LOCUS       NZ_CP102908             4353 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain KW4 plasmid pKW4-5, complete
            sequence.
ACCESSION   NZ_CP102908
VERSION     NZ_CP102908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054364
            Assembly: GCF_024734275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 326.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:11:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,295
            CDSs (total)                      :: 5,172
            Genes (coding)                    :: 5,033
            CDSs (with protein)               :: 5,033
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 50 of 139
            Pseudo Genes (incomplete)         :: 89 of 139
            Pseudo Genes (internal stop)      :: 24 of 139
            Pseudo Genes (multiple problems)  :: 22 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102908.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KW4"
                     /isolation_source="well water"
                     /db_xref="taxon:1463165"
                     /plasmid="pKW4-5"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            complement(19..420)
                     /locus_tag="NWT71_RS26410"
                     /old_locus_tag="NWT71_26410"
     CDS             complement(19..420)
                     /locus_tag="NWT71_RS26410"
                     /old_locus_tag="NWT71_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040242827.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGARNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(420..611)
                     /locus_tag="NWT71_RS26415"
                     /old_locus_tag="NWT71_26415"
     CDS             complement(420..611)
                     /locus_tag="NWT71_RS26415"
                     /old_locus_tag="NWT71_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040242829.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDDN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1497..1772
                     /locus_tag="NWT71_RS26420"
                     /old_locus_tag="NWT71_26420"
     CDS             1497..1772
                     /locus_tag="NWT71_RS26420"
                     /old_locus_tag="NWT71_26420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040242819.1"
                     /translation="MSKGGALAKRAKTQNDSHGGRDLIIRKMEPSFPFAIRSIQADGII
                     ILDFIGDEISPNKREAFASVLLTKTIAQDLKSKIDGYLAHQENNEE"
                     /name="hypothetical protein"
     gene            1775..2488
                     /locus_tag="NWT71_RS26425"
                     /old_locus_tag="NWT71_26425"
     CDS             1775..2488
                     /locus_tag="NWT71_RS26425"
                     /old_locus_tag="NWT71_26425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040242822.1"
                     /translation="MDVQPTYYTQDAEMHEIVINIRKPSTEKRGEGKSSIFYTKTSSVS
                     KTIETKSYTTHLKPFIEKKDNEEHLWLKNLEQKAKLAAELISKGESNTDIIGVITSSLN
                     QNKTLVEDLKNAIEAHKESMKNVSDKNNESEIKGPNISLLSLFNALKFYPVISTLPKEM
                     VSQYIDADAHIVGMVIGDNNNKININFMSTGELLFRHIDKTTGRVRISGSAFFGKDENI
                     KDMKKIKKLISFVKI"
                     /name="hypothetical protein"
     gene            2501..3043
                     /locus_tag="NWT71_RS26430"
