LOCUS       NZ_CP102888             3494 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KH2 plasmid pKH2-5, complete sequence.
ACCESSION   NZ_CP102888
VERSION     NZ_CP102888.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054360
            Assembly: GCF_024734175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.0x
            Sequencing Technology             :: PacBio; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:20:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,754
            CDSs (total)                      :: 5,630
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 49 of 146
            Pseudo Genes (incomplete)         :: 94 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102888.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KH2"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKH2-5"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            257..415
                     /locus_tag="M2890_RS28795"
     CDS             257..415
                     /locus_tag="M2890_RS28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071819240.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKTPLAAAIGIWFTERRGV
                     YKHTQSS"
                     /name="replication initiation protein"
     gene            complement(604..2136)
                     /locus_tag="M2890_RS28730"
                     /old_locus_tag="M2890_28730"
     CDS             complement(604..2136)
                     /locus_tag="M2890_RS28730"
                     /old_locus_tag="M2890_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_110917701.1"
                     /translation="MKKSVVRQVLEMSGPCISSDLAERIQWQHPSMSPEAIRKMISRST
                     DIGKLPFLKFSHNRRFIYLKDDFGSFKFWRALEKCMYEANSTYSHAILAVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYETVLRNLLSAKILRAVYIDGVGDCVLINNNIANDVNIRTM
                     ANCESFFDKPILELVKAWLRNLGLVAFNQIKTKYDGEGNPVVGSFEWDMTAPSYVSPLA
                     EYVGGKLMPGFVACDFSLGFNRDEITTAAAETFIRKVQMTKSSRASQRIMFVIFARRFG
                     KIAFNKLRSEGVLAVTIANAFGNKVDESLTRLSRVVQGSLSIEKHPDELLQMVKDLESV
                     SGENGNLRGYIFELFVSSQISNFYGLGNIYINREYKINGKHAEADVVLESSDDIYIIEC
                     KNVKVLPSTELTRWMKERVPTINSYYKINNLEKKNIHHYLWVTGNVYNNDLKRLDSFKK
                     NNKSIDVDYLFGEHLDDFFYNKKRVFNLYRKVISPDYKKGVMPDFELEDDFF"
                     /name="NERD domain-containing protein"
     gene            complement(3142..3333)
                     /locus_tag="M2890_RS28735"
                     /old_locus_tag="M2890_28735"
     CDS             complement(3142..3333)
                     /locus_tag="M2890_RS28735"
                     /old_locus_tag="M2890_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          77..556
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.758"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          3209..3432
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.667"
                     /coverage="85.0"
                     /evalue="1.71e-93"
ORIGIN
        1 gccacgcccc ggcggggcgt ttttccatag gctccgcccc cctgacaagc atcaagaaat
       61 ctgacgctca aatcagtggt ggcgaaaccc gacaggactt aaagataccg ggcgtttccc
      121 cccggtggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcactccg
      181 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
      241 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttatccgg
      301 taactatcgt cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
      361 tggtatctgg tttacggagc ggagaggcgt gtacaagcat acacagagtt cttgaagtgt
      421 tgacctgact acggctacac tggaaggaca gatttggtca ctgcgctccg tttaagccag
      481 ttaccacggt tcagaaactt gccggtttcc tgaaccttcg aaaaaccacc ttgccgggtg
