LOCUS       NZ_CP102906            13970 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain KW4 plasmid pKW4-3, complete
            sequence.
ACCESSION   NZ_CP102906
VERSION     NZ_CP102906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054364
            Assembly: GCF_024734275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13970)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13970)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 326.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:11:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,295
            CDSs (total)                      :: 5,172
            Genes (coding)                    :: 5,033
            CDSs (with protein)               :: 5,033
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 50 of 139
            Pseudo Genes (incomplete)         :: 89 of 139
            Pseudo Genes (internal stop)      :: 24 of 139
            Pseudo Genes (multiple problems)  :: 22 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102906.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13970
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KW4"
                     /isolation_source="well water"
                     /db_xref="taxon:1463165"
                     /plasmid="pKW4-3"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            11..643
                     /locus_tag="NWT71_RS26310"
                     /old_locus_tag="NWT71_26310"
     CDS             11..643
                     /locus_tag="NWT71_RS26310"
                     /old_locus_tag="NWT71_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901447.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_017901447.1"
                     /translation="MAWIVGFISQKGGVGKSTKARALAREASACGIKTKLADLDLEQAT
                     SAEWHRRRLSAGLPPVASVEVFSTAKQAIESAGEFDLLILDGPARASKGTSEIAKVADL
                     IVQPTGASLDDLVPAIKVFNALVKEGVPRSKLVFALSRVGTEAEELDARAYISEAGYET
                     LAGCLFEKPAYRKAMNSGLAVTETRYKGLNERADELIQALIDKIGEE"
                     /name="ParA family protein"
     gene            643..1017
                     /locus_tag="NWT71_RS26315"
                     /old_locus_tag="NWT71_26315"
     CDS             643..1017
                     /locus_tag="NWT71_RS26315"
                     /old_locus_tag="NWT71_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017901448.1"
                     /translation="MADASKLKTKRARGGLGVPPGPDEVATSLNAPEIAPAVAATDVQI
                     QQADLSPVHHDPVGTQTVTEAYTRRDGRSARKTNRTLAFATRVTPAFDQEIRDIAEREG
                     LKLVEVLEKAVEAYKEKQGY"
                     /name="hypothetical protein"
     gene            complement(1207..2327)
                     /locus_tag="NWT71_RS26320"
                     /old_locus_tag="NWT71_26320"
     CDS             complement(join(1207..2069,2069..2327))
                     /locus_tag="NWT71_RS26320"
                     /old_locus_tag="NWT71_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="WP_087799212.1"
                     /translation="MKKRFSDEQIISILREAEAGVPARELCRKHAISDATFYTWRKKYG
                     GMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCDAT
                     GLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRREGL
                     HVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALSTGRRI
                     KCLTCVDDFTKECLTVTVAFGISGVQVSRILDSIALFRGYPATIRTDQGPEFTCRALDQ
                     WAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRQDYNE
                     CRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
                     /name="IS3-like element ISSen4 family transposase"
