LOCUS       NZ_CP102926             4783 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain K18-45 plasmid pK18-45_P5,
            complete sequence.
ACCESSION   NZ_CP102926
VERSION     NZ_CP102926.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30333744
            Assembly: GCF_024734295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Yu,R., Chen,Z., Li,L. and Du,X.
  TITLE     Study on tetA variant-mediated phenotype of high tigecycline
            resistance in clinical Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Yu,R., Chen,Z., Li,L. and Du,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) College of Animal Science and Veterinary
            Medicine, Henan Agricultural University, No. 15, Longzihu University
            Campus, Zhengdong New District, Zhengzhou 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:09:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,460
            CDSs (total)                      :: 5,337
            Genes (coding)                    :: 5,162
            CDSs (with protein)               :: 5,162
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 55 of 175
            Pseudo Genes (incomplete)         :: 122 of 175
            Pseudo Genes (internal stop)      :: 32 of 175
            Pseudo Genes (multiple problems)  :: 28 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102926.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K18-45"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="pK18-45_P5"
                     /geo_loc_name="China:Henan"
                     /collection_date="2018"
                     /collected_by="The First Affiliated Hospital of Zhengzhou
                     University"
     gene            join(4748..4783,1..207)
                     /locus_tag="NWF33_RS27175"
                     /old_locus_tag="NWF33_27175"
     CDS             join(4748..4783,1..207)
                     /locus_tag="NWF33_RS27175"
                     /old_locus_tag="NWF33_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(878..1093)
                     /locus_tag="NWF33_RS27180"
                     /old_locus_tag="NWF33_27180"
     CDS             complement(878..1093)
                     /locus_tag="NWF33_RS27180"
                     /old_locus_tag="NWF33_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1100..1591)
                     /locus_tag="NWF33_RS27185"
                     /old_locus_tag="NWF33_27185"
     CDS             complement(1100..1591)
                     /locus_tag="NWF33_RS27185"
                     /old_locus_tag="NWF33_27185"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1626..2330)
                     /locus_tag="NWF33_RS27325"
                     /pseudo=""
     CDS             complement(<1626..2330)
                     /locus_tag="NWF33_RS27325"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2269..2592)
                     /locus_tag="NWF33_RS27195"
                     /old_locus_tag="NWF33_27195"
     CDS             complement(2269..2592)
                     /locus_tag="NWF33_RS27195"
                     /old_locus_tag="NWF33_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_182481769.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRSLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3243..>3365
                     /locus_tag="NWF33_RS27330"
                     /pseudo=""
     CDS             3243..>3365
                     /locus_tag="NWF33_RS27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3685..4041)
