LOCUS       NZ_CP102699            10723 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Escherichia coli strain GN4554 plasmid pGN4554-5, complete sequence.
ACCESSION   NZ_CP102699
VERSION     NZ_CP102699.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30273450
            Assembly: GCF_029201525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Seah,C. and Melano,R.G.
  TITLE     Detection and prevalence of carbapenemase-producing uropathogenic
            Enterobacteriaceae isolated from patients in a tertiary care
            hospital in Jamaica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10723)
  AUTHORS   Seah,C. and Melano,R.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029201525.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 05:25:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,889
            CDSs (total)                      :: 4,764
            Genes (coding)                    :: 4,525
            CDSs (with protein)               :: 4,525
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 71 of 239
            Pseudo Genes (incomplete)         :: 153 of 239
            Pseudo Genes (internal stop)      :: 46 of 239
            Pseudo Genes (multiple problems)  :: 31 of 239
            Pseudo Genes (short protein)      :: 4 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102699.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN4554"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pGN4554-5"
                     /geo_loc_name="Jamaica"
                     /lat_lon="18.1096 N 77.2975 W"
                     /collection_date="2021-06"
                     /collected_by="Camille-Ann Thoms-Rodriguez"
     gene            join(10395..10723,1..910)
                     /gene="mrx(A)"
                     /locus_tag="NV952_RS24305"
                     /old_locus_tag="NV952_24240"
                     /gene_synonym="mrx"
     CDS             join(10395..10723,1..910)
                     /gene="mrx(A)"
                     /locus_tag="NV952_RS24305"
                     /old_locus_tag="NV952_24240"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="WP_000004159.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWAG
                     ATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTGLI
                     ACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGAAIG
                     LLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLLRPLF
                     GIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVGARISA
                     QNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRTPPELLG
                     RVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQFATMAAA
                     ATASAPTHTEGEH"
                     /name="mrx(A)"
     gene            910..1494
                     /gene="mphR(A)"
                     /locus_tag="NV952_RS24310"
                     /old_locus_tag="NV952_24245"
     CDS             910..1494
                     /gene="mphR(A)"
                     /locus_tag="NV952_RS24310"
                     /old_locus_tag="NV952_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="WP_001137892.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
                     /name="mphR(A)"
     gene            complement(1987..2751)
                     /locus_tag="NV952_RS24315"
                     /old_locus_tag="NV952_24250"
     CDS             complement(1987..2751)
                     /locus_tag="NV952_RS24315"
                     /old_locus_tag="NV952_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            complement(2869..>2934)
                     /locus_tag="NV952_RS24320"
                     /old_locus_tag="NV952_24255"
                     /pseudo=""
     CDS             complement(2869..>2934)
                     /locus_tag="NV952_RS24320"
                     /old_locus_tag="NV952_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /name="EAL domain-containing protein"
