LOCUS       NZ_CP102901             5207 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain KW1 plasmid pKW1-3, complete
            sequence.
ACCESSION   NZ_CP102901
VERSION     NZ_CP102901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054361
            Assembly: GCF_024734255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 435.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 04:58:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,284
            CDSs (total)                      :: 5,160
            Genes (coding)                    :: 5,023
            CDSs (with protein)               :: 5,023
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 50 of 137
            Pseudo Genes (incomplete)         :: 87 of 137
            Pseudo Genes (internal stop)      :: 24 of 137
            Pseudo Genes (multiple problems)  :: 22 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KW1"
                     /isolation_source="well water"
                     /db_xref="taxon:1463165"
                     /plasmid="pKW1-3"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            complement(join(4473..5207,1..75))
                     /locus_tag="NWT83_RS26320"
                     /old_locus_tag="NWT83_26320"
     CDS             complement(join(4473..5207,1..75))
                     /locus_tag="NWT83_RS26320"
                     /old_locus_tag="NWT83_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(68..1318)
                     /locus_tag="NWT83_RS26325"
                     /old_locus_tag="NWT83_26325"
     CDS             complement(68..1318)
                     /locus_tag="NWT83_RS26325"
                     /old_locus_tag="NWT83_26325"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            complement(1770..1985)
                     /locus_tag="NWT83_RS26330"
                     /old_locus_tag="NWT83_26330"
     CDS             complement(1770..1985)
                     /locus_tag="NWT83_RS26330"
                     /old_locus_tag="NWT83_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1992..2489)
                     /locus_tag="NWT83_RS26335"
                     /old_locus_tag="NWT83_26335"
     CDS             complement(1992..2489)
                     /locus_tag="NWT83_RS26335"
                     /old_locus_tag="NWT83_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2530..3228)
                     /locus_tag="NWT83_RS26450"
                     /pseudo=""
     CDS             complement(<2530..3228)
                     /locus_tag="NWT83_RS26450"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3167..3508)
                     /locus_tag="NWT83_RS26345"
                     /old_locus_tag="NWT83_26345"
     CDS             complement(3167..3508)
                     /locus_tag="NWT83_RS26345"
                     /old_locus_tag="NWT83_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4122..4253
                     /locus_tag="NWT83_RS26350"
                     /old_locus_tag="NWT83_26350"
     CDS             4122..4253
                     /locus_tag="NWT83_RS26350"
                     /old_locus_tag="NWT83_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     Typing          complement(3593..3768)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          3715..4231
