LOCUS       NZ_CP102556             9057 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KPH3 plasmid p4, complete sequence.
ACCESSION   NZ_CP102556
VERSION     NZ_CP102556.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30176386
            Assembly: GCF_024666505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPH3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:16:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,472
            CDSs (total)                      :: 5,350
            Genes (coding)                    :: 5,243
            CDSs (with protein)               :: 5,243
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 66 of 107
            Pseudo Genes (internal stop)      :: 10 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102556.1.
            Bacteria and source DNA available from Human.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPH3"
                     /isolation_source="microbial"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="15.8700 N 100.9925 E"
                     /collection_date="2020"
                     /collected_by="Faculty of Medicine, Chulalongkorn
                     University"
     gene            complement(415..816)
                     /locus_tag="NUG48_RS27200"
                     /old_locus_tag="NUG48_27205"
     CDS             complement(415..816)
                     /locus_tag="NUG48_RS27200"
                     /old_locus_tag="NUG48_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_039817125.1"
                     /translation="MARVSISEASRLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKSEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="transcriptional regulator"
     gene            complement(816..1007)
                     /locus_tag="NUG48_RS27205"
                     /old_locus_tag="NUG48_27210"
     CDS             complement(816..1007)
                     /locus_tag="NUG48_RS27205"
                     /old_locus_tag="NUG48_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1416..1529
                     /locus_tag="NUG48_RS27370"
     CDS             1416..1529
                     /locus_tag="NUG48_RS27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072016307.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPVRHAKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            1887..2198
                     /locus_tag="NUG48_RS27210"
                     /old_locus_tag="NUG48_27215"
     CDS             1887..2198
                     /locus_tag="NUG48_RS27210"
                     /old_locus_tag="NUG48_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134632.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_039817123.1"
                     /translation="MSNQNIFEILTSDPVSLNMWNLKSNLILAIHKTVRDNGWNQTEAA
                     AHLGISQPRVSNMLKGKLDKFSTDSLLEIMFKLGYKLDMQVHPSSEVILEMGMKKAML"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(2218..2553)
                     /locus_tag="NUG48_RS27215"
                     /old_locus_tag="NUG48_27220"
     CDS             complement(2218..2553)
                     /locus_tag="NUG48_RS27215"
                     /old_locus_tag="NUG48_27220"
                     /inference="COORDINATES: protein motif:HMM:NF017757.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_077255464.1"
                     /translation="MSASEMKEICLDVTGVDKELKSLPIDKALAFLHNLKLVAAGIEPE
                     LKVEHLKVVAPGVIELKINGSPAYRCVYTIKSPGKVIVLRVFKKTTNGPARKDLEVVKQ
                     RLKNLKI"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3206..4326)
