LOCUS       NZ_CP102582            10967 bp    DNA     circular CON 01-APR-2024
DEFINITION  Acinetobacter baumannii strain A9844 plasmid p2A9844, complete
            sequence.
ACCESSION   NZ_CP102582
VERSION     NZ_CP102582.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30174926
            Assembly: GCF_028753295.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Sezmis,A.L., Woods,L.C., Peleg,A.Y. and McDonald,M.J.
  TITLE     Horizontal Gene Transfer, Fitness Costs and Mobility Shape the
            Spread of Antibiotic Resistance Genes into Experimental Populations
            of Acinetobacter Baylyi
  JOURNAL   Mol Biol Evol 40 (3) (2023)
   PUBMED   36788632
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   McDonald,M.J. and Sezmis,A.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2022) Biological Sciences, Biology, Science Store,
            Room G52, 17 Rainforest Walk (Building 23), Clayton Campus, Monash
            University, Monash University, Melbourne, Victoria 3800, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-FEB-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 350.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028753295.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 06:29:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,941
            CDSs (total)                      :: 3,844
            Genes (coding)                    :: 3,784
            CDSs (with protein)               :: 3,784
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 22 of 60
            Pseudo Genes (incomplete)         :: 29 of 60
            Pseudo Genes (internal stop)      :: 19 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102582.1.
            Bacteria and source DNA available from Dr. Mike McDonald,
            Biological Sciences, Monash University, Clayton, Victoria,
            Australia.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="A9844"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p2A9844"
     gene            1..951
                     /gene="repM"
                     /locus_tag="NUX13_RS19690"
                     /old_locus_tag="NUX13_19695"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="NUX13_RS19690"
                     /old_locus_tag="NUX13_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208778.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="NUX13_RS19695"
                     /old_locus_tag="NUX13_19700"
     CDS             944..1519
                     /locus_tag="NUX13_RS19695"
                     /old_locus_tag="NUX13_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096619.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSRQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1539..1682
                     /locus_tag="NUX13_RS19700"
                     /old_locus_tag="NUX13_19705"
     CDS             1539..1682
                     /locus_tag="NUX13_RS19700"
                     /old_locus_tag="NUX13_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(1871..2389)
                     /locus_tag="NUX13_RS19705"
                     /old_locus_tag="NUX13_19710"
     CDS             complement(1871..2389)
                     /locus_tag="NUX13_RS19705"
                     /old_locus_tag="NUX13_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            2762..3049
                     /locus_tag="NUX13_RS19710"
                     /old_locus_tag="NUX13_19715"
     CDS             2762..3049