                     /old_locus_tag="NWT71_26430"
     CDS             2501..3043
                     /locus_tag="NWT71_RS26430"
                     /old_locus_tag="NWT71_26430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_135712390.1"
                     /translation="MFSAILNTSKNSNAEKYKFLSLRIWRLVTVKTENSTTPKGFIDGV
                     LTRNAFDLRPNEKSVSFSRLETEESIPNDSLKNKKLMACKIRTANKMQNIFISEESANN
                     EDLRLDPETNELLKHISFSETENSKFSNDGITGFHWDLFFISKNGNKLRSGDSELSEKH
                     ILDAKDLLIEMSLLPNS"
                     /name="hypothetical protein"
     gene            3320..3529
                     /locus_tag="NWT71_RS26435"
                     /old_locus_tag="NWT71_26435"
     CDS             3320..3529
                     /locus_tag="NWT71_RS26435"
                     /old_locus_tag="NWT71_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_040242824.1"
                     /translation="MKSIKNFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     Typing          733..1139
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.311"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aactgaagca tgacggggtc aggatttttt ccagaatttc caccatggcg ccggggattc
       61 cgttaatttt gcctggtgat tttcaagaag gagcatggcc tgtttcaggc tgtcaatgtg
      121 cgaatccttg gacaaaagta aggtgttcag gtgttcaatc tcctgtttca gatgacgaat
      181 cacctgttca ctctggtcag cagaggctgt atcacgctgt tcagagacct gtccagaatg
      241 gtgttcaccc ttcggttgct caaagggacc aaagacacgt aaaagctctg aagtatctac
      301 catttttgca ttacgtgcac cgctacaggt ggacaggtca cctgctttaa ttaacctgtg
      361 cagtgttgtt ctactcttac ccgtcagtcg ggcagcctca ctaatagaca ctctggccat
      421 cagttatcat cctgtaatgc atccagtctc gaggacagtg tacggaaaag atgctccgca
      481 tcatcgtgga gtttctcaca aaggtcggcc tcgacatcaa gatcaagctc gttaagcttc
      541 tccagcagga gcagagactg attctggatg aatttagcca tgttcagcgc tgttttctgt
      601 tgtttcgtca taagaggcct cagagcgtca gtgctcagtg tagggatgtt ctcttttccg
      661 cttcctcgct cactcagtcg ctatgctcct gccgttcggc tgcggcgagc ggcaggagca
      721 tgcgtataaa agctcgagaa aaccgagcgg tcgcgtgacg gggaaaagga aaggccgcta
      781 agcggccgtt tttccatagg ctccgccccc ctgacaagca tcacaaagct gacgctcaaa
      841 tcagtggtgg cgaaacccga caggactata aagatatcag gcgtttcccc ctgatggctc
      901 cctcgtgcgc tctcctgttc ctgccattcg gtttaccggt gtcattccgc tgttatggcc
      961 gcgtttatct cattccacgc ctgacactca gttccgggaa ggcagttcgc tccaagctgg
     1021 actgtatgca cgaacccccc gttcagtccg actgctgcgc cttatccggt aactatcgtc
     1081 ttgagtccaa cccggtaaga catgcaaaag cgccactggc agcagccact ggtaactgat
     1141 ttagaggagt tagtcttgaa gttatgcgcc agttaaggct aaactgaaag aacaagtttt
     1201 ggtgactgtg ctcctccaag ccagttacct tggttcagaa acttgccggt tactgaacct
     1261 tcgaaaaacc accttgccgg gtggtttttt cgttttctgc ataaaagatt acgatgagaa
     1321 gggaaggatc tcaagaagat cctttacttt acacatataa cattaacctt tcataccaga
     1381 aaggagcacc tttcaagctg aggtactata aatctacatg taagttatga tagataaggt
     1441 ataacatcac tagatatgtt tatcagtatg atgacaactc aaaaaaagga caaaaaatga
     1501 gcaaaggtgg ggcactggct aaacgtgcaa aaacacaaaa cgatagccat ggcgggcgag
     1561 atttaataat aagaaaaatg gagccatctt ttccatttgc tattcgaagc atccaagctg
     1621 atggtatcat catattagac tttattggtg atgaaatatc cccaaataaa agagaggcct
     1681 ttgcgtccgt tttattaaca aaaaccattg ctcaagactt aaagtctaaa atagatgggt