     gene            complement(<2400..2504)
                     /locus_tag="NWT71_RS26325"
                     /old_locus_tag="NWT71_26325"
                     /pseudo=""
     CDS             complement(<2400..2504)
                     /locus_tag="NWT71_RS26325"
                     /old_locus_tag="NWT71_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /name="Rpn family recombination-promoting nuclease/putative
                     transposase"
     gene            complement(2674..3294)
                     /locus_tag="NWT71_RS26330"
                     /old_locus_tag="NWT71_26330"
     CDS             complement(2674..3294)
                     /locus_tag="NWT71_RS26330"
                     /old_locus_tag="NWT71_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152402.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_017901250.1"
                     /translation="MFVFGYLRASTSEQDASRAKNALKAFANQHGHRIAGWYIDNVSGT
                     TMNRPELIRLLGDAEPGDVILIEQVDRLTRLDDAGWETLRKQITDKQLAIVSLDLPTSH
                     LALSSTVSDDFTRSMLKAVNGMMLDMLAAIARKDYEDRRRRQSEGISKAKAEGKYRGRV
                     ADAQKHELIRTLRLVNGKSLRETARLAGVSKMTVIRVCSSANK"
                     /name="recombinase family protein"
     gene            4133..5101
                     /locus_tag="NWT71_RS26335"
                     /old_locus_tag="NWT71_26335"
     CDS             4133..5101
                     /locus_tag="NWT71_RS26335"
                     /old_locus_tag="NWT71_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_087786069.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLDD
                     GAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLR
                     CPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDTTGLKVFGEGEWKVRKHGKERRRIWRK
                     LHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTEYNRRSIAETAMYRMK
                     QLLGDSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            5759..6148
                     /gene="stbA"
                     /locus_tag="NWT71_RS26340"
                     /old_locus_tag="NWT71_26340"
     CDS             5759..6148
                     /gene="stbA"
                     /locus_tag="NWT71_RS26340"
                     /old_locus_tag="NWT71_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_003032490.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEYS
                     FSVTAKGLEMALYRTRQNRKNVLHNTHDKSSAKGAAESVLHNTQPSEPEAQESEKAESP
                     GIIDKEFFNKIGEDFNPKKFNKKFW"
                     /name="stbA"
     gene            complement(6159..6856)
                     /locus_tag="NWT71_RS26345"
                     /old_locus_tag="NWT71_26345"
     CDS             complement(join(6159..6607,6607..6856))
                     /locus_tag="NWT71_RS26345"
                     /old_locus_tag="NWT71_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_255059029.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRVQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLPGLLSAFEVVVW
                     MTDGWPLYESRLKGELDVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            7023..8189
                     /locus_tag="NWT71_RS26350"
                     /old_locus_tag="NWT71_26350"
     CDS             7023..8189
                     /locus_tag="NWT71_RS26350"
                     /old_locus_tag="NWT71_26350"
                     /inference="COORDINATES: protein motif:HMM:NF016145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="WP_047680983.1"
                     /translation="MKINLVGAVGGGNFGDEFILNCCIAEHIKNKDAKVSVSGFSPDII
