LOCUS       NZ_CP102550             5251 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KPH1 plasmid p4, complete sequence.
ACCESSION   NZ_CP102550
VERSION     NZ_CP102550.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30176384
            Assembly: GCF_024666265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPH1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:17:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,605
            CDSs (total)                      :: 5,478
            Genes (coding)                    :: 5,324
            CDSs (with protein)               :: 5,324
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 72 of 154
            Pseudo Genes (incomplete)         :: 82 of 154
            Pseudo Genes (internal stop)      :: 16 of 154
            Pseudo Genes (multiple problems)  :: 16 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102550.1.
            Bacteria and source DNA available from Human.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPH1"
                     /isolation_source="microbial"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="15.8700 N 100.9925 E"
                     /collection_date="2020"
                     /collected_by="Faculty of Medicine, Chulalongkorn
                     University"
     gene            join(5120..5251,1..189)
                     /locus_tag="NUG47_RS27915"
                     /old_locus_tag="NUG47_27915"
     CDS             join(5120..5251,1..189)
                     /locus_tag="NUG47_RS27915"
                     /old_locus_tag="NUG47_27915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            398..>829
                     /locus_tag="NUG47_RS27920"
                     /old_locus_tag="NUG47_27920"
                     /pseudo=""
     CDS             398..>829
                     /locus_tag="NUG47_RS27920"
                     /old_locus_tag="NUG47_27920"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            867..1352
                     /locus_tag="NUG47_RS27925"
                     /old_locus_tag="NUG47_27925"
     CDS             867..1352
                     /locus_tag="NUG47_RS27925"
                     /old_locus_tag="NUG47_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1356..1568
                     /locus_tag="NUG47_RS27930"
                     /old_locus_tag="NUG47_27930"
     CDS             1356..1568
                     /locus_tag="NUG47_RS27930"
                     /old_locus_tag="NUG47_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2201..3556
                     /locus_tag="NUG47_RS27935"
                     /old_locus_tag="NUG47_27935"
     CDS             2201..3556
                     /locus_tag="NUG47_RS27935"
                     /old_locus_tag="NUG47_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            3549..4169
                     /locus_tag="NUG47_RS27940"
                     /old_locus_tag="NUG47_27940"
     CDS             3549..4169
                     /locus_tag="NUG47_RS27940"
                     /old_locus_tag="NUG47_27940"
                     /inference="COORDINATES: protein motif:HMM:NF025084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(4322..4483)
                     /locus_tag="NUG47_RS28045"
     CDS             complement(4322..4483)
                     /locus_tag="NUG47_RS28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     Typing          4834..5006
