LOCUS       NZ_CP102925             4869 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain K18-45 plasmid pK18-45_P4,
            complete sequence.
ACCESSION   NZ_CP102925
VERSION     NZ_CP102925.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30333744
            Assembly: GCF_024734295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Yu,R., Chen,Z., Li,L. and Du,X.
  TITLE     Study on tetA variant-mediated phenotype of high tigecycline
            resistance in clinical Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Yu,R., Chen,Z., Li,L. and Du,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) College of Animal Science and Veterinary
            Medicine, Henan Agricultural University, No. 15, Longzihu University
            Campus, Zhengdong New District, Zhengzhou 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:09:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,460
            CDSs (total)                      :: 5,337
            Genes (coding)                    :: 5,162
            CDSs (with protein)               :: 5,162
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 55 of 175
            Pseudo Genes (incomplete)         :: 122 of 175
            Pseudo Genes (internal stop)      :: 32 of 175
            Pseudo Genes (multiple problems)  :: 28 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102925.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K18-45"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="pK18-45_P4"
                     /geo_loc_name="China:Henan"
                     /collection_date="2018"
                     /collected_by="The First Affiliated Hospital of Zhengzhou
                     University"
     gene            join(4379..4869,1..43)
                     /locus_tag="NWF33_RS27150"
                     /old_locus_tag="NWF33_27150"
     CDS             join(4379..4869,1..43)
                     /locus_tag="NWF33_RS27150"
                     /old_locus_tag="NWF33_27150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101866523.1"
                     /translation="MVRRKITVSDKTKGLFKPFFDLTLYIHEEGFEEEEYKRLVGLCFE
                     SMGKGKLSVNRPMSNGKGFLTFIYRIDSNLLLDEFINRFEKKITKKRYMDFLLFILTDD
                     TENIEKIEKMFTRICGVDIEDNVLKAIECLKKDSYTPERFDDLIIYFRTKKEKREIESI
                     MFDSNNNNKKRGRL"
                     /name="hypothetical protein"
     gene            40..342
                     /locus_tag="NWF33_RS27155"
                     /old_locus_tag="NWF33_27155"
     CDS             40..342
                     /locus_tag="NWF33_RS27155"
                     /old_locus_tag="NWF33_27155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101866522.1"
                     /translation="MRRTPEQRKAYLEAQLNSVKAKINKQKRKDYTVKKRETDHLKYTF
                     AGDILKVMGCDVREVDLSLILGAVFYATSENANPKNVEIYRKKGLEILKENKKEI"
                     /name="hypothetical protein"
     gene            1235..2677
                     /locus_tag="NWF33_RS27160"
                     /old_locus_tag="NWF33_27160"
     CDS             1235..2677
                     /locus_tag="NWF33_RS27160"
                     /old_locus_tag="NWF33_27160"
                     /inference="COORDINATES: protein motif:HMM:NF013603.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_258342310.1"
                     /translation="MFRDSVFNKNRSVNRPNNLKEFARVNGERITERKKETFNVALSMK
                     DKGEDLQDETLTKRAERSLKCSSFRYIATVNGQAQTLYTHRCKGRHCQECQRIKAYIWQ
                     KKTESIFPKLEENYLNAKYLFATFTIKNPKITDLRVVLSVMNKAANRLFKMKKYKDFIL
                     GGIRSTEITRGKSGADECHPHFHFLLMVKGNYGKKYYVNQEDWGKDWGDCLAYECDKAG
                     YPYNPIDYPRGFPIVDVVRAMDKEKKIEITNKNIKNSGEAIINYVLKYSVKGSDILDHK
                     KGKNDNWFFEFDKQIKGCRMITPVGIFKKYLSEIKKDPFDYEIEKAKIEERLGEGYQVN
                     KAIFKKGDYKLENKKDSEGYLLEALEAKAYNFQQLYLTIYSDYIKFYNDTNREISKLED
                     KLFNEDNMLIESKIGRLLLIQEDRKKRINNTIESLRRVGYLKEEKGLYYDEWGSLWATF