     gene            complement(2978..3283)
                     /locus_tag="NV952_RS24325"
                     /old_locus_tag="NV952_24260"
     CDS             complement(2978..3283)
                     /locus_tag="NV952_RS24325"
                     /old_locus_tag="NV952_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_000130000.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTV
                     YPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
                     /name="PadR family transcriptional regulator"
     gene            complement(3294..4499)
                     /gene="chrA"
                     /locus_tag="NV952_RS24330"
                     /old_locus_tag="NV952_24265"
     CDS             complement(3294..4499)
                     /gene="chrA"
                     /locus_tag="NV952_RS24330"
                     /old_locus_tag="NV952_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /GO_function="GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="WP_000184001.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRRR
                     WLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILFAL
                     GISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLVPSA
                     WGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVLAELM
                     PSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAVPGPLF
                     TFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQAALAGIN
                     AAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGAAGWLLSV
                     AL"
                     /name="chrA"
     gene            complement(4655..4858)
                     /locus_tag="NV952_RS24335"
                     /old_locus_tag="NV952_24270"
     CDS             complement(4655..4858)
                     /locus_tag="NV952_RS24335"
                     /old_locus_tag="NV952_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000376616.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARID
                     RTFAETFVLRSELLGIASENGK"
                     /name="hypothetical protein"
     gene            complement(4986..5825)
                     /gene="sul1"
                     /locus_tag="NV952_RS24340"
                     /old_locus_tag="NV952_24275"
     ARG             complement(4990..5825)
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5819..6166)
                     /locus_tag="NV952_RS24345"
                     /old_locus_tag="NV952_24280"
     CDS             complement(5819..6166)
                     /locus_tag="NV952_RS24345"
                     /old_locus_tag="NV952_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="WP_000679427.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacE delta 1"
     gene            complement(6372..7160)
                     /locus_tag="NV952_RS24350"
                     /old_locus_tag="NV952_24285"
     CDS             complement(6372..7160)
                     /locus_tag="NV952_RS24350"
                     /old_locus_tag="NV952_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="WP_000503573.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKPD
                     SDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYPAR
                     RELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKEHFS
                     KALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPAEHRP
                     LICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
                     /name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
     gene            complement(7291..7764)
                     /gene="dfrA17"
                     /locus_tag="NV952_RS24355"
                     /old_locus_tag="NV952_24290"
     ARG             complement(7295..7764)
                     /name="dfrA17"
                     /gene="dfrA17"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7922..>8602
                     /gene="intI1"
