LOCUS       NZ_CP102902             4353 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain KW1 plasmid pKW1-4, complete
            sequence.
ACCESSION   NZ_CP102902
VERSION     NZ_CP102902.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054361
            Assembly: GCF_024734255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 435.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 04:58:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,284
            CDSs (total)                      :: 5,160
            Genes (coding)                    :: 5,023
            CDSs (with protein)               :: 5,023
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 50 of 137
            Pseudo Genes (incomplete)         :: 87 of 137
            Pseudo Genes (internal stop)      :: 24 of 137
            Pseudo Genes (multiple problems)  :: 22 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102902.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KW1"
                     /isolation_source="well water"
                     /db_xref="taxon:1463165"
                     /plasmid="pKW1-4"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            complement(join(4080..4353,1..269))
                     /locus_tag="NWT83_RS26355"
                     /old_locus_tag="NWT83_26355"
     CDS             complement(join(4080..4353,1..269))
                     /locus_tag="NWT83_RS26355"
                     /old_locus_tag="NWT83_26355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_135712390.1"
                     /translation="MFSAILNTSKNSNAEKYKFLSLRIWRLVTVKTENSTTPKGFIDGV
                     LTRNAFDLRPNEKSVSFSRLETEESIPNDSLKNKKLMACKIRTANKMQNIFISEESANN
                     EDLRLDPETNELLKHISFSETENSKFSNDGITGFHWDLFFISKNGNKLRSGDSELSEKH
                     ILDAKDLLIEMSLLPNS"
                     /name="hypothetical protein"
     gene            complement(282..995)
                     /locus_tag="NWT83_RS26360"
                     /old_locus_tag="NWT83_26360"
     CDS             complement(282..995)
                     /locus_tag="NWT83_RS26360"
                     /old_locus_tag="NWT83_26360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040242822.1"
                     /translation="MDVQPTYYTQDAEMHEIVINIRKPSTEKRGEGKSSIFYTKTSSVS
                     KTIETKSYTTHLKPFIEKKDNEEHLWLKNLEQKAKLAAELISKGESNTDIIGVITSSLN
                     QNKTLVEDLKNAIEAHKESMKNVSDKNNESEIKGPNISLLSLFNALKFYPVISTLPKEM
                     VSQYIDADAHIVGMVIGDNNNKININFMSTGELLFRHIDKTTGRVRISGSAFFGKDENI
                     KDMKKIKKLISFVKI"
                     /name="hypothetical protein"
     gene            complement(998..1273)
                     /locus_tag="NWT83_RS26365"
                     /old_locus_tag="NWT83_26365"
     CDS             complement(998..1273)
                     /locus_tag="NWT83_RS26365"
                     /old_locus_tag="NWT83_26365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040242819.1"
                     /translation="MSKGGALAKRAKTQNDSHGGRDLIIRKMEPSFPFAIRSIQADGII
                     ILDFIGDEISPNKREAFASVLLTKTIAQDLKSKIDGYLAHQENNEE"
                     /name="hypothetical protein"
     gene            2159..2350
                     /locus_tag="NWT83_RS26370"
                     /old_locus_tag="NWT83_26370"
     CDS             2159..2350