                     EIEKPNIEVVEINDFADMFEVW"
                     /name="protein rep"
     gene            2677..2823
                     /locus_tag="NWF33_RS27165"
                     /old_locus_tag="NWF33_27165"
     CDS             2677..2823
                     /locus_tag="NWF33_RS27165"
                     /old_locus_tag="NWF33_27165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_185226606.1"
                     /translation="MKKKQKPKKRNRNKKSKFFKLLMASVREAVEISKGNIEPARITIL
                     NKE"
                     /name="hypothetical protein"
     gene            complement(2858..4117)
                     /gene="mobQ"
                     /locus_tag="NWF33_RS27170"
                     /old_locus_tag="NWF33_27170"
     CDS             complement(2858..4117)
                     /gene="mobQ"
                     /locus_tag="NWF33_RS27170"
                     /old_locus_tag="NWF33_27170"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_258342307.1"
                     /translation="MAIYYFDVSNVSRSSGKKSASSVAASAYQNRARYTDERTGTTYDY
                     TKKGGLYYSGILAPENAPESLTASPLTLWNTIEGIEKRKDARLAKDFKISLPIELSPEQ
                     NKALILDFAKQAFVSKGMIVDLAIHDIDTQPHSHIKTTTRELTPEGFGGKVREWDKKET
                     TLYWRELWAKVANEHLLKAGHDITISHLTLKEQMKEALEEMDKTTDLEEKIKLASKAIE
                     LNRPAMKTISKKDWGDPKFQEIRKQEQEAKKQILKKAKNFRIKNTRELREIQKELDKKF
                     RPSKIYKSISEAIRNLNNKITDSKLNNILNDKLDKLTETIKSVPNKIKANIENRRLEKE
                     RDERIKKGIEEDKRKIREADKKWNEENNKLIKQQLARNKENSEAERQRLKLEDEANRLA
                     EKNNISPNDFKQKKKKGLNV"
                     /name="mobQ"
ORIGIN
        1 gtttgattca aataataata ataaaaagag gggtagatta tgagaagaac accagaacag
       61 agaaaagcat atttggaagc tcagcttaat agtgtaaaag caaagataaa caagcagaaa
      121 agaaaagatt acactgttaa gaaaagagaa acggatcatc taaaatatac ttttgcaggt
      181 gatattttaa aagttatggg gtgcgacgtt cgggaggttg acttgtcttt aatactcggt
      241 gccgtttttt atgctacaag tgaaaatgca aaccctaaaa atgtagaaat atatagaaaa
      301 aaaggattgg aaattttaaa agaaaataaa aaagaaattt gacaaaaaca taattagaat
      361 atatttaata tatagtcgtt tatatattta gtatcctttc ctgtggagag ttaagaaatt
      421 atttattaat atatatacgg ctaatttgcc ccatttatgg ggatattgga gaggctaact
      481 taggttagcc tttcgtcctt tctgatccag ttcaccagtg ccgaaccgtc aagcagaatg
      541 tcggggggtc ccctctgggg ttccgtcatt gtgcttgtac ggctccctgg tcaatacaac
      601 acttggcaaa ggtggaaggt gaacaaagtg agccttctac ctgataagca gcccttaaat
      661 ggttttaagt tgtattaaat gacatttgat aatacaactt gccccggtag gggttgcctt
      721 tggttttagg ggtttggttt gaggttgccg aaggcgaccc ctccccttgt ttagtaagaa
      781 acaaatggag gcaaaacttg gcttctaaaa ctcccctaca agccccgtca ttactggctt
      841 taaccccatt tttttagcag ttttcaaggg gggtaaatag cactttttgc taaagaattt
      901 tagaacaaaa aataatcaat cccccgaaaa tgggacagca cctttaagat cgccagaaaa
      961 caccccaaaa aatgggacga caccttttaa aactatccga caaacggaag atttgggacg
     1021 gaacatttag aacaccggtt ttttggtgtg aaaaatggga cggatctaac cgtaacggca
     1081 aaaaaacacg gaaaaaatgg gacggtatcg acttctggcg gtgggggaag ttttggaggt
     1141 ggatctggta agtgcttgac tgatttgaca aaaagaaaaa accccgtaaa gtaagaattg
     1201 ctgaggttct atacaatacg gggtatttct taatatgttc agggatagtg tatttaataa
     1261 aaataggtct gtcaatagac cgaataatct aaaagagttt gcccgtgtaa acggtgaacg
     1321 aataacagaa agaaaaaaag agacctttaa tgttgctttg tctatgaaag acaaaggcga
     1381 agatctacaa gacgagacat taactaaaag ggctgaaagg tctttaaagt gttcttcttt
     1441 tcgttatatc gccacagtta acggacaggc tcaaacctta tatactcacc gttgcaaagg
     1501 tcgccattgc caggaatgcc aaagaattaa ggcttatatc tggcagaaaa aaacagagtc
     1561 tatttttcca aaactggaag aaaattattt aaatgctaaa tatttatttg cgacatttac
     1621 gattaaaaac ccaaaaatta cagatctaag ggttgtttta tcggtaatga ataaagcagc
     1681 aaatagactt tttaaaatga aaaaatataa agattttatt cttggaggta tccgttcgac
     1741 ggagatcaca aggggaaaat ctggggctga cgaatgtcac ccacacttcc attttttgtt