                     /locus_tag="NV952_RS24360"
                     /old_locus_tag="NV952_24295"
                     /pseudo=""
     CDS             7922..>8602
                     /gene="intI1"
                     /locus_tag="NV952_RS24360"
                     /old_locus_tag="NV952_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /name="intI1"
     gene            8667..9371
                     /locus_tag="NV952_RS24365"
                     /old_locus_tag="NV952_24300"
     CDS             8667..9371
                     /locus_tag="NV952_RS24365"
                     /old_locus_tag="NV952_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            9493..10398
                     /locus_tag="NV952_RS24370"
                     /old_locus_tag="NV952_24305"
     CDS             9493..10398
                     /locus_tag="NV952_RS24370"
                     /old_locus_tag="NV952_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000219391.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDD
                     GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMV
                     IQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDV
                     DRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSE
                     ARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA
                     LDSGNEEYLAAAKAQLAAAE"
                     /name="Mph(A) family macrolide 2'-phosphotransferase"
     ARG             complement(5823..6166)
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9494..10395
                     /name="mph(A)"
                     /gene="mph(A)"
                     /gene_name="Mph(A) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6376..7160)
                     /name="aadA5"
                     /gene="aadA5"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9494..10398
                     /name="mphA"
                     /gene="mphA"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             10396..10721
                     /name="Mrx"
                     /gene="Mrx"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="26.46"
     ARG             3..910
                     /name="Mrx"
                     /gene="Mrx"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="73.3"
ORIGIN
        1 tgccgcgttc gacgcacccg gtatggccgc gcaggacagt gagctgccca aactcggcca
       61 cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa gcggtgatcg ggaacgtcgc
      121 gattctaggt ggcccggccc ttgggggggc cgcaatcggc ctgcttggcg ctgcgccaac
      181 gctcgggctg acggcgttct gctccgtcct tgcaggtctg ctcggcgcgt gggtgcttcc
      241 cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc tccatgcgcg ccggcgtcgc
      301 ttttctctgg agcgaacccc tgctgcgccc tctctttggt atagtgatga tcttcgtggg
      361 catcgttggc gccaacggca gcgtcatcat gcctgcgctg tttgtagatg caggacgcca
      421 agtagcagag ctcgggctgt tctcctcaat gatgggggct ggtggtctcc ttggcattgc
      481 cattcatgcg tcggtcggcg cccggatatc agcgcagaac tggctggcgg tggcattttg
      541 tggctctgcg gtgggctcgc ttctgctttc acagttgcca ggcgtgccgg tgctgatgtt
      601 gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct cccattctca acgctgccat
      661 ctacaaccgc acgccgccag aacttctcgg ccgggtactc ggcacggtct cggcggtgat
      721 gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg tttgtcgacc ttgctggtcc
      781 gctccctggc ctcgttgtat cggccgtgtt tgcggggctc gtggctctac tctcgctccg
      841 tcttcaattt gctacaatgg cggcggcagc cacagcctcc gccccaaccc atacagaagg
      901 tgaacactga tgccccgccc caagctcaag tccgatgacg aggtactcga ggccgccacc
      961 gtagtgctga agcgttgcgg tcccatagag ttcacgctca gcggagtagc aaaggaggtg
     1021 gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc gcgatacgct gctggtgagg
     1081 atgatggagc gcggcgtcga gcaggtgcgg cattacctga atgcgatacc gataggcgca
     1141 gggccgcaag ggctctggga atttttgcag gtgctcgttc ggagcatgaa cactcgcaac
     1201 gacttctcgg tgaactatct catctcctgg tacgagctcc aggtgccgga gctacgcacg
     1261 cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc gcaagcgact gcccccaggt
