LOCUS       NZ_CP102700             2101 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Escherichia coli strain GN4554 plasmid pGN4554-6, complete sequence.
ACCESSION   NZ_CP102700
VERSION     NZ_CP102700.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30273450
            Assembly: GCF_029201525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Seah,C. and Melano,R.G.
  TITLE     Detection and prevalence of carbapenemase-producing uropathogenic
            Enterobacteriaceae isolated from patients in a tertiary care
            hospital in Jamaica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Seah,C. and Melano,R.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029201525.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 05:25:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,889
            CDSs (total)                      :: 4,764
            Genes (coding)                    :: 4,525
            CDSs (with protein)               :: 4,525
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 71 of 239
            Pseudo Genes (incomplete)         :: 153 of 239
            Pseudo Genes (internal stop)      :: 46 of 239
            Pseudo Genes (multiple problems)  :: 31 of 239
            Pseudo Genes (short protein)      :: 4 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102700.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN4554"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pGN4554-6"
                     /geo_loc_name="Jamaica"
                     /lat_lon="18.1096 N 77.2975 W"
                     /collection_date="2021-06"
                     /collected_by="Camille-Ann Thoms-Rodriguez"
     gene            complement(join(2069..2101,1..234))
                     /locus_tag="NV952_RS24375"
                     /old_locus_tag="NV952_24310"
     CDS             complement(join(2069..2101,1..234))
                     /locus_tag="NV952_RS24375"
                     /old_locus_tag="NV952_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(238..1203)
                     /locus_tag="NV952_RS24380"
                     /old_locus_tag="NV952_24315"
     CDS             complement(238..1203)
                     /locus_tag="NV952_RS24380"
                     /old_locus_tag="NV952_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(579..810)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 accgtttaaa tctattttca caaaaagagt ttttaccctc tcgtttgttt tctgaatata
       61 taaaattaat tcatcctcaa aaccttcgtg cggatgaaaa ccaaatgtgc caaaacttaa
      121 tccatcaata agcaaaataa ggtttttaaa cctgtcagga atcaccgctt cacttcttaa
      181 aaaaaataaa ccaatagtct tatttccatt caggtattca atattaatat tcatacgtta
      241 atccgattta tccttctcag gggcgcgttt gtaacgccgt ttacctgaat cccagacaaa
      301 cgccgtccgc ttcccgtcat cagtgccatc ccctacatcg tcggcaatga caagatcctc
      361 attggtttct cgatccaact ggaggacgtt ttttagcgcc ccaccggtcg agataaaacg
      421 gcgcttgtga agctgccgcg tcagctcaag aaaccactca ggatcgtttg ccatatcttc
      481 cggtttaacg gagtatttca gcgtttcagc aaccgcgctt tgcagttgct cggcaacgtt
      541 ggccacaacc tcacctgtct tagtttttac tgcccgaata tcgatattcg gctcatagtt
      601 cacccgcaag caatcgcgcc agagttctac ccaacgttcg tgcttaacgt agttcttccc
      661 tttaaaccaa gaaggttgca ccatcagcag acagtgaaaa tgcggatgtg cgctgccatc
      721 cttacctcgc gtcacctccg tagccctgat ccacccctga acaggtgata gctcctttcg
      781 cttttccatt cgcttaaacg ccgcattcat tgctgtaagg actgttccaa gttcacctat
      841 ctcgcagttc ctgacagtta acgtcagaaa caaccatcgg gaagacgggt aatccacaac
      901 gattttcggt agagcctgat aaaaacgggc ttgccacatg agggttcttc tccactggca
      961 gacagggcaa tgacggacac ggcaaaattg cgcactacga agccgtaggc gcgtttctcc
     1021 ggtttccatg atggtagacc aaccaaaacg taacaaaccg ctacaggagg ccatacggag
     1081 tgcatagcgc tcgaattcag cagcagtctg gtagataccc ccgacagaat cggtaagcgt
     1141 gcgctgggta tcccaaactg catcacgggg gctgtagtcc gaaaggaatt tatcttctga
     1201 cattaagcct gctccgatct gggcaggctg tgatttgatg tggtatattc atcacgtacc
     1261 tgtgcattgc gagccagtac atgtcaccag agtttgccgc tgaggtgaca cattcgaaat
     1321 gttggggagt gttgacgcac tccccaacgc cgattaaaaa cgcctcaatt ctgctccatt
     1381 ttctttgacc tgtcaaaacc cactcaaagc ctcgtcatta ctgggctaaa ccactatttc
     1441 atatccgcca tttatccctt aagtataaat acaaggatca cgcgcgagaa ttgcgcaaaa
     1501 aataaccaca attttaaccc cccaaaacaa gcacccccga acggcttacc ccaacctttt
     1561 ccacacaccc gatcaccaaa acagggaata aaaacaaaac cctgtcttac aggtggatgg
     1621 cctcacttcg tgaaaccatc caccccgcat aagtttttta atcggtggtg tcgtcaatca
     1681 ctaaatcgga accccaaagg ggacccccac gatttagcat ttgacgagat taactctcgg
     1741 agatggcatc atgcttttaa cggactttaa aaaacggtga acggatgcgg attttaatcc
     1801 gtgtctgtga ggcgttgagc aacgagcaaa gcgaggcggt agaaagagag ccagcggcag
     1861 gctcaggacg ataatccggg gaaaatgtac cactcgcaga gtgttaacat ttgacatcgg
     1921 attgtctatc ctgcattggt gtcgagttgg caaggctgag gataatgctg aggataatgc
     1981 tgaggataat gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc
     2041 tcagcctgcc gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga
     2101 t
//