                     LKSGFSVENHSLNFFNILDKLRQKAENGLKISMNDLISQFEGAEEFDAIHFIGGGYINS
                     LWPSNYALLGIAYIYSKIHKKPIYATGLGLYPYEENESLTSLFNSLDIIDVRDEKSKSL
                     IPSASFTGDDALLALNEPSMLVKNDDSPALILSLQSHLFEGQSLIEKIFTDGTFKELKN
                     KKIKKIIIIEAAPEDNIPFSRETFNNTLANGIEIEFVTGNEILRRGIPYNSRSFVISSR
                     YHINLIYSMLNVSGIAVYQNEYYRNKHKSVTEMGGAWSILNHDKLSEALDHWLPLDSEN
                     YTSPNNMINKAKEKKELFNKIIANAQNKDKKSISLESALAVINKFITK"
                     /name="polysaccharide pyruvyl transferase family protein"
     gene            complement(8203..9666)
                     /locus_tag="NWT71_RS26355"
                     /old_locus_tag="NWT71_26355"
     CDS             complement(8203..9666)
                     /locus_tag="NWT71_RS26355"
                     /old_locus_tag="NWT71_26355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_050515768.1"
                     /translation="MLRIESFEALMEEMKFSLVDESLARKDYDRLTNSYYGKSCNPLIF
                     CENLLIIGVRSGLEFLIAKSVNSNAVVHIVESNKSLLEKINKLNIANVTAFSSFKEYIS
                     SVKSQKYDYVRIDRVNFNLDLVAQLYSKHNISSICGELKEADCKPLTLYRLSRNSCDSF
                     FFNIKDKNYNVAGARKCIEQPEVSVVVAAYGVENYLDECVSSIVHQTLKNIEILIVDDG
                     SIDGTGKKADEWQKRFPDIVRVIHKENGGCASARLEGMREAKGEYVAFVDGDDWVEQPM
                     YEDLFESAALHNSEIAQCGFYEFFADQTKNYYSTAWGADGQNGTTGLVKEPTEYLTLMP
                     SIWRRIYKTSFLRKHGIEFPVQIRRHDDLPFAFMTIARAKRISVIPDCYYAYRLNRPGQ
                     DVGATDERLFIHFEIFEWLYEQVRPWASMAIMSNMRLVEIGTHSWVLSRLDSHLKNDYL
                     NKSISGISERYKRYELDDGWLDRLKLASR"
                     /name="glycosyltransferase"
     gene            complement(9670..10986)
                     /locus_tag="NWT71_RS26360"
                     /old_locus_tag="NWT71_26360"
     CDS             complement(9670..10986)
                     /locus_tag="NWT71_RS26360"
                     /old_locus_tag="NWT71_26360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.3"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_087799100.1"
                     /translation="MNIMVFICAQSFFKAEIMEWFSDIKNSKIDLSIIIPMYNVEDYII
                     ETLNSMRGVRGFKVELILVDDGSTDNTLNLAKEWANSSDFDTFILSKENGGPSIARNLG
                     LSKARGELVTFFDSDDIALSYLYTDIINIMLSHGVDFCIFRGSSFDHVTQKVHEFPDYY
                     VWEKIMGGAEFKVLTPQQEPRLARLEPSAVVRVYRKDFITSNEIQFPENLFFEDVLFHA
                     KCVLNAKKIALLNKTLLLYRVNREGQTTGSFGKKRRDVLKVIDLIISDYSKMKVTNDIW
                     ANLVGLLIRMNVWCSENCAYQDKADFVKESISLFSKLPKNVLEIYMNEYSYNDWEIKLC
                     KAFIERNEKVLNVSAEGGYPIFDNVHESLHPEENQVSLVSIDGKLEHLFSQQKEGWAHD
                     RFNVLNGKLESLIAQNKKLYEKLEHKRSISSLINKIFKG"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(<11254..11481)
                     /locus_tag="NWT71_RS26365"
                     /old_locus_tag="NWT71_26365"
                     /pseudo=""
     CDS             complement(<11254..11481)
                     /locus_tag="NWT71_RS26365"
                     /old_locus_tag="NWT71_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            12254..13009
                     /gene="repE"
                     /locus_tag="NWT71_RS26370"
                     /old_locus_tag="NWT71_26370"
     CDS             12254..13009
                     /gene="repE"
                     /locus_tag="NWT71_RS26370"
                     /old_locus_tag="NWT71_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="WP_001568031.1"
                     /translation="MAEIAVINHKKRKNSPRIVQSNELTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGSLQEHDGICEIHVAKYAETFGLTSAEASKDIRQALKGFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGVVSLKIDWIMERYQLPQSYQRMPDFRRRFLQASVDEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTE"