     1321 gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg ctggcgcgac gatgcagtgg
     1381 gccgtcgatc cggatggtga gctagctgat catgtgctgg ctcagatcgc tgccatcctg
     1441 tgtttaatgt ttcccgaaca cgacgatttc caactcctcc aggcacatgc gtaaacggag
     1501 gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc gtcgattagt accggtacgg
     1561 tcggtggtat cgaagtcttg atcaccactc aggtctacgg cttacaaatg gtgaccatcc
     1621 cgatacttgc gtcagagcac cgggccgatt ctttgacagt gaatcactcc cgtaaggttg
     1681 tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca atgcgcccag caaacaacct
     1741 ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt ggacaagttg gtgtagcagc
     1801 acgagcacgg cgagataaca tcgcaggagt tcgacatgct caagagacag ctgattgcga
     1861 atcgcgatgc agattcataa cccgattgcg ggttggcttc actccaccat caccgagcag
     1921 actagcacgg cgggctctgt tgcaaagatt ggcggcagtc agaggtaggc tgtcgctctg
     1981 cgccgatcag gcggctgctg cgaaatggtg gttgagcatg cccatggcct ccgtcagcgc
     2041 cgagggccca atgccaaaag ctctctccac aaggcgcacc tcgcccctga tgccgggctg
     2101 caggcaccag gggcgagcct gtcctttgcg cagggctcgc atgacttcga atcccttgat
     2161 cgtggcatag gccgtgggga tcgatttgaa accgcgcacc ggcttgatca gtatcttgag
     2221 ctttccgtga tcggcctcga tcacgttatt gagatacttc acctgccggt gggccgtctc
     2281 ccggtccagc tttccttcgc gcttcaattc ggtgatcgct gcaccatagc tcggcgcttt
     2341 gtcggtattg agcgtggcag gcttttccca gtgcttcagg cctcgcaggg ccttgcccag
     2401 gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg tagaaatcga tcgtgtcgcc
     2461 ccgcttgtcg actgcccggt acaggtaggt ccacttgccc cgcaccttga cgtaggtttc
     2521 atccaggcgc cagctcggat caaagccacg ccgccagaac cagcgcagcc gcttctccat
     2581 ctccggggcg tagcactgga cccagcgata gatcgtcgta tggtcgaccg aaatgccgcg
     2641 ttccgccagc atttcctcaa ggtcgcgata gctgatcgga tagcgacaat accagcgcac
     2701 cgcccacagg atcacatcac cctggaaatg gcgccacttg aaatccgtca tcgttccgtc
     2761 cgtccaatct ccgccaagca tgctcaagct tcacgatttt tgcaacagag cccacacgag
     2821 tattgagcat agtcgagatt ggtgcagatc acttctgata ttgaactgtc aggagctggc
     2881 tgcacaacag ccattacgcc caatcaactg gtgcagtcgt cttctgaaaa tgacaatcca
     2941 gttagggtat agctcaacct gacatagaag caaaaactca accaccttct accaactctc
     3001 cgaacagctc cttgaccttt gttttcgcat cagcaagtgc agttctgcct tgttcagtga
     3061 tgtcataaac acgccgttca cgtcgcccgg tgcgttcgtg gcgtgaggtc agatagcctt
     3121 ttttttccag gccgtgcagc atcgggtaca cggtgccagc gctcatctcg tagccgtgtc
     3181 ggcgtagctc ttcgatgatc cccagcccaa agacaggttc ctcggctgca tggtgaagga
     3241 tgtgcaggcg gatcaaaccg ccgtagaggt ctttgtcagt cattttttgt gcctcacaga
     3301 gcgacgctca acagccaccc agctgcaccg ctaccgagga caaccagcca cggcgggagc
     3361 ttccagaaca taagtgcgac aagggcaact aatgccaagc cgaagtcttg cggctgaaag
     3421 atggcgctag tccatacagg ctgatacagc gcggccagca gcaagccgac tacagcggca
     3481 ttgatcccgg ccagcgcagc ttggatgcct gtattgcggc gcaaacgctc ccaaaatggc
     3541 attgatccga cgaccagcaa gaacgagggc gcgaagatag ccagcagaca cacaatgccg
     3601 ccgatccagc ccgacggggc ggtgttcatc gaggcaccaa gaaacgcggc gaacgtgaac
     3661 aaagggccgg gcaccgcttg agctgccccg taccccgcga gaaaggattc attgttgacc
     3721 cagccggagg gcaccacttc ggcttgcagt aatggcagca caacgtgacc accgccgaac
     3781 accagtgatc cgacacgata gaaggaatcc accattgcca tggtttgact tggcatcagt
     3841 tcggccaaca ccggcaggcc aatcagcaag acaaagaaca gcgagagcca aagcacgccg
     3901 gcccggtgac tgaccgtgat aggtaggggg tcatgctcaa caactttcgc tggcttgaac
     3961 aataaccggc ctgcgatgcc tgcgatagca atcacgccaa cctgtcccca cgcggacggc
     4021 acaagtaaaa cgacgcaggt agcaattgcc atgatggtga ctcgcagccc atccgtgcat
     4081 aggttacgcg ccatgcccca tactgcttga gcgaccacgg ccacagccac cacttttaag
     4141 ccatgcaacg cgccctgcga gacgtaatcg ccatagctgg agatgccgag cgcaaaaagg
     4201 atcaaggcta tggcagacgg cagcgtgaag ccagcccaag cagccagcgc cccgctgtat
     4261 ccagcccgag acagtcctac cgctatgccg acctggctgc ttgcaggccc tggcaagaac
     4321 tgacaaagcg cgaccaagtc agcatagctc cgttcggaga gccagcgccg ccgtgtgaca