                     /locus_tag="NUX13_RS19710"
                     /old_locus_tag="NUX13_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3036..3350
                     /locus_tag="NUX13_RS19715"
                     /old_locus_tag="NUX13_19720"
     CDS             3036..3350
                     /locus_tag="NUX13_RS19715"
                     /old_locus_tag="NUX13_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3478..5889
                     /gene="znuD2"
                     /locus_tag="NUX13_RS19720"
                     /old_locus_tag="NUX13_19725"
     CDS             3478..5889
                     /gene="znuD2"
                     /locus_tag="NUX13_RS19720"
                     /old_locus_tag="NUX13_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6194..6655
                     /locus_tag="NUX13_RS19725"
                     /old_locus_tag="NUX13_19730"
     CDS             6194..6655
                     /locus_tag="NUX13_RS19725"
                     /old_locus_tag="NUX13_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7091..8059)
                     /locus_tag="NUX13_RS19730"
                     /old_locus_tag="NUX13_19735"
     CDS             complement(7091..8059)
                     /locus_tag="NUX13_RS19730"
                     /old_locus_tag="NUX13_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_012308653.1"
                     /translation="MLESRKEGFSARKFAELIKRHPSTIYRELKRNSINDVYQAQYASD
                     NTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTFPDLDQTMNVSHTTIYS
                     TIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKTIHERPAEVQERKIPGHW
                     EADLIKGKDNKSSIATLIERNTRLCILATLPDAKAESVRKALTEALKYLPAELRKTLTY
                     DRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYL
                     NQVAMSLNTRPRKALDWLTPLEKFAQLVDYHMAFETVAPHV"
                     /name="IS30 family transposase"
     gene            complement(8161..8493)
                     /locus_tag="NUX13_RS19735"
                     /old_locus_tag="NUX13_19740"
     CDS             complement(8161..8493)
                     /locus_tag="NUX13_RS19735"
                     /old_locus_tag="NUX13_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000390243.1"
                     /translation="MEDSTLNELIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNY
                     DKQGVYAFIKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSMAE"
                     /name="hypothetical protein"
     gene            complement(8511..8777)
                     /locus_tag="NUX13_RS19740"
                     /old_locus_tag="NUX13_19745"
     CDS             complement(8511..8777)
                     /locus_tag="NUX13_RS19740"
                     /old_locus_tag="NUX13_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050441450.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERTKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="hypothetical protein"
     gene            8987..10423
                     /gene="mobQ"
                     /locus_tag="NUX13_RS19745"
                     /old_locus_tag="NUX13_19750"
     CDS             8987..10423
                     /gene="mobQ"
                     /locus_tag="NUX13_RS19745"
