LOCUS       NZ_CP102907             5207 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain KW4 plasmid pKW4-4, complete
            sequence.
ACCESSION   NZ_CP102907
VERSION     NZ_CP102907.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054364
            Assembly: GCF_024734275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 326.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:11:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,295
            CDSs (total)                      :: 5,172
            Genes (coding)                    :: 5,033
            CDSs (with protein)               :: 5,033
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 50 of 139
            Pseudo Genes (incomplete)         :: 89 of 139
            Pseudo Genes (internal stop)      :: 24 of 139
            Pseudo Genes (multiple problems)  :: 22 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102907.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KW4"
                     /isolation_source="well water"
                     /db_xref="taxon:1463165"
                     /plasmid="pKW4-4"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            complement(join(4843..5207,1..133))
                     /locus_tag="NWT71_RS26375"
                     /old_locus_tag="NWT71_26375"
     CDS             complement(join(4843..5207,1..133))
                     /locus_tag="NWT71_RS26375"
                     /old_locus_tag="NWT71_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<174..872)
                     /locus_tag="NWT71_RS26505"
                     /pseudo=""
     CDS             complement(<174..872)
                     /locus_tag="NWT71_RS26505"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(811..1152)
                     /locus_tag="NWT71_RS26385"
                     /old_locus_tag="NWT71_26385"
     CDS             complement(811..1152)
                     /locus_tag="NWT71_RS26385"
                     /old_locus_tag="NWT71_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1766..1897
                     /locus_tag="NWT71_RS26390"
                     /old_locus_tag="NWT71_26390"
     CDS             1766..1897
                     /locus_tag="NWT71_RS26390"
                     /old_locus_tag="NWT71_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            complement(2117..2926)
                     /locus_tag="NWT71_RS26395"
                     /old_locus_tag="NWT71_26395"
     CDS             complement(2117..2926)
                     /locus_tag="NWT71_RS26395"
                     /old_locus_tag="NWT71_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(2919..4169)
                     /locus_tag="NWT71_RS26400"
                     /old_locus_tag="NWT71_26400"
     CDS             complement(2919..4169)
                     /locus_tag="NWT71_RS26400"
                     /old_locus_tag="NWT71_26400"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4621..4836)
                     /locus_tag="NWT71_RS26405"
                     /old_locus_tag="NWT71_26405"
     CDS             complement(4621..4836)
                     /locus_tag="NWT71_RS26405"
                     /old_locus_tag="NWT71_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1237..1412)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          1359..1875
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.076"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..821)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.23e-180"
