LOCUS       NZ_CP102876             4473 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KA2 plasmid pKA2-7, complete sequence.
ACCESSION   NZ_CP102876
VERSION     NZ_CP102876.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28054358
            Assembly: GCF_024734135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Yang,C.
  TITLE     Dissemination of blaNDM-5 and mcr-8 in carbapenem-resistant
            Klebsiella pneumoniae in an animal breeding area in Eastern China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Yang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) Environment and Health, School of Public
            Health, Cheeloo College of Medicine, Shandong University, Baotuquan
            street, Jinan City, Shandong Province 250000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-AUG-2022
            Assembly Method                   :: Unicycler v. v0.5.0 OR Aug-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 246.0x
            Sequencing Technology             :: PacBio Sequel; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/09/2023 05:34:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,982
            CDSs (total)                      :: 5,856
            Genes (coding)                    :: 5,636
            CDSs (with protein)               :: 5,636
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 90 of 220
            Pseudo Genes (incomplete)         :: 131 of 220
            Pseudo Genes (internal stop)      :: 39 of 220
            Pseudo Genes (multiple problems)  :: 32 of 220
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102876.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KA2"
                     /host="animal"
                     /db_xref="taxon:573"
                     /plasmid="pKA2-7"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2019-07"
     gene            complement(join(4261..4473,1..846))
                     /locus_tag="NWT85_RS29820"
                     /old_locus_tag="NWT85_29820"
     CDS             complement(join(4261..4473,1..846))
                     /locus_tag="NWT85_RS29820"
                     /old_locus_tag="NWT85_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(1176..1342)
                     /locus_tag="NWT85_RS29935"
                     /pseudo=""
     CDS             complement(1176..1342)
                     /locus_tag="NWT85_RS29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1758..1949
                     /locus_tag="NWT85_RS29825"
                     /old_locus_tag="NWT85_29825"
     CDS             1758..1949
                     /locus_tag="NWT85_RS29825"
                     /old_locus_tag="NWT85_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_064185167.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELNLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1949..2350
                     /locus_tag="NWT85_RS29830"
                     /old_locus_tag="NWT85_29830"
     CDS             1949..2350
                     /locus_tag="NWT85_RS29830"
                     /old_locus_tag="NWT85_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064190317.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHRSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3125..3556)
                     /locus_tag="NWT85_RS29835"
                     /old_locus_tag="NWT85_29835"
     CDS             complement(3125..3556)
                     /locus_tag="NWT85_RS29835"
                     /old_locus_tag="NWT85_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_064185166.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITIDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            3974..4231
                     /locus_tag="NWT85_RS29840"
                     /old_locus_tag="NWT85_29840"
     CDS             3974..4231
                     /locus_tag="NWT85_RS29840"
                     /old_locus_tag="NWT85_29840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065953843.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEIVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     Typing          complement(1235..1637)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="99.0"
                     /evalue="3.51e-178"
ORIGIN
        1 tgagctccca tttgggtgaa tacaaaacag acttggtttt gtttgccata ctttccaaat
       61 ttcatcacca atttcggtta ctaccgtctt aaatgcactg gcagatgaat tgttcatttg
      121 cgttaatcta tttccaacat acatatatat ttgtggtagc gacatagaat aacgattgct
      181 attaagatag aaatctgatt gtggttcatt ctgatggcga cgctcaccat aaactaaaga
      241 aagtacagcc ttaactgctc tctttgatga accatcggca gagaaaggaa ttaaaaggcg
      301 ctctgctgcg gttaaagcta actctgtata aatagagaag ctagggttgc agtcaatgaa
      361 tacgcagcta tcttcattat tccatgagtt gcaaatgtca ctgattaaat cattaatcca
      421 gaggtgaaca gtacgccatg catcatgggg acctggaaat gatgccccaa caactcttga
      481 tgcttggagt tctaattgtt catcgccagt gacaagatat agattatttg gaactttact