     1741 atttggctca tcaagaaaat aacgaggagt aaatatggac gtgcaaccaa catattacac
     1801 tcaagatgca gagatgcacg aaatcgtgat caatatcaga aagcctagca ctgaaaaaag
     1861 aggtgaaggt aaaagttcta ttttttatac aaagacatca tctgtatcta agaccattga
     1921 aactaaatcc tacactactc atcttaaacc attcattgaa aaaaaagata atgaagaaca
     1981 cttatggctt aaaaatctcg aacaaaaagc aaaattagcc gcagaattaa tatccaaagg
     2041 agaaagcaac acagatatca taggagtaat tacttcaagt ttaaatcaaa ataaaacact
     2101 cgttgaagac ttaaagaacg ccattgaagc tcataaagag tcgatgaaaa atgttagtga
     2161 caaaaataat gaaagtgaaa taaaaggccc taatataagt ttactatctc tattcaacgc
     2221 actcaaattc tatcctgtaa tatcaacatt gccaaaagaa atggtatctc aatacattga
     2281 tgcagatgcg cacattgttg ggatggttat tggagacaat aataacaaga taaatataaa
     2341 ctttatgtct acaggagagc ttttatttag gcacattgac aaaacaacag gtagagtacg
     2401 cataagcggg agtgcttttt ttggaaaaga tgaaaacata aaagacatga aaaaaataaa
     2461 aaaactcatt tccttcgtaa aaatataaga gatagaacta atgttttctg ccattttgaa
     2521 tactagcaaa aacagcaatg cagagaaata caaattcctt tctctcagaa tatggcgttt
     2581 agttaccgta aaaacagaaa atagcaccac tccaaaagga tttatcgatg gcgtgttaac
     2641 cagaaatgca tttgacctcc gcccaaatga aaaatcagta tctttttcac gccttgagac
     2701 tgaagagagc ataccaaatg actcgttaaa aaacaaaaaa ctcatggcat gcaagattag
     2761 aacagcaaat aaaatgcaaa atatcttcat aagtgaagaa tctgcaaaca atgaagattt
     2821 gagattagat ccagaaacaa acgaactgct taaacacatc tctttttcag aaacagaaaa
     2881 tagtaagttt tcgaatgatg gtataactgg ttttcattgg gacttgtttt ttatatctaa
     2941 aaatggcaat aagttgcgct ctggggatag cgagttgtct gaaaaacaca tattagatgc
     3001 aaaggatttg ttgattgaaa tgtcattact accaaattcg taataacctc cacttagtcg
     3061 caggaatagc caacattagg aataaataca agaaggtaat ttaacatcct cctctaattg
     3121 tacgaactct cgatgctctt cattctgttc gctgaaacca gaggctcaca cttgttttga
     3181 cgcgcttaat ttttattctg tttttttgaa taacatccgc aacttttttc gattttaatc
     3241 agatcatggt cgtcctatca tttatcacat catggcaaca acgccaaata cagaacaaaa
     3301 taacttaatg aatacgacca tgaaatccat caaaaatttc gttgcagttg ccgctctatc
     3361 catgatgtct ttcggtgcct ttgctcagag cgtcagcgct accgcctcca ccctggatcg
     3421 tgctgaagca aaaattgccg cacaggccgc tgaacaaggt gcgtcataca aaatcaccag
     3481 cgctcagttc aataaccgtg tccacatgac ggctgaactg accaaataag atggtcgcag
     3541 agggagctgc cgaacaggtc gccgggaggc ggcccgtttc gtcccttagc agaaaaacct
     3601 ccaggcccct tcgggggctt ttttgcatct gtcatttaac ataaaggcat gattgtacgc
     3661 accgctaaaa cggcctcagg gcgtttctga cgggaaaccg gtaaaacagg caacaaacca
     3721 cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg gcatgcatga gtatgcacgc
     3781 aagtgcatag cgcgaaaatt cacgccattc agccagggta agtcctgttt ccggcgttct
     3841 tcccatccga aaacggtgaa cggggacgca atttattgcg tggccgtgaa gcgttgccgg
     3901 cgcagccgga aaaccggttt tcagcacgtt caggaatcgt gttcgtcgac ggccggcaat
     3961 cacgttatgg cgagtcgccg gacgactcac cacacgattc accggcgact cttcactaga
     4021 gtcgtaccta gagtcgccga gtctaaaaaa caaccaaacg gggagtcggg tctagagtcg
     4081 ggtcgttatt aactcattgt ttttatagcc ctgagtcgtt accgactcta tgacgactcg
     4141 ccggggactc tatgacgact cgtcatagag tcggatctat gtaaataaaa tttaaaaacc
     4201 gaaatttcag gaaagtaagc tactgatatt aaatacaaat ctgcaaaatt ttaattttgc
     4261 gtagtgtgtg ggtattttta gtcgtgagga gcgttcagag atctgacgat ttttgggaag
     4321 gttcaggaac tccccgggat ggccggagag gaa
//
