LOCUS       NZ_CP102691             9294 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain GN4539 plasmid pGN4539-4, complete
            sequence.
ACCESSION   NZ_CP102691
VERSION     NZ_CP102691.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30273448
            Assembly: GCF_029201485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Seah,C. and Melano,R.G.
  TITLE     Detection and prevalence of carbapenemase-producing uropathogenic
            Enterobacteriaceae isolated from patients in a tertiary care
            hospital in Jamaica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Seah,C. and Melano,R.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029201485.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 05:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,346
            CDSs (with protein)               :: 5,346
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 53 of 136
            Pseudo Genes (incomplete)         :: 80 of 136
            Pseudo Genes (internal stop)      :: 19 of 136
            Pseudo Genes (multiple problems)  :: 15 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102691.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GN4539"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pGN4539-4"
                     /geo_loc_name="Jamaica"
                     /lat_lon="18.1096 N 77.2975 W"
                     /collection_date="2021-06"
                     /collected_by="Camille-Ann Thoms-Rodriguez"
     gene            complement(join(9094..9294,1..99))
                     /locus_tag="NV950_RS27960"
                     /old_locus_tag="NV950_27945"
     CDS             complement(join(9094..9294,1..99))
                     /locus_tag="NV950_RS27960"
                     /old_locus_tag="NV950_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(89..352)
                     /locus_tag="NV950_RS27965"
                     /old_locus_tag="NV950_27950"
     CDS             complement(89..352)
                     /locus_tag="NV950_RS27965"
                     /old_locus_tag="NV950_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(658..1077)
                     /locus_tag="NV950_RS27970"
                     /old_locus_tag="NV950_27955"
     CDS             complement(658..1077)
                     /locus_tag="NV950_RS27970"
                     /old_locus_tag="NV950_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1480..3165
                     /locus_tag="NV950_RS27975"
                     /old_locus_tag="NV950_27960"
     CDS             1480..3165
                     /locus_tag="NV950_RS27975"
                     /old_locus_tag="NV950_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3175..3432
                     /locus_tag="NV950_RS27980"
                     /old_locus_tag="NV950_27965"
     CDS             3175..3432
                     /locus_tag="NV950_RS27980"
                     /old_locus_tag="NV950_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            3517..3666
                     /locus_tag="NV950_RS27985"
                     /old_locus_tag="NV950_27970"
     CDS             3517..3666
                     /locus_tag="NV950_RS27985"
                     /old_locus_tag="NV950_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5147..7111
                     /locus_tag="NV950_RS27990"