                     /locus_tag="NWT83_RS26370"
                     /old_locus_tag="NWT83_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040242829.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDDN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2350..2751
                     /locus_tag="NWT83_RS26375"
                     /old_locus_tag="NWT83_26375"
     CDS             2350..2751
                     /locus_tag="NWT83_RS26375"
                     /old_locus_tag="NWT83_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040242827.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGARNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3594..3803)
                     /locus_tag="NWT83_RS26380"
                     /old_locus_tag="NWT83_26380"
     CDS             complement(3594..3803)
                     /locus_tag="NWT83_RS26380"
                     /old_locus_tag="NWT83_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_040242824.1"
                     /translation="MKSIKNFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     Typing          complement(1632..2038)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.311"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttgctgttc taatcttgca tgccatgagt tttttgtttt ttaacgagtc atttggtatg
       61 ctctcttcag tctcaaggcg tgaaaaagat actgattttt catttgggcg gaggtcaaat
      121 gcatttctgg ttaacacgcc atcgataaat ccttttggag tggtgctatt ttctgttttt
      181 acggtaacta aacgccatat tctgagagaa aggaatttgt atttctctgc attgctgttt
      241 ttgctagtat tcaaaatggc agaaaacatt agttctatct cttatatttt tacgaaggaa
      301 atgagttttt ttattttttt catgtctttt atgttttcat cttttccaaa aaaagcactc
      361 ccgcttatgc gtactctacc tgttgttttg tcaatgtgcc taaataaaag ctctcctgta
      421 gacataaagt ttatatttat cttgttatta ttgtctccaa taaccatccc aacaatgtgc
      481 gcatctgcat caatgtattg agataccatt tcttttggca atgttgatat tacaggatag
      541 aatttgagtg cgttgaatag agatagtaaa cttatattag ggccttttat ttcactttca
      601 ttatttttgt cactaacatt tttcatcgac tctttatgag cttcaatggc gttctttaag
      661 tcttcaacga gtgttttatt ttgatttaaa cttgaagtaa ttactcctat gatatctgtg
      721 ttgctttctc ctttggatat taattctgcg gctaattttg ctttttgttc gagattttta
      781 agccataagt gttcttcatt atcttttttt tcaatgaatg gtttaagatg agtagtgtag
      841 gatttagttt caatggtctt agatacagat gatgtctttg tataaaaaat agaactttta
      901 ccttcacctc ttttttcagt gctaggcttt ctgatattga tcacgatttc gtgcatctct
      961 gcatcttgag tgtaatatgt tggttgcacg tccatattta ctcctcgtta ttttcttgat
     1021 gagccaaata cccatctatt ttagacttta agtcttgagc aatggttttt gttaataaaa
     1081 cggacgcaaa ggcctctctt ttatttgggg atatttcatc accaataaag tctaatatga
     1141 tgataccatc agcttggatg cttcgaatag caaatggaaa agatggctcc atttttctta
     1201 ttattaaatc tcgcccgcca tggctatcgt tttgtgtttt tgcacgttta gccagtgccc
     1261 cacctttgct cattttttgt cctttttttg agttgtcatc atactgataa acatatctag
     1321 tgatgttata ccttatctat cataacttac atgtagattt atagtacctc agcttgaaag
     1381 gtgctccttt ctggtatgaa aggttaatgt tatatgtgta aagtaaagga tcttcttgag
     1441 atccttccct tctcatcgta atcttttatg cagaaaacga aaaaaccacc cggcaaggtg
     1501 gtttttcgaa ggttcagtaa ccggcaagtt tctgaaccaa ggtaactggc ttggaggagc
     1561 acagtcacca aaacttgttc tttcagttta gccttaactg gcgcataact tcaagactaa
     1621 ctcctctaaa tcagttacca gtggctgctg ccagtggcgc ttttgcatgt cttaccgggt