                     /name="repE"
     Typing          complement(5289..5545)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.26"
                     /coverage="100.0"
                     /evalue="3.17e-72"
     Typing          12114..12500
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agggggctag atggcctgga tagttggttt tatttcccag aaaggtggtg tgggcaaaag
       61 cactaaggcg agagcgttag cgcgtgaagc atccgcctgt ggcatcaaaa caaaattagc
      121 tgacctcgac ctggaacagg caacgagtgc tgagtggcac cgccgacgtc tgtcagctgg
      181 tttaccaccg gttgcttcag tcgaagtatt ttcaacagca aagcaggcca tagaatctgc
      241 cggcgagttt gatcttctca ttcttgatgg accagctcgt gcaagcaaag ggacttcgga
      301 aattgctaag gtggccgacc ttatcgtcca gccaacgggg gcttcccttg acgatcttgt
      361 cccggcaatt aaagttttca acgcgctggt aaaagaaggc gtgccacgtt caaaactggt
      421 ctttgcttta agccgtgtag gtaccgaagc ggaagagctg gatgctcgtg catatatcag
      481 tgaggcgggt tatgaaactt tagctggatg tctttttgag aagccagctt acagaaaagc
      541 gatgaactca ggacttgcag tgacagaaac acgctataaa ggtttaaacg agagagctga
      601 tgaacttatc caggctctca tcgataagat tggagaagag taatggctga tgcatcaaaa
      661 cttaaaacta aaagagcccg tggtgggctg ggtgtacccc caggccctga cgaagtggct
      721 accagcctga acgcgcctga gatagctcct gcagtcgctg ccactgatgt acagatacag
      781 caggcggact tatcgccagt gcaccatgat cctgtgggta cacaaacagt gaccgaggca
      841 tatacccgcc gcgatggccg ttctgccaga aaaacaaaca ggacgctggc atttgcaaca
      901 cgtgtcacac ctgctttcga ccaggagatc agggatatcg cagagcgtga ggggttgaaa
      961 ctggtggaag ttctggaaaa agcagttgag gcatataagg aaaaacaggg atactaatcc
     1021 ggaatgcagc aatcttgctg cattcttttt accgttgaaa tcttgcagca agatttatgc
     1081 tagctaaatg ctagattgct gcaagataaa tgatataata ctcaaactga gtaattaaac
     1141 gacccggtga agtacgcagt ttgagtgctt acctgcgtga cctgccccca cgattagata
     1201 caacgctcag ttagtaacgt cggaatcttc attctcagaa tgaccctttc tccagcccgc
     1261 tgcaaattca gacggtgtct gataattcag cgtggagtgc gggcggcatt cgttataatc
     1321 ctgccgccag tcattaataa ttttcctggc atgaacgata tcgctgaacc agtgctcatt
     1381 caaacattca tcgcgaaatc gtccgttaaa gctctcaata aatccgttct gcgttggctt
     1441 gcccggctgg attaagcgca actcaacacc atgctcaaag gcccattgat ccagtgcacg
     1501 gcaagtgaac tccggcccct ggtcagttct tatcgtcgcc ggatagcctc gaaacagtgc
     1561 aatgctgtcc agaatacgcg agacctgaac gcctgaaatc ccaaaggcaa cagtgaccgt
     1621 caggcattcc tttgtgaaat catcgacgca ggtaagacac ttgatcctgc gaccggtgga
     1681 aagtgcgtcc atgacgaaat ccatcgacca ggtcagattg ggcgccgccg gacggagcag
     1741 cggcagacgt tctgttgcca gccctttacg acgtcttctg cgttttacgc ccaggccact
     1801 gaggtgataa agccggtaca cgcgcttatg attaacatga agcccttcac ggcgcagcaa
     1861 ctgccaaata cgacggtagc caaaacgcct gcgctccagt gccagctcag tgatgcgccc
     1921 tgataaatgc gcatcagcag ccggacggtg agcctcatag cggcaggtcg acagggataa
     1981 acctgtaagc ctgcaggcac gacgttgcga cagaccggtc gcatcacaca tcaacatcac
     2041 ggcttcccgc ttctggtctg tcgtcagtac tttcgcccaa gagccacctg aagcgcctct
     2101 ttatccagca ttgcttcggc aagcagcttc ttgagtctgg cgttctcttc ctcaagcgac
     2161 ttcaggcgct taacttcagg cacctccata ccgccatact tcttacgcca ggtgtaaaac
     2221 gtggcatcgg aaatggcatg cttgcggcag agttcacggg cgggtacccc agcttcggct
     2281 tcgcggagaa tactgatgat ctgttcgtcg gaaaaacgct tcttcatggg gatgtcctca
     2341 tgtggcttat gaagacatta ctaacatcgg ggtgtactaa tcaacgggga gcaggtcaca
     2401 gcgggcaaat acaacttcat aaaatcccgg gcaatatccg gctgcgtcag gaactgcctg
     2461 aacgtggcat cgtggggtgt gggggtgctg tttttcttca tcatcagccc tggctctgaa