                     /old_locus_tag="NV950_27975"
     CDS             5147..7111
                     /locus_tag="NV950_RS27990"
                     /old_locus_tag="NV950_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            7111..7842
                     /locus_tag="NV950_RS27995"
                     /old_locus_tag="NV950_27980"
     CDS             7111..7842
                     /locus_tag="NV950_RS27995"
                     /old_locus_tag="NV950_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            7849..8379
                     /locus_tag="NV950_RS28000"
                     /old_locus_tag="NV950_27985"
     CDS             7849..8379
                     /locus_tag="NV950_RS28000"
                     /old_locus_tag="NV950_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8407..8586)
                     /locus_tag="NV950_RS28005"
                     /old_locus_tag="NV950_27990"
     CDS             complement(8407..8586)
                     /locus_tag="NV950_RS28005"
                     /old_locus_tag="NV950_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8655..9032
                     /locus_tag="NV950_RS28010"
                     /old_locus_tag="NV950_27995"
     CDS             8655..9032
                     /locus_tag="NV950_RS28010"
                     /old_locus_tag="NV950_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     BGC             1..8165
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3770..4348)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7112..7839
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gttctgcgca tcccacaggg atgaattttc cgcgatatag tcaaacagcg tcgttacatc
       61 ctcgatggct ccctgcttcc attccacgtc atacggcatc gcgcagttcc tgataacggc
      121 catgcaggcg ctggcggagt tgttccgtgg tcatacccgg acgcgtgtca gcacgggcgg
      181 cggtcacttt ggcgcgaacc tgatcttcat actctgcctg ggccagttct tcgttagcca
      241 gtcgctgcaa cagcgcagct ttgtccgggc caccttccgg cagtatgcgg ggcaggcttt
      301 cagccagttc ccgaagaaag ttggcgtttt cgtagtgtgt agtgttgctc attgcggagc
      361 ctcccgggtt aatgcggcaa tatgaaggaa gtttagcagg gggcggtctg aaagaatatg
      421 gtcgtgtcag tcaggctgat gaagcagaaa gtcggtaaaa aagggcttac ggcatccatt
      481 ttacgtcaaa acatatgcca tgcagttgcg tgcataggca tgcattagga taaaatttat
      541 cgggcgcgtt tccggcagtt ttccgggggg gttgttgcct gttttatccc gtagccgccg
      601 gaaacgccct gagcccgtct gagcggtgcg cgtaatgacg cgttatggta aatccactta
      661 ctgtctgcct tcatagccat cgagataaac agctgaaatg gtgtcagcca gtagccttga
      721 ttttttcggt gtgaggtctt tgctgcggta atcggtaccc gcaaattgtg ttgtgccttg
      781 gagtgagttt aaaaattcct gactgtgaaa ctgctgaatg cgttgctgca catcactccg
      841 tgattttccg gtcgctttat cctttgcgcc catatcccag aactgccgga aaaaaggcac
      901 catcattcgt gttgtgtcag ctttctgggt ggaaaaattg ggatcaaaac cgtcatctga
      961 agcatatacg taatgccggg tatgctgttc cacgctgccc tttggagcac aggcagtgag
     1021 agttgctgtc gctgttatca tggcgataag acacagtttg ctgccggaac gtctcatgag
     1081 cgtctcctta cctttaattc ttttttctca ttgagttata tatccgcagg ctatcttatc
     1141 atcagtttct gaccgtgttc agtgaccgaa caacccgaac cagacccccc gtttcttttc
     1201 ctgtcccgga gggggctttc tggtatctca tataatgatt tcagtaaaac atactgaggc
     1261 cggagaacgg cgccgtttgt gctggttttt gttgcatctg gaggggattg cgcatggtgt
     1321 gagtggctgt ttttttgatc attttcacaa aaacgcaaat ttacagactt gacatgaaaa