                     /old_locus_tag="NUX13_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029315.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIDHWREHFADICNVHLNLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRSNDEIKQRNQAQLQYDKNMDVLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KSLFEEKQRGTLGKVLKREISAKDANLDLDFMQRNLKQAEINLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLENEKFKEHAWKKYQKQHPEKAKQYQTLYQSYKIVKKCVDEIKAEQQMKIRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8988..10420
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.213"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagtggtaaa agacaatgcc ttaatcaatg caagctataa cttagattta
       61 gtagaacaac gtttaatttt attggccatt gttgaggcaa gagaaagcgg gaaaggtatt
      121 aatgcgaatg atccccttga agtacatgca gaaggctata tcaatcaatt tggcgtacat
      181 cgcaatacgg cttatcaagc attaaaagat gcctgtaatg atttatttgc aagacaattt
      241 agctatcaaa aaataaatga acgagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaaactta tctttgcccc agccatagtt
      361 cccttaatta cacgcttaga agagcatttc actaaatacg aattgcagca agttagtaat
      421 ctcagcagtg cttatgctgt tcgcttatat gaattattaa ttgcttggag aagtactggc
      481 tctactcctg ttatagagct aagtgatttc cgtcaaagaa ttggcgtact cgatacagag
      541 tacaagcgta tggaacgctt taaaactagt gtacttgagc ttgctattaa acaaattaac
      601 gaacatacag atatcactgt gaagtatgag caacacaaaa gaggtcgatc aatttcagga
      661 ttctctttta cttttaaaca gaaaaagaag gacaacccac cgatagaaag agatccgaat
      721 acgttagatc tctttacaaa gatgactgat gcacaacgcc atatgtttgc aaataaactt
      781 tctgaacttc ctgaaatggg tcgttattcc cagggaactg aaagctatcc gcaatttgct
      841 gttcgtattg ctgagatgct gcaagaccct gaccgaataa aagaactata cccataccta
      901 aaaaaagtgg gatatatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat tccatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tccgatgctt
     1081 tggagaacct gagcaaacat ctcaaaaaat tgaatcatct aggcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc
     1321 gacaacacaa caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt
     1381 agcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc
     1501 cgtctttgat aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg
     1561 ccctgggaat tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg
     1621 ataaaaaggc gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct
     1681 gattattcaa ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt
     1741 gaaaagtcat aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg
     1801 ttgaattata aggataatta tacagcattt cgtataaggt gtattatgtt aattttagga
     1861 aatattaaaa ctaattttca gacttatccc accaactttg agctaaatca gtatttttag
     1921 gaatacctat accatcacga tacataacgg aaagacttcg cattgctttc ttattaccta
     1981 actttgcagc cttttcacga agttcaaaag ctttattaat attaacttct attccgctat
     2041 ctgcacgagt atatagatca gctaactcaa ccattgattc agaatggcca ttttctgagg
     2101 ctttctgaaa ccacttaaaa gctttttgga aatgttctaa agaatcgttt ttgtcatcat
     2161 cttccattaa tctagcataa gtaacatatc catcagctaa ccaaaacatt gctgctacat
     2221 ttccattttc tgctgattta agagcccaaa cttcagcttg attttcatct gaatcaacac
     2281 catgaccata tagataaatc tcggctaatt ctaactgagc agaagtatct ccattttctg
     2341 ctttcgctaa taaatcatta gaaaaaacag gttctgcaaa tgtttccatt gaaaggcaaa
     2401 ctcctaaagc cataaaaata tttgttaatt tattcaattt ttaaccttta aactaaactg
     2461 ttaggcttgt atatttttat atcataccta aaagaaaact ctaaaattag agtatgtctt
     2521 atctctattt aacataatgg cgattatacg aagtacactt gtatattaat ataaatatca
     2581 actacttaat aaaaatagaa aaatcccaaa atgcccaaaa agccgacttt gaaacaagtc
     2641 ggctttttta tgagcagttt tgatacttct tgccaataat tttgactaaa aattacacga
     2701 atctggttga gtgatacaat tttgtgatac attattgtat atacaaattt gaatgttttt