     Typing          complement(818..1131)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.04e-58"
     Typing          complement(4506..5206)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="6.01e-143"
ORIGIN
        1 cgcttgcgtt cagttccgat ttgacaacgt tctcgtgctt gctgaatgcg gctttcagcc
       61 tctcttcggt aagctgctgc tgtgctttcg atttctcctc gaagtccctc gaggccgtta
      121 aaagactgct cataaatggc tcccctcagt cggatatttc gccccccgtc cgggtcggca
      181 atgctgatac tgttttttgt ggttctgacc acctcaaaac cggccgctgt gagcgcccct
      241 gtgacgtcat cacgcgtttt cagctctcct gatgaggcta acatcagcaa accccgtgta
      301 atcgtctctg cggcttcctg cttcgtttct ggcagtcctg aaggcgtgac cagcgcccgc
      361 cggttctccg ggtcgttcgg atcatgtagc cccagcctgc cgttgaccac tgtctgccac
      421 gcatcgatgc gcggccggtc ggcacgatcg taatacggct ggaggcgttt ccccgtcagt
      481 aattcggtat tggggatcag aaaattcagc tcaagacgac ccttgtctgc gtgttccacc
      541 cacagaatgc tgtactggtc tttatcgagt ccgggcatca gaacccgctc aaagctggcc
      601 atcagttttt cacgctgacc cggcggcagg tccgcctccg cgaatgacag caccccggag
      661 gtatattttt tcacatacgg cgacgcatca atcagctccc ggacttcttc cggcttcccc
      721 tgcaggacgc tggcaccttc acgctgccgg tctttaccca gaagataatc cacaggaccg
      781 gcgcccccgc cgcgaccccg gggatggaat ttaacaatca tcgtccggcc ctttttccag
      841 tacggcatgc cgcaaccgtt ccagaccggc atcgatcgcc atcagcgctg ccacgatctg
      901 cacccggtca tgaccggcac caccggcgtt cactttccgg gcgatctggt tcaggttatt
      961 gcccatgccg gcaagctgac gcagcagcgc gggcgaaatc gagggaagcc tgccggcgcg
     1021 tgtcggtttc tcatcaaggc aggtctggcg catccacgcc gccagttgtt ttccgtcgca
     1081 ccgctcaagc aaccgccggt gttcgtcttc cgtcacccac atcgtgagca tcttgttgcg
     1141 tttatctgcc agaatcgcct cctgataacg ccgtgacgga cgggaaagcc tttcccggtc
     1201 ggcgcggggt tggacaagcc gcaggcgcgt cagtggggtt tcggggcgca gccctgaacc
     1261 agtcacgtag cgctagcgga gtgtatactg gcttagtatg ttacggcgag atggagattc
     1321 ggggaagtgc ttcatgtggc aagggggaaa atacggctgc ggggcgtaag tgcaaaatgc
     1381 aggtaacgat gaaatgacgc ctcctcgctc actcggtcgc tgcgctcctg ccgtgagact
     1441 gcggcgggcg ttaccagctc acgaaggacg agataacgag atcgcgctca agtcaggaaa
     1501 gtcaggaaaa aggatgcggc gttgccgttt ttccataggc tccgcccccc tgacaagcat
     1561 cacaaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagatcccag
     1621 gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     1681 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     1741 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc
     1801 cttgtccggt aactatcgtc ttgagtccaa cccgaaaaga catgcaaaag cgccactggc
     1861 agcagccact ggtaatcgga ttcacggagc ggagtagagt tatctacaga gttcttgaag
     1921 tggtggcctg actacggcta cactgaaagg acagttttgg tatctgcgct ccgtgaaagc
     1981 cagttacctt cggaagaaag agttggtagc tctaaattcc ggcaaacgaa ccaccgttgg
     2041 tagcggtggt ttttttgttt acggcttagg agattacggc tagaacgaaa ggatctcaag
     2101 aagatcctct cagaaattat acagacatgc ggtagtttga gatgtcagag ctatctaact
     2161 cttttaccgg aaccagtttg taatgccttt cctcaatcaa tttcacttgt ccttcgtcta
     2221 tctttagctc aaacagtgca atcaaatcgc tcgaaacaaa ctgagcggaa atagctctac
     2281 atctaacatt tgggtatttt tgttcacacc acgctgtatc ttgcgatgtt tgcaccattg
     2341 atatttgatc tgtcccacct tttgcttgaa ctggtattat ataatggcaa ccgtatttat
     2401 ctatacctac ataaagttca tcgatctcta tttgaccaat tcccttcacc gtagttctta
     2461 aatgattctg taaagaatat gtggttatac ctagaaatat atctattagc cggttatatc