     4381 aattcggcgc ggaagtagcc caagtgcgca atggggccgc caaaagatgt caatccaagc
     4441 cgcagaaaaa taagaaagac cgaccatggt ctgctgtcat cggtagggtt attcgtcata
     4501 ctttcgcctt catgatctgc aacgagttga tcaataataa gcgaaattcg ataacgaaat
     4561 tcgatataaa tctagaaaaa aatacctcta tgtgtactac gcagttttag ctgtggcttt
     4621 cacaggagca cgcttactta cggcttagcg tgctttattt tccgttttct gaggcgatcc
     4681 ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt ccggtcgatc cgcgcgacgt
     4741 cccaggcggg cgttcccttg gcggacatcc acgccgcagc gtcgtgcatc agccgcacaa
     4801 cctcgtcgat atcacccgag caggcgaccc gaacgttcgg aggctcctcg ctgtccattc
     4861 gctcccctgg cgcggtatga accgccgcct catagtgcag tttgatcctg acgagcccag
     4921 catgtctgcg cccaccttcg cggaacctga ccagggtccg ctagcgggcg gccggaaggt
     4981 gaatgctagg catgatctaa ccctcggtct ctggcgtcgc gactgcgaaa tttcgcgagg
     5041 gtttccgaga aggtgattgc gcttcgcaga tctccaggcg cgtgggtgcg gacgtagtca
     5101 gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg ctggacccag atcctttaca
     5161 ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca ccgagaccaa tagcggaagc
     5221 cccaacgccg acttcagctt ttgaaggttc gacagcacgt gcagcgatgt ttccggtgcg
     5281 gggctcaaga aaaatcccat ccccggatcg aggatgagcc ggtcggcagc gaccccgctc
     5341 cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa tctcgtcgag cgcgtcttcg
     5401 ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct gcgctgagtg cataaccacc
     5461 agcctgcagt ccgcctcagc aatatcggga tagagcgcag ggtcaggaaa tccttggata
     5521 tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct gggtttccgg ttggaagctg
     5581 tcgattgaaa cacggtgcat ctgatcggac agggcgtcta agagcggcgc aatacgtctg
     5641 atctcatcgg ccggcgatac aggcctcgcg tccggatggc tggcggccgg tccgacatcc
     5701 acgacgtctg atccgactcg cagcatttcg atcgccgcgg tgacagcgcc ggcggggtct
     5761 agccgccggc tctcatcgaa gaaggagtcc tcggtgagat tcagaatgcc gaacaccgtc
     5821 accatggcgt cggcctccgc agcgacttcc acgatgggga tcgggcgagc aaaaaggcag
     5881 caattatgag ccccatacct acaaagcccc acgcatcaag cttttgccca tgaagcaacc
     5941 aggcaatggc tgtaattatg acgacgccga gtcccgacca gactgcataa gcaacaccga
     6001 cagggatgga tttcagaacc agagaaagaa aataaaatgc gatgccataa ccgattatga
     6061 caacggcgga aggggcaagc ttagtaaagc cctcgctaga ttttaatgcg gatgttgcga
     6121 ttacttcgcc aactattgcg ataacaagaa aaagccagcc tttcatgata tatctcccaa
     6181 tttgtgtagg gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt
     6241 tggctgtgag caattatgtg cttagtgcat ctaacgcata gttgagcggc gggcgcagcc
     6301 cgtccgcttg aacgccgagt taggcatcag atgccctcgg cgcgggtcga tgcacttttc
     6361 gcacatgccg ctcaacgcaa gattctctca atcgttgctt tggcatatcg aacgaacgcg
     6421 gccgtctctt cgacgcgcat tgctaggtcg tcgtcctcgc tacccaggta cgccgcgcgt
     6481 gccttgcaga tgaggggccg atgctcggca ggcaaacgct ccgataccca tgcggcagca
     6541 acgtccttag gagcaatgag accagttgaa gcgctgtacc aaatgcgagc aagagcaaga
     6601 acgacgttcc gctcgtcacc cttccaatcc gactctgcat tccactgggc aatagtgtcg
     6661 aaaagcgcct tggagaaatg ctccttcggc accggctcga aaaacgtggc tgcggatggg
     6721 cctagaagcg caaggctgtg ttgcctcgcc ttggtcagca aaatcgcaag atcgtgatcc
     6781 agaacggcag gctcgaacgt tccggaaagg atgtcgtggc ggagccactc accgaactga
     6841 agctcacgcc gcgccggata gcgccaaggc actacttcgc ttcgagcgac aacagttagc
     6901 tccagcggtc gccatgttcc gccatcgcct ggcggtgatg agactttcag caaatcgagc
     6961 attagcgcct gccggagcga atcgttaggt gcggcgctga cggtcacgag caagtctatg
     7021 tcgctgtccg gcttcagccc tccatcgatc gcagatccga acaggtggat tgtgtccagt
     7081 gtcgcagcca gatggcgctc gatcaccgcg cgagcgtggg acagctgctt gaaaacttgt
     7141 gcagggaaaa attcacccat gatgcctaac gttaagttca gcggcagctt ttaagttgcg
     7201 gctttgtgga atacttttgc gcagcaaaac cacaaagacg cgacttaaaa gctgtccaag
     7261 gagcgaagcg actggtgctg caacgcattg ttagcctttt ttccaaatct ggtatgtata
     7321 atttatatta gacataaaaa actgttcaaa aaccaaattg aaattctcag gcattatagg
     7381 gaatttgata tcaccttcga cttcaacgtg aacagtagac aaatgaatta tatctgcttt
     7441 ttcaataagg ctattataga tttgaccccc gccagagaca tatacatgat ctgtaacttt
