LOCUS       NZ_CP102396             5596 bp    DNA     circular CON 27-AUG-2023
DEFINITION  Klebsiella pneumoniae strain K64 plasmid p6, complete sequence.
ACCESSION   NZ_CP102396
VERSION     NZ_CP102396.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30114398
            Assembly: GCF_024628885.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,G. and Luo,R.
  TITLE     Antimicrobial Susceptibility, Virulence, and Genomic Features of a
            Hypervirulent Serotype K64, ST11 Klebsiella pneumoniae isolated from
            blood of bone marrow transplant patient in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Ma,G. and Luo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2022) Zhejiang Key Laboratory of Digital
            Technology in Medical Diagnostics, Zhejiang Key Laboratory of
            Digital Technology in Medical Diagnostics, 329 Jinpeng Street, Xihu
            District, Hangzhou, Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-JUN-2022
            Assembly Method                   :: RAST v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 173.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/27/2023 04:43:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,925
            CDSs (total)                      :: 5,803
            Genes (coding)                    :: 5,619
            CDSs (with protein)               :: 5,619
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 73 of 184
            Pseudo Genes (incomplete)         :: 120 of 184
            Pseudo Genes (internal stop)      :: 26 of 184
            Pseudo Genes (multiple problems)  :: 35 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102396.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K64"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="p6"
                     /geo_loc_name="China: Beijing"
                     /collection_date="2021-12-19"
     gene            join(5183..5596,1..96)
                     /locus_tag="NRF21_RS29505"
                     /old_locus_tag="NRF21_29505"
     CDS             join(5183..5596,1..96)
                     /locus_tag="NRF21_RS29505"
                     /old_locus_tag="NRF21_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            103..315
                     /locus_tag="NRF21_RS29510"
                     /old_locus_tag="NRF21_29510"
     CDS             103..315
                     /locus_tag="NRF21_RS29510"
                     /old_locus_tag="NRF21_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            982..1206
                     /locus_tag="NRF21_RS29515"
                     /old_locus_tag="NRF21_29515"
     CDS             982..1206
                     /locus_tag="NRF21_RS29515"
                     /old_locus_tag="NRF21_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1190..1456
                     /locus_tag="NRF21_RS29520"
                     /old_locus_tag="NRF21_29520"
     CDS             1190..1456
                     /locus_tag="NRF21_RS29520"
                     /old_locus_tag="NRF21_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1526..1876)
                     /locus_tag="NRF21_RS29525"
                     /old_locus_tag="NRF21_29525"
     CDS             complement(1526..1876)
                     /locus_tag="NRF21_RS29525"
                     /old_locus_tag="NRF21_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1942..2487)
                     /locus_tag="NRF21_RS29530"
                     /old_locus_tag="NRF21_29530"
     CDS             complement(1942..2487)
                     /locus_tag="NRF21_RS29530"
                     /old_locus_tag="NRF21_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2513..2839)
                     /locus_tag="NRF21_RS29535"
                     /old_locus_tag="NRF21_29535"
     CDS             complement(2513..2839)
                     /locus_tag="NRF21_RS29535"
                     /old_locus_tag="NRF21_29535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3197..3355)
                     /locus_tag="NRF21_RS29660"
     CDS             complement(3197..3355)
                     /locus_tag="NRF21_RS29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3995..4171