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.076"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1655..3177)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3174..3487)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.04e-58"
ORIGIN
        1 atatttatcg aaaaatatct tttctaccaa ctgatggtat ctatttttgg acctttcttt
       61 tttatcctta ctcacaaaca acctccttgt tactattttt tattctctta gcaattacat
      121 ttgatgacac accaaacata gatgcagcat cggacataga aatagcaacc aaactttcat
      181 caccgagttt gattttcttc gttggttttt ttggcgtaac accaagaacc ttcataatct
      241 gcaagccaac acttctagct agcataggag gaacactatt accgacctgt ctaaatccat
      301 gccacttagt cacatgaaat ctgaaccaat caggatatga atgcagtctt gctgcctctc
      361 tgaccgtgat gaccctagga tagatcggat gtataggacg aggagatgta aaagcacctc
      421 tgtcacttgc tgttcctgcc cttaaagtat tacatagccc attcatcgag agctttttaa
      481 acctgctgat tggttctacc gtgtcaggca aagtttcaac aaaccgccct ctagaaatgt
      541 cagagtgatc tgtcagtaaa gaacacgtta atttgtcttt attaaaaatc cttgggtagc
      601 taaaatcact aggatctttt cctacgccat gcaaatagga tgagtatagt gattgctcac
      661 caaatttagc ataaacccaa tctctacccc atagctcagg atatttttcc gcatcaggaa
      721 tatccccaat agcgtcccca accgttgttc taatggataa tggctcaggg tatgatggca
      781 gagcaattcc tttccttccc cccattaaga aaagacgacg gcgatcttga ggaactccat
      841 aatcagctgc gtttagcact ttataatcgc ttacaacgtc gtacccgcca tctttaaatg
      901 catccattat ctctactaaa aactgcttat gcttaccaac cgtcaaccct tttacatttt
      961 caaaaacaaa gtatttaggc tttagctcaa gaaccagccg tacatagtga tagaccagtt
     1021 tgtttctatc atcatcaagc gcgcgttttc ctattaatga aaagccttgg cagggagcgc
     1081 ctccgatcac tacatcaatc tctttctcac caatacctga aacttcccta acgtattctc
     1141 cggtaacatc aacaacactt ctacatatcg ttttgcacat aggaaagtta tatttgtgag
     1201 cagagcaatg tattggatct atttcaactg atgccattat atcaaagcca gcttgctcga
     1261 aaccgagact cagcccaccc gctccagcaa ataaatcaac agcaatcggt ctagtcattc
     1321 tttacaccca cgttatataa aatcaacatg atgctatcag aaaaacgatc gctttaccga
     1381 tcgtttttct gatttcgatt gaacataaca tctcttaccc gcaccgcgcc ggaggcactc
     1441 agggcgtttc tgatggctga cgggcaaaag gtggtggcaa gtacaccgaa aaccaccaga
     1501 aacgcacctg atttatttta cgcatatgca tcacttattc acatcactgc atagccggtg
     1561 atttcggtta aaaaagactc tctgtgtgct gaaatcgcag aaaatggcca tgatccccgt
     1621 cacgctcagg ccacctgaga cgggcgttta tcagtctcca tactccctgt caccatgaga
     1681 taatgtgcgt tgccgttccg gtaccagatc tgctgtcttc tctggttcct tttccgttgt
     1741 ttgcccgtat cgctgttgct caatctcatt cagcctgtcg ataaccggcg cagctgctcg
     1801 ctcaaactct gcacctgcct gctcaagccc gacacgcgct cgctcagcgc cgcgttctcc
     1861 cgttgcataa gaccagacat cttccgccat tcctcgaagg cgctctccca ctcgtccagc
     1921 cttctcgagt agtcctgctg tagctgctct aacgcgctca gcaattgttt ttccagttcc
     1981 gtcattctgc tctactcccg taccagcttc accgcgttcc gcgtcttgtt gtccaccgtc
     2041 cggcccgttt cggctttcat cccctccggc agcaccagaa accgtccgtt gcggttctcc
     2101 agataggtca cgccccacgt ctgcatgtgc agtttcgaca gcgtatctct ctgctgcctg
     2161 atttccgcca ggttcgccgc tatccggttc ccctggtacc acagcacccc gctcaggctg
     2221 gcgaacagca gccctgacac catggtgatg gtcagccacg tccgcccgac cattcgcagc
     2281 acgctcagcc ggttcgactg catcgccgcg ttcatcccct gttcgtgggc gctgatggcg
     2341 tcgttgattc tcttcgcgct tgcgttcagt tccgatttga caacgttctc gtgcttgctg
     2401 aatgcggctt tcagcctctc ttcggtaagc tgctgctgtg ctttcgattt ctcctcgaag
     2461 tccctcgagg ccgttaaaag actgctcata aatggctccc ctcagtcgga tatttcgccc