     2521 tcaccttcgc cagcaatgcc tgctcgtcgt ctaaagaata acctgtaatt atctccgggg
     2581 tattgtctgg aatttttatt gccaccatat taggatttgg caaaatccta ctaatagtta
     2641 ctaatctaaa tcgatacttt gacctaccag ccagctcaat aatccactcc ttaccttctg
     2701 gctgagtcaa cagaatactt tctggcatag gcgttctaaa tctgattgaa tatataacat
     2761 caccaacatt agatgggagt tctataccta aatcttttgc agcactgttt atatctaacc
     2821 tttcaaacaa aacctctaag tcaccatcaa catatttatc gaaaaatatc ttttctacca
     2881 actgatggta tctatttttg gacctttctt ttttatcctt actcacaaac aacctccttg
     2941 ttactatttt ttattctctt agcaattaca tttgatgaca caccaaacat agatgcagca
     3001 tcggacatag aaatagcaac caaactttca tcaccgagtt tgattttctt cgttggtttt
     3061 tttggcgtaa caccaagaac cttcataatc tgcaagccaa cacttctagc tagcatagga
     3121 ggaacactat taccgacctg tctaaatcca tgccacttag tcacatgaaa tctgaaccaa
     3181 tcaggatatg aatgcagtct tgctgcctct ctgaccgtga tgaccctagg atagatcgga
     3241 tgtataggac gaggagatgt aaaagcacct ctgtcacttg ctgttcctgc ccttaaagta
     3301 ttacatagcc cattcatcga gagcttttta aacctgctga ttggttctac cgtgtcaggc
     3361 aaagtttcaa caaaccgccc tctagaaatg tcagagtgat ctgtcagtaa agaacacgtt
     3421 aatttgtctt tattaaaaat ccttgggtag ctaaaatcac taggatcttt tcctacgcca
     3481 tgcaaatagg atgagtatag tgattgctca ccaaatttag cataaaccca atctctaccc
     3541 catagctcag gatatttttc cgcatcagga atatccccaa tagcgtcccc aaccgttgtt
     3601 ctaatggata atggctcagg gtatgatggc agagcaattc ctttccttcc ccccattaag
     3661 aaaagacgac ggcgatcttg aggaactcca taatcagctg cgtttagcac tttataatcg
     3721 cttacaacgt cgtacccgcc atctttaaat gcatccatta tctctactaa aaactgctta
     3781 tgcttaccaa ccgtcaaccc ttttacattt tcaaaaacaa agtatttagg ctttagctca
     3841 agaaccagcc gtacatagtg atagaccagt ttgtttctat catcatcaag cgcgcgtttt
     3901 cctattaatg aaaagccttg gcagggagcg cctccgatca ctacatcaat ctctttctca
     3961 ccaatacctg aaacttccct aacgtattct ccggtaacat caacaacact tctacatatc
     4021 gttttgcaca taggaaagtt atatttgtga gcagagcaat gtattggatc tatttcaact
     4081 gatgccatta tatcaaagcc agcttgctcg aaaccgagac tcagcccacc cgctccagca
     4141 aataaatcaa cagcaatcgg tctagtcatt ctttacaccc acgttatata aaatcaacat
     4201 gatgctatca gaaaaacgat cgctttaccg atcgtttttc tgatttcgat tgaacataac
     4261 atctcttacc cgcaccgcgc cggaggcact cagggcgttt ctgatggctg acgggcaaaa
     4321 ggtggtggca agtacaccga aaaccaccag aaacgcacct gatttatttt acgcatatgc
     4381 atcacttatt cacatcactg catagccggt gatttcggtt aaaaaagact ctctgtgtgc
     4441 tgaaatcgca gaaaatggcc atgatccccg tcacgctcag gccacctgag acgggcgttt
     4501 atcagtctcc atactccctg tcaccatgag ataatgtgcg ttgccgttcc ggtaccagat
     4561 ctgctgtctt ctctggttcc ttttccgttg tttgcccgta tcgctgttgc tcaatctcat
     4621 tcagcctgtc gataaccggc gcagctgctc gctcaaactc tgcacctgcc tgctcaagcc
     4681 cgacacgcgc tcgctcagcg ccgcgttctc ccgttgcata agaccagaca tcttccgcca
     4741 ttcctcgaag gcgctctccc actcgtccag ccttctcgag tagtcctgct gtagctgctc
     4801 taacgcgctc agcaattgtt tttccagttc cgtcattctg ctctactccc gtaccagctt
     4861 caccgcgttc cgcgtcttgt tgtccaccgt ccggcccgtt tcggctttca tcccctccgg
     4921 cagcaccaga aaccgtccgt tgcggttctc cagataggtc acgccccacg tctgcatgtg
     4981 cagtttcgac agcgtatctc tctgctgcct gatttccgcc aggttcgccg ctatccggtt
     5041 cccctggtac cacagcaccc cgctcaggct ggcgaacagc agccctgaca ccatggtgat
     5101 ggtcagccac gtccgcccga ccattcgcag cacgctcagc cggttcgact gcatcgccgc
     5161 gttcatcccc tgttcgtggg cgctgatggc gtcgttgatt ctcttcg
//
