LOCUS       NZ_CP102557             8660 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KPH3 plasmid p5, complete sequence.
ACCESSION   NZ_CP102557
VERSION     NZ_CP102557.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30176386
            Assembly: GCF_024666505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPH3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:16:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,472
            CDSs (total)                      :: 5,350
            Genes (coding)                    :: 5,243
            CDSs (with protein)               :: 5,243
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 10 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102557.1.
            Bacteria and source DNA available from Human.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPH3"
                     /isolation_source="microbial"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="15.8700 N 100.9925 E"
                     /collection_date="2020"
                     /collected_by="Faculty of Medicine, Chulalongkorn
                     University"
     gene            237..641
                     /locus_tag="NUG48_RS27245"
                     /old_locus_tag="NUG48_27250"
     CDS             237..641
                     /locus_tag="NUG48_RS27245"
                     /old_locus_tag="NUG48_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(735..1166)
                     /locus_tag="NUG48_RS27250"
                     /old_locus_tag="NUG48_27255"
     CDS             complement(735..1166)
                     /locus_tag="NUG48_RS27250"
                     /old_locus_tag="NUG48_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            1584..1841
                     /locus_tag="NUG48_RS27255"
                     /old_locus_tag="NUG48_27260"
     CDS             1584..1841
                     /locus_tag="NUG48_RS27255"
                     /old_locus_tag="NUG48_27260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            complement(1871..2929)
                     /locus_tag="NUG48_RS27260"
                     /old_locus_tag="NUG48_27265"
     CDS             complement(1871..2929)
                     /locus_tag="NUG48_RS27260"
                     /old_locus_tag="NUG48_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(3259..3425)
                     /locus_tag="NUG48_RS27375"
                     /pseudo=""
     CDS             complement(3259..3425)
                     /locus_tag="NUG48_RS27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3841..3990
                     /locus_tag="NUG48_RS27265"
                     /old_locus_tag="NUG48_27270"
     CDS             3841..3990
                     /locus_tag="NUG48_RS27265"
                     /old_locus_tag="NUG48_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_257874040.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDG
                     VGSG"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4006..6903)
                     /locus_tag="NUG48_RS27270"
                     /old_locus_tag="NUG48_27275"
     CDS             complement(4006..6903)
                     /locus_tag="NUG48_RS27270"
                     /old_locus_tag="NUG48_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            6998..7603
                     /locus_tag="NUG48_RS27275"
                     /old_locus_tag="NUG48_27280"
     CDS             6998..7603
                     /locus_tag="NUG48_RS27275"
                     /old_locus_tag="NUG48_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            7701..8102
                     /locus_tag="NUG48_RS27280"
                     /old_locus_tag="NUG48_27285"
