LOCUS       NZ_CP102558             8179 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KPH3 plasmid p6, complete sequence.
ACCESSION   NZ_CP102558
VERSION     NZ_CP102558.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30176386
            Assembly: GCF_024666505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPH3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:16:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,472
            CDSs (total)                      :: 5,350
            Genes (coding)                    :: 5,243
            CDSs (with protein)               :: 5,243
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 10 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102558.1.
            Bacteria and source DNA available from Human.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPH3"
                     /isolation_source="microbial"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="15.8700 N 100.9925 E"
                     /collection_date="2020"
                     /collected_by="Faculty of Medicine, Chulalongkorn
                     University"
     gene            complement(join(8153..8179,1..237))
                     /locus_tag="NUG48_RS27285"
                     /old_locus_tag="NUG48_27290"
     CDS             complement(join(8153..8179,1..237))
                     /locus_tag="NUG48_RS27285"
                     /old_locus_tag="NUG48_27290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257874041.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASWGRFRLRAK"
                     /name="hypothetical protein"
     gene            complement(1024..1434)
                     /locus_tag="NUG48_RS27290"
                     /old_locus_tag="NUG48_27295"
     CDS             complement(1024..1434)
                     /locus_tag="NUG48_RS27290"
                     /old_locus_tag="NUG48_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1434..1625)
                     /locus_tag="NUG48_RS27295"
                     /old_locus_tag="NUG48_27300"
     CDS             complement(1434..1625)
                     /locus_tag="NUG48_RS27295"
                     /old_locus_tag="NUG48_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2454..2810)
                     /locus_tag="NUG48_RS27300"
                     /old_locus_tag="NUG48_27305"
     CDS             complement(2454..2810)
                     /locus_tag="NUG48_RS27300"
                     /old_locus_tag="NUG48_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(3014..3334)
                     /locus_tag="NUG48_RS27305"
                     /old_locus_tag="NUG48_27310"
     CDS             complement(3014..3334)
                     /locus_tag="NUG48_RS27305"
                     /old_locus_tag="NUG48_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3517..3759
                     /locus_tag="NUG48_RS27310"
                     /old_locus_tag="NUG48_27315"
     CDS             3517..3759
                     /locus_tag="NUG48_RS27310"
                     /old_locus_tag="NUG48_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            complement(4551..7448)
                     /locus_tag="NUG48_RS27315"
                     /old_locus_tag="NUG48_27320"
     CDS             complement(4551..7448)
                     /locus_tag="NUG48_RS27315"
                     /old_locus_tag="NUG48_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            7543..8148
                     /locus_tag="NUG48_RS27320"
                     /old_locus_tag="NUG48_27325"