     1681 tggactcaag acgatagtta ccggataagg cgcagcagtc ggactgaacg gggggttcgt
     1741 gcatacagtc cagcttggag cgaactgcct tcccggaact gagtgtcagg cgtggaatga
     1801 gataaacgcg gccataacag cggaatgaca ccggtaaacc gaatggcagg aacaggagag
     1861 cgcacgaggg agccatcagg gggaaacgcc tgatatcttt atagtcctgt cgggtttcgc
     1921 caccactgat ttgagcgtca gctttgtgat gcttgtcagg ggggcggagc ctatggaaaa
     1981 acggccgctt agcggccttt ccttttcccc gtcacgcgac cgctcggttt tctcgagctt
     2041 ttatacgcat gctcctgccg ctcgccgcag ccgaacggca ggagcatagc gactgagtga
     2101 gcgaggaagc ggaaaagaga acatccctac actgagcact gacgctctga ggcctcttat
     2161 gacgaaacaa cagaaaacag cgctgaacat ggctaaattc atccagaatc agtctctgct
     2221 cctgctggag aagcttaacg agcttgatct tgatgtcgag gccgaccttt gtgagaaact
     2281 ccacgatgat gcggagcatc ttttccgtac actgtcctcg agactggatg cattacagga
     2341 tgataactga tggccagagt gtctattagt gaggctgccc gactgacggg taagagtaga
     2401 acaacactgc acaggttaat taaagcaggt gacctgtcca cctgtagcgg tgcacgtaat
     2461 gcaaaaatgg tagatacttc agagctttta cgtgtctttg gtccctttga gcaaccgaag
     2521 ggtgaacacc attctggaca ggtctctgaa cagcgtgata cagcctctgc tgaccagagt
     2581 gaacaggtga ttcgtcatct gaaacaggag attgaacacc tgaacacctt acttttgtcc
     2641 aaggattcgc acattgacag cctgaaacag gccatgctcc ttcttgaaaa tcaccaggca
     2701 aaattaacgg aatccccggc gccatggtgg aaattctgga aaaaatcctg accccgtcat
     2761 gcttcagttt tcctctccgg ccatcccggg gagttcctga accttcccaa aaatcgtcag
     2821 atctctgaac gctcctcacg actaaaaata cccacacact acgcaaaatt aaaattttgc
     2881 agatttgtat ttaatatcag tagcttactt tcctgaaatt tcggttttta aattttattt
     2941 acatagatcc gactctatga cgagtcgtca tagagtcccc ggcgagtcgt catagagtcg
     3001 gtaacgactc agggctataa aaacaatgag ttaataacga cccgactcta gacccgactc
     3061 cccgtttggt tgttttttag actcggcgac tctaggtacg actctagtga agagtcgccg
     3121 gtgaatcgtg tggtgagtcg tccggcgact cgccataacg tgattgccgg ccgtcgacga
     3181 acacgattcc tgaacgtgct gaaaaccggt tttccggctg cgccggcaac gcttcacggc
     3241 cacgcaataa attgcgtccc cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga
     3301 cttaccctgg ctgaatggcg tgaattttcg cgctatgcac ttgcgtgcat actcatgcat
     3361 gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt
     3421 accggtttcc cgtcagaaac gccctgaggc cgttttagcg gtgcgtacaa tcatgccttt
     3481 atgttaaatg acagatgcaa aaaagccccc gaaggggcct ggaggttttt ctgctaaggg
     3541 acgaaacggg ccgcctcccg gcgacctgtt cggcagctcc ctctgcgacc atcttatttg
     3601 gtcagttcag ccgtcatgtg gacacggtta ttgaactgag cgctggtgat tttgtatgac
     3661 gcaccttgtt cagcggcctg tgcggcaatt tttgcttcag cacgatccag ggtggaggcg
     3721 gtagcgctga cgctctgagc aaaggcaccg aaagacatca tggatagagc ggcaactgca
     3781 acgaaatttt tgatggattt catggtcgta ttcattaagt tattttgttc tgtatttggc
     3841 gttgttgcca tgatgtgata aatgatagga cgaccatgat ctgattaaaa tcgaaaaaag
     3901 ttgcggatgt tattcaaaaa aacagaataa aaattaagcg cgtcaaaaca agtgtgagcc
     3961 tctggtttca gcgaacagaa tgaagagcat cgagagttcg tacaattaga ggaggatgtt
     4021 aaattacctt cttgtattta ttcctaatgt tggctattcc tgcgactaag tggaggttat
     4081 tacgaatttg gtagtaatga catttcaatc aacaaatcct ttgcatctaa tatgtgtttt
     4141 tcagacaact cgctatcccc agagcgcaac ttattgccat ttttagatat aaaaaacaag
     4201 tcccaatgaa aaccagttat accatcattc gaaaacttac tattttctgt ttctgaaaaa
     4261 gagatgtgtt taagcagttc gtttgtttct ggatctaatc tcaaatcttc attgtttgca
     4321 gattcttcac ttatgaagat attttgcatt tta
//