     CDS             7701..8102
                     /locus_tag="NUG48_RS27280"
                     /old_locus_tag="NUG48_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3318..3720)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 tctgaatggc gtgaatattc gcgctatgca cttgcgtgca tactcatgca tgccataaaa
       61 acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc
      121 ccgtcagaaa cgccctcagc gcgtttctga cgatgctgat aacggatgtt atggtaaatt
      181 atccgcttgt ctgaaagctg gctgccgaag caaccaagga aaggagaaca gacctgatga
      241 ccattttagt gacgatacag gcccagctcg ttgtcgggga ggaacaggtt ataaagagcc
      301 tcgctcctga gggcatgctg gccgccgtgt tcgaagacga tggccggacc gggtatttct
      361 atgcgctcga tgagtccgtc gagggaaacc cgattatgga cgcagtacac atctacaatg
      421 ccgaagacgt ttcagacggt cacattcctt ctgatgttaa aattggctgg tcagaagaca
      481 gtcagaagtg cgtactcctg atcaatggat acccgcacgg ggcattcgat ttcgtgggaa
      541 aaaacgggta ctgcaggagt ggttatcctc cccctataaa caaggtgtgg tctctttccg
      601 ggcatgagtg gagcgactct gtggatgatt ttttccggtg aattacggca ggctatagcc
      661 tgccattatg gttcgaaaaa cggatgttat ggtaaattcg tctgtgaagt aggtagacat
      721 ttctaaagct agttttaata gccaagtctc ttcaattcag tcgagaggcg tttgaggctt
      781 acatcaagat tgcttttatt ttgttttaac tcttttttca taaactcagt gtgatgtgct
      841 agctcagggt tatcagttga gcgagtcacg ggaaaatcat cactggatga aagtcgccca
      901 tttttactgt agtccatggt gatacagatc agagttgcaa ggtcgattac ccggtgaaga
      961 ggcagctctt ctgattgtct tgaccatttt tctcctgtgt ttcgccaaac ctttgccgaa
     1021 agctcagtcc atccaggagt gttccattga gcaataccta cagatagccc catagcatca
     1081 gtgtcatcgg catgtgggcc gtcaatgcgg tcgtaatttt cgacttgaat aattggttta
     1141 tgcttcaaat gcgcgggtat tttcatcagt tcgtccttag gtaatgtttg tgattattaa
     1201 attacattgt ttttctaatt ataaaactca ctaaatgtaa gtaaatatta atatctattg
     1261 ttatcatgtg cttgtgattt tgtttttcca attttatgtt ttttgcttgg agagtagcgg
     1321 tgtttttttg tgtcaagtgt tatgataagc cgtacgtttt ttttctggtt tatagttcgg
     1381 ttattcacag caatgggcgt ggttttctat gactacagat aacgttagga atattggcga
     1441 ctataaaaag aaagatgtcg gggtggatgc tgactgtaat ccagtctcag gaaacacacc
     1501 accagtcaga ctaagtgaga aacttagaaa tggaattcct cctggtttga aaactcagat
     1561 gcgccaacaa atgaggaaaa aacatgggct ctaacaataa tcagaataaa gatctgcagg
     1621 ataagcaagc tgatcttttg gaccaaacgt atactcttgg ttggatggat ggctatgccg
     1681 aagggtatga tgatgctatt gaagatactc agaacgcttt gaataaactg gaaaacgaaa
     1741 acgagttgct tactgaggtt gttgaaagta aagataatgt aatcaaagag ttggcctcta
     1801 gtgctggcag ggcgctgcaa gcagtaaaga aaaacaaata aaaaaaggcc cttttaaggg
     1861 ccttgctatt ttattcgatc ataccagcga gagctacaat gttggggact tgtttatcta
     1921 gctgcgtctg attaaccttt attatttttc ctgaaacagt tttatttcct gcagttaatg
     1981 tcgcgattgg aatacctaag gctccagata cgacagatgc tgtgtttgca tcatttactt
     2041 catactgaaa catattcctg aaagatcttt tattatttgg cgttgagctc ccatttgggt
     2101 gaatacaaaa cagacttggt tttgtttgcc atactttcca aatttcatca ccaatttcgg
     2161 ttactaccgt cttaaatgca ctggcagatg aattgttcat ttgcgttaat ctatttccaa
     2221 catacatata tatttgtggt agcgacatag aataacgatt gctattaaga tagaaatctg
     2281 attgtggttc attctgatgg cgacgctcac cataaactaa agaaagtaca gccttaactg
     2341 ctctctttga tgaaccatcg gcagagaaag gaattaaaag gcgctctgct gcggttaaag
     2401 ctaactctgt ataaatagag aagctagggt tgcagtcaat gaatacgcag ctatcttcat
     2461 tattccatga gttgcaaatg tcactgatta aatcattaat ccagaggtga acagtacgcc
     2521 atgcatcatg gggacctgga aatgatgccc caacaactct tgatgcttgg agttctaatt
     2581 gttcatcgcc agtgacaaga tatagattat ttggaacttt actattgtgt gtagatacat
     2641 ttattacata gctagaacca gtgttggtag gaatatatgg acttctgatc ctatcctcaa
     2701 tatatccaga tattgtcttt ctctgtattg ctgaatgaat ctggtttagt gtgttttcgc
