LOCUS       NZ_CP102882             3494 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KH1 plasmid pKH1-5, complete sequence.
ACCESSION   NZ_CP102882
VERSION     NZ_CP102882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054359
            Assembly: GCF_024734155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-AUG-2022
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 252.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:16:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,754
            CDSs (total)                      :: 5,630
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 49 of 146
            Pseudo Genes (incomplete)         :: 94 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKH1-5"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            complement(8..199)
                     /locus_tag="NWT87_RS28730"
                     /old_locus_tag="NWT87_28730"
     CDS             complement(8..199)
                     /locus_tag="NWT87_RS28730"
                     /old_locus_tag="NWT87_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            617..775
                     /locus_tag="NWT87_RS28795"
     CDS             617..775
                     /locus_tag="NWT87_RS28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071819240.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKTPLAAAIGIWFTERRGV
                     YKHTQSS"
                     /name="replication initiation protein"
     gene            complement(964..2496)
                     /locus_tag="NWT87_RS28735"
                     /old_locus_tag="NWT87_28735"
     CDS             complement(964..2496)
                     /locus_tag="NWT87_RS28735"
                     /old_locus_tag="NWT87_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_110917701.1"
                     /translation="MKKSVVRQVLEMSGPCISSDLAERIQWQHPSMSPEAIRKMISRST
                     DIGKLPFLKFSHNRRFIYLKDDFGSFKFWRALEKCMYEANSTYSHAILAVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYETVLRNLLSAKILRAVYIDGVGDCVLINNNIANDVNIRTM
                     ANCESFFDKPILELVKAWLRNLGLVAFNQIKTKYDGEGNPVVGSFEWDMTAPSYVSPLA
                     EYVGGKLMPGFVACDFSLGFNRDEITTAAAETFIRKVQMTKSSRASQRIMFVIFARRFG
                     KIAFNKLRSEGVLAVTIANAFGNKVDESLTRLSRVVQGSLSIEKHPDELLQMVKDLESV
                     SGENGNLRGYIFELFVSSQISNFYGLGNIYINREYKINGKHAEADVVLESSDDIYIIEC
                     KNVKVLPSTELTRWMKERVPTINSYYKINNLEKKNIHHYLWVTGNVYNNDLKRLDSFKK
                     NNKSIDVDYLFGEHLDDFFYNKKRVFNLYRKVISPDYKKGVMPDFELEDDFF"
                     /name="NERD domain-containing protein"
     Typing          437..916
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.758"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ggagccatta cgcaccgtcc tgctgctcat tcaggcgcgc tgacaggctt cggtaaaggc
       61 gttccgcatc ctcatggagt ttctcgcaga aatcagcctc atgatccaga tccagctcat
      121 taagcctgtc cagaagaagc agggactgat cctggatgaa tttagccatg ttaacggcag
      181 tcttttgttg tttgttcatg aaggccccag cgcgaaagtg cttactgtac gttctgatgg
      241 ttttccgctt cctcgctcat tcgctcactc cgttcggtca tgagtctgcg gcgagcggag
      301 ggaagataag caggtaacgt aacggtaagc ataacgaaag ctaaggacaa gggcgaaaag
      361 gccacgcccc ggcggggcgt ttttccatag gctccgcccc cctgacaagc atcaagaaat
      421 ctgacgctca aatcagtggt ggcgaaaccc gacaggactt aaagataccg ggcgtttccc
      481 cccggtggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcactccg
      541 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
      601 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttatccgg
      661 taactatcgt cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
      721 tggtatctgg tttacggagc ggagaggcgt gtacaagcat acacagagtt cttgaagtgt
      781 tgacctgact acggctacac tggaaggaca gatttggtca ctgcgctccg tttaagccag
      841 ttaccacggt tcagaaactt gccggtttcc tgaaccttcg aaaaaccacc ttgccgggtg
      901 gttttttcgt ttcagagatg gaagatacgc ctttcaaagc gctatcgtca agaaacgcag
      961 tagctagaaa aaatcgtcct ctaactcaaa atcaggcatt acgccttttt tataatcagg
     1021 tgaaatgact tttctatata agttgaacac ccgcttttta ttgtaaaaga aatcatccaa
     1081 atgctcacca aacaaataat caacatcgat actcttatta tttttcttaa aagagtctaa
     1141 cctttttaaa tcgttgttgt acacattacc tgtaacccac aaataatggt ggatattctt
     1201 tttttctaaa ttatttattt tatagtaaga gtttatagtt ggaacccttt ctttcatcca
     1261 acgcgtaagt tcagttgaag gtaagacctt aacattcttg cactctatta tataaatatc
     1321 gtcactactc tctaatacaa catcagcttc tgcatgctta ccatttatct tatactctct
     1381 attaatgtaa atattaccaa gaccataaaa attagaaatt tgcgaggata caaaaagctc
     1441 aaaaatatac cctctcaaat tgccattctc tccagaaaca gactcaagat ctttaaccat
     1501 ctgaagcaat tcatctggat gcttttcaat ggacaaagaa ccctgaacca ccctcgacaa
     1561 cctggttaag ctttcatcaa ctttattacc aaacgcattt gctatagtta cagccagaac
     1621 cccctcactt ctcaacttat tgaaggctat tttgccgaaa cgccttgcga aaataacaaa
     1681 cattatcctc tgactcgctc gagaagactt ggtcatctgg acttttctaa tgaatgtttc
     1741 tgctgcagcg gtagtaattt catcacgatt aaagccgaga gaaaaatcac aggcaacgaa
     1801 accaggcatc agttttccgc cgacatattc agcgagaggg gaaacgtaac ttggagcggt
     1861 catatcccat tcaaaagagc ctacaactgg atttccctcc ccatcgtatt ttgtttttat
     1921 ctgattaaaa gcgactaggc ctaaattcct caaccaagct ttaacaagct ccaatattgg
     1981 tttgtcaaaa aaggattcgc aatttgccat tgttctaata ttaacatcgt tggctatgtt
     2041 gttatttatc agtacgcaat caccaactcc atcaatatac acagctctaa gtatttttgc
     2101 cgaaagtaga tttcttagaa ctgtttcata acttaaatgt ttagcttgtt ttataggaga
     2161 tccagacaca atcccaaaat ctttcacctt caaataaccg ccattattta ttacggcaag
     2221 tatcgcgtgt gagtaagttg agtttgcttc gtacatgcac ttttcgagag ctcgccaaaa
     2281 tttgaaactg ccaaaatcat cttttagata aatgaatctc ctgttatggc tgaatttcaa
     2341 aaatggcagc ttacctatgt ccgtgcttcg cgagatcatt ttacgtatcg cttcagggga
     2401 cattgaggga tgttgccact gaattctctc tgcaagatct gaagagatgc atggcccgct
     2461 catttcaagc acttgtctaa caacactttt tttcatagcc tcaccttatt tctgtcacac
     2521 tacgctattt ataacattac actacgtgta ataacgcgca gtctttttaa aaaaatgact
     2581 ataaaaaaca attgaaaatc agatagttaa ttacttaatt taaattaagc acaaagaaat
     2641 atcgtcttta taattattta atccttttaa atcataaaga tagctttgtg acattttttt
     2701 atcgaactat cagctactcc atttaccata acatccgtta tcagcaccgc ctcagaggcc
     2761 ctcagggcgt ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc
     2821 agaaacgcgc aggctctgtt tttacggcat gcatgagtat gcgcacaagt gcatagcgcg
     2881 gaaattcacg ccattcagcc ggggtaagtc ctgtttccga tgttcttctt acccgaaaac
     2941 ggtgaacggg gacgcaattt attgcgtggc cgtgaggcgt tgccggcgca gccggaaaac
     3001 ccgttttcag cacgttcagg aagggggtga tgtacaaatg gcacagagga tggaagtgac
     3061 gtatgagctg aaggaatgat gtaaaagtga tgtaaaagtg atgtaaaagt gatgcaaaag
     3121 tgttgtaaaa gtgatgtaca gagcgaagga gtgttgtaaa agtgttgtaa aagtgatgta
     3181 tgacctgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
     3241 aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc
     3301 cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca gctgcgattt
     3361 tcatcgcaac ttcatgaaaa ataaataaaa aagaaaaaat acagggattc tgggtattac
     3421 cggggtaata atattaagcg ctaacacaat gaaaattaag gcgatgccgg tattactccg
     3481 gtaataccca gaac
//