     2521 aaatgacccg ggtatgctat cacaggcagg agcagcacat aatggccagt cagatgatga
     2581 tgtatttctt tcccgtaagg ccgctcagtc tatattcttg catatcaatc attcaggatg
     2641 ccggacaact cacgtttgcg aacagagaga tcgttatttg ttagcgctgg agcagacccg
     2701 aattactgtc attttagaaa cgcccgccag gcgagctgtt tctcgcagcg attttccatt
     2761 gaccaggcgc agagtgcgta tcagttcatg tttctgcgca tcggccaccc tgccccggta
     2821 ttttccttca gctttcgcct tactgatacc ctctgactgt cgacgtcgac ggtcttcgta
     2881 atcttttcgg gcaatagcgg ccagcatatc cagcatcatg ccatttacgg ccttcagcat
     2941 gcttcgggta aagtcatcac ttactgtgct ggatagcgcc agatggctgg tcggtagatc
     3001 aagactgacg atggccagct gcttgtctgt aatttgcttt ctgagtgtct cccagcctgc
     3061 atcatccagg cgagtgagcc tgtcgacctg ttcaatgaga atcacatcac caggttccgc
     3121 atcaccaaga agcctgatca gttcaggacg attcattgtc gtaccggaga cattatcgat
     3181 ataccagcca gcaatacggt gtccatgttg gtttgcaaac gccttcagtg cattttttgc
     3241 ccggctggca tcctgctctg aagtggatgc ccggaggtaa ccaaaaacga acattttttg
     3301 tcctagtggt aacatttaag tggtactgtt catcatggta tcacttaagt ggtaacaata
     3361 aaactacccg atacctcttg aggtggtaca tatgaactat accctaatgt gacatattgg
     3421 cgagtggtct aggattggtt aataaaaaag ccagggaatc ctggctcttg ggtgagctgg
     3481 ttgttagaag aaccagcgaa ttaactctaa aagcacaatg agcgctttca gagcctggat
     3541 gacttttgta agagtctcca ttttctctcc ttttctggag agtagaacac cgaatgaacc
     3601 cttccactct gtcattcggt ttttaatctt taatgtcagt taaagatagt tccactcagg
     3661 taatggtact gactcagcac cacctgttag tcagccagag tctaatcaat gtgttagctg
     3721 gctcttgtaa atcagattga tcagagtaaa gagtgggggg tatcattcaa ccgtcagtta
     3781 aagatagttc cattccagat ctgagtacca gcttagtttt ggatgtaaaa aagcccgatc
     3841 accagtcggg cttttttaca tctaaacccg cttgaagcct tatcatccgc tgccttgacc
     3901 ccaattatgg gttaatttat tactgtaacg cattcctaat aatcgcgatt ttattgatct
     3961 agaacaaaga aaaaatgtca gagtacaagt gaagggcgct tgcgctaaat gtaaatttat
     4021 acgattaaaa aaagatcatt tttactacta acaattattc ttgttcttag tcattcatct
     4081 gaaaggtaat gcttccaaat ggctttgttg aataaatcga acttttgctg agttgaagga
     4141 tcagatcacg catcctcccg acaacacaga ccattccgtg gcaaagcaaa agttcagaat
     4201 caccaactgg tccacctaca acaaagccct catcaaccgt ggctccctca ctttctggct
     4261 ggatgatggg gcgattcagg cctggtatga gtcggcaacg ccttcatcac gaggaaggcc
     4321 ccagcgctat tctgatctcg ccatcaccac cgttctggtg attaaacgcg tattccggct
     4381 gaccctgcgg gctgcgcagg gttttattga ttccattttt gccctgatga acgttccgtt
     4441 gcgctgcccg gattacacca gtgtcagtaa gcgggcaaag tcggttaatg tcagtttcaa
     4501 aacgtccacc cggggtgaaa tcgcacacct ggtgattgat accaccgggc tgaaggtctt
     4561 tggtgaaggc gaatggaaag tcagaaagca cggcaaagag cgccgtcgta tctggcgaaa
     4621 gttgcatctt gctgttgaca gcaacacaca tgaagttgtc tgtgcagacc tgtcgctgaa
     4681 taacgtcacg gactcagaag ccttcccggg ccttatccgg cagactcaca gaaaaatcag
     4741 ggcagccgcg gcagacgggg cttacgacac ccggctctgt cacgatgaac tgcggcgtaa
     4801 gaaaatcagc gcgcttatcc cgccccgaaa aggtgcaggt tactggcccg gtgaatatgc
     4861 agaccgtaac cgtgcagtgg ctaatcagcg aatgaccggg agtaatgcgc ggtggaaatg
     4921 gacaacggaa tataaccgtc gctcgatagc ggaaacggca atgtacagaa tgaagcagtt
     4981 gttgggagat tcactgacgc tgcgtgacta cgatggtcag gtagcggaag ctatggccct
     5041 ggtacgagcg ctgaacaaaa tgacgaaagc aggtatgcct gaaagcgtgc gtattgcctg
     5101 aaaatccagc cagctacagg gtcgttcgca cgaaatctga tttattcaac aaagccgatt
     5161 accacctgaa accaggtcta tataggccaa aagttcatct gatacttttg cggttattgt
     5221 tggcattcag tcctcacttt gtgcattttt taaacgaaaa aagggtgtct aatacggtga
     5281 aatcatagta ttaccaaagt aataaataaa aaattcttta aatcagtaag ttagattgga
     5341 tttgtaatac ctgggtaata ccgcagtatg gctaaatttg gttttttttc ttttaaatca