     2761 catttatcat tccaccaagt aacatagagg acgagttcgc ttgtgggcac aggtcaacca
     2821 ccaggacctt tttagtaggg ttctgtctgg catattcaga ggctaactga aaagtaaggt
     2881 aacttttccc tacaccaccc ttgttattcc atacggcata gatcgccatc tataactcct
     2941 aatctagttt tgttgttagc ttagattcag ggtactagag attattgttc tactgttagt
     3001 ttctttcagc tgactgcctt tttttggaac gatattcagt cgaaggggtt ggctggctca
     3061 ccatgactta tctggagttg tcgaattccg attaaaagga tcttcttgag atcctaacct
     3121 gcgggatgta atcttttgct ctgtaaacga aaaaaccacc tggggaggtg gtttttcgaa
     3181 ggatcagtaa gttggggaac ttctgaaccg tggtaacagg gtgtacaaga ccgctgccac
     3241 caaatctgtc ctttcagttt agctgtagtt aggctgtaac ttcaagaact ctgcaccagt
     3301 aattcttgta caccccttac cagtggctgc tgccagtggc gttttgtcgt gccttccggg
     3361 ttggactcaa gtcgaaagtt accggaacag gcgcagcagt cggactgaac ggggggttcg
     3421 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     3481 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     3541 gcgcacgagg gagccatcag ggggaaacgc ctgatatctt tatagtcctg tcgggtttcg
     3601 ccaccactga tttgagcgtc cgtttctgtg atgcttgtca ggggggcgga gcctatggaa
     3661 aaacggccgc tgtgcggcct tcccttttct aaattacgtg atcgctcggt tttctcgagc
     3721 tttcatacgc atgctcctgc cgctcgccgc agccgaacgg caggagcgta gcgactgagt
     3781 gagcgaggaa gcggaaaaga gaacatccct acactgagca ctgacgctct gaggcctctt
     3841 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagca tcaatctctg
     3901 ctcctgctgg agaagcttaa cgagcttgat cttgatgtcg aggccgacct ttgtgagaaa
     3961 ctccacgatg atggggtcgg ttccggctga gggcgaaatg acaccctaag ctttcggttc
     4021 cttgggccaa agatattcgc cagtcagtag aatgtgcgcc cagcccaatg gggatatgtg
     4081 gggaagaaat tcagggggaa catccaaccc ttcgttccgc cgctccgtga cggcatgacc
     4141 aagatggacg gtattccagt aaatgatcac cgcagtcaat aaattgagcc cagcgattcg
     4201 gtagtgctgc ccctctgtcg tgcgatcgcg aatttccccc tgcctcccga tacggagcgc
     4261 atttttgagc gcatggtggg cctctccctt gttaagaccg atctgagcac gccgctgcat
     4321 gtccgtatcc aggatccact caataatgaa aagggtccgt tcaatacgac caacttcacg
     4381 aagcgcaact gcaaggttgt tttgtcgtgg gtaagaagcg agcttgcgca ggagttggct
     4441 gggcctgatt ttgccagcgg tcatcgtcgc ggcacaacgg aaaatatcag gccagttcgc
     4501 aacgataaga tcctcccggg cttttccacc taccaacttg cgtaactccc tgggggtcgt
     4561 atcgggatta aatacgtaca accgcttcga tggcagatcc ctgattcgca gaacgagatt
     4621 gtagccgagc aggctactgg ctccgaacaa atggtcggtg aatcctgctg tatcggcata
     4681 ctgttcgcga acatggcgac cgacctcgtt catcagtagt ccatcgagaa tatacggtgc
     4741 ctcgctcacg gtcgccggga tcgactgaca agcgaatggc gcgaactggt cgcttacgtg
     4801 agtatacgct ttgaggccgg gaacagaacc atatttggca ttgaccatgt tcatggcttc
     4861 gccatgccgc gctgtcggga aaaactgacc atcgctcgat gctgacgtgc ccatccccca
     4921 gacgcgtgac atcggcagtt taccctgcgc ggccaccaca attgccaatg cctggttcat
     4981 ggcttcgctt tcaacatgcc agcgggcaag gcgtgagagc tgccagtaat catgcgtgtt
     5041 tgtagcttcc gccatcttac gcaggcccag attgagccct tcagcgagca ggacgttgag
     5101 cagaccgatc cggtcgcgac atggagcccc ggttctcaga tgggtaaacg catctgtgaa
     5161 accaagggct gcatcaactt caagcagcat gtcggtaatc cgaacggacg gcattcggcg
     5221 atacagatcc agtatgagtg cctcggcacc atccggcacg tctgctgtca acctgtcgat
     5281 ccgcaacgtt ccatcttcta tgctaccgtg cggaatagtg ccgttacggg cagcccgggc
     5341 cagccgctta agagcgatcg tgagtcgcgc ctttctgtct gccagccaat cctgtgggtt
     5401 ggaaggcacg gccagttttg cattttcctg cgccgcgatc atcggcacca gtacctgctt
     5461 gaggtcacca tagcggcgcg aatgagcgag ccagacatct ccggaacgaa aagcatcccg
     5521 gaggtgaaag agtaccgcca cttcccaaag acgggtatct cctttttcct gagctcgtaa
     5581 atgacggttc catttggagc tgggccgcag gaaacgcctt tctggcgatg caacaccttt
     5641 catctctccg atcgacaaag ctgctgctac caatggtccg gcgaccggcg cggcttcgag
     5701 cttcagacag cgcaacatgc ggggcgcata acgacgaaag cgatggtatc cctgcccgac