     CDS             7543..8148
                     /locus_tag="NUG48_RS27320"
                     /old_locus_tag="NUG48_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          1745..2154
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccaggaagcc tggagttcac cgcgttctat ttttaacttt tcacgataac tgtctggtgt
       61 gtgtagttcg ttcccatccg catcgagatt aataacttcg cctcgctcac caacagtaac
      121 aaagctctct acgtgagaca gatcatcaac aaccatcaca ttccttttca ttcttaaacc
      181 agtcactttt gcacctgcac cgatcttcat aaaactaaag cctgatttat cgttcactca
      241 catcacctca taagataaaa atgttaataa cgttttcacg agcaattatt taccataacg
      301 cctcattacg cgcaccgctc aaacgctcta gaaacgattc tggggcgtaa tccattaaat
      361 cctacgtctt accccctgaa aacgcctcaa aatcgctcca gggcgtttta cgggcatgaa
      421 ttgttatgca ccagactgac tggtggcgga tttcagcctg aattcaggcc agacaactca
      481 aaacccgcac caaaccagac tgaaaacggt gaacggggac gcaatttatt gcgtggccgt
      541 gaagcgttgc cggcgcagcc ggaaaaacgg ttttcagcac gttcaggaat cgggttcgtc
      601 gacggccggc aatcacgtta tgcaagtcgc cggacgactc accacacgat tcaccggcga
      661 ttcgactcgt gacttgactc tagagtcgcc aaggtcaaaa aaacaccaaa atgacgattc
      721 gactctagag tcaagtcgtc ataagttact gatagtctta agtgagtcgt cgatgactct
      781 atgacgagtc gtcggcaagt cgtacacgaa tcgccgacga cttggattca ccttacagaa
      841 agtaatactg cgtaatgtaa taacccacaa ctaactgata ttaatgaatt atcttcaaaa
      901 ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctggcg
      961 atttttggga aggttcagga actccccggg atggccggag aggaaaacgg aagcatgacg
     1021 gagtcaggat tttttccaga atttccacca cggggaatcg ggtggtgaag aaggttcgtg
     1081 cttgtgctcc agaagctgca tagcctgacg aagggaatca atatgctcat ctcgagcctt
     1141 aacagcccct ttaagaccat cgttttcagc ttctagtaaa gcaattctat gttgtaaatc
     1201 atgtaaacca acgctgttaa cacctgtaga tcctgcgtca ctttttacag tatcgatgac
     1261 accatcaaga gaaccaaaaa ctctaataag ctctgaagta tcaataactt taatgatctt
     1321 cccgtgcttt acaacagagg tgtaacttaa cttcccactg ttaatcatct tgtaaattgt
     1381 ggggcgacta accttaacaa gccgtgccgc ttcactaata gacactctgg ccatcagtta
     1441 ccatcctgta attcatccag tctcgaggac agtgtacgga aaagatgctc cgcatcatcg
     1501 tggagtttct cacaaaggtc ggcctctgca tcaagatcaa gctcgttaag cttctccagc
     1561 aggagcagag actgattctg gatgaattta gccatgttca gcgctgtttt ctgttgtttc
     1621 gtcataagaa gcctcagagc gtcagtgctc agtgtaaaac cagtctcttt tccgcttcct
     1681 cgctcactca gtcgctacgc tcctgccgtt cggctgcggc gagcggtagg actatgcgta
     1741 taaatgctcg agaaaaccga gcggacgcgt gatgggcaga aggaaaggcc gcttagcggc
     1801 cgtttttcca taggctccgc ccccctgaca agcatcatag aaattgacgc tcaaatcagt
     1861 ggtggcgaaa cccgacagga ctataaagat atcaggcgtt tccccctgat ggctccctcg
     1921 tgcgctctcc tgttcctgcc attcggttta ccggtgtcat tccgctgtta tggccgcgtt
     1981 tatctcattc cacgccagac actcagttcc gggaaggcag ttcgctccaa gctggactgt
     2041 atgcacgaac cccccgttca gtccgactgc tgcgtcttat ccggtaacta tcgtcttgag
     2101 tccaacccgg taagacatgc aaaagcgcca ctggcagcag ccactggtaa ctgatttaga
     2161 ggagttagtc ttgaagtcat gcgcctgtta aggctaaact gaaagaacaa gttttggtga
     2221 ctgcgctcct ccaagccagt taccttggtt caatcacctg caagtgtttg aaccttcgga
     2281 aaaaccgccc tgcaaggcgg ttttttcgtt tacagagcaa gagattacgc gtaccgaaac
     2341 cggatctcaa gtgctcgtaa aagaaaatga tgatgacctt tagcggttac aaaaaaagaa
     2401 ggcacactat tacagaggcc ttcttctagt ttggttaaat ttttttaaaa aaatcatgcg
     2461 accgcatgtt ttttttccaa agattcagca ttgtgcttcc tttctataat ttcatgagcg
     2521 ggagtgactc tttctatttc ttcctgaagg cgtgggctgc tcgtaacctc ccacaaatca