                     /locus_tag="NUG48_RS27220"
                     /old_locus_tag="NUG48_27225"
     CDS             complement(join(3206..4068,4068..4326))
                     /locus_tag="NUG48_RS27220"
                     /old_locus_tag="NUG48_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_087759376.1"
                     /translation="MKKRFSDEQIICILREAEAGVPARELCRKHAISDATFYTWRKKYG
                     GMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCDAT
                     GLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRREGL
                     HVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALSTGRRI
                     KCLTCVDDFTKECLTVTVAFGISGVQVSRILDSIALFRGYPATIRTDQGPEFTCRALDQ
                     WAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSNIVHARKIINDWRQDYNE
                     CRPHSALNYQTPSEFAARWRNGKCEGKQTDLTN"
                     /name="IS3 family transposase"
     gene            <4385..4516
                     /locus_tag="NUG48_RS27225"
                     /old_locus_tag="NUG48_27230"
                     /pseudo=""
     CDS             <4385..4516
                     /locus_tag="NUG48_RS27225"
                     /old_locus_tag="NUG48_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /name="protein L"
     gene            4607..4906
                     /locus_tag="NUG48_RS27230"
                     /old_locus_tag="NUG48_27235"
     CDS             4607..4906
                     /locus_tag="NUG48_RS27230"
                     /old_locus_tag="NUG48_27235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125564845.1"
                     /translation="MSNPPFEFVIRYHDLSPEQVRSFIDDAFHRVDLVLEKQLSDRPGF
                     FIPTCKSLTTNPSLSEIAIAVYNTYAVTEMIKDQLLRQSSNITLIKDIREIFVS"
                     /name="hypothetical protein"
     gene            complement(5427..6032)
                     /locus_tag="NUG48_RS27235"
                     /old_locus_tag="NUG48_27240"
     CDS             complement(5427..6032)
                     /locus_tag="NUG48_RS27235"
                     /old_locus_tag="NUG48_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            6127..9024
                     /locus_tag="NUG48_RS27240"
                     /old_locus_tag="NUG48_27245"
     CDS             6127..9024
                     /locus_tag="NUG48_RS27240"
                     /old_locus_tag="NUG48_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     Typing          1056..1529
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.627"
                     /coverage="92.0"
                     /evalue="9.83e-170"
ORIGIN
        1 aatcacgtta tggcaagtcg ccggacgact caccacacga ttcaccggcg actcttcact
       61 agagtcgtac ccagagtcgt cacgtctaaa aaacaaccaa acggggagtc gggtctagag
      121 tcgggtcgtt gttaacttat tgtttttata gtcctgagtc gttaccgact ctatgacgac
      181 tcgccgggga ctctatgacg actcgtcata gagtcggatc tgtataaata aaattaaaaa
      241 aacgaaattt atagaacgta agctgctgat ataaaataca aatctgcaaa attttaattt
      301 tgcgtggggt gtggatattt ttagtggtga ggagcgttca gagatctggc gatttttggg
      361 aaggttcagg aactccccgg gatggccgga gaggaaaact gaagcatgac ggggtcagga
      421 ttttttccag aatttccacc atggcgccgg ggattcagtt aattttgcct ggtgattttc
      481 aagaaggagc atggcctgtt tcaggctatc aatgtgcgaa tccttggaca aaagtaaggt
      541 gttcaggtgt tcaatctcct gtttcagatg acgaatcacc tgttcactct ggtcagcaga
      601 ggctgtatca cgctgttcag agacctgtcc agaatggtgt tcactcttcg gttgctcaaa