                     /locus_tag="NWF33_RS27200"
                     /old_locus_tag="NWF33_27200"
     CDS             complement(3685..4041)
                     /locus_tag="NWF33_RS27200"
                     /old_locus_tag="NWF33_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(4244..4561)
                     /locus_tag="NWF33_RS27205"
                     /old_locus_tag="NWF33_27205"
     CDS             complement(4244..4561)
                     /locus_tag="NWF33_RS27205"
                     /old_locus_tag="NWF33_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2708..2881)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          2805..3352
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(787..2279)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2276..2592)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="7.5e-60"
ORIGIN
        1 tttgaagcag atacgaagtt taactcggtt tttgacactg aatataagcc gggagaaaca
       61 tgtgaatatg aagggattta taaatgtacc ggttgcggta aagaagtaac tatcgcaggc
      121 gggaaaacaa tcccaccaca aaaccaccac caacataccc ccgcgcaagg gaacattcgt
      181 tggcaattgt ttgtttatgc tcagtgaggt atagaaaatg gcagatcgtc gaccactcaa
      241 cccacacgtt gtcgttccta aaccaggcgg cggaactcaa cctcgtgacc gtaacaatga
      301 cggaagggtc agaggaaaac gctcagatgc aggaaagcca cgaaaataac agcaagccgc
      361 cagcctggcg gcttttttat atctgcagct gatactgatt taccataaca tccgttgggc
      421 gcaccgctga aacggggttg gctgcgtttc cggtgccaga cgcgagaaag tggcgacaga
      481 cataacgaaa aaacaccaga aacgcagccg ctgaatatta cactggtgca tagtcatgca
      541 gccagatgca tggatgttaa agtcacacta aaatccgcgc caatgcctta aaaaccgaca
      601 aatgcttctc cagccagtcc cacagctccg ccgccagata caggcagtta agcaatgatt
      661 caatgagatt taacagcttc atttcccgct cctgaataag tcgtgacctt tttgatcacg
      721 aacctattta gcgggagaaa taagagcccc tcttcgtgcg ggttgtcagc tgcctggatg
      781 gtttaaagtg tcggcccgcg gtaggttttc tgcggctggc gctccagctc cagtgttttc
      841 tgatactcat tctccctctg ccggagctcg atggcattca gttcccggac aagcggcgca
      901 gctgctcgct taaactcagc acctgcgtgc tcaagtccga cacgcgctcg ttcagcaccg
      961 cgttctcccg ttgcataagc ccacacatgc tccgccattc cgcgaaggcg ctctcccatt
     1021 cgtccagcct ttgcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgctttt
     1081 ccagctctgt cattttccgc tactcctgtt tcaggcggat ccatgtcgtc ccgtcgtagg
     1141 ggatggcttc ggtttgcatc ccgggcggca ggataagaaa cctccggccg tcgctgctct
     1201 cctgatagcg cactccccac gtccgggcag tcattttcgc cagggtatct tcctgctgac
     1261 gaagatgcgt gtaattgtcg gttatctgct gcccctgcca ccacagtatg ctcccgctcg
     1321 taccgatcag cagcgctgac accatcagga tggtgaacca tgtccgcccg gcagtccgca
     1381 gtacgctccg ccggtttttc tccatcgcct cgctcatact ctgctcgtgt gcgaggatgg
     1441 cgtcgctgat tctcctcgcg cttgcgctca gttccgctct gacagactgc tcgtgctcgc
     1501 tgaatgcggc tttcagcatc tcgccggtac tctgctgctg cgctttcgat tgctgctcta
     1561 agtccttcgc cagcgttaaa agactgctca tagatggctc ccttcagtcg gatgtttcgc
     1621 cccccgtccg ggtcggcaat gctgatactg cttttcgtgg tgcgcaccac ctcaaaacct
     1681 gaccgttcca gcgcctcagt gacgtcctga cggtttttca gctccccgga cgaagcaagg
     1741 gtcagcagtc cgcgcgtgat ggcttcagtg gcctcctgct tcgttttcgg cagtccggac
     1801 ggcgtgacca gcgcccggcg gttttccggc gcgttcgggt catgcagccc cagtctgccg
     1861 ttcacgatgg tctgccaggc atcgatgcgc ggccggtcgg cgcggtcgta gtacggctgg
     1921 agccgcctgc cggtcagcag ttcggtattg gggatcagaa agttcagctc cagacgcccc
     1981 ttgtccgcgt gctccaccca cagaatgctg tactgatctt tatcgagtcc gggcatcaga