                     /locus_tag="NRF21_RS29540"
                     /old_locus_tag="NRF21_29540"
     CDS             3995..4171
                     /locus_tag="NRF21_RS29540"
                     /old_locus_tag="NRF21_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4161..4505
                     /locus_tag="NRF21_RS29545"
                     /old_locus_tag="NRF21_29545"
     CDS             4161..4505
                     /locus_tag="NRF21_RS29545"
                     /old_locus_tag="NRF21_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4714..>5148
                     /locus_tag="NRF21_RS29550"
                     /old_locus_tag="NRF21_29550"
                     /pseudo=""
     CDS             4714..>5148
                     /locus_tag="NRF21_RS29550"
                     /old_locus_tag="NRF21_29550"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3830..3909
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3247..3644)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4..431
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.1900000000000003e-64"
     Typing          4496..5595
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.728"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgctgccga aggggacggc cgtagacagg acgcagagct ggacggtcgg gaacgggcgg
       61 tcgaagcaga acgcgctgag actggtgaag gagtaagtgc cgatgaccga gctggaaaaa
      121 cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct ggacgagtgg
      181 gagagcgcct tcggggaatg gcggaaaatg tcagggctta tgcaacggga gaacgcggcg
      241 ctgagcgagc gcgtgacgcg cttgagtcag caggtggaac gcttgagcgg gcaactgcag
      301 cgtttgagcc ggtagtgcag cgtcatgaga tggccgtggc cgcagaacgg gcgcacgagc
      361 aacggcagca cgaaaaggaa ctgacggaag cgcgttcccg gcagcgaagc tatgacggtc
      421 cgtcgctggg ctgaaatgca ggaaaacagg gttctcatcg ggcatttacg ctgaaaccgg
      481 atgtcattca cctggctgca tggctatgca gccaggtaaa atttatctgt cgcgaacggg
      541 gcggtttatc gggtggtttg ttgccggttt tacctgactg ccaccccgtt cgcggcgaac
      601 ccgtccgggg cggtgcgggc aacgggtgtt atgtttaatc agcgtgaacg gcagatacaa
      661 aaaagccccg gagggctttt aactactgac ttattttttt gaagatttgc tgggtttcaa
      721 tttgtcagta tttcccggcg tttttttatt gtcacgctgg cgtttatcag gttcccctgt
      781 tgaatcggca attcgtttcg gacccggctt aagacccata atcgttatcc tttcaatagt
      841 taagaggaaa gccctcaagc tagatatatt gaccgcgtta cggattatca agaggggcta
      901 acaagattat tttttgttct tttaggttga ttgtggtact gttgatatac atgtatagca
      961 aatggtgttc gggaggttta tatgcttgct atccggttat ctgatgagat tgagtcccgt
     1021 ctggactcgc tggcgaagca aaccggcaga acaaagacgt tttatgcgcg ggaagcaata
     1081 ctggcgcatc tggaagacct ggaggattat tatctttcag cagaaactgc tgcacgcgtt
     1141 cgccgtggtg atgaagcagt gcattcgtct gaagacgtga ggaagtcact tggtctggac
     1201 gattaactat tccgatcggg cgctcaaatc gttacgcaag atggacaaac agaacgcacg
     1261 acggattgtg gattttatga gtttacgcat tgcagttgct gccgatcctc gccagtcagg
     1321 gaagccgctc aaaggtgagc tgggcgagtt ctggcgctat cgggtgggag attatcgcgt
     1381 tctgtgtgag atccgagatg acgagcttgt tatccttgcc gccacgattg gacatcgccg
     1441 cgaagtttac gactgaagcc cgctatgcgg gcttttttga tgccgacaac aggtattatg
     1501 gtaaatactc gactaactac gtgttctact gtttgtcggt tttagttaac cagcaggttg
     1561 tcttaacgat aatgtagccg ttatttttaa ctaataaggt catattgtta acgtcaggca
     1621 taataaaaga cattatggcc tcagagttat catcagaagc agctctctgc attactactt
     1681 cgattccctt ttctttgaat ctataagtat ccagttgcct attaaactta gaaagaccta
     1741 caaccatact tagtttgtta tctttaacag taagagatgc ttcctgagta ccattacatc
     1801 ggtagtaagc ggtgtaaccg tcagcaaaag atcgaccggg gaatataaat gctaggaacg
     1861 caattaacca atacatagtt gagaatcttg gtattacaca tttcactatc ctctctttca
     1921 tttttcgttc accatacttt gttatctgat ttttaatttt tctattttat aaaccaagtc
     1981 tctaccaaaa aggcgaaaat tatcctcaaa taataataat tgtttaagaa catccatgaa
     2041 agcagctgct tcaccctcag ttatgggagc tactctatat aacatataac ctgagttaag
     2101 ggtttctttt atactttctt caatgctcat tataaatatt agtggattat catttttctt
     2161 cccccatggg tttctaatta atccttcgat gtactcaggt cctttgcgtc ctattgcctg
     2221 cgcaatattt tttatttcat ctgcgtgagg gctttgttgt acagctttgt ttatgcctgc