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(4371..4887)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.462"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          180..1684
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5121..5251
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="4.72e-22"
ORIGIN
        1 atatcgccgg cgctgctgcg ccagcttgcc ggtatgggga acaacctgaa ccagatcgcc
       61 cggaaagtga atgccggtgg tgccggtcat gaccgggtgc agattgtggc cgcactgatg
      121 gcaatcgacg cgggactgga gcggctgcgg catgcggtgc tggaaaaagg gggggatgat
      181 gatcgttaaa tttcaccccc gggggcgcgg tggtggtgcc ggtccggtgg attacctgct
      241 gggaaaagac cggcagcgcg agggggcgac cgtgctgcag ggaaaaccgg aggaagtccg
      301 ggagcttatc gatgcctcgc cgtatgcgaa aaagtacacc tccggcgtgc tgtcgttcgc
      361 ggaggctgaa ctgccgcccg ggcagcggga gcagatcatg gccagctttg agcgggttct
      421 gatgcccggg ctcgataaag accagtacag cattctgtgg gtggagcaca ccgacaaggg
      481 gcggctggag ctgaatttcc tgatccccaa cactgaactg ctgaccggga aacgcctgca
      541 gccgtactac gatcgcgccg accggccgcg catcgatgcc tggcagaccg tggtgaacgg
      601 caggctgggg ctgcatgatc caaacgcccc ggagaaccga cggttactgg tcacgccgtc
      661 cgcactgccg gaaacgaagc tggaagccgc tcaggcgatt acacgtgggt tgctggcctt
      721 agcctcatcc ggggagctca aaacgcgcga ggacgtcaca ggggcgctca cagcggcagg
      781 ttttgaggtg gtccgcacca caaaaaacag tatcagcatt gccgacccgg acggggggcg
      841 aaacatccga ctgaagggag ccatttatga gcagtctttt agcgctggcg aaggacttgg
      901 agcagcaatc gaaagcgcag cagcagagta ccggcgagat gctgaaagcc gcattcagcg
      961 agcacgagca gtctgtaaaa gcggaactga gcgcaagcgc gaagagaatc agcgacgcca
     1021 tcagcgccca cgagcagggt atgaccgcgg cgatgcagtc gaaccggctg agcgtgctgc
     1081 gtatggtcgg ccggacgtgg ctgaccatca ccctggtctc ggtactgctg atcgccacga
     1141 gcgggagcat tctgtggtgg caggggcagc agatcaccgg gaattaccag actatccggg
     1201 cgcaggagcg gacgcaggcc atgctgtcgg agaagaacca cggcgtgcag ctgtcgctct
     1261 gcggggagaa gaaactgtcc tgcgtcaaag tgaacccgaa agcgggagct tacggggagg
     1321 aagggaactg gatggtactg gagaggaaat agcccatgac ggaactggaa aaacagttgc
     1381 tgaccgcatt agagcagcta catcaggact actcgcaaag gctggacgag tgggagagcg
     1441 ccttcgcgga atggcagaag atgtctggtc ttatgcaacg ggagaacgcg gcgctgaacg
     1501 agcgcgtcac gcgcttgagt cagcaggtgg cgaacttgag caggcagtta cagcgtttga
     1561 gccagtagtg cagcggcatg aaatggcggt ggccgccgaa cgggcatacg agcagaaaca
     1621 gcatgaaaaa gagctggcgg aaacccgttc ccggcagcgg agctatgacg gtccgacgct
     1681 gggctgactg cgcgcatcca gtgacaaacc ccaagaagaa gggctcataa atttcccgct
     1741 aaatacggtc gtgatgtttc catcacagcg tatacaacgg agggattatg agactgttta
     1801 acgtgattga gacggtgctg aattgcctgt acatggcgct ggagatctgg gactggctgg
     1861 agaagcattt tcctgttttt aaggcgttca tccggattct ggcctgaaat cagaccgtat
     1921 gcatctggct gcatgaccat gcagccgggt aaaatttatc tgtcacgaac ggggcggtgt
     1981 atcgggtggt ttgttgccgg ttttacctga ctgccgcccc gtttgtggcg aacctgtccg
     2041 gagcggtgcg ggcaacagat gttatggtaa atagcgtgta attataaata gcacaacgtt
     2101 ttttaaggaa gatgttgttc caaaattaaa acatatagga tatctttcct atgattgatt
     2161 tttcgctttt ttggcgaaaa atccccgtta ggaggtatgc atgtttgttt atttgactat
     2221 tgaaaatttt aggtcggtta aggaaaaatc gacattaaac ttgagcgcat ccggttctag
     2281 taaccattta acaactcata tctataaaaa tgatggaatg aatgttggaa cgctcatgag
     2341 tgctgggatt tatggtgcta atgcgtctgg taaatctaac gttctcatgg cttttgaagc
     2401 tataaaattt atcgcaacag aaagcggtga tttgaaagaa ggtaaacgga ttccctgcta
     2461 tgagccatat gctctttctt cggctacgaa tgaggctcct gtaaagtttg aggctgaatt