      661 gggaccaaag acacgtaaaa gctctgaagt atctaccatt tttgcattac gttcaccgct
      721 acaggtggac aggtcacctg ctttaattaa cctgtgcagt gttgttctac tcttacccgt
      781 cagtcgggaa gcctcactaa tagacactcg ggccatcagt taccatcctg taatgcatcc
      841 agtctcgagg acagtgtacg gaaaagatgc tccgcatcat cgtggagttt ctcacaaagg
      901 tcagcctcag catcgagatc aagctcgtta agcttctcca gcaggagcag ggactgattc
      961 tggatgaact tagccatgtt cagcgccgtt ttctgttgtt tcgtcataaa gggcctcaga
     1021 gcgtcagtgc tcaatgtaaa accggtctct tttccgcttc ctcgctcact cagtcgctac
     1081 gctcctaccg ttcggctgcg gcgagcggtc gcaacgcaca cataagggca taggacagac
     1141 agctcggaaa ggacaacgga aggccgcggc gttgccgttt ttccataggc tccgcccccc
     1201 tgataagcat cacaaaaaca gacgctcaaa tcagtggtgg cgaaacccga caggactcta
     1261 aagataccag gcgtttcccc ctgatggctc cctcgtgcgc tctcctgttc ctgcctttcg
     1321 gtttaccggt gtcattccgc agttatggcc gcgtttatct cattccacgc ctgacactca
     1381 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     1441 actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggtaaga catgcaaaag
     1501 cgccactggc agcagccatt ggtaactgat tagaggagtg aagtcttgaa aagacacgta
     1561 ctatacggct acactgataa aacagatttg gtgactgtgc tcctctaagc cagttacctc
     1621 ggttcataaa cttgccggtt tgatgaacct tgaaaaaccg cctccccagg cggtttttct
     1681 ttttaaaaca aatgaagact acgctgacga ccaagatcca gtcaacaagt gttgttagaa
     1741 tgctttaaat ttacaaataa aaccccatct acatcttgtc atgcatactg taacttgtat
     1801 aatccgcaaa ctttagtgta agctaataat cgttcctatt gtcttgtgac aactaaatgc
     1861 tcgcattcag ataggagatg aaaattatga gcaatcaaaa catattcgaa atactaacga
     1921 gtgatccggt atccctaaac atgtggaacc tgaaatcaaa cctgatcctc gccatacata
     1981 agacggtaag agataacggc tggaaccaaa cagaggcggc cgcacatcta ggcataagcc
     2041 aacctagggt tagcaacatg ctgaaaggga agctggacaa gttttctaca gattcactac
     2101 tggaaatcat gttcaagctg ggttacaaat tagacatgca agtacaccca tcctctgaag
     2161 taattttaga gatgggcatg aaaaaggcga tgctgtgaac atcgccttga gtggatacta
     2221 tattttgaga tttttcagcc gttgcttcac gacttctaag tctttgcgtg caggtccgtt
     2281 agtcgttttc ttgaagacac gtaacacgat caccttgccg ggtgatttta tcgtgtacac
     2341 acaacgataa gcagggctac catttatctt caactcaatc acgcctggag caactacttt
     2401 caggtgttca actttcagtt ctggttcaat tccagcggca accaacttca agttgtgcaa
     2461 aaaagctaaa gctttatcaa taggtaaaga ctttagctct ttatcaacac cagttacgtc
     2521 tagacaaatc tctttcattt cgcttgcgga catagtgtcc tcctttgttg gggtttagtg
     2581 gtcgaacaat taaatttctc atttgatact ccgattaaaa aaacctcact aaaaaaccaa
     2641 acctctacgc ctgatgagat tcaccaggta gaggcattta atttcagtgg ttcaggacac
     2701 tgctttaggc aatatccatt gcatcaatat actctaagtt gtataaacta acaatatgcc
     2761 gcttcgcttg cgcatgccgt tagctcaata aataagctat cagctgttta cgaaagtaat
     2821 cctccatcga agctaaaagt ctccgtaaag catcacaact gaaagaagga gttaagagat
     2881 ggcagataga agaccgctaa acaaagatgt ggtagtgcca aagcctggtg gcggaaccca
     2941 gcccagagac agaaacaatg acggaacggt gcgcaaaaaa cgctcagacg caggaaaacc
     3001 aagaaaataa acgataagaa aggtaaaaga atggctgcat atacggaaaa aacagagcag
     3061 ttcttacagc gagttacaca cacacagact tggtggacat caacatacga tccaagcgat
     3121 acagttccag tatcaggcat ttatcgctgt accgtatgcg gtaaagagat aactagtgac
     3181 ctgcccccag agttagatac aaccgtcagt tagtaaggtc ggtttgttta ccttcacatt
     3241 ttccatttcg ccaccgtgct gcaaactctg atggcgtctg ataattcagt gctgaatgtg
     3301 gacgacactc gttataatcc tgccgccagt cattaatgat tttccttgcg tgaacgatat
     3361 tgctgaacca gtgctcattc aggcattcat cgcgaaatcg tccgttaaag ctctcaataa
     3421 atccgttctg cgttggcttg cccggctgga ttaagcgcaa ctcaacacca tgctcaaagg