     2761 tatggaacag tattttgaat gggatgaggc taagaatcga aagaatcaga aaaagcatga
     2821 tatctctttc gaaactgcaa gccttgtttt tgaagatcca ttacggatct caatccaaga
     2881 cagacatacc aatggtgaag aacgttggca aaccattgga cgagttaaag gcgtactaat
     2941 gttattggtg gctcacacca tttttgatga agatgactgt gaaatcatcc gaatcattag
     3001 tgcaaggcaa gtcactaaag cggagcgaaa caaatatgag catggttaga tactcacaca
     3061 aagagctgaa tgagaaattt ggtgagaagc aagatgctga aattcaacgc ttgcttgcta
     3121 aaggcacggt tcctgatgat cagctagatc tatcagatat tcctgaaatt acagactgga
     3181 gcaatgctgt acgccaaaat caattttacc gcccagtaaa gcaacaaact tcaattcggt
     3241 tagatgctga tgtacttgct tggttcaaag cacaaggcaa aggttatcaa acacgtatga
     3301 atgaaatttt acgagatgct atgctaaaag aattaaaaaa tcatcaataa gaatgggagc
     3361 ttaggctccc atttttgact tcatttttag gggggattac cattcaaaaa tttaaatagt
     3421 agattttata aatttttaag tgttatatta taatataaca ttaaatttgg ttgttttatg
     3481 ctaaataaaa gtaaactatt tttagcttta attactttag gggcctcgaa aattttattg
     3541 gcagctgaag ggcctgttac taccttaaat acaatagtat taactgcaca atctgatgag
     3601 ttagggtctg agctgttagg caaaagtcta aatgttagta atcagtttat tgatactagt
     3661 aaacttaaac agcgttcgac aactctaggg gatgccttgg gtacggaatt aggaattcat
     3721 tctaatcaat atggaggtgg agcatctact cctattattc gtgggcagga aggaaaacgt
     3781 attaaagttt tacagaataa tgctgacgtg ttggatatgt ctaatatgtc cccagatcac
     3841 gctgtcacag ttgaaccaag tttagctaag tctattgaaa ttatacgtgg tgcaagtacc
     3901 cttttgtata gttcaaatag tgcggcaggg gtagtcaatg tcattgacta taaaattcca
     3961 acccaaatgc ctcaagacgg attagagggt aatactacat tacgttttaa tactggcagt
     4021 aatgagaagt tgaccacagc aggtgtaaca gttggcttaa gcccacatgt agctttacgg
     4081 gcagaagggt tatatcgtaa tgctggtaac tataaaactc ctcattacca gtctagctct
     4141 tataattctt tagaagatct tgaaaaccaa aacagtattt ataaaaattt aaaatattta
     4201 ccagaaagct gggcagagtc gagagtaggt acgcttggat tatcctggat agacgataat
     4261 acttatttag gagtttctta tacacatcgt catgatgaat atggtttacc agcccatagc
     4321 catttatatg aaggttgtgg tgcctctgca atcggtatcg attcacgaat tagtggatta
     4381 aaaaattatc ttttatatta tcctcaattg atggatgagc aagatattaa ttacataaat
     4441 ccacgtcctg attgtcatca gcataatcat attcatgaaa ctaatttctc tcataatgct
     4501 ccttatattg atttaaatac tcgacgttat gatgtgcgtg gagaattcac tcaaccattt
     4561 accggtattg acaaaattcg tacctcattg agttatatag attattttca caatgaactc
     4621 gagggagata agattactaa cttttttaaa aatacaggaa aagtaggccg catagagtta
     4681 tcacatcagc cattagggga attaaccggt atcttggggc tacaatattt ggaacaggat
     4741 aatagtgctt tatcaccagt ccattctcaa gaaggccata ccacctatct agataatcag
     4801 cagctattaa atcgaaatgt cactaaaaac tttagtgtat ttggcctaga aaaatataac
     4861 tggaacgact ttactttcga actaggtgct cgtatagaaa agcagaaagt tagtatggat
     4921 tatgatatag aaaaaattaa agattcaatg aaaccttggc ctaataaata taacagtcct
     4981 tatgtagaaa aaaataataa aatcagggct caaaatttaa aatctatttt agaagctgtt
     5041 caacctaaca aagaaacagc tttttcttat gcaggtacag tccactggag atttgctcca
     5101 aattatatct tatcattaac aggtacacat caagaaagat tacctaatgc acaagaaatg
     5161 tatactcatg gtatgcatct cgctactaat tcttttgaaa taggtaaccg ctttctaaga
     5221 aaagagaaat ccaataattt agaaatcagt ctggcctata aggatgattt acttgattat
     5281 caaatcagta cttactatta tgattttgat aactatatat atttacagac actcaatgaa
     5341 gttttaggaa ctacaaaagt tagagatcag catacactac gtattaatca ttatagccag
     5401 tctgctgcta atttttatgg attagagggc aacatagggt atcaatttaa ttctgtttat