     2521 ttattctgtt gaggggaatc ggttcagtta tgaagttgct tttattaaaa atcggatttt
     2581 atttgaatca ttagactatt atccatctag ggttaaagca aatttattta ctagagatga
     2641 gagcgacacc tgggaaacaa tcaagtttgg tgggcattat aaaggaggta ttaaaaaaat
     2701 accattcttc cctaataatt catacttagc gaaagcaggt aataatgccg catcaccaga
     2761 cataattaaa gaagcttata acttttttag aaagggaatt agacatatag gtttaaatga
     2821 aaagataaga atatcttctt ttggggagag ggaggacgtc gtaaatgaaa cggccaaggt
     2881 tttatgcatg gttgacactg gtgtttccgg tatatctgta cgggaggttg aggcggaaat
     2941 accaataaaa atggatgagg aaatccctca agaaataaag gagataattg aagaagatta
     3001 taaatatcgt tatatgttct ctcatcctac tgatgaaggg gggagtgttt ctttccctat
     3061 ggggagagag tctgaaggca cccaaaaatt atttgaaatc atacctctaa ttagatctgc
     3121 gtttaatgat agtatggttg taattgttga tgagttagat aatagcttgc acccgcatat
     3181 tgctgacttg attgttaagt tatttaatga tcctgatgtc aataaaaaag gctcacaact
     3241 tgtattctca acccataata tgcagttaat ggctccagag aaaatgagaa gagatcagat
     3301 atggttttgt gaaaaaaata aaggtgcgtc gtctctttat agcttagatg attttgataa
     3361 gaaaaagata aaaacaacaa caccttacgc agcttggtat gatgagggta ggtttggtgg
     3421 ggttcccgac ataaactact taaaggtagc ctcatttata tctggtgata tatccttggt
     3481 aatgccggat attgacgtca aagaattgtc tgatggtttt tttgaagagt ttgacgggga
     3541 tctttctgat gagtaaaaaa catggaaata ataagaaagt cccacaagta atgtatatat
     3601 actgtgaggg tgagaaaacc gaaccattat accttgaggc ttatatttca gacagatcta
     3661 aaagaacttt aagtgtattt aaaataccta aaacaaggaa aaatacacct gagcagttgg
     3721 tggatgaggc gataaagaaa aagaattcat catcttccgc agaaggtgac gtcttttggg
     3781 ttgtttatga tcaagagcat ctgacgcctt cgtctatttc atgtcatcaa aaggcatgga
     3841 ataaagctaa taggcatggg ataaatatag caatatcctg tgtttgtttt gaattatggt
     3901 tattgttgca tttcggcttc acaaccaaag atttttcgtc gtatgataat ttgatgtcta
     3961 gctccccgct aaaaaaatta ataactaact atgataaagg tagtaaacta acttaccttg
     4021 ctataagaga gaggattggt actgcgaaag ataacgcaag aaagctagat atttacgcta
     4081 aaactaatta taaaaacacg cacgaacctt atctatttcc atgttataca tcattccatg
     4141 agctacttaa ggcgattgac gacttttgaa gtaatccatt gaccatggct tattacaggc
     4201 tgtaatctat gaaaaataaa aaccaccgct accaacggtg gtttttggaa ggtacatgag
     4261 ctaccaactt atgtaccggg taactggctt agagggagcg cagtcaccaa aaattgtcct
     4321 ttcagtttag ccttaactgg cgcagaactt caagactcac ttcctctaat ccgttaccaa
     4381 tggctgctgc cagtggcgat gaaccgtgtc tttccgggtt ggactcaaga tgatagttac
     4441 cggagaaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     4501 gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc
     4561 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccaccaggg
     4621 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag
     4681 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggcagcgg cgcatccttt
     4741 ttctgccccg tttgtcatgc catatgcctt cgctcttctc cgtgtgcagg taacgcccgc
     4801 cgcagccgaa cggcaggagc ggcagcgact gagtgagcga ggaggcgtca tatcctctga
     4861 acttccattt tctgccttac gcccgcagct gcattttcct ctgtcacatg aagcactttc
     4921 ctgattaccg cactcatgcc aacataataa gccagtatac actccgctag cgctacgtga
     4981 ctggttcagg gctgcgcccc gaaacccgct aaaagcgact gacgcgcctg cggcttgtcc
     5041 aacaccgtgc cgaccgggaa acatttcccg cccgtcccgg tgttatcagg aggctactct
     5101 ggcagacaaa cgcaacaaga tgctcacgat gtgggtgacg gaagacgaac accggcggct
     5161 gctcgagcgg tgcgacggca agcagctggc ggcgtggatg cgtcagacct gcctcgatga
     5221 gaagccggcg cgtgccggca gactgccgtc g
//