     2581 acagactgct gtatatttaa ccagaactta gcggtggtat cgaatgcttt agctaaccga
     2641 aacgccatat cggtagacag tttcctatta ttgttaacca aagcacttgc agaatttcta
     2701 tgcacgttta acatttcagc caagtcattg atttttaaac cacttggctc gagatattca
     2761 taaagaataa tatctcctac agtcgatggt tttctggttg cttgggacat gctttactcc
     2821 tgaattggtc gcttctctct gttagatgag gaagataagt atttcttctg ttaccagctg
     2881 tatcgattag taattatctt aaatatagtt acgtcgcgtt actttattaa tcattatttg
     2941 tgatgacgtt ttaacgtagg tgaggaaatg cctcacctac ttgttttgta agataacagt
     3001 tccggtctgt tacctatcgg taatcatgtg gatcgagata caaatcctcg actccgttct
     3061 caccccactt aaatatcaag cggtacttat cattcaccct tatagatgaa taccctgtca
     3121 atggagggtt gagattttca aatcggttcc ccgggggaga ccgtaaatct ccaattttat
     3181 cagcagcatt cagcatatcc agcttgcgac ctaatgaaac actaatcgta gctggaattt
     3241 ttcgattggc tttaccgtat ttataaaact ggtatagcca atcgtctcgg aactgaatgc
     3301 ggcaatcatc atcactatca tgattttgct gcatttttct tttacctcaa atgttgccgc
     3361 aacttggcaa gttctagatc accttttacc accaccatag tacaacaaac gccagaaatg
     3421 cacaatagca ctgtgcactt gtgcaaaaaa ccataatgat ataaaatgca caaaagcaca
     3481 gtgctactgt gcaaaaaaag aatagttgga ggtcatatgg cgcttgttcg ctcaggaaat
     3541 aaaaccaaga tttttgaagc agatccgaaa tttaacccag tttttgatac acaacacaag
     3601 cctggcgata cgtgcacgta tgaaggaatt tacaaatgca caggttgcgg taaagaagta
     3661 actatcgcag gagggaaaac acttccacca caaaatcatc atcaacatag tcccggacag
     3721 ggggacatcc gctggcaact atttgtttat gcagagtgag gtatgaaaat ggcagaccgt
     3781 agaccactta acccgcatgt cgtcgtccct aaaccaggag gcggaactca gccacgagat
     3841 cgtaataata atggaagagt ccgagagaaa cgttcagatg cgggaaaatc aagaaaataa
     3901 gaaaagccgc caaatagggg tcagtttgga tatcggaaac ttttgtacgt taagcgcatt
     3961 atttaaccaa tattgatgcg taagggattg attttgcctc agctacgatc gcttttaagt
     4021 caccgctctt tctgtatcgc atttaggtta tccgattaag gaaaattcac gggaaacggc
     4081 gtatagcgcg ggagtacatc attgcgagag gtaagcctgt tcaaaacaca ggcttaccgt
     4141 acaataattc tctatatcca aactgacccc aagaagtctg acactaaccc cacacaaatc
     4201 gaatccgttg caagatgaga cacacaagaa aagagctaga cgaaggtcgc tccgctcccc
     4261 atggtttttg tatacccacc atgggggcat aggtagttta ttaactaact ccaagataat
     4321 gcgtgatcgc agccgtgagt acaccacagc ccacttgttt agataaatct aaaacccctt
     4381 gtaggattct cttagtgttc cactcacctt ctgcatccat tggttgagat agtgcagcac
     4441 tcaactggtt cttaatcttt tgcgaagtta tatcgttatc tattgagttg atttcctcct
     4501 tggctttctc caaggaaggg gtcggttccg gctgagggcg aaatgacacc ctaagctttc
     4561 ggttccttgg gccaaagata ttcgccagtc agtagaatgt gcgcccagcc caatggggat
     4621 atgtggggaa gaaattcagg gggaacatcc aacccttcgt tccgccgctc cgtgacggca
     4681 tgaccaagat ggacggtatt ccagtaaatg atcaccgcag tcaataaatt gagcccagcg
     4741 attcggtagt gctgcccctc tgtcgtgcga tcgcgaattt ccccctgcct cccgatacgg
     4801 agcgcatttt tgagcgcatg gtgggcctct cccttgttaa gaccgatctg agcacgccgc
     4861 tgcatgtccg tatccaggat ccactcaata atgaaaaggg tccgttcaat acgaccaact
     4921 tcacgaagcg caactgcaag gttgttttgt cgtgggtaag aagcgagctt gcgcaggagt
     4981 tggctgggcc tgattttgcc agcggtcatc gtcgcggcac aacggaaaat atcaggccag
     5041 ttcgcaacga taagatcctc ccgggctttt ccacctacca acttgcgtaa ctccctgggg
     5101 gtcgtatcgg gattaaatac gtacaaccgc ttcgatggca gatccctgat tcgcagaacg
     5161 agattgtagc cgagcaggct actggctccg aacaaatggt cggtgaatcc tgctgtatcg
     5221 gcatactgtt cgcgaacatg gcgaccgacc tcgttcatca gtagtccatc gagaatatac
     5281 ggtgcctcgc tcacggtcgc cgggatcgac tgacaagcga atggcgcgaa ctggtcgctt
     5341 acgtgagtat acgctttgag gccgggaaca gaaccatatt tggcattgac catgttcatg