     5461 cacggctctc tttttggaga ttatgtgaaa ggccgtttaa caaatttacc tgatgctgta
     5521 attgcatacg atatctggaa tagagaaccg actttagctc cccaaaagga tcgttacact
     5581 cctagattac ctcctgctcg tttgggtaca cgactaaaag ctgattttga tgaatcgctt
     5641 aaaggtgaaa tagaatatta tcgagtcttt aaacaagata atatttctaa atttgagcag
     5701 gttacatcag gttataatat gcttaatatg actttagcat ataaaaataa gctatctcac
     5761 acagaatatg atttattctt taaagcgaat aatttacttg atcagaaagt ctatgcccat
     5821 gaaacatttt tgccatatat tccccaaatt ggaagaaatt ttagtttagg cttaaatctt
     5881 aatttctaga taagatactg taatcttcta agaaaatttc ttagaagatt tttcttataa
     5941 aatgattaga ttaataatta aggtaagcgt ccgctgaatc ccatacctat tttttaattc
     6001 gatttaggtt tccagcagtg tggcagtaac tcttcaatct gggtcacttt atgtgtcggc
     6061 agccttttca gcactgaata gccaccgatt ttgtagacac cttttagcta gataaaaagc
     6121 aatttttttg atctttaaag aatgaggata taagctgttt aaaacttaat tctaattggt
     6181 taataggtga aaaatgaaat tagcagaagc tctcttactt cgaagtgatc aacaaaaaaa
     6241 aattatttca ctaaaacaac gtatcaatgc aaatgtattg gtacaagatg gtgatcaacc
     6301 atctgaagat ccaaatgaac tactaaaaca agtattttct ttaattcaag aatttcaaaa
     6361 attaagttat gcaattcatg aaactaatgc tttaacaaaa ttaaatgatg gccgttctct
     6421 acttgcttta ttgactttgc gtgatgaatt tgtagaacag cacaaaaccc taactgcagc
     6481 aataagtaat acatctcgtg aatctgatcg ttatagtacg cgggaaataa agtggcataa
     6541 agttattcca gtttcatcat tacaaaaaca agctgatgat attagtctta aattacgtga
     6601 tttaaacgtg ttaattcagt cgactaattg gaaaattgat ttgattgaag cctaatagtc
     6661 ctttagatat aatcaagttg aacatactgg gcgagtacaa gatcccttac ttccacaaca
     6721 taggattgta aataaagtgt agtggcttgc aaagcaagtg gataagctta aaattttaag
     6781 tacaaatcaa tccatcactt ggcataactc aaatctttgt gcaaacaaag ttacacgtta
     6841 cttataacta ccatgtactg acagtatgtt cacctcaatt actaaaaata aagaaatgaa
     6901 caagcatcag ctcaactaca tttctatttt taattcagtt cggcatttaa cataaaatct
     6961 cttatgcgaa attacatacc aactttaacc acattgtgga caggttggag tttagagata
     7021 agaaaaattt ctagagcatt tgctaaataa gccctatcac gttcaaagcc tatcgtaatg
     7081 atgggcgaat tcaaacatga ggtgcgacag tttcaaaagc catatgataa tcaacaagct
     7141 gagcaaattt ctctaatggt gtaagccaat ctaacgcctt tctaggacga gtattcagtg
     7201 acatggcaac ttgatttaaa taatgctgat ctgcctgatt taaatcaatc cctttaggta
     7261 aatattgcct aattaaacca ttcatatttt cgcatgtgcc tttttgccag ggtgaatgtg
     7321 ggtcacagaa atatacatct atgcctaaat cttcttcgag tattttatgt tctgacatct
     7381 cacgtccacg gtcataggtc aacgttttac gcagttctgc aggtaaatat ttcagagctt
     7441 cagttaaagc cttgcgcact gattctgcct ttgcatcagg taatgttgcc aagatacaga
     7501 gccgtgtatt tcgttcaata agtgttgcta tcgaactttt attgtcttta cctttaatta
     7561 aatcagcttc ccaatgaccc ggtatttttc tttcttgaac ttcggctggg cgctcatgaa
     7621 tagttttaat atcctgtaat atagaatctt ttttaggttc accgttagct tttcgctttt
     7681 tattttcatg acgtagacag gataataagt cttttttcaa ctcacccttg ggtaatgctc
     7741 gtatcgttga ataaatcgtt gtatggctta cattcattgt ttgatccaaa tcaggaaatg
     7801 tctttaaacg ctttgctatt tgctgaggag accataaaca acggatcgct tcaacaataa
     7861 atttccagag gattgaatcg attttgagtt ttctgtgacc acgtctacgt ctagcaaaag
     7921 tgttatcaga agcatattga gcttgataaa cgtcattgat gctatttctt ttaagctcac
     7981 gatagatcgt actaggatgt cttttaataa gttcagcaaa ttttctggct gaaaagcctt
     8041 cttttcttga ctcaagcatt aatgcagtac gatcttcaaa gttaagatga tggtatgaca
     8101 attttatata ctccataaac cctttaaatt aattaggtgg tttatgtcgc acttcaagtt
     8161 ttactctgcc atggatccag catcttttaa tcttggatct atcgcaatat attcagtttt