     1801 aatggtaaaa gggaattacg gtaaaaaata ctatgtaaat caagaagatt gggggaaaga
     1861 ttggggggat tgcctggctt atgaatgtga taaggcaggg tatccgtata acccaatcga
     1921 ctatcctcgc gggttcccaa tcgtggacgt cgttagggct atggataaag aaaagaaaat
     1981 tgaaatcact aataagaata ttaaaaattc gggtgaagcg attataaatt atgttctaaa
     2041 atattccgta aaaggtagtg atattttaga tcataaaaaa gggaaaaatg ataattggtt
     2101 ttttgaattc gataagcaaa taaaaggctg tcgaatgata acaccggttg gaatttttaa
     2161 aaaatatcta agcgaaatta aaaaagatcc gtttgattat gagattgaaa aagcaaaaat
     2221 agaagaaaga ttgggggaag gttatcaggt taataaagct atatttaaaa aaggcgatta
     2281 taagcttgaa aataaaaaag atagtgaggg ttatttattg gaggcattgg aagcaaaagc
     2341 ctataatttc cagcaattat atttaaccat atatagtgat tatattaagt tctataatga
     2401 cactaacagg gaaatatcta aattagaaga taagttattt aatgaagata atatgttgat
     2461 cgagtctaaa ataggtagat tattattaat tcaggaagac aggaaaaaac gaattaacaa
     2521 tacaattgaa agcctaagga gggtaggcta tcttaaagaa gaaaaagggc tttattatga
     2581 cgaatgggga tcgctttggg caacattcga aattgaaaaa cctaatattg aagtcgttga
     2641 aattaatgat tttgctgata tgtttgaggt gtggtaatga aaaagaaaca gaagcctaaa
     2701 aaaagaaatc gcaataaaaa gagtaaattt tttaaattac taatggcttc agttcgtgaa
     2761 gccgtcgaaa tttcgaaagg aaacattgaa ccagcaagga tcacaatttt aaataaagag
     2821 tagtatagta tattgtagta tatagtagta tatagttcta tacatttaac cctttctttt
     2881 tcttttgctt aaagtcgtta ggtgaaatat tatttttttc agccagtctg ttagcctcgt
     2941 cttctaattt taacctctgt ctttcggctt cggaattttc tttatttctg gctaattgtt
     3001 gtttaattaa tttgttattt tcctcattcc attttttatc ggcctctctt atttttcttt
     3061 tatcctcttc aatccctttt tttattcttt cgtctctttc tttttccagt ctcctatttt
     3121 caatattggc ttttatttta ttaggaactg attttattgt ttctgttaat ttatctaatt
     3181 tatcatttag aatattgttt aattttgagt cggtgatttt attatttaaa tttctaattg
     3241 cctcggaaat tgatttatat atttttgacg gtctaaattt tttatctaat tctttttgta
     3301 tttctcttaa ttctctggtg tttttaattc ggaagttttt agcctttttt aatatttgtt
     3361 tttttgcctc ctgttcctgt tttcttattt cctgaaattt gggatcgccc cagtcttttt
     3421 tgctgattgt tttcattgct ggtctattta attcgatagc ctttgaagca agttttattt
     3481 tttcctccag gtctgttgtt ttgtccattt cctccagtgc ttctttcatt tgttctttta
     3541 atgttaaatg gctaatggtt atatcatgcc cagcctttaa taaatgttcg ttcgccactt
     3601 ttgcccataa ttccctccag tataaagtcg tttctttttt atcccactcc ctaacttttc
     3661 caccaaatcc ctccggtgtt aattctctgg tagttgtttt aatatggcta tgaggttggg
     3721 tgtctatatc gtgaatagcc agatctacaa tcataccctt tgatacaaaa gcctgttttg
     3781 caaagtctaa aattaaggct ttgttctgtt ctggggatag ctctatgggt agggaaattt
     3841 taaagtcttt ggcaagcctg gcatcctttc ttttctcaat accctctatg gtgttccata
     3901 gggtcaacgg tgaagctgta aggctctcag gggcattctc aggggctaaa atgcctgaat
     3961 agtataaccc ccccttcttt gtgtagtcgt aggttgtgcc tgttcgttcg tctgtgtatc
     4021 tggctctgtt ctggtatgca gaagcagcaa cagacgaagc cgattttttg cctgaacttc
     4081 tgcttacatt ggaaacatca aaataatata tagccatatt gtaaaacctc cacttttcta
     4141 atttttttgg ggtttggttt ttgcttttct tttttcccgt ttacggggaa aaaccctgtc
     4201 attgcgttag caatgcgtag aagggcttca agtaattttt ctttttcgta aactcaaaat
     4261 aaaaaacttt cagaacggca aaaatgcttt tccttatttt agatataaca taaatttgac
     4321 taaagtaaat tgtggaggta tatggttatt aattaatcta aatataatgg aggtttttat
     4381 ggtaaggaga aaaataacag tatcagataa aacaaaggga ctatttaaac ctttttttga
     4441 tttaacttta tatatacacg aggaaggctt cgaagaagaa gaatataaaa gactcgttgg
     4501 gttgtgtttt gaaagtatgg gaaaaggaaa actaagcgta aatagaccaa tgtcaaacgg
     4561 caaaggattt ttaactttta tatatagaat tgatagtaat ttattgttag acgagtttat
     4621 aaatagattt gagaagaaaa taactaaaaa acgatatatg gattttttgc tgtttatttt
     4681 gacagacgac accgaaaaca tagaaaaaat agaaaaaatg tttactcgaa tttgtggggt
     4741 agatattgag gataatgttt taaaggctat tgaatgttta aaaaaggaca gttacacacc
     4801 tgagagattt gacgatttaa ttatttattt taggactaaa aaagaaaaaa gagaaattga
     4861 gagcattat
//