     5401 gcttcgccat gccgcgctgt cgggaaaaac tgaccatcgc tcgatgctga cgtgcccatc
     5461 ccccagacgc gtgacatcgg cagtttaccc tgcgcggcca ccacaattgc caatgcctgg
     5521 ttcatggctt cgctttcaac atgccagcgg gcaaggcgtg agagctgcca gtaatcatgc
     5581 gtgtttgtag cttccgccat cttacgcagg cccagattga gcccttcagc gagcaggacg
     5641 ttgagcagac cgatccggtc gcgacatgga gccccggttc tcagatgggt aaacgcatct
     5701 gtgaaaccaa gggctgcatc aacttcaagc agcatgtcgg taatccgaac ggacggcatt
     5761 cggcgataca gatccagtat gagtgcctcg gcaccatccg gcacgtctgc tgtcaacctg
     5821 tcgatccgca acgttccatc ttctatgcta ccgtgcggaa tagtgccgtt acgggcagcc
     5881 cgggccagcc gcttaagagc gatcgtgagt cgcgcctttc tgtctgccag ccaatcctgt
     5941 gggttggaag gcacggccag ttttgcattt tcctgcgccg cgatcatcgg caccagtacc
     6001 tgcttgaggt caccatagcg gcgcgaatga gcgagccaga catctccgga acgaaaagca
     6061 tcccggaggt gaaagagtac cgccacttcc caaagacggg tatctccttt ttcctgagct
     6121 cgtaaatgac ggttccattt ggagctgggc cgcaggaaac gcctttctgg cgatgcaaca
     6181 cctttcatct ctccgatcga caaagctgct gctaccaatg gtccggcgac cggcgcggct
     6241 tcgagcttca gacagcgcaa catgcggggc gcataacgac gaaagcgatg gtatccctgc
     6301 ccgacatatg caagaggctc atcggctagc gtgttgctga gttgagtccc tgtcgctacc
     6361 agttgagcga gccggtccca tgcaaccgaa ctggcgacag ccatctccag cggggttccg
     6421 tcactgcggg cctcaagcaa cgaagctccc agcgcggtga aggtacggat cgtatccgtg
     6481 agtgtggctt tagagccgga aattgtttcg tcatgctggc gcttcgcttc ccgccaggtt
     6541 tttcctacga tcctgtcatg ggtttcgact atggcatcag caatcgccgc ttcccactcc
     6601 acaacacaga cggcaaggat cgcccagcgg cggtccgaag tgatgtcacg caaaccgtcg
     6661 gtgaagtagc gttcaccctg ccgacgcagc cgggcaatgc gatgggcagg tatgctggcc
     6721 aaagcactat gattgatatt cagggtacgc agaaattcga gcctgtcgag caaacggtta
     6781 gcagcagccg agttgttacc aacctcgaag ttgcgaagcc agatgaaacg actgatattg
     6841 ccggcgagca tttcactcag aagtttgtcc aggtgatcgc gaacatccgc tgttaaattt
     6901 tccgcaatcc gcgtttcaat ccgccgctca gcggcgacca gagcatccgc gcacaagcgc
     6961 tcgattgtcg atactgcggg cagaatggtg gaagtttccc gacaccgcac aataaaacga
     7021 tgagcaagat cctcgtttga tctggcatct tcggcctggc cgaaagtcca ctcccgcaga
     7081 tcacgggcac cacggcccgt gaaggtcttg tagccgtaaa tttcgcgcag cgtgtccatg
     7141 tgctgctgac gggtttggcg ccgtgtggca taagtgagaa gcgcatcagc cggaactcca
     7201 agctgagcac cgacgaagga aaggacttca cgcgggatca tctcaccagg agccagtgca
     7261 cggcccggat atcgtaaggc acaaagttgc agggcaaagc caatcctgtt ttccggtctg
     7321 cggcgctgcc taatgttttc caggtcatca tcgcccagcg tgtagaactt cagtagcgac
     7381 agttcgtccg tgggcagatc gaacagcgct gctcgctgcc gttcggtgaa aatatggcgt
     7441 cgtgacatac aaattcgtcc cttttgaagt atagtctgtt ttggacaaca gccagcccat
     7501 ataaatcagg gcgttccgat acaaaaatcc aggagggttc aattgggaca tcgtgccgcc
     7561 atttactgcc gggtttcaac agcggatcag tcttgtgaac gccaggaatt tgatctgcga
     7621 gccttcgccg gccgtgccgg ctacgacgtg gtgggaatat ttaaggaaac aggttcagga
     7681 actaaactcg accgggccga gcgaaagaaa gtcctggcgc ttgcccagtc cagacaaatt
     7741 gatgcaatcc tggtcactga gctttcccgg tgggggcgct cgacgctcga tctgctcaat
     7801 acgctacgtg aactggagaa ctggaaggtt tccgtgatag ccatgaatgg aatggcgttc
     7861 gatctttcgt cgccgtatgg acgaatgctg gcgacgtttc tttccggcat tgcggagttt
     7921 gagcgggatc tcatcagcga gcgggtcaag tcaggccttg ctgttgcgaa ggcacgtggt
     7981 aagaggcttg gtcgtcaggc cggagtgcga ccaaaatcag accgactttt gcctaaggtg
     8041 gttgcgatga gggccgaggg acgcagctat cgctggatcg cacgcgagct cggtatcagc
     8101 aagaataccg tcgctgacat cgtgcaacga cacagagcta acgcttaggg tgtcatttcg
     8161 ccctcagccg gaaccgacc
//
