt attatcatat tgttatgatg aatcgactct tgattcaatt catcactttg
     3961 ggtgtttttt gctcatggtg actcttgatt caattcacga ttcgaatcgc cggtgaatcg
     4021 tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcgac gaacccgatt
     4081 cctgaaggtg ctgaaaaccg gttttccggc tgcgccggca acgcttcacg gccacgcaat
     4141 aaattgcgtc cccgttcacc gttttcg
//