     5401 gtggtatagc gtgatttatc acgctgcgtt aggtgtatag caggttaagg gataaaaaat
     5461 catctttttt ggtagggcga tctccgtagg ttaagggtca tttggctaaa aagcgtccta
     5521 ttctttgatg gtcatgcttg catgaccatc tgagcaacca aaaactacag ataaactaca
     5581 aaaaacgttt tgccttagtg ttggcagact acaaatagac tacaagaaaa ctacaaataa
     5641 actacaaaaa acgtgacaga ctacaaatag actacaagaa aactacagat aaactacaaa
     5701 actcgattga ccccttctta cgagtgttgt agagtcattt tcatacaacg ggggggttat
     5761 gaataaacca gcagatctga aaccccgcaa cttatcggct gctgtcagat tgcgcctaaa
     5821 tgaaatcgag aactggctgg acagagggct aacgcggcat gaaattgctg aaatcctcga
     5881 cagcgaatac agcttttcgg taacagccaa agggcttgag atggcactgt atagaacgcg
     5941 gcaaaaccga aaaaatgtat tacacaatac acatgataag agtagcgcga agggtgcagc
     6001 ggaaagtgta ttgcacaata cacaaccgtc tgagcctgaa gcgcaggaaa gtgaaaaagc
     6061 agagagtccc ggcattattg ataaagaatt cttcaataaa atcggtgagg atttcaaccc
     6121 taagaagttc aacaaaaaat tctggtaatg actccaactt actgatagtg ttttatgttc
     6181 agataatgcc cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagtgacttc
     6241 ctgcccagcc ttgccagatg ctgcctcaga ttcaggttat gccgctcaat gcgctgcgta
     6301 tatcgcttgc tgataacgtc cagttctccc ttcaggcgtg attcataaag tggccagcca
     6361 tccgtcatcc ataccacgac ctcaaaggcc gacagcaggc ccggaagacg ctccagcgtg
     6421 gccagcgtgc gttcaccgaa tacgtgcgcc acaaccgtcc tccgtatcct gtcatacgcg
     6481 taaaacagcc aacgctgacg tgatttagca ccgacgtagc cccactgttc gtccatttca
     6541 gcgcagacaa tgacgtcact gcccggctgt acgcgtgagt ttaccgactg tggcctgagt
     6601 tttttaagtg acgtaaaatc gtgttgaggc caacgcccat aatgcgtgca ctggcgcgac
     6661 atccgacgcc attcatggcc atatcaatga ttttctggtg cttaccgggc tgagaggcgg
     6721 tgtaagtgaa ctgtagctgc catgttttac ggcagtgaga gcagagatag cgctgatgtc
     6781 cggcagtact tttaccgtta cgcaccacgc cttcagtagc tgagcaggag ggacaactga
     6841 tggagatgga agccacggga gcacctcaaa accaccatca tacactaaat cagtaagttg
     6901 gcaccattac catgaaatca taagcttcaa tgactataat ttggaataaa aaattaagat
     6961 acatctgcat tggatctctt tatttaattg tgtaggataa aattaaaaca aaaggaaaaa
     7021 tcatgaagat aaatttggtt ggtgcggttg gtggcggcaa ttttggagac gaattcatat
     7081 tgaattgctg tatagctgag catatcaaga acaaagacgc aaaggtttca gtcagtggat
     7141 ttagccctga cattatttta aagagtggct tttcagttga aaatcattct ttaaattttt
     7201 tcaacatact agataaatta agacaaaagg cagaaaatgg attaaaaata tccatgaatg
     7261 accttatctc acagtttgaa ggcgccgagg aatttgatgc cattcatttt ataggcggtg
     7321 gctatattaa ttcactttgg cctagcaact atgccttact aggcatagct tacatttatt
     7381 caaaaatcca taaaaaacct atttatgcca caggccttgg actataccca tacgaagaaa
     7441 atgaaagttt aactagcctt tttaatagcc tggacataat agatgtaaga gacgaaaaat
     7501 caaaaagttt aattccatca gcttctttta caggagatga cgccctcttg gccttaaacg
     7561 aacccagcat gctggttaaa aatgacgact ctcctgcatt aatattatca ttacaaagtc
     7621 atctatttga aggacagtct ttgatagaaa aaatcttcac cgatggaaca ttcaaagaac
     7681 taaaaaacaa aaaaataaaa aaaataatta taatcgaagc agcaccagaa gataacatcc
     7741 ccttttcaag ggaaacattt aacaacacct tagcaaatgg aatagaaata gaattcgtga
     7801 caggaaatga aattttaaga cgcggcatac catataactc aaggagcttt gtcatatctt
     7861 ctagatatca tatcaactta atttattcaa tgctcaacgt tagtggaata gctgtatacc
     7921 aaaatgaata ttatagaaac aagcataaat cagtaacaga aatgggaggg gcgtggtcaa
     7981 tattaaacca tgataaactc agtgaagccc ttgatcactg gctcccccta gattcagaaa
     8041 attacacatc gcccaacaac atgataaata aggcgaagga gaagaaagag ttattcaata
     8101 aaataattgc taatgcacaa aataaagata aaaaaagcat ctctttagaa agtgcattag
     8161 cagtaatcaa taaatttatt accaaataac cactatgaaa ccttagcggc tggccagctt
     8221 aagtctatct agccagccat catctaactc atacctctta tatctttcac taattccaga