     2041 acccgctcga agctggccat cagtttttcc cgctggccgg gaggcagatc ggcctccgca
     2101 aacgacagta cgccggaggt gtatttcttc acgtaaggcg acgcgtcaat cagctcccgg
     2161 acttcttccg gcttcccctg caggacgctg gcgccttcac gctgccggtc tttacccagc
     2221 agatagtcca ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt aacgatcatc
     2281 gtccgttcct ttctcaagaa cggcgtgccg cagccgctca agcccggcat cgatggccat
     2341 cagcgccgcc acaatctgta ccctgtcatg gccggtgccg ccgccggcgt taacacggcg
     2401 ggctatctgg ttcaggttat tgcccatacc ggcaagctgg cgcagcagcg ccggcgagag
     2461 tgaggggagc tttccggtgc gcgcaggctt ttcgtccagg caggtctgcc gcatccacgc
     2521 cgccagcgac ctgccgtcgc agcgctcaag cagccgccgg tgctcgccct ccgtcaccca
     2581 catggtgagc atcttgttcc gtttctctga caaaccggcc tcctgataac gccgggacgg
     2641 gcgggaaagc ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcagtggctt
     2701 ttggcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgta gtgtatactg
     2761 gcttaatatg ttggcacgga tacggaaatc agtgaagtgc ttcatgtggc aggagaaaaa
     2821 aggcggcaac ggcgcgtcag cagaatatgc gatacaggat atattccgct tcctcgctca
     2881 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt gcgggcttac gaacggggcg
     2941 gaaatttcct ggaagatgcc aggaagaaac ttaacaggga agcgataagg ccacggcaga
     3001 gccggttttc cataggctcc gcccccctga cgagcatcac agaatctgac gctcaaatca
     3061 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg atggctccct
     3121 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     3181 tttgtctcat tccacgcctg acactcggtt ccgggtaggc agttcgctcc aagctggact
     3241 gtatgcacga accccccgtt cagtccgacc gctgcgcctg ttccggtaac tatcgacttg
     3301 agtccaaccc ggaaagacac gacaaaacgc cactggcagc agccactggt aacaggatta
     3361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3421 acactggaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc aggttaagca
     3481 gttccccaac tgacttaacc ttcgaaaaac cgcctcccca ggcggttttt tcgtttacag
     3541 agcaagagat tacgcgtaca gagcacggat ctcaagaaga tcgtaagaag aaagttaaca
     3601 tgagcgtttt tatccctcaa aaagaaggct tctagttata gaagccttct ttttgaggga
     3661 taacttatat ttttttaaaa aaaatcatgc aatagcatgt ttcttctcag aactatcaac
     3721 aatgtgtttc ctttctatga tttcatgagc gggaatcact ctgtctattt cttcttgcaa
     3781 tcgagggcta ctagtaacct cccataaatc aacagactgc tgtatattta gccagaactt
     3841 tgcagtggta tcgaaggctt tagccaacct aaaagccata tcggtagaca gctttctatt
     3901 attgttaact aaagcactgg cagagtttct atgcacattc aacatttcag ccaagtcatt
     3961 gatttttaaa ccacttggtt cgagatattc ataaagaata atatccccta cagttgatgg
     4021 ttttctagtt gcttgggaca tgctttactc ctaaattttt tacttctctc tgttagatga
     4081 ggaagataag tatttcgtct attaccagct gtagcgatga gtaattatct taaatatagt
     4141 cacgtcattt tttttattaa tcattatttg taatgacatt ttaatgtagg tgagggaacg
     4201 cctcacctac ttgttttata agataacagc cacgacctgt tatctatcgg taatcatgtg
     4261 gatcgagata caaatcctcg actccgtttt caccccactt aaatatcaag cggtacttct
     4321 cattcacccg tatggatgaa taccctgtca atggagggtt gagattctca aatcggtttc
     4381 ccgggggaga ccgcaaatct ccaactttat cagcagcatt cagcatatcc agcttgcgac
     4441 ctaatgaagc actaatcgca gttggaatct ttcgattggc tttaccatat ttgtaaaact
     4501 ggtatagcca atcgtctcga aactgaatgc ggcaatcatc atgttcatga tcttgctgca
     4561 tttttctttt acctcaattt ttgccgcaac ttggcaattt tttcgatcac cttcttacct
     4621 ccgatatagt acagcaaaca tatcaaatgc acaacagcac agtgcacttg tgcaatcaat
     4681 ggctatgctg tagaatgcac tcatgcacag tgcaggtgtg caaaataaat gataatttgg
     4741 aggttgcatg gctcttgttc gctcaggaaa caaaagcaag att
//