     1381 acaaggcagc gtaattttta tactgtatat aaaaccagtg gttatatgta cagtatttta
     1441 ttttagttta ttgttttaaa agtcaaagag gaaaagtata tgagtggcgg agacggacga
     1501 ggtccgggta attcaggtct gggacacaat ggtggtcagg ccagtgggaa tgtgaacggt
     1561 acatcaggta aaggtggccc ttcatcaggt ggtggtacgg atccaaacag cgggccgggc
     1621 tggggtacga cgcatacacc taacggggat attcataact ataacccggg ggagtttggt
     1681 aacggcggga gtaaacccgg tggtaatggc ggtaacagcg gcaatcatag cgggagctct
     1741 ggtggcggac agtcttcggc caccgcgatg gccttcggtc tgcctgcctt ggccactccg
     1801 ggggctgaag gactggcttt atccgtttcc ggcgatgcgt tgtcggccgc tgttgctgat
     1861 gtgctggctg ccctgaaagg tccgtttaag tttggtctgt gggggattgc gatttacgga
     1921 gtgctgcctt ctgagatagc aaaagatgat ccgaaaatga tgtcaaaaat cgtgacgtca
     1981 ttgccggcag acacggtaac ggagacgccg gtcagctccc tgccgctgga ccaggcgacg
     2041 gtcagcgtca ctaaacgtgt ggcggatatt gtgaaggacg agcggcagca tattgcggtt
     2101 gtcaccggcc ggccaatgag tgttcctgtg gtggatgcga aaccgacaaa acgtccgggg
     2161 gtattcagtg tgtcgattcc gggtctcccg tctctgcagg tgagcgtacc taaaggtgtt
     2221 ccggccgcga aagccccgcc aaaaggcatt attgctgaaa aaggtgattc acgtccggct
     2281 ggttttacgg ccggtggtaa ctcccgtgaa gccgttattc gtttcccgaa agagagcgga
     2341 cagaaaccgg tctatgtgtc ggtgacggat gttcttactc cggcacaggt aaaacagcgt
     2401 ctggaggaag aaaagcgtcg ccagcaggca tgggacgctg ctcacccgga agaggggctg
     2461 aaaagagagt atgataaagc gaaagctgag ctggatgctg aagataaaaa tattgcgacc
     2521 ttaaacagtc gcattgcatc gacagagaag gcgatccccg gtgcaagggc tgctgttcag
     2581 gaagccgata aaaaggtaaa agaggcagag gcaaataaag atgattttgt gacttataac
     2641 ccgcctcatg aatatggctc cgggtggcag gatcaggttc gctatcttga taaggatatt
     2701 cagaatcaga atgagaaatt aaaagctgcc cagacatctt taaacgaaat gaatgaatcc
     2761 ttatccaggg ataaggctgc gctttccggg gcgatggaga gccggaaaca aaaggagaaa
     2821 aaagcgaagg atgcagaaaa taaattaaat gaggaaaaga aaaaacctcg taagggaact
     2881 aaagattacg gccatgatta ttttcctgat cccaagactg aagatattaa ggggttggga
     2941 gagttgaaag agggtaaacc taaaacccct aaacaaggtg gtggtggtaa gcgggcccga
     3001 tggtatggtg ataaaaagcg taaaatttat gaatgggatt cccagcacgg tgagcttgaa
     3061 gggtaccgcg ccagtgatgg cgaacacctc ggggcattcg atccaaaaac gggtaagcag
     3121 gttaaagggc cggatccaaa acgaaacatt aaaaaatatc tttaagaggt aaatatgggg
     3181 cttaaattac atattcattg gtttgataag aaaaccgaag agtttaaagg tggtgaatac
     3241 tcaaaagact tcggtgatga tggttctgtc attgaaagtc tggggatgcc tttaaaggat
     3301 aatattaata atggttggtt tgatgttgaa aaatcatggg tttcgatatt acagccacac
     3361 tttaaaaatg taatcgatat tagtaaattt gattactttg tatcctttgt ttaccgggat
     3421 ggtaactggt aagcaaaagt tatcaaggat gatttgaaat acagacttcc ggtgttcagg
     3481 gatgaatacc ggggttttta aatacaggga aggtatatga aaaaggtcaa ggttgttttt
     3541 ctttttatat tgattttttc aggctttctt ctggtcgcat gtcaggcaaa ctatatccgg
     3601 gatgttcagg gtggaacggt ggcaccatcg tcctcctctg aactgacggg gatcgcggtt
     3661 cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atcttttgcc
     3721 ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa
     3781 ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg
     3841 tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg
     3901 cgaactccca gttaccaatg gctgctgcca gtggcgtttt gcgtgctttt ccgggttgga
     3961 ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacggggg gttcttgctt