      541 gttttttcgt ttcagagatg gaagatacgc ctttcaaagc gctatcgtca agaaacgcag
      601 tagctagaaa aaatcgtcct ctaactcaaa atcaggcatt acgccttttt tataatcagg
      661 tgaaatgact tttctatata agttgaacac ccgcttttta ttgtaaaaga aatcatccaa
      721 atgctcacca aacaaataat caacatcgat actcttatta tttttcttaa aagagtctaa
      781 cctttttaaa tcgttgttgt acacattacc tgtaacccac aaataatggt ggatattctt
      841 tttttctaaa ttatttattt tatagtaaga gtttatagtt ggaacccttt ctttcatcca
      901 acgcgtaagt tcagttgaag gtaagacctt aacattcttg cactctatta tataaatatc
      961 gtcactactc tctaatacaa catcagcttc tgcatgctta ccatttatct tatactctct
     1021 attaatgtaa atattaccaa gaccataaaa attagaaatt tgcgaggata caaaaagctc
     1081 aaaaatatac cctctcaaat tgccattctc tccagaaaca gactcaagat ctttaaccat
     1141 ctgaagcaat tcatctggat gcttttcaat ggacaaagaa ccctgaacca ccctcgacaa
     1201 cctggttaag ctttcatcaa ctttattacc aaacgcattt gctatagtta cagccagaac
     1261 cccctcactt ctcaacttat tgaaggctat tttgccgaaa cgccttgcga aaataacaaa
     1321 cattatcctc tgactcgctc gagaagactt ggtcatctgg acttttctaa tgaatgtttc
     1381 tgctgcagcg gtagtaattt catcacgatt aaagccgaga gaaaaatcac aggcaacgaa
     1441 accaggcatc agttttccgc cgacatattc agcgagaggg gaaacgtaac ttggagcggt
     1501 catatcccat tcaaaagagc ctacaactgg atttccctcc ccatcgtatt ttgtttttat
     1561 ctgattaaaa gcgactaggc ctaaattcct caaccaagct ttaacaagct ccaatattgg
     1621 tttgtcaaaa aaggattcgc aatttgccat tgttctaata ttaacatcgt tggctatgtt
     1681 gttatttatc agtacgcaat caccaactcc atcaatatac acagctctaa gtatttttgc
     1741 cgaaagtaga tttcttagaa ctgtttcata acttaaatgt ttagcttgtt ttataggaga
     1801 tccagacaca atcccaaaat ctttcacctt caaataaccg ccattattta ttacggcaag
     1861 tatcgcgtgt gagtaagttg agtttgcttc gtacatgcac ttttcgagag ctcgccaaaa
     1921 tttgaaactg ccaaaatcat cttttagata aatgaatctc ctgttatggc tgaatttcaa
     1981 aaatggcagc ttacctatgt ccgtgcttcg cgagatcatt ttacgtatcg cttcagggga
     2041 cattgaggga tgttgccact gaattctctc tgcaagatct gaagagatgc atggcccgct
     2101 catttcaagc acttgtctaa caacactttt tttcatagcc tcaccttatt tctgtcacac
     2161 tacgctattt ataacattac actacgtgta ataacgcgca gtctttttaa aaaaatgact
     2221 ataaaaaaca attgaaaatc agatagttaa ttacttaatt taaattaagc acaaagaaat
     2281 atcgtcttta taattattta atccttttaa atcataaaga tagctttgtg acattttttt
     2341 atcgaactat cagctactcc atttaccata acatccgtta tcagcaccgc ctcagaggcc
     2401 ctcagggcgt ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc
     2461 agaaacgcgc aggctctgtt tttacggcat gcatgagtat gcgcacaagt gcatagcgcg
     2521 gaaattcacg ccattcagcc ggggtaagtc ctgtttccga tgttcttctt acccgaaaac
     2581 ggtgaacggg gacgcaattt attgcgtggc cgtgaggcgt tgccggcgca gccggaaaac
     2641 ccgttttcag cacgttcagg aagggggtga tgtacaaatg gcacagagga tggaagtgac
     2701 gtatgagctg aaggaatgat gtaaaagtga tgtaaaagtg atgtaaaagt gatgcaaaag
     2761 tgttgtaaaa gtgatgtaca gagcgaagga gtgttgtaaa agtgttgtaa aagtgatgta
     2821 tgacctgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
     2881 aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc
     2941 cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca gctgcgattt
     3001 tcatcgcaac ttcatgaaaa ataaataaaa aagaaaaaat acagggattc tgggtattac
     3061 cggggtaata atattaagcg ctaacacaat gaaaattaag gcgatgccgg tattactccg
     3121 gtaataccca gaacggagcc attacgcacc gtcctgctgc tcattcaggc gcgctgacag
     3181 gcttcggtaa aggcgttccg catcctcatg gagtttctcg cagaaatcag cctcatgatc
     3241 cagatccagc tcattaagcc tgtccagaag aagcagggac tgatcctgga tgaatttagc
     3301 catgttaacg gcagtctttt gttgtttgtt catgaaggcc ccagcgcgaa agtgcttact
     3361 gtacgttctg atggttttcc gcttcctcgc tcattcgctc actccgttcg gtcatgagtc
     3421 tgcggcgagc ggagggaaga taagcaggta acgtaacggt aagcataacg aaagctaagg
     3481 acaagggcga aaag
//