     8281 aatagattta tttaaataat cattttttaa atgactatct aatctgctca ggacccagct
     8341 atgcgttccg atttcgacaa gtctcatatt actcatgatt gccattgagg cccatggtct
     8401 aacttgctca taaagccact caaaaatttc aaaatgtata aaaagccgtt catctgtagc
     8461 accaacatct tgtccagggc gattaagcct ataagcataa tagcaatcag gtatcacact
     8521 tattcttttt gcacgggcaa tagtcataaa tgcaaatggc aaatcatcat gtcttcttat
     8581 ctgcacagga aattcaatgc catgctttct caaaaacgat gtcttataaa tccttctcca
     8641 gatagacggc atcaaagtca aatattctgt tggttctttt actaatccag tagtcccatt
     8701 ttgaccatca gccccccaag ctgttgaata gtaattctta gtttggtcag caaaaaactc
     8761 ataaaaacca cattgagcta tttcagaatt atgcaacgca gctgattcga ataaatcctc
     8821 atacattggt tgctcaaccc agtcatcacc atcaacgaat gcgacatact cgcctttagc
     8881 ttccctcatg ccttctaagc gcgcagaagc gcatccacca ttttctttat gaattaccct
     8941 cactatatca ggaaatctct tttgccattc gtctgctttt tttccggtgc catctatgga
     9001 gccatcatca acgatgagaa tttcaatatt cttaagagtt tgatgtacta ttgaagagac
     9061 acattcatct aaataatttt caaccccata cgcagcaacc actacagata cttcgggctg
     9121 ttcgatacac ttacgtgctc ctgcgacatt gtaattctta tccttgatat taaagaaaaa
     9181 agaatcacaa ctattcctac tcaacctgta aagagttaac ggcttacaat cagcctcttt
     9241 caattcccca caaattgatg aaatattgtg cttagaatat aactgagcta ccaaatccaa
     9301 attaaaatta actcgatcaa ttctaacgta atcatatttt tgagacttta ctgaacttat
     9361 atattcttta aatgaagaaa aagcagtaac attggcaata ttaagtttat ttattttttc
     9421 caaaagagat ttgttacttt caacaatatg aacaactgca ttgctattaa cgcttttagc
     9481 aatcaaaaac tccaagccgg atcttactcc aatgatcagt aggttctcgc aaaatattaa
     9541 tggattgcaa gacttcccat aatagctatt agttaatcta tcataatctt ttcttgccaa
     9601 tgactcatcc actaatgaaa acttcatctc ttccattaga gcttcgaaag actcaattct
     9661 taacatatct tatcctttaa aaattttatt aatgagagat gaaattgaac gcttatgctc
     9721 taacttttcg tataattttt tattctgagc aatcaagctt tctaatttac cattcaaaac
     9781 attaaatcgg tcgtgtgccc acccctcctt ttgctgagag aaaagatgtt ctagcttccc
     9841 atcaatagaa actaaagaaa cttgattttc ttctgggtgt aatgattcat gaacattatc
     9901 aaagatggga taaccacctt cagcgctaac attcaaaacc ttttcatttc tttctatgaa
     9961 ggctttacac aactttattt cccaatcgtt atatgaatat tcattcatgt aaatctcaag
    10021 cacattcttt gggagcttgg aaaacaaaga gattgattcc tttacaaaat cagccttatc
    10081 ctgataagca caattttcac tgcaccaaac attcatcctt attaaaagcc caacgagatt
    10141 agcccaaata tcattggtca ctttcatttt agaataatcg gatataatga gatcaataac
    10201 tttgagtaca tcccttcttt tttttccgaa agaaccggtc gtctgcccct cacggtttac
    10261 tcgatagagc agaagcgttt tattcaaaag agcgattttt tttgcgttca atacacattt
    10321 agcgtgaaac agaacatcct caaaaaagag gttttcagga aattgtattt cgttggatgt
    10381 aataaaatct tttctgtaaa ccctaacgac agcgctaggc tcaagtcgtg ctaaacgcgg
    10441 ttcttgttgt ggagtaagga ctttaaattc cgcccctccc ataatttttt cccatacata
    10501 gtaatcaggg aactcatgaa ctttttgagt gacatggtcg aaagagctgc ctctgaagat
    10561 gcaaaaatcc actccatgag atagcataat attgattata tctgtgtata gatatgatag
    10621 tgcaatatca tcgctgtcaa aaaacgtcac caactcaccg cgcgccttac ttaaacctaa
    10681 attccttgct attgatggcc ccccattttc tttacttaaa atgaaagtat caaaatcact
    10741 actattagcc cattctttag cgaggttaag cgtattatca gtgcttccat cgtcaacgag
    10801 tattaactca actttgaaac ccctgacacc tctcatggaa ttcaaagttt caataatata
    10861 atcctcaaca ttatacatcg gtataataat tgatagatca atcttcgaat tttttatatc
    10921 agaaaaccat tccattatct ctgccttaaa aaaagattga gcacaaataa aaaccataat
    10981 attcatatgg tttttcgata aaagttaaac aacagtaaga ttttagcaaa gctttgacaa
    11041 ggatttcgaa cgttttttgt caaaaaaacg ctcccccccc aaaattttat ttatgaaaac