     4021 acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagagtg tgagctatga
     4081 gaaagcgcca cacttcccgc aagggagaaa ggcggaacag gtatccggga aacggcaggg
     4141 ttggaacagg agagcgcaag agggagcgat tcatcggaaa cggtgatgat ctttaagtcc
     4201 tgtcgggttt cgccactcct gactgattca tggttgagcc accggctccc acagatgcac
     4261 cgaaaaagcg tctgtttatg tgaactcagt caggagggcg gagcctatgg aaaaacgcca
     4321 cctgcgtggc cttgccgttt tgcctcacat gttagtcccc tgcttattca cggaatctgt
     4381 gggtaacttt gcatgtgtcc gcagcgcccg ccgcagtctc acggccggag cgtagcgacc
     4441 gagtgagcga ggaggcgcaa tttccccggc acctacaaga cggacaccgg acgcgctgat
     4501 gtggcatatt gtcccctgat taagtcttta tattccctga taataaatta ccaggatagg
     4561 tgaatcatat cggtttttcg ctgaacattt tacgttaacc tatctctgtt gcttttttct
     4621 ccttacggag aaatacaggt catccccgcg agggcgtggc tgacctcagg gggatatccc
     4681 ctgcttacgc tggctggacg ccaccgacgc acggtctctc cccctctttc tggctccgtc
     4741 cggctggacg ccggccacgc cagaaattca cccggccccc gtcaggctca cgcctgacca
     4801 cccacaaaaa tccacggtgc tggacgcacc tggcgaagca gcaaagctgc tcctgatttt
     4861 gcgggtggtc agccgctcgc tatggcgaac tctgacgggg gccggaacac cggaaaagac
     4921 cgcactgggg tgccgccttt tcctctcaca cgctggggcg tgctcgttgt tcctccgggg
     4981 ctgcgccttt tccgcatggg gcggaaagag gctggcctcc gtggtgaaag ctgaacgcaa
     5041 tcaccacgga cgtgaccgcc gccgccatgc cttggggcat gtccgcggct tgtgcagcgg
     5101 gggctgcaca tcacgccggc aaaccatcgt ctgaaggaga gagaacatgt ttaatacgga
     5161 ttcgcttgcc tggaagtggc acattccgga gctggcccgg cagatttatc cgtgggtcag
     5221 ctggctgtac caggagtatt acggtctggc cggggctttc ctagttatcg gcctgttttt
     5281 cggtctggct gaactttggg ccggacggat gcgttttgtg gggctgagtc ataaggagcg
     5341 acgggagtat gccgatcgct ataagcgcga taataaacgg ccagcgccat tttcggtgct
     5401 ggcctcgctg gattcgcccg ggcggatggt cagggcacgt cgacatgtct ggaactacgc
     5461 gtactggctg accggcgcgg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt
     5521 tatcgagagc tggggagaaa ccgtcctgcg tccggacctc tacgcaaacg cagattatta
     5581 cctgctgcag ccggctaacc tgttcgggat tgcctggtgc gcgggcgggc tggctgtcgg
     5641 gattgtgttg gcgcggctgc tggcgttcct gctgcttgac ggtcattttg cggctgctga
     5701 cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggacaggaga
     5761 gatgaccgac gtgcgccacc tgcatttcgg ggagccggta ccggttaatg cgctggccga
     5821 tttttcctcg gagcaggccc ggcaggagca ggcggtgttt ctgggtaagg atgagcaggg
     5881 caagccggtg ctggttccgc gtgatacctg gcgcaagacc aacattcaga tcctcggcct
     5941 gcccgggagc ggcaagagcg tgatggccac caacgcgctc attcgctgcg tacgcgattt
     6001 cggggacgcg gtggtgtact tcgaccccaa aggggacgca tgggcgccgc acgtcttccg
     6061 ggcgcactgt ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa
     6121 tctgttccgc gatctcgacc agtacgcgct gaaaaacctg ctgattgccg ggcttaacct
     6181 gggcgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct
     6241 gattgcggag cagtttccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc
     6301 gctgccggag gccctgaaaa aggacgtgaa ggggttgatc actaggctgg aaaacgtcgc
     6361 tgacctgagc gtcctgcaga cggacagtgg catcgatgtg gcggggatta tcaacggcgg
     6421 cggctgcctg tacgttatcg gctcgatgga cgacgaggcg gtgatccgtg tgcaaaaaat
     6481 gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggac gagttccggg agtggccaca
     6541 cgtcagcgtg atgctcgatg aaattaaata tctgctgtcg aagtatgtac tgaacgcact
     6601 ggggacgctg cgcagccggg actgcaatct gttgctcgcg caccagtcgc tgggcgattt