     2521 cccgtccggg tcggcaatgc tgatactgtt ttttgtggtt ctgaccacct caaaaccggc
     2581 cgctgtgagc gcccctgtga cgtcatcacg cgttttcagc tctcctgatg aggctaacat
     2641 cagcaaaccc cgtgtaatcg tctctgcggc ttcctgcttc gtttctggca gtcctgaagg
     2701 cgtgaccagc gcccgccggt tctccgggtc gttcggatca tgtagcccca gcctgccgtt
     2761 gaccactgtc tgccacgcat cgatgcgcgg ccggtcggca cgatcgtaat acggctggag
     2821 gcgtttcccc gtcagtaatt cggtattggg gatcagaaaa ttcagctcaa gacgaccctt
     2881 gtctgcgtgt tccacccaca gaatgctgta ctggtcttta tcgagtccgg gcatcagaac
     2941 ccgctcaaag ctggccatca gtttttcacg ctgacccggc ggcaggtccg cctccgcgaa
     3001 tgacagcacc ccggaggtat attttttcac atacggcgac gcatcaatca gctcccggac
     3061 ttcttccggc ttcccctgca ggacgctggc accttcacgc tgccggtctt tacccagaag
     3121 ataatccaca ggaccggcgc ccccgccgcg accccgggga tggaatttaa caatcatcgt
     3181 ccggcccttt ttccagtacg gcatgccgca accgttccag accggcatcg atcgccatca
     3241 gcgctgccac gatctgcacc cggtcatgac cggcaccacc ggcgttcact ttccgggcga
     3301 tctggttcag gttattgccc atgccggcaa gctgacgcag cagcgcgggc gaaatcgagg
     3361 gaagcctgcc ggcgcgtgtc ggtttctcat caaggcaggt ctggcgcatc cacgccgcca
     3421 gttgttttcc gtcgcaccgc tcaagcaacc gccggtgttc gtcttccgtc acccacatcg
     3481 tgagcatctt gttgcgttta tctgccagaa tcgcctcctg ataacgccgt gacggacggg
     3541 aaagcctttc ccggtcggcg cggggttgga caagccgcag gcgcgtcagt ggggtttcgg
     3601 ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctt agtatgttac
     3661 ggcgagatgg agattcgggg aagtgcttca tgtggcaagg gggaaaatac ggctgcgggg
     3721 cgtaagtgca aaatgcaggt aacgatgaaa tgacgcctcc tcgctcactc ggtcgctgcg
     3781 ctcctgccgt gagactgcgg cgggcgttac cagctcacga aggacgagat aacgagatcg
     3841 cgctcaagtc aggaaagtca ggaaaaagga tgcggcgttg ccgtttttcc ataggctccg
     3901 cccccctgac aagcatcaca aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     3961 actataaaga tcccaggcgt ttccccctgg tagctccctc gtgcgctctc ctgttcctgc
     4021 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga
     4081 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
     4141 agtccgactg ctgcgccttg tccggtaact atcgtcttga gtccaacccg aaaagacatg
     4201 caaaagcgcc actggcagca gccactggta atcggattca cggagcggag tagagttatc
     4261 tacagagttc ttgaagtggt ggcctgacta cggctacact gaaaggacag ttttggtatc
     4321 tgcgctccgt gaaagccagt taccttcgga agaaagagtt ggtagctcta aattccggca
     4381 aacgaaccac cgttggtagc ggtggttttt ttgtttacgg cttaggagat tacggctaga
     4441 acgaaaggat ctcaagaaga tcctctcaga aattatacag acatgcggta gtttgagatg
     4501 tcagagctat ctaactcttt taccggaacc agtttgtaat gcctttcctc aatcaatttc
     4561 acttgtcctt cgtctatctt tagctcaaac agtgcaatca aatcgctcga aacaaactga
     4621 gcggaaatag ctctacatct aacatttggg tatttttgtt cacaccacgc tgtatcttgc
     4681 gatgtttgca ccattgatat ttgatctgtc ccaccttttg cttgaactgg tattatataa
     4741 tggcaaccgt atttatctat acctacataa agttcatcga tctctatttg accaattccc
     4801 ttcaccgtag ttcttaaatg attctgtaaa gaatatgtgg ttatacctag aaatatatct
     4861 attagccggt tatatctcac cttcgccagc aatgcctgct cgtcgtctaa agaataacct
     4921 gtaattatct ccggggtatt gtctggaatt tttattgcca ccatattagg atttggcaaa
     4981 atcctactaa tagttactaa tctaaatcga tactttgacc taccagccag ctcaataatc
     5041 cactccttac cttctggctg agtcaacaga atactttctg gcataggcgt tctaaatctg
     5101 attgaatata taacatcacc aacattagat gggagttcta tacctaaatc ttttgcagca
     5161 ctgtttatat ctaacctttc aaacaaaacc tctaagtcac catcaac
//