     3481 cccattgatc cagtgcacgg caagtgaact ccggcccctg gtcagttctt atcgtcgccg
     3541 gatagcctcg aaacagtgca atgctgtcca gaatacgcga gacctgaacg cctgaaatcc
     3601 caaaggcaac agtgaccgtc aggcattcct ttgtgaaatc atcgacgcag gtaagacact
     3661 tgatcctgcg accggtggaa agtgcgtcca tgacgaaatc catcgaccag gtcagattgg
     3721 gcgccgccgg acggagcagc ggcagacgtt ctgttgccag ccctttacga cgtcttctgc
     3781 gttttacgcc caggccactg aggtgataaa gccggtacac gcgcttatga ttaacatgaa
     3841 gcccttcacg gcgcagcaac tgccaaatac gacggtagcc aaaacgcctg cgctccagtg
     3901 ccagctcagt gatgcgccct gataaatgcg catcagcagc cggacggtga gcctcatagc
     3961 ggcaggtcga cagggataaa cctgtaagcc tgcaggcacg acgttgcgac agaccggtcg
     4021 catcacacat cagcatcacg gcttcccgct tctggtctgt cgtcagtact ttcgcccaag
     4081 agccacctga agcgcctctt tatccagcat ggcttcggca agcagcttct tgagtctggc
     4141 gttctcttcc tcaagcgact tcaggcgctt aacttcaggc acctccatac cgccatactt
     4201 cttacgccag gtgtaaaacg tggcatcgga aatggcatgc ttgcggcaga gttcacgggc
     4261 gggtacccca gcttcggctt cgcggagaat acagatgatc tgttcgtcgg aaaaacgctt
     4321 cttcatgggg atgtcctcat gtggcttatg aagacattac taacatcggg gtgtactaat
     4381 caacggggag caggtcacta gtaataaaaa cgatcctttc cccccacaaa accatcatca
     4441 acatagtcaa aaacaaaatg ttaaatggca gctaattgtg aggacagaca caaacggtga
     4501 taaatttggt aactgagaca cggaagccct tcggggcttt ccctcagtga tttaccagaa
     4561 agtctcatta ctcactcgta ccaaacaatc cacgatctga ggtcttatgt ctaatccacc
     4621 ttttgaattt gtcattcgtt atcatgacct gtctccagag caagtgcgga gctttattga
     4681 cgatgctttt catcgtgttg atctcgttct agaaaaacag ttatccgatc gtccaggctt
     4741 ttttattcct acatgtaagt cactaactac taatccgtct ttatctgaaa tagctatcgc
     4801 tgtttacaac acatatgccg ttactgaaat gataaaggac caattgcttc gacaatcttc
     4861 caatatcact ctcattaaag acataagaga gatctttgtc agctaggatc ttctctacca
     4921 caccacaagt gcttatctca taacagctta tttggccaaa caagactggc gcagagggag
     4981 tttccgaaca ggtcgccggg aggtggaccg cttcgcacct cagcaggaaa tccatcagac
     5041 caatcggggc tttttacatc tatcatttaa cataaaggta tgattgtacg caccgctaaa
     5101 acggcctcag ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac
     5161 ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg agtatgcacg caagtgcata
     5221 gcgcgaaaat tcacgccatt cagccagggt aagtcctgtt tccggcgttc ttcccatccg
     5281 aaaacggtga acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg
     5341 aaaaccggtt ttcagcacgt tcaggaatcg ggttcgtcga cggccggcaa tcaggggtcg
     5401 gttccggctg agggcgaaat gacaccctaa gcgttagctc tgtgtcgttg cacgatgtca
     5461 gcgacggtat tcttgctgat accgagctcg cgtgcgatcc agcgatagct gcgtccctcg
     5521 gccctcatcg caaccacctt aggcaaaagt cggtctgatt ttggtcgcac tccggcctga
     5581 cgaccaagcc tcttaccacg tgccttcgca acagcaaggc ctgacttgac ccgctcgctg
     5641 atgagatccc gctcaaactc cgcaatgccg gaaagaaacg tcgccagcat tcgtccatac
     5701 ggcgacgaaa gatcgaacgc cattccattc atggctatca cggaaacctt ccagttctcc
     5761 agttcacgta gcgtattgag cagatcgagc gtcgagcgcc cccaccggga aagctcagtg
     5821 accaggattg catcaatttg tctggactgg gcaagcgcca ggactttctt tcgctcggcc
     5881 cggtcgagtt tagttcctga acctgtttcc ttaaatattc ccaccacgtc gtagccggca
     5941 cggccggcga aggctcgcag atcaaattcc tggcgttcac aagactgatc cgctgttgaa
     6001 acccggcagt aaatggcggc acgatgtccc aattgaaccc tcctggattt ttgtatcgga
     6061 acgccctgat ttatatgggc tggctgttgt ccaaaacaga ctatacttca aaagggacga
     6121 atttgtatgt cacgacgcca tattttcacc gaacggcagc gagcagcgct gttcgatctg
     6181 cccacggacg aactgtcgct actgaagttc tacacgctgg gcgatgatga cctggaaaac