     6661 cgggcagtgc gggcaggatc tgcccgctga atttgtgaaa accacggtac tggataacac
     6721 gccgatccgc tggttttacc gggcggccag cctggagtcc gcgcagtggg cggcggggca
     6781 gaccggggaa atcagggtgg acgtggagcg ccgccgtgcc agccgggagg cggggaacgt
     6841 ggagcatatc agcggggaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa
     6901 caccctgcaa cacctgccgg acgggttcgc ggtcatgacc gggctgggtg tggcccggat
     6961 ggcgttcagc agtccgctgc gggttgagcg ccgggaaatc ccgttgcagc gtttcccggt
     7021 gctggcgaaa accgatcccc tggcggagtt ccggcccccg gccgacggcg gcgggccgaa
     7081 agcggatgat gattttgcgg ggatttactg atggcgctgg agctgtacaa cgtcagccac
     7141 gcgaaacgac aggcgcggaa cgcggagaaa acccggctga ccctgcgctg gttgcgggaa
     7201 gagctgtgtt ccacggcgga gctggttgcg cggcggctgg ggattgcggc ggtgcagccg
     7261 gtctaccgct tcctcgacag cctggtggcg aaagggctgc tggtccggac caaatatccg
     7321 gtggacggcc gtcaggtcag cgtgtggggg ctgacgccgc atggcgtggc gttctcgttc
     7381 gacgaggacg aaccgctggc ggatgttatt ccgtttcagc cgtcaagggt gtcggcagca
     7441 catctgccgc accggctggc cgtgcaatcc ctgcgccttg atatggaagc cagaggggcc
     7501 accggctggc gcattctgca ccgtctgagt cttaaaggca tgaaagtgcc ggacgccctg
     7561 gcggagctgg acggtaaaac ggtggcgttt gaggtggagc gcaccgtcaa gagccgtcgc
     7621 cggtatcagg aagtggtgtc cgggtatctg ttcaaccgca aagctaacgg catcgatgaa
     7681 atctggtaca tctgcccgga ccgggcgacg caggtcaggg tgcagcgggc aatcctgtcg
     7741 gtggatgaaa tcgtgaaccc acagaccggc gaggcccgca aaacggcaga gctggatcgt
     7801 gaacggttgt ttgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac
     7861 atggtattta ttgaagacgc ggaggaccgg aacggcgagc gctgcatcat gccgaccagc
     7921 gagcatctgg aagatatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt
     7981 gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc
     8041 aacagcgacg aaaaagccac gtactttttc actggtaaaa atccgcaatg ggaacggaac
     8101 aaacccacag gaatgaagct gtgtgattac ctctatcgca aggtggagcg gttcgccatc
     8161 gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat taacgcgatg
     8221 attgacgtgg tgagtgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag
     8281 ctgatgcggg aatataacag ccgctatttt aacgtgccgg tacctgaact gccggaggac
     8341 aaacctgccc gccggtggtg gccgttcggc aaaaaataaa tgccagcacc ggccatgccg
     8401 gtgctgttat tcctccccga gcttatcccg catcacctgc cagagtgttt ccgcatcctc
     8461 gtgtagccgc tcgcacatgt cggccggggt atccatatcc agctcattca gtttatcgag
     8521 caatagcagc gactgctcct taataaattt agccatattg agggctgttt tttcctgttt
     8581 tgtcatgatt ctttccttcc gggcatggtg tgcgcctgtc aggtatactg tctcaaaaat
     8641 agcggggtga gacaatggcg tgggtgacgg tgagacaggc gacggaactg acgggtaaag
     8701 cccgaagttc cctgtatcgg gatatggcga aaggccgcgt atcataccgt acggaagctg
     8761 atggcggccg ggtggtggat accagcgagc ttatccgggt gtacggtgag ttaagacagg
     8821 atgagactca cgagcgggac gagttgagac tgtcgggtga gactgaaaaa atggcctccg
     8881 acgcgttgat cgcggagata aaagcactca gggaggaggt ggccgggcta cggcaggaaa
     8941 tgcagtccat gagactgctg gaacacaaac cggagacagc agagaccccg gccggaacac
     9001 cgcgccggtg gtggccgtgg ggaaaacgct gacgcgctgt gcttgtccaa ccggcgacgg
     9061 ccacgcaatg aattgcgtcc ccgtcaccgg ttattagaga acaatatggg gattctggcg
     9121 ctggccgacc acggccagaa cccggacttt tctggtctgg tcatcaacat catagaggac
     9181 atgctgtccc atcagactga tccggcgaac gtcctcgcca tagcgtgagt ccacttcata
     9241 gagtcgcggg aagtcggcca gtttgtccgc cgcagcgaga acccggtcgg taac
//