     5761 atatgcaaga ggctcatcgg ctagcgtgtt gctgagttga gtccctgtcg ctaccagttg
     5821 agcgagccgg tcccatgcaa ccgaactggc gacagccatc tccagcgggg ttccgtcact
     5881 gcgggcctca agcaacgaag ctcccagcgc ggtgaaggta cggatcgtat ccgtgagtgt
     5941 ggctttagag ccggaaattg tttcgtcatg ctggcgcttc gcttcccgcc aggtttttcc
     6001 tacgatcctg tcatgggttt cgactatggc atcagcaatc gccgcttccc actccacaac
     6061 acagacggca aggatcgccc agcggcggtc cgaagtgatg tcacgcaaac cgtcggtgaa
     6121 gtagcgttca ccctgccgac gcagccgggc aatgcgatgg gcaggtatgc tggccaaagc
     6181 actatgattg atattcaggg tacgcagaaa ttcgagcctg tcgagcaaac ggttagcagc
     6241 agccgagttg ttaccaacct cgaagttgcg aagccagatg aaacgactga tattgccggc
     6301 gagcatttca ctcagaagtt tgtccaggtg atcgcgaaca tccgctgtta aattttccgc
     6361 aatccgcgtt tcaatccgcc gctcagcggc gaccagagca tccgcgcaca agcgctcgat
     6421 tgtcgatact gcgggcagaa tggtggaagt ttcccgacac cgcacaataa aacgatgagc
     6481 aagatcctcg tttgatctgg catcttcggc ctggccgaaa gtccactccc gcagatcacg
     6541 ggcaccacgg cccgtgaagg tcttgtagcc gtaaatttcg cgcagcgtgt ccatgtgctg
     6601 ctgacgggtt tggcgccgtg tggcataagt gagaagcgca tcagccggaa ctccaagctg
     6661 agcaccgacg aaggaaagga cttcacgcgg gatcatctca ccaggagcca gtgcacggcc
     6721 cggatatcgt aaggcacaaa gttgcagggc aaagccaatc ctgttttccg gtctgcggcg
     6781 ctgcctaatg ttttccaggt catcatcgcc cagcgtgtag aacttcagta gcgacagttc
     6841 gtccgtgggc agatcgaaca gcgctgctcg ctgccgttcg gtgaaaatat ggcgtcgtga
     6901 catacaaatt cgtccctttt gaagtatagt ctgttttgga caacagccag cccatataaa
     6961 tcagggcgtt ccgatacaaa aatccaggag ggttcaattg ggacatcgtg ccgccattta
     7021 ctgccgggtt tcaacagcgg atcagtcttg tgaacgccag gaatttgatc tgcgagcctt
     7081 cgccggccgt gccggctacg acgtggtggg aatatttaag gaaacaggtt caggaactaa
     7141 actcgaccgg gccgagcgaa agaaagtcct ggcgcttgcc cagtccagac aaattgatgc
     7201 aatcctggtc actgagcttt cccggtgggg gcgctcgacg ctcgatctgc tcaatacgct
     7261 acgtgaactg gagaactgga aggtttccgt gatagccatg aatggaatgg cgttcgatct
     7321 ttcgtcgccg tatggacgaa tgctggcgac gtttctttcc ggcattgcgg agtttgagcg
     7381 ggatctcatc agcgagcggg tcaagtcagg ccttgctgtt gcgaaggcac gtggtaagag
     7441 gcttggtcgt caggccggag tgcgaccaaa atcagaccga cttttgccta aggtggttgc
     7501 gatgagggcc gagggacgca gctatcgctg gatcgcacgc gagctcggta tcagcaagaa
     7561 taccgtcgct gacatcgtgc aacgacacag agctaacgct tagggtgtca tttcgccctc
     7621 agccggaacc gaccccatga tgcggagcat cttttccgta cactgtcctc gagactggat
     7681 gcattacagg atggtaactg atggccagag tgtctattag tgaggcagcc cgactgacgg
     7741 gtaagagtag aacaacactg cacaggttaa ttaaagcagg tgacctgtcc acctgtagcg
     7801 gtgagcgcaa tgcaaaaatg gtagatactt cagagctttt acgtgtcttt ggtccctttg
     7861 aacaactgaa gggtgaacac cattctggac aggtctctga acagcgcgat acagcctctg
     7921 ttgaccagtg tgaacaggtg attcgtcatc tgaaacagga gattgaacac ctgaacactt
     7981 tacttttgtc caaagattcg cacattgaca gcctgaaaca ggccatgctc cttcttgaaa
     8041 atcaccaggc aaaattaact gaatccccgg cgccatggtg gaaattctgg aaaaaatcct
     8101 gaccctgtca tgcttcagtt ttcctctccg gccatcccgg ggagttcctg aaccttccca
     8161 aaaatagtca gatctctgaa cgctcctcac cactaaaaat acccacaccc cacgcaaaat
     8221 taaaattttg cagatttgta tttaatatca gtagtttact ttcctgagat ttcgtttttt
     8281 aaattttatt tatatagatc cgactctatg acgactcgtc atagagtccc cggcgagtcg
     8341 tcatagagtc ggtaacgact cagggctata aaaacaatga gttaataacg acccgactct
     8401 agacccgact ccccgtttgg ttgtttttta gtcgcggcga ctctaggtac gactctagtg
     8461 cagagtcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac gtgattgccg
     8521 gccgtcgacg aacccgattc ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa
     8581 cgcttcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc
     8641 cggaaacagg acttaccctg
//