     6241 attaggcagc gccgcagacc ggaaaacagg attggctttg ccctgcaact ttgtgcctta
     6301 cgatatccgg gccgtgcact ggctcctggt gagatgatcc cgcgtgaagt cctttccttc
     6361 gtcggtgctc agcttggagt tccggctgat gcgcttctca cttatgccac acggcgccaa
     6421 acccgtcagc agcacatgga cacgctgcgc gaaatttacg gctacaagac cttcacgggc
     6481 cgtggtgccc gtgatctgcg ggagtggact ttcggccagg ccgaagatgc cagatcaaac
     6541 gaggatcttg ctcatcgttt tattgtgcgg tgtcgggaaa cttccaccat tctgcccgca
     6601 gtatcgacaa tcgagcgctt gtgcgcggat gctctggtcg ccgctgagcg gcggattgaa
     6661 acgcggattg cggaaaattt aacagcggat gttcgcgatc acctggacaa acttctgagt
     6721 gaaatgctcg ccggcaatat cagtcgtttc atctggcttc gcaacttcga ggttggtaac
     6781 aactcggctg ctgctaaccg tttgctcgac aggctcgaat ttctgcgtac cctgaatatc
     6841 aatcatagtg ctttggccag catacctgcc catcgcattg cccggctgcg tcggcagggt
     6901 gaacgctact tcaccgacgg tttgcgtgac atcacttcgg accgccgctg ggcgatcctt
     6961 gccgtctgtg ttgtggagtg ggaagcggcg attgctgatg ccatagtcga aacccatgac
     7021 aggatcgtag gaaaaacctg gcgggaagcg aagcgccagc atgacgaaac aatttccggc
     7081 tctaaagcca cactcacgga tacgatccgt accttcaccg cgctgggagc ttcgttgctt
     7141 gaggcccgca gtgacggaac cccgctggag atggctgtcg ccagttcggt tgcatgggac
     7201 cggctcgctc aactggtagc gacagggact caactcagca acacgctagc cgatgagcct
     7261 cttgcatatg tcgggcaggg ataccatcgc tttcgtcgtt atgcgccccg catgttgcgc
     7321 tgtctgaagc tcgaagccgc gccggtcgcc ggaccattgg tagcagcagc tttgtcgatc
     7381 ggagagatga aaggtgttgc atcgccagaa aggcgtttcc tgcggcccag ctccaaatgg
     7441 aaccgtcatt tacgagctca ggaaaaagga gatacccgtc tttgggaagt ggcggtactc
     7501 tttcacctcc gggatgcttt tcgttccgga gatgtctggc tcgctcattc gcgccgctat
     7561 ggtgacctca agcaggtact ggtgccgatg atcgcggcgc aggaaaatgc aaaactggcc
     7621 gtgccttcca acccacagga ttggctggca gacagaaagg cgcgactcac gatcgctctt
     7681 aagcggctgg cccgggctgc ccgtaacggc actattccgc acggtagcat agaagatgga
     7741 acgttgcgga tcgacaggtt gacagcagac gtgccggatg gtgccgaggc actcatactg
     7801 gatctgtatc gccgaatgcc gtccgttcgg attaccgaca tgctgcttga agttgatgca
     7861 gcccttggtt tcacagatgc gtttacccat ctgagaaccg gggctccatg tcgcgaccgg
     7921 atcggtctgc tcaacgtcct gctcgctgaa gggctcaatc tgggcctgcg taagatggcg
     7981 gaagctacaa acacgcatga ttactggcag ctctcacgcc ttgcccgctg gcatgttgaa
     8041 agcgaagcca tgaaccaggc attggcaatt gtggtggccg cgcagggtaa actgccgatg
     8101 tcacgcgtct gggggatggg cacgtcagca tcgagcgatg gtcagttttt cccgacagcg
     8161 cggcatggcg aagccatgaa catggtcaat gccaaatatg gttctgttcc cggcctcaaa
     8221 gcgtatactc acgtaagcga ccagttcgcg ccattcgctt gtcagtcgat cccggcgacc
     8281 gtgagcgagg caccgtatat tctcgatgga ctactgatga acgaggtcgg tcgccatgtt
     8341 cgcgaacagt atgccgatac agcaggattc accgaccatt tgttcggagc cagtagcctg
     8401 ctcggctaca atctcgttct gcgaatcagg gatctgccat cgaagcggtt gtacgtattt
     8461 aatcccgata cgacccccag ggagttacgc aagttggtag gtggaaaagc ccgggaggat
     8521 cttatcgttg cgaactggcc tgatattttc cgttgtgccg cgacgatgac cgctggcaaa
     8581 atcaggccca gccaactcct gcgcaagctc gcttcttacc cacgacaaaa caaccttgca
     8641 gttgcgcttc gtgaagttgg tcgtattgaa cggacccttt tcattattga gtggatcctg
     8701 gatacggaca tgcagcggcg tgctcagatc ggtcttaaca agggagaggc ccaccatgcg
     8761 ctcaaaaatg cgctccgtat cgggaggcag ggggaaattc gcgatcgcac gacagagggg
     8821 cagcactacc gaatcgctgg gctcaattta ttgactgcgg tgatcattta ctggaatacc
     8881 gtccatcttg gtcatgccgt cacggagcgg cggaacgaag ggttggatgt tccccctgaa
     8941 tttcttcccc acatatcccc attgggctgg gcgcacattc tactgactgg cgaatatctt
     9001 tggcccaagg aaccgaaagc ttagggtgtc atttcgccct cagccggaac cgacccc
//