     8221 atcttcattc catcggcaat atttcattac cctggcgctt aagccattac cacgataacg
     8281 gccagcacct ctagaagtgc caacaccgat ataagtcact aaatcccctt tgataaacgc
     8341 atatacacct tgtttatcgt aattaggaat tttcccaaaa ttaaaatgta ctggtgacca
     8401 ttcaggaggc tttcctaata gatctacatt ccagtgatgt tcaaagaata attctgtatt
     8461 acgtattaac tcattaagtg ttgaatcttc cactactcat ctctcaaaat ttatagatta
     8521 aatagctttc ggtcacgctc atccgttaaa tacttatcca tctgagcaat tagtttagct
     8581 ctggcttgtt catcatcttc agtgattttt aacatacagg atccaattaa aatcttgcgt
     8641 ctggtatcgt cctttcgtgc ctgttctttc tctttggtac gctctcttgc cagtgctgcc
     8701 tgtctttgag cctttaactg ctttagcttt tcttcctgtg ccttgatttt gctgtctagt
     8761 gtttcagtaa cgctcattaa tccaatacca tcattctggg aaaccatccc taacataagc
     8821 gcacttgaat atgcattcaa ggtgctatat gtatagtaat gttttcctcg aagagcgcac
     8881 ttatgcaaac tatgtttgca agtccgattg gggtctcccc aataccccga caaccgtatt
     8941 cacggttgta tttcactacg tgaaaattta aaagcgaaaa atagacatgg cgatttacca
     9001 cttttcagtc aaaaccgtag cacgatcagc agggcgttcc gccactgctg cgattgccta
     9061 tcgggcaggt gaaaagatct attgtgaacg tgaaggccga gaacatgact acagccgaaa
     9121 aaccggtgtg gaatacaaag aaatttattt accagaaggc gcaccagagc atttaaagaa
     9181 tcgagaaaaa ctctggaacg aagttgaaca acgggagacc cgaaaaaact cgactgtagc
     9241 cagggaattt gaaattgcct ttccaagtga actgaaccag gaacaacgtc tggccatgct
     9301 cgaagaatta tgtgccagca tcgtggaaag acatcaagta gcagtcgatg cctgtattca
     9361 tgcaccgcat accggatccg gcagtgatga gcgcaattat cacgcccata tcctaatgag
     9421 cacacgtaaa cttacgccag aaggttttac agagaaaacc cgggaactcg atcagaaaca
     9481 cagtggagaa atcgatcact ggcgtgaaca ctttgccgat atctgcaatg tgcacctgaa
     9541 tctggccgga tccacagccc gggtcgacca ccgcagctac aaagaccagg agaatggcct
     9601 ggaagccacc ctgcacgaag gaccgaaagt caccgaacta cgtagaagag gcatcgaaac
     9661 cgaaatcagc cgaagcaatg atgaaatcaa acagagaaat caggctcagt tgcaatacga
     9721 caaaaatatg gacgttctaa ttgctgaaaa tgagataaaa ctcagcacac tgaaaacaga
     9781 acagcagatc caaatcaaga atagcgctaa aacgccacca attgacgaaa aatccctgtt
     9841 tgaggaaaaa cagaggggaa cccttggcaa agtgctaaaa cgcgaaatca gcgcaaaaga
     9901 cgcgaatcta gacctggatt ttatgcagcg caatctcaaa caagccgaaa taaacctgac
     9961 caagcaccac aaacatcaaa acgagttcaa tcagcagctt gcccaggaga tcgtgaaaag
    10021 cggactcaag caaagtcatg acaaattgca aagcctggtc gatcaacaca acgaattaac
    10081 ccagaacaaa cccttactgt ttggaaaaaa agcctgggaa gcccaacgtg atgagatcta
    10141 ccaggaacac aaaaagttga aaggtcagca tgaacaccag aaaaaacatg gtgtgaagga
    10201 tttattggag aatgaaaagt tcaaagaaca tgcctggaag aaatatcaaa aacagcatcc
    10261 ggagaaagcc aagcaatacc agaccctcta ccagtcctac aagatcgtta aaaaatgtgt
    10321 ggatgagatt aaagcagaac aacagatgaa aatcagacaa gaacaacaac tcaaagcaca
    10381 gcaacatgcg cctaaaatga aatctcgtgg catgagtcgt taaacagagc gagtgtctac
    10441 gagcgaactg agaaaatttg cccatccctt tccctgatta caagctccct atccatcaat
    10501 cacctgcata ccagagcatc ccgcatgggt gcgaactttc caagtttgat tcctaaatcg
    10561 agcgtagcga gaaaaaaagc tcatgagcga agcgaattcc gagttgcttt tgatcttgct
    10621 tttgcttttt ctcaaattca cgaaaatatg aaccaaaaat tgccccgacg aatcgagcga
    10681 aagcgagatt caatagagtt tgagcgaacg aagtgaagcg aaaaccaagg gcaatttttc
    10741 actccttggg cttttaatta ttttaaagtt tttaaatgct tttagatatg ctgaaagcct
    10801 ttgttcacaa tgcttttaag gtttatatga caccgtttac cttgcaatat gacaccgttt
    10861 accttgcaat atgacaccgt ttaccttgca atatgacacc gtttaccttg cttatagcac
    10921 taaaattaat tattgtgtca tagcataata attaatactg gtgcttc
//
