LOCUS       NZ_CP102551             4642 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KPH1 plasmid p5, complete sequence.
ACCESSION   NZ_CP102551
VERSION     NZ_CP102551.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30176384
            Assembly: GCF_024666265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPH1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:17:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,605
            CDSs (total)                      :: 5,478
            Genes (coding)                    :: 5,324
            CDSs (with protein)               :: 5,324
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 72 of 154
            Pseudo Genes (incomplete)         :: 82 of 154
            Pseudo Genes (internal stop)      :: 16 of 154
            Pseudo Genes (multiple problems)  :: 16 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102551.1.
            Bacteria and source DNA available from Human.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPH1"
                     /isolation_source="microbial"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="15.8700 N 100.9925 E"
                     /collection_date="2020"
                     /collected_by="Faculty of Medicine, Chulalongkorn
                     University"
     gene            120..329
                     /locus_tag="NUG47_RS27945"
                     /old_locus_tag="NUG47_27945"
     CDS             120..329
                     /locus_tag="NUG47_RS27945"
                     /old_locus_tag="NUG47_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(1172..1573)
                     /locus_tag="NUG47_RS27950"
                     /old_locus_tag="NUG47_27950"
     CDS             complement(1172..1573)
                     /locus_tag="NUG47_RS27950"
                     /old_locus_tag="NUG47_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1573..1764)
                     /locus_tag="NUG47_RS27955"
                     /old_locus_tag="NUG47_27955"
     CDS             complement(1573..1764)
                     /locus_tag="NUG47_RS27955"
                     /old_locus_tag="NUG47_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2180..2293
                     /locus_tag="NUG47_RS28050"
     CDS             2180..2293
                     /locus_tag="NUG47_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080882241.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2497..4179)
                     /locus_tag="NUG47_RS27960"
                     /old_locus_tag="NUG47_27960"
     CDS             complement(2497..4179)
                     /locus_tag="NUG47_RS27960"
                     /old_locus_tag="NUG47_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697690.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_223862165.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(4192..4374)
                     /locus_tag="NUG47_RS27965"
                     /old_locus_tag="NUG47_27965"
     CDS             complement(4192..4374)
                     /locus_tag="NUG47_RS27965"
                     /old_locus_tag="NUG47_27965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     Typing          1886..2293
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttttttgaa tgatatccgc aaattgtttc gattttaatc aactaatggt cgtcctatca
       61 tttatcacat cagggcaaca acgccccata cagaacaaaa tcacttaagg aatacgacca
      121 tgaaatccat caaaactttc gttgcagttg ccgctctatc catgatgtct ttcggtgctt
      181 ttgctcagag cgtcagcgct accgcctcca ccctggatcg tgctgaagca aaaattgccg
      241 cacaggccgc tgaacaaggt gcgtcataca aaatcaccag cgctcagttc aataaccgtg
      301 tccacatgac ggctgaactg accaaataag atggtcgcaa agggagctgc cgaacaggcc
      361 gccgggaggc ggcccgtttc gtcccttagc aggaaaacct tcaggcccct tctggggctt
      421 ttttgcatct gttatttaac ataaaggcat gattgtacgc accgctaaaa cggcctcagg
      481 gcgtttctga cggaaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac
      541 gcgcaggctc tgtttttacg gcatgcatga gtatgcacgc aagtgcatag cgcgaaaatt
      601 cacgccattc agccagagta agtcctgttt ccggcgttct ttccatccga aaacggtgaa
      661 cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt
      721 tcagcacgtt caggaatcgg gttcgtcgac ggccggcaat cacgttatgg caagtcgccg
      781 gacgactcac cacacgattc accggcgact cttcactaga gtcgtaccca gagtcgccgc
      841 gtctaaaaaa caaccaaacg gggagtcgtg tctagagtcg ggtcgttatt aacttattgt
      901 ttttatagtc ctgagtcgtt accgactcta tgacgactcg ccggggactc tatgacgagt
      961 cgtcatagag tcggatctat ataaataaaa tttaaaaaac gaaatttcag gaaagtaacc
