LOCUS       NZ_CP102897             4513 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella quasipneumoniae strain KA1 plasmid pKA1-4, complete
            sequence.
ACCESSION   NZ_CP102897
VERSION     NZ_CP102897.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054357
            Assembly: GCF_024734235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-AUG-2022
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 389.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:07:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,403
            CDSs (total)                      :: 5,283
            Genes (coding)                    :: 5,100
            CDSs (with protein)               :: 5,100
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 61 of 183
            Pseudo Genes (incomplete)         :: 119 of 183
            Pseudo Genes (internal stop)      :: 35 of 183
            Pseudo Genes (multiple problems)  :: 28 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102897.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KA1"
                     /host="animal"
                     /db_xref="taxon:1463165"
                     /plasmid="pKA1-4"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            complement(join(4289..4513,1..894))
                     /locus_tag="NWT75_RS26960"
                     /old_locus_tag="NWT75_26960"
     CDS             complement(join(4289..4513,1..894))
                     /locus_tag="NWT75_RS26960"
                     /old_locus_tag="NWT75_26960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="WP_151085616.1"
                     /translation="MKRTMHNIGDTDKENKGEMNISLYNFKKAEGVQEKIIHIKKTQFK
                     NKLVNFFWNTSKNEVLKGMGKVSSISDFHSTAHALSYGSDTSEIKNRTEIQLGDSVFYY
                     KKLSVDNESFVFGYVTGIFRKNNIESIEITRTSLDKTINRSQIKLILEKNTPIFLLSNG
                     YNDRYEKLAPTNNKLKIMYKYQLDSLEFVLGNVRLLHLIKNRYSNRQIPLGELAFKEIH
                     KSMFGDIHHWAGEYRNHEVIVGDRGRETVHQTEISKCMKTAFQKTHKNRLSSINNIDSL
                     SNMLTILHKDLAWIHPFEDGNGRAIRFYLLILSLSLGFKMDLKVFIGDKKRKSFYHYAV
                     RKAIYDNNNKYLHRIIKNSLTMISHNTNSQKN"
                     /name="Fic family protein"
     gene            1910..2101
                     /locus_tag="NWT75_RS26965"
                     /old_locus_tag="NWT75_26965"
     CDS             1910..2101
                     /locus_tag="NWT75_RS26965"
                     /old_locus_tag="NWT75_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_258336618.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEAGLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2101..2502
                     /locus_tag="NWT75_RS26970"
                     /old_locus_tag="NWT75_26970"
     CDS             2101..2502
                     /locus_tag="NWT75_RS26970"
                     /old_locus_tag="NWT75_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_258336619.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMLDTSE
                     LLRVFGPFEQPKGEHHSVQVSEQRDTASADQSEQVIRHLRQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3344..3553)
                     /locus_tag="NWT75_RS26975"
                     /old_locus_tag="NWT75_26975"
     CDS             complement(3344..3553)
                     /locus_tag="NWT75_RS26975"
                     /old_locus_tag="NWT75_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_073511423.1"
                     /translation="MKSIKTFVAVTALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     Typing          complement(1390..1760)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.834"
                     /coverage="91.0"
                     /evalue="4.68e-157"
ORIGIN
        1 gaatggatga atccatgcca aatctttatg caggattgtg agcatgttgg ataaggagtc
       61 aatgttattt atgctgctta acctgttttt atgtgttttt tgaaaggctg tcttcatgca
      121 tttacttatc tctgtttggt gcaccgtctc cctgccccta tcaccaacta taacttcatg
      181 attacggtat tccccggccc aatgatgaat gtccccaaac attgatttat gtatttcctt
      241 aaatgctaac tcacccaatg gtatttgtct gttactgtat ctattcttaa ttaaatgcaa
      301 tagccgcaca ttccccaaaa caaactcaag gctatctagt tgatatttat acattatttt
      361 tagcttgttg tttgttgggg ctagtttttc atatcgatca ttatatccat ttgataataa
      421 gaatattggt gtgttttttt ctaaaatcaa tttaatttga cttctgttta ttgtcttatc
      481 aagagatgtt cttgttattt caatagactc aatgttgttt tttctgaata ttccagtgac
      541 atacccaaag acaaatgatt cattatccac actcaatttt ttgtagtaaa aaacagagtc
      601 tccaagctgt atctctgttc tgttttttat ttcactagta tcactaccgt aggaaagtgc
      661 atgagctgta ctgtgaaagt cacttatgct actaacttta cccatgcctt ttagaacttc
      721 atttttacta gtgttccaaa agaaattaac caatttattc ttgaattgcg ttttttttat