     2281 ttgagtgatt ttcagagact ctacaccgtg tatgttgtat gagataaaat gtaataaatt
     2341 aagatgcaat gcaattaatc catttttgtt tacatctgag tatttggata aaaaacgtaa
     2401 tgaagtgtta actctccctt gaagcctaac aattgggagt gctgccttgt atatttcctt
     2461 ctccgttaaa taccgtttaa aaaacataat tctccgcctc cgtatagcgt gcctatttta
     2521 tttcgtcgta aaacttagct ctgaaatatt cctttagaac catcatgtca ttaataattg
     2581 aatctatctc atccattttt tcttggtatt cttttaatgg tgaagggagc ggtaactcaa
     2641 tatttgaaaa gtgattggcg agatagggat caaggtttct catccattca ggcttttcgc
     2701 caatgcgttc ttcaactgaa agtgaacctg ataatctctg gttaataaga tcacttagac
     2761 tcattttggt tttattcgaa agccgttgta aggattgctt taatcctata tctagcatga
     2821 tatttgttct ttctctcatt accccccctt gctattgcgt tttttgttgc gcacaaagct
     2881 accattgcgc attaagaaac gcaataaaaa aggagggagg gagtggtttg tacatgatgt
     2941 gcagtgcgct gagggcatca gtggcgggtg tttttccgtg ggatggatgg agctccgggg
     3001 ataacagaac tgacgatcat ttaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3061 ctcttgcact gtaaacgaaa aaaccgccta gggaggcggt ttgatcgaag gttaagtcag
     3121 ttggggaact gcttaaccgg gtaactgggt atacgagacc gcagatacca aaactgttct
     3181 ttcagtgtag ccgcagttag gcacccactt caagacctct caatatctct ctcgtatata
     3241 tccagttacc aatggctgct gccagtggcg tttttgcgtg tccgtccggg ttggactcaa
     3301 gatgatagtt accggaaagg gcgcagcagt cgggctgaac ggggggttcg tgcatacagc
     3361 ccagcttgga gcgaacgacc taaaccgagc cgagatacca gcagcgtgag ctatgagaaa
     3421 gcgccacgct tcccgaaagg gaaaaaggcg gaacaggtat ccggtaaacg gcagggtcgg
     3481 aacaggagag cgcacgaggg agccgccgga gagaaatcac cggcatcttt aagtcctgtc
     3541 gggtttcgtc ctcctctgac ttgagcgtcc atttctgtga tgctcgtcag gggaggcgga
     3601 gcctatggaa aaacggcaac gccgcatcct tttctccggc cctttctgat ttgagcgcct
     3661 ttgctttatc ccgttatccg tgagtcggtc acgcccgccg cagccgaacg gcaggagcgc
     3721 agctaccgag tgagcgagga ggcgtcattt catctgtccg tgcatggtat tgcggacgct
     3781 cctgagccac atatcacacc tgccacatga ggcactttca tgaatcgtgc tctcatgcca
     3841 acatagtaag ccagtataca cagcgtaaac gctgtgactg gttcagggct gcgccccgaa
     3901 acccgctaac accactgacg cgctggcgct tgtccaacgc cggagcgacc cggaaaactt
     3961 ttccgtgccg tccggcgttt gcgaaggagg gataatgcag aagccgaaga agaagccgct
     4021 cgtcgtaaaa aaaagatcct ggcatccggc agtgatggca aatctgatgc gtcaggcaca
     4081 ggaggaagat taccgccggg agcagatggc agagcttgag cgttcagccc ggtcacattt
     4141 tgcggatatc ggaggagagg atggcggtta agcgtacccg ttttttaggt atccgggtga
     4201 ccgatgggga gtaccagcag ctgctggagc gctgcaatgg aaggcaactg gcggtgtgga
     4261 tgcgggaaac ctgtctcgat acgcgcccgg cgcggtcgtt gcggctgccg tccatcgatc
     4321 cggttttgct gcgccagctt gccgggatgg ggaacaacct caaccagatc gcccggaaaa
     4381 ttaacggcgg ccagtggtca ggcgctgacc gggttcaggt ggtggccgcg ctgatggcca
     4441 ttgatgccga actcgagcgg ctgcggcatg ccgtgctgga aaagggggca gacgatgatc
     4501 gttaagtttc atccgcgagg gcgaggcggc ggtgccgggc cggtggatta tctgctgggg
     4561 aaagaccgcc agcgcgacgg ggccatcgtc ctgcagggaa agccggagga ggtccgggag
     4621 cttatcgacg cctcgcccta cgccaaaaag tacacctccg gcgtcctgtc ttttgctgaa
     4681 caggatttac cgcccggcca gcgtgaaaag ctgatggcga gcttcgagcg ggttctgatg
     4741 cccggactcg ataaagacca gtacagcgtg ctgtgggttg aacaccggga caaggggcgg
     4801 ctggaactga attttctggt accgaacacg gaactgctga ccggcaggcg tctccagccg
     4861 tactacgacc gggcagaccg tccgcgcatc gatgcgtggc agaccgtggt gaacgggcga
     4921 ctggggctgc acgacccgaa cgcaccggag aaccggcgcg cgctggtgac gccatcttcg
     4981 ttacccaaaa cgaagcagga agccgcagag gctattacgc ggggcttact ggccctcgcc
     5041 tcgtcagggg agcttaaaac gcgtcaggac gtcactgagg cgctggaaag cgcaggtttt
     5101 gaggtcgtgc gcaccacaaa gagcagcatc agcattgccg acccggacgg agggcgaaac
     5161 atccgactta agggagccat ctatgaacag tcttttaacg ctggcgaagg acttagagca
     5221 gaaatcgaaa gcgcagcagc agagtaccgg cgaaatgctg aaagccgcat tcagcgagca
     5281 cgagaagtct gtcagagcgg aactgagcga aagcgggaag agaatcagcg ccgccatccg
     5341 cgcccacgag cagggtatga gcgcggccat gcagtcgaac cgcctgagcg tgatgcgtat
     5401 ggtcgggcgg acatggctga ccatcacgat ggtgtcagga ctgctgttcg ccagcctgag
     5461 cggggtgttg tggtatcagg ggagcttgat agcgtcaaat ctggccgaaa tcgaccggca
     5521 gaacgcagcg ctgtcgaagc tgaacgcgaa gacctggggc gtgacgtacc tggaagacag
     5581 caacggacgt tttctg
//