      541 attgtgtgta gatacattta ttacatagct agaaccagtg ttggtaggaa tatatggact
      601 tctgatccta tcctcaatat atccagatat tgtctttctc tgtattgctg aatgaatctg
      661 gtttagtgtg ttttcgccat ttatcattcc accaagtaac atagaggacg agttcgcttg
      721 tgggcacagg tcaaccacca ggaccttttt agtagggttc tgtctggcat attcagaggc
      781 taactgaaaa gtaaggtaac ttttccctac accacccttg ttattccata cggcatagat
      841 cgccatctat aactcctaat ctagttttgt tgttagctta gattcagggt actagagatt
      901 attgttctac tgttagtttc tttcagctga ctgccttttt ttggaacgat attcagtcga
      961 aggggttggc tggctcacca tgacttatct ggagttgtcg aattccgatt aaaaggatct
     1021 tcttgagatc ctaacctgcg ggatgtaatc ttttgctctg taaacgaaaa aaccacctgg
     1081 ggaggtggtt tttcgaagga tcagtaagtt ggggaacttc tgaaccgtgg taacagggtg
     1141 tacaagaccg ctgccaccaa atctgtcctt tcagtttagc tgtagttagg ctgtaacttc
     1201 aagaactctg caccagtaat tcttgtacac cccttaccag tggctgctgc cagtggcgtt
     1261 ttgtcgtgcc ttccgggttg gactcaagtc gaaagttacc ggaacaggcg cagcagtcgg
     1321 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     1381 gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1441 aaggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg atatctttat
     1501 agtcctgtcg ggtttcgcca ccactgattt gagcgtcagc ttctgtgatg cttgtcaggg
     1561 gggcggagcc tatggaaaaa cggccgctaa gcggcctttc cttttccccg tcacgcgacc
     1621 gctcggtttt ctcgagcttt tacacgcatg ctcctgccgc tcgccgcagc cgaacggcag
     1681 gagcatagcg actgagtgag cgaggaagcg gaaaagagaa catccctgca ctgagcactg
     1741 acgctctgag gcctcttatg acgaaacaac agaaaacagc gctgaacatg gctaaattca
     1801 tccagaatca gtctctgctc ctgctggaga agcttaacga gcttaatctt gatgtagagg
     1861 ccgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca ctgtcctcga
     1921 gactggatgc attacaggat ggtaactgat ggccagagtg tctattagtg aggctgcccg
     1981 actgacgggt aagagtagaa caacactgca caggttaatt aaagcaggtg acctgtccac
     2041 ctgtagcggt gagcgcaatg caaaaatggt agatacttca gagcttttac gtgtctttgg
     2101 cccctttgag caaccgaagg gtgaacaccg ttctggacag gtctctggac agcgtgatac
     2161 agcctctgct gaccagagtg aacaggtgat ccgtcatctg aaacaggaga ttgaacacct
     2221 gaacacctta cttttgtcca aggattcgca cattgacagc ctgaaacagg ccatgctcct
     2281 tcttgaaaat caccaggcaa aattaactga atccccggcg ccatggtgga aattctggaa
     2341 aaaatcctga ccctgtcatg cttcagtttt cctctccggc catcccggcg agttcctgaa
     2401 ccttcccaaa aatagtcaga tctctgaacg ctcctcacca ctaaaaatac ccacacccca
     2461 cgcaaaatta aaattttgca gatttgtatt taatatcagt agtttacttt tctgaaattt
     2521 cgttttttaa attttattta tatagatccg actctatgac gactcgtcat agagtccccg
     2581 gcgagtcgtc atagagtcgg taacgactca gggctataaa aacaatgagt taataacgac
     2641 ccgactctag acccgactcc ccgtttggtt gttttttagt cgcggcgact ctaggtacga
     2701 ctctagtgca gagtcgccgg tgaatcgtgt ggtgagtcgt ccggcgactt gccataacgt
     2761 gattgccggc cgtcgacgaa cccgattcct gaacgtgctg aaaaccggtt ttccggctgc
     2821 gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt ttcggatggg
     2881 aagaacgccg gaaacaggac ttaccctgtc tgaatggcgt gaatattcgc gctatgcact
     2941 tgcgtgcata ctcatgcatg ccataaaaac agagcctgcg cgtttctggc gggttttcgg
     3001 gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg ccctcagcgc gtttctgacg
     3061 atgctgataa cggatgttat ggtaaattcg tctgtgaagt aggtagacat ttctaaagct
     3121 agttttaata gccaagtctc ttcaattcag tcgagaggcg tttgaggctt acatcaagat
     3181 tgcttttatt ttgttttaac tcttttttca taaactcagt gtgatgtgct agctcagggt
     3241 tatcagttga gcgagtcacg ggaaaatcat cactggatga aagtcgccca tttttactgt
     3301 agtctatggt gatacagatc agagttgcaa ggtcgattac ccggtgaaga ggcagctctt
     3361 ctgattgtct tgaccatttt tctcctgtgt ttcgccaaac ctttgccgaa agctcagtcc
     3421 atccaggagt gttccattga gcaataccta cagatagccc catagcatca gtgtcatcgg
     3481 catgtgggcc gtcaatgcgg tcgtaatttt cgacttgaat aattggttta tgcttcaaat
     3541 gcgcgggtat tttcatcagt tcgtccttag gtaatgtttg tgattattaa attacattgt
     3601 ttttctaatt ataaaactca ctaaatgtaa gtaaatatta atatctattg ttatcatgtg
     3661 cttgtgattt tgtttttcca attttatgtt ttttgcttgg agagtagcgg tgtttttttg
     3721 tgtcaagtgt tatgataagc cgtacgtttt ttttctggtt tatagttcgg ttattcacag
     3781 caatgggcgt ggttttctat gactacagat aacgttagga atattggcga ctataaaaag
     3841 aaagatgtcg gggtggatgc tgactgtaat ccagtctcag gaaacacacc accagtcaga
     3901 ctaagtgaga aacttagaaa tggaattcct cctggtttga aaactcagat gcgccaacaa
     3961 atgaggaaaa aacatgggct ctaacaataa tcagaataaa gatctgcagg ataagcaagc
     4021 tgatcttttg gaccaaacgt atactcttgg ttggatggat ggctatgccg aagggtatga
     4081 tgatgctatt gaagatactc agaacgcttt gaataaactg gaaaacgaaa acgagttgct
     4141 tactgagatt gttgaaagta aagataatgt aatcaaagag ttggcctcta gtgctggcag
     4201 ggcgctgcaa gcagtaaaga aaaacaaata aaaaaaggcc cttttaaggg ccttgctatt
     4261 ttattcgatc ataccagcga gagctacaat gttggggact tgtttatcta gctgcgtctg
     4321 attaaccttt attatttttc ctgaaacagt tttatttcct gcagttaatg tcgcgattgg
     4381 aatacctaag gctccagata cgacagatgc tgtgtttgca tcatttactt catactgaaa
     4441 catattcctg aaagatcttt tattatttgg cgt
//