      781 gtgaattatt ttttcttgaa caccttctgc ctttttaaag ttgtataaag atatgttcat
      841 ttcaccttta ttttctttgt ctgtatcacc aatgttatgc attgttcttt tcataattaa
      901 aatcgccggt attaatctga agtgccatgt aatcttatag ataatatctt ataaaaaagg
      961 ttgttcacaa tagcatctct ttttgccatg caacggcata gcttaataac tcatcaaaaa
     1021 taagtgtagg aggagctcat ggagcatgag aacacgcttt ttcatattac ggattcatct
     1081 atatttttaa ttaaatcatg tggttatgtt gagtctttaa gtcattggtg atgatgtgta
     1141 gtgggtggat gggcttaacg tctgtgtgag atctaacaga tttacttgtg atcctttccg
     1201 tggcgtcgta gtctcttggc ctgtaaacga aaaaaccacc ctggcaggtg gtttttcgaa
     1261 ggttagttaa tcctggcaga ttatctaacc gtggtaactg gctttcgcag agcacagaaa
     1321 ccaaattagt tatagcagtg tagctgtagt tagccccaca cttcaagact tctttgttct
     1381 gcgaacctgt taccagtggc tgctgccagt ggcgttttgt cgtgtctttc cgggttggac
     1441 tcaagatgat agttaccgga taaggcgcag cagtcggact gaacgggggg ttcgtgcaca
     1501 cagtccagct tggagcgaac tgcctacccg gagctgagtg tcaggcgtgg aatgagataa
     1561 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     1621 gagggagcca tcagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     1681 ctgatttgag tgtcagattc tgtgatactt gtcagggggg cgaagcctat ggaaaaacag
     1741 ccgcgtggcg gcttttcctt ttctccctta tacgtctggt tacctactct tttgtgtata
     1801 tgcccctgcc gctcgccgca gccgaacgac tggagcgtag cgactgagtg agcgaggaag
     1861 cggaaaagag actggttcta cactgagcac tgacgctctg aggcttctta tgactaaaca
     1921 acagaaaaca gcgctgaaca tggctaaatt catccagcat cagtctctgc tcctgctgga
     1981 gaagcttaac gagcttgatc ttgatgctga ggccggcctt tgtgagaaac tccacgacga
     2041 tgcggagcat cttttccgta cactgtcctc gagactggat gcattacagg atggtaactg
     2101 atggccagag tgtctattag tgaggctgcc cgactgacgg gtaagagtag aacaacactg
     2161 cacaggttaa ttaaagcagg tgacctgtct acctgtagcg gtgagcgtaa tgcaaaaatg
     2221 ttagatactt cagagctttt acgtgtcttt ggtccctttg agcaaccgaa gggtgaacac
     2281 cattctgtac aggtctctga acagcgtgat acagcctctg ctgaccagag tgaacaggtg
     2341 attcgtcatc tgagacagga gattgaacac ctgaacacct tacttttgtc caaggattcg
     2401 cacattgaca gcctgaaaca ggccatgctc cttcttgaaa atcaccaggc aaaattaact
     2461 gaatccccgg cgccatggtg gaaattctgg aaaaaatcct gaccctgtca tgcttcagtt
     2521 ttcctctccg gtcattccgg ggagttcctg aaccttccca aaaatcgtca gatctctgaa
     2581 cgatcctcac tactaaaaat acccacaccc cacgcaaaat taaaattttg aagataagtc
     2641 attaatatca gtcagttgtg ggttattaaa ttacgcagta ttactttctg taaggtgaat
     2701 ccaagtcgtc ggcgattcgt gtacgacttg ccgacgactc gtcatagagt catcggcgac
     2761 tcacttaaga ctatcagtaa cttatgacga cttgactcta gagtcgaatc gtcattttgg
     2821 tgtttttttg accgtagcga ctctagagtc aagtcacgag tcgaatcgcc gatgaatcgt
     2881 gtggtgagtc gtacggcgac ttgccataac gtcattgccg gccgtcgacg aacccgattc
     2941 ctgagtgtgc tgaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
     3001 attgcgtccc cgttcaccgt tttcggatca gaagaacgtc ggaaacagga cttaccctga
     3061 ctgaatgggg tgaattttcg cgctatgcac ttgtgtgcat actcatgcat gccgtaaaaa
     3121 cagagcctgc gtatttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
     3181 cgtcagaaac gtcctgaggc cgttttagcg gtgcgtacaa tcatgccttt atgttaaatg
     3241 gcaggtgcaa aaaagccccc gaaggggcct gagggttttc ctgctaaggg acgaaacggg
     3301 ccgcctcccg gcgacctgtt cggcagctcc ctctgcgacc atcttatttg gtcagttcag
     3361 ccgtcatgtg gacacggtta ttgaactgag cgctggtgat tttgtatgac gcaccttgtt
     3421 cagcggcctg tgcggcaatt tttgcttcag cacgatccag ggtggaggcg gtagcgctga
     3481 cgctctgagc aaaggcaccg aaagacatca tggatagagc ggtaactgca acgaaagttt
     3541 tgattgattt catggtcgta ttccttaagt cattttgttc tgtatggggc gttgttgccc
     3601 tgatgtgata aataataggg cgacaatgag ctgattaaaa tcgaagcaat ttgcggatat
     3661 tattcaaaaa aatagaacaa aaattgagca caccaaaacc agatgtgaga ctctgattta
     3721 ggggcaggat gaatagcctc gagggggggg ggataacttt gtcgaccctc tttagagagt
     3781 tagctgccat ttatatatgc gtacttattg gctgataatt gctgattttt tatggcgtga
     3841 tgcatttgag taatcatgct aactatttgt taaaatttaa ttttctttgc tatatgaatt
     3901 gcatgatggg ctatgcccta tttgtttagt agcaataatg agattacaac cctctcgtta
     3961 tgatgatata ttttagctat tgactttttg cgaaattcat gataatgtaa ggccactgac
     4021 gtaccggggc gctgtagttc actacagcgc cccggtacgt cagtggctta gtgctttaag
     4081 tcgttgatta ttatgctttt ttataaccct aggcggataa ctatgtcaca ttgtttggaa
     4141 tcagtagcct tcgagttgag aaatgatgat caatcattct ctatggaagt tggtgagttg
     4201 attggtttca ttcatgactc tctgctgtca tcattgtatc tggaaggcga tgtaatcaac
     4261 atgaatgaaa gtgagtctaa aagacttatt agttcttttg agaattcgta ttatgggata
     4321 tcatagttag agagtttttt attatcctat gtagatattt attattatta tcgtaaatcg
     4381 cctttcttac cgcatagtgg taaaatgatt tacgcttttt gtctccaatg aacactttta
     4441 agtccatttt aaatccgaga gatagcgata ggattaataa ataaaatctt atcgctctcc
     4501 catttccatc ttc
//