     7501 tgatagctct ttcaaagcat tttctattga aggaaaaact aggacgtttt catttgagct
     7561 tgaaattccg ttctttgaca ctactgcata tttgcgattt ggaagaacac ccatagagtc
     7621 aaatgttttt cttccgacaa ggagccattg attatatgtg agcgctttaa agagtagttg
     7681 ctcacctttt actgaccacg ggatatcagg accactaccg attacgccat tttctgacac
     7741 tgcagaaatc aatgatattt tcaatttaac tcccttaatg gctaactttg ttttagggcg
     7801 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     7861 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
     7921 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     7981 gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt atgtccactg
     8041 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     8101 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
     8161 tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg
     8221 gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga
     8281 tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccagcttct
     8341 gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg atctggattt
     8401 cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg ccttgatgtt
     8461 acccgagagc ttggcaccca gcctgcgcga gcagctgtcg cgtgcacggg catggtggct
     8521 gaaggaccag gccgagggcc gcagcggcgt tgcgcttccc gacgcccttg agcggaagta
     8581 tccgcgcgcc gggcattcct ggcggcactg ttgcaaatag tcggtggtga taaacttatc
     8641 atcccctttt gctgatggag ctgcacatga acccattcaa aggccggcat tttcagcgtg
     8701 acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac cgtgagctgc
     8761 aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac cgctgggttc
     8821 agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac ccttccgatc
     8881 tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg gcgtatctgt
     8941 accgggccgt cgacagccgg ggccgcactg tcgattttta tctctcctcc cgtcgtaaca
     9001 gcaaagctgc ataccggttt ctgggtaaaa tcctcaacaa cgtgaagaag tggcagatcc
     9061 cgcgattcat caacacggat aaagcgcccg cctatggtcg cgcgcttgct ctgctcaaac
     9121 gcgaaggccg gtgcccgtct gacgttgaac accgacagat taagtaccgg aacaacgtga
     9181 ttgaatgcga tcatggcaaa ctgaaacgga taatcggcgc cacgctggga tttaaatcca
     9241 tgaagacggc ttacgccacc atcaaaggta ttgaggtgat gcgtgcacta cgcaaaggcc
     9301 aggcctcagc attttattat ggtgatcccc tgggcgaaat gcgcctggta agcagagttt
     9361 ttgaaatgta aggcctttga ataagacaaa aggctgcctc atcgctaact ttgcaacagt
     9421 gccggattga atataaccga cgtgactgtt acatttaggt ggctaaaccc gtcaagccct
     9481 caggagtgaa tcatgaccgt agtcacgacc gccgatacct cccaactgta cgcacttgca
     9541 gcccgacatg ggctcaagct ccatggcccg ctgactgtca atgagcttgg gctcgactat
     9601 aggatcgtga tcgccaccgt cgacgatgga cgtcggtggg tgctgcgcat cccgcgccga
     9661 gccgaggtaa gcgcgaaggt cgaaccagag gcgcgggtgc tggcaatgct caagaatcgc
     9721 ctgccgttcg cggtgccgga ctggcgcgtg gccaacgccg agctcgttgc ctatcccatg
     9781 ctcgaagact cgactgcgat ggtcatccag cctggttcgt ccacgcccga ctgggtcgtg
     9841 ccgcaggact cggaggtctt cgcggagagc ttcgcgaccg cgctcgccgc cctgcatgcc
     9901 gtccccattt ccgccgccgt ggatgcgggg atgctcatcc gtacaccgac gcaggcccgt
     9961 cagaaggtgg ccgacgacgt tgaccgcgtc cgacgcgagt tcgtggtgaa cgacaagcgc
    10021 ctccaccggt ggcagcgctg gctcgacgac gattcgtcgt ggccagattt ctccgtggtg
    10081 gtgcatggcg atctctacgt gggccatgtg ctcatcgaca acacggagcg cgtcagcggg
    10141 atgatcgact ggagcgaggc ccgcgttgat gaccctgcca tcgacatggc cgcgcacctt
    10201 atggtctttg gtgaagaggg gctcgcgaag ctcctcctca cgtatgaagc ggccggtggc
    10261 cgggtgtggc cgcggctcgc ccaccacatc gcggagcgcc ttgcgttcgg ggcggtcacc
    10321 tacgcactct tcgccctcga ctcgggtaac gaagagtacc tcgctgcggc gaaggcgcag
    10381 ctcgccgcag cggaatgagc gaacgtcgat atagcccgct cgcgacgctg ttcgcggcga
    10441 cctttctctt ccggatcggc aacgcggtgg cggccctcgc gcttccatgg ttcgtcctgt
    10501 ctcatacaaa gagcgcggcc tgggcgggcg ccacggccgc tagcagcgtc atcgcgacca
    10561 tcatcggcgc gtgggttggt ggtggcctcg tcgatcggtt cgggcgcgcg cccgtcgcat
    10621 tgatctcggg tgtggtgggc ggcgtggcca tggcgagcat cccactgctc gatgccgttg
    10681 gcgccctctc gaacactggg ctgatcgctt gcgtggtgct cgg
//