     1021 cactgatatt aaatacaaat ctgcaaaata ttaattttgc gtggggtgtg ggtattttta
     1081 gtggtgagga gcgttcagag atctgacgat ttttgggaag gttcaggaac tccccgggat
     1141 ggccggagag gaaaactgaa gcatgacggg gtcaggattt tttccagaat ttccaccatg
     1201 gcgccgggga ttcagttaat tttgcctggt gattttcgag aaggagcatg gcctgtttca
     1261 ggctgtcaat gtgcgaatcc ttggacaaaa gtaaggtatt caggtgttca atctcctgtt
     1321 tcagatgacg aatcacctgt tcactctggt cagcagaggc tgtatcacgc tgttcagaga
     1381 cctgtccaga atggtgttca cccttcggtt gctcaaaggg accaaagaca cgtaaaagct
     1441 ctgaagtatc taccattttt gcattgcgtt caccgctaca ggtggacagg tcacctgctt
     1501 taatcaacct gtgcagtgtt gttctactct tacccgtcag tcgggcagcc tcactaatag
     1561 acactctggc catcagttac catcctgtaa ttcatccagt ctcgaggaca gtgtacggaa
     1621 aagatgctcc gcatcatcgt ggagtttctc acaaaggtcg gcctcaacat cgagatcaag
     1681 ctcgttaagc ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag
     1741 cgctgttttc tgttgtttcg tcataagaag cctcagagcg tcagtgctca gtgtaagaac
     1801 agtctctttt ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg
     1861 agcggcagga gcatgcgtgt aaaagctcga gaaaaccgag cggtctcgtg atggggaaaa
     1921 ggaaaggccg cacagcggcc gtttttccat aggctccgcc cccctgacga gcatcacgga
     1981 atctgacgct caaatcagtg gcggcgaaac ccgacaggac tataaagata ccaggcgttt
     2041 ccccctgatg gctccctcgt gcgctctcct gttcctgcca ttcggtttac cggtgtcatt
     2101 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     2161 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgtcttatc
     2221 cggtaactat catcttgagt ccaacccggt aagacatgca aaagcaccac tggcagcagc
     2281 caccggtaac tgatttagag gagttagtct tgaagttatg cgccagttaa ggctaaactg
     2341 aaagaacaag ttttggtgac tgcgctcctc taagccagtt accttggttc atagagccgc
     2401 aaactctaga accttaaaaa aaccgccccg caaaggcggt ttttttcttt ctcgtctcaa
     2461 ggttaagctc tgtataacat cagagtcaac agatcttcat ttcgagtctg gtggtactgg
     2521 tggtactggt ggtactggtg gtactggcgg tacgggcggt acgggcggta cgggcggtac
     2581 gggcggtacg ggctgggaag ataaattaat agtcgaatca gttctcccga catgattgcc
     2641 agaatcaata tttgtgttag ttctaaaaat atcaacgact cgattttcga aatctaaata
     2701 gctatcaata aactgtatat caatgtgatt tttatattta gtggagtact tttctgcgtt
     2761 accgaaacta tctttccttt catctgtaga aaagaagaat ttttgatacc tacgaggtga
     2821 tacaccttca aaggattcaa atataacctt cccatgctca ttcacctcgg ttatagcatc
     2881 atcatgcaat tctaaagcga ggcttttttc atatggctct atataaacaa cttttgttat
     2941 tccaacagcg acaatatgtc tcgcgcagtt atgacaagga aatgtggttg tatataaaac
     3001 tttatcagaa aaacctccat tacctagtct agctaatgat gtaatagcat ccatctctgc
     3061 atgaattgag cgagagtact ctataataga agatactggt gtttttttat atattacatc
     3121 agccagttgc tctgcttgat cttctgttaa tttaacacca agttcacaag cgctttccga
     3181 cataagtatt tgtgaaattt tgcttttaat ctttagtttt ctaacttcat tataacattt
     3241 accacctttg tatacacaac gatgatcatt gacaccatca tccgcactat aaagcccacc
     3301 attgaattta ggtacatcat tacgcccaac ggaaatcaga ttcccattaa catccaatat
     3361 agcagcacca acttgacgag aaaggcatgc agattgcaga gatgctgaat gagctgcata
     3421 catacctttt tcatacgtgg ttggagttaa accatttagc ccatgtatca agccaataaa
     3481 acgcttaacc ttagccttaa ggtgcgccct ttgtccttga ttattcttaa caaaataatc
     3541 agagtcaagt atagctttcg cagtctgctg accataatcg ctctctgatt ttctatcgtg
     3601 atgaataact atatcgactt ctgacttact cagcccttca tctctaagat tacgctttct
     3661 ttctacttca cttctaatta cacctagtaa ataaaaatta tgttggtaga ccatccttaa
     3721 aagttcaatt tcatcctgat gcttcaactg atcgaccaaa aaagcctttt tgtttttttt
     3781 acttgaggtg tcaatgcctt tttctatttt tatttcatgg attacagctt cagatagtat
     3841 atgtgggtaa tatttatttc ttaacgcatc accaaaatct tgcagcttat gatatctatc
     3901 aaacggagta acaatatcat cacgccattc tgaaaaaagt ttttgcataa gctcgctaac
     3961 tcttatgcga taaacatcat atcctagatc atctagttct tcttcagtca catcaacaat
     4021 tgactttatt cctgagccaa cagcaccgca aaatcctata acaagatcca tagactgtct
     4081 ggaggttatt tggtttatgc tatcccctcc agaggagtcc tcgttagcac tatgaatcgg
     4141 attacttctt ttttctctta actttctagc caggctcata aaagacctca cttaccattt
     4201 taatgatttt gaatcctcaa ttatcttcct tatagcgtcc tctcttcttt ttttaatcat
     4261 ttcaacttca aaatcgaaaa ctttcttttc ctttcccagc tcatgccctc tagaatcatt
     4321 aatatatcta gaattcttat cataaatttt atttagagat agctctttac ccatcacacc
     4381 ctcctgcaag acgcatggat tttactgtta tctttataac aggcaatagc caagaaacgt
     4441 aatcgataga gatttttttt tcttatccta aacattagga aattcggcaa gagaaaagca
     4501 agagagatta cgccaaacta accattattt ttcaatttgt taaaataaaa tcatcaatgt
     4561 taacatttct atccaccacc tcatttggta cctaaaatcc agagataaac ctctggtctt
     4621 gatgcgctca attcttgttc tt
//