    11101 ttcaaaactg ccttagagca cttagcgcgc gctcgtaccc ccgccacgcc tgcccgcttt
    11161 gtgtagtggt tttcatgcag ggttggtgaa cttctgccgt ggaacgtggc tatacctgcc
    11221 tgaccagtgt caatacggcg tccggttaac gcttaccaga acggtggtga tggcgagatc
    11281 agaatagcgc agaggtcttc cccgtgatga aggcgttgcc gactcatacc aggcctgaat
    11341 agcttcatcc tccagccaga aagttatgga gccacggttg atgagggctt tattgtaggt
    11401 gggccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt tgtcgggaag
    11461 atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa gccctgacgg
    11521 tactgagatg acgcttacgt cagctcttac gctcagcaca agaagagttt tccatcctct
    11581 gtgggaacag gtcagacgct taccattatc gtaaaactcc gcattattaa tttcaggtgt
    11641 gtgactcagg ggccttagtc ccccttttac aggaagtagt gttctgtcac gacgggagcc
    11701 agggttaata aaaacgattc gagtatgtct gcagggacga tagaaatctt cgagggggtt
    11761 gtctcgcagt agtgggtacc tgagagcgtc tcgggggatt agtatcttcc ggccacggat
    11821 gtgttcgagc cagcagtgct cgccgcagcc gagcgacagg gcgaagcccc gagtgagcga
    11881 ggaagcacca gggaagaata ctttgtatat tctgctgacg ctcaatgcct gaaaaatcat
    11941 ctcacctagg ggttatccac ttatccacgg ggatattttt ataagatctt ttttaatagt
    12001 tgttagatct ttatttttag aatgcccaca ggcctttatc catgcgggtt ccagagaggg
    12061 agctgtgaca aaccgccctt tcagtgtgac aaatcaccct caaatagccg tccttactgt
    12121 gacaagttgc ccttaactct gtgacaaatt gccctcagga agcattgctt ttcacaaagt
    12181 tatccctccc tgttgactct gtattataaa gtgtgacaat cttaaaatct gtcacacttc
    12241 acatggacct gtcatggcgg aaatagcggt tataaaccat aaaaaacgta aaaatagccc
    12301 gcggattgtc cagtcaaatg agctgactga ggcggcatat agtctctcca gggatcaaaa
    12361 gcgtatgctg tatctgttcg ttgaccagat cagaaaatcc gacggctccc tgcaggaaca
    12421 tgacggtatc tgcgaaattc acgttgctaa atacgctgaa acattcggac tgacctccgc
    12481 tgaagccagt aaggatatac gacaggcttt aaaaggtttt gcgggtaagg aagtggtttt
    12541 ctatcgccct gaagaggatg ccggcgatga aaaagggtat gaatcctttc cctggtttat
    12601 caaacgtgcg cacagtccat ccagagggct ttatagcgta catatcaacc catatctcat
    12661 tcccttcttc atcgggctac agaaccggtt tacgcagttc cggctcagtg aaacaaaaga
    12721 gattaccaat ccgtatgcca tgcgtttata cgaatccctg tgtcagtacc gtaaacctga
    12781 tggctctggt gtcgtgtccc tgaaaatcga ctggatcatg gagcgctacc agctacctca
    12841 aagttatcag cgtatgccgg attttcggcg ccgtttcctg caggcaagtg ttgatgagat
    12901 caatagccgg acacctatgc gcctctctta catcgagaaa aagaaaggcc gccagacgac
    12961 tcatattgta ttttccttcc gtgatatcac ctcgatgaca acagaatagc ctgagtgtta
    13021 tctgtcacag atttgagggt ggttcgtcac atttgatctg gaaggttgag ggggatttgt
    13081 cacagttttg ctgttccctc ccttgcatcc tgcgcttact catagttttt ttggctgtaa
    13141 ccggtagtgg agccagaatt gagggcaatt tgtcacccta tcctgagggt agtttgtcac
    13201 agttaaatga gtgcagtttg tcacactaga ttttcccctc ttccagttag tcatatgaca
    13261 tgagggcaag ttcgacataa ttaatgatgg cagattgtca catttgttgc cctgaacacg
    13321 ctatctgtgt gtgcctatac atgtggtgtt tcacgaagcc ctggccgtcc acgaagttga
    13381 cctgtttccc acagtccggg ccgtccgcga agttgacctg ttccccacag aggatggaaa
    13441 actcttcttg tgctgagcgt aagaactatc tgccggaacg gttcctcttc gcttcctcgc
    13501 cagttcgctc gctacgctcg gttacatggc tgcggcgagc gctgcctgat tctaatagca
    13561 atctttcagc atgctagcag caataaaata gattgctgct agcatgcttc atgcagccgt
    13621 cggggcttat aatgacgtag tttccagaca gtgattttct agcgctcggc tcatgttgtg
    13681 atgtctgcgt gattgctgct ttcttgccga tttatcgata caatcctcag tgagctctca
    13741 ttgattattg ctgcaagatt gcataaagct tgctgctttc gttatcgtta acttgtcaca
    13801 agtattcggc tgtcaattcc tccagtcttc tgaatagcaa gatttaatct tgctgctaga
    13861 tttttgatta ttgcaatctt gctgcaagct gttatcttgt tcaggaagag ttactttcct
    13921 aacctcaatc aggtttatac taatgtttgc tgcaagatag cagcaagtaa
//
