LOCUS       NZ_CP102191             5596 bp    DNA     linear   CON 23-AUG-2023
DEFINITION  Klebsiella pneumoniae strain S234 plasmid pS234-5, complete
            sequence.
ACCESSION   NZ_CP102191
VERSION     NZ_CP102191.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30074620
            Assembly: GCF_024584455.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Plasmid recombination events occurring during conjugation promote
            the spread of ST11 hypervirulent carbapenem-resistant Klebsiella
            pneumoniae in central China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Department of Clinical Laboratory, The First
            Affiliated Hospital of Nanchang University, Yong Wai Zheng Jie No.
            17, Nanchang 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 32.0x
            Sequencing Technology             :: PacBio Sequel2
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/23/2023 05:10:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,920
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 69 of 183
            Pseudo Genes (incomplete)         :: 124 of 183
            Pseudo Genes (internal stop)      :: 23 of 183
            Pseudo Genes (multiple problems)  :: 33 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102191.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="S234"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pS234-5"
                     /geo_loc_name="China: Jiangxi"
                     /collection_date="2017-11-16"
     gene            complement(<1..381)
                     /locus_tag="NQZ63_RS29475"
                     /old_locus_tag="NQZ63_29475"
     CDS             complement(<1..381)
                     /locus_tag="NQZ63_RS29475"
                     /old_locus_tag="NQZ63_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159225048.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIK"
                     /name="hypothetical protein"
     gene            complement(407..733)
                     /locus_tag="NQZ63_RS29480"
                     /old_locus_tag="NQZ63_29480"
     CDS             complement(407..733)
                     /locus_tag="NQZ63_RS29480"
                     /old_locus_tag="NQZ63_29480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1091..1249)
                     /locus_tag="NQZ63_RS29630"
     CDS             complement(1091..1249)
                     /locus_tag="NQZ63_RS29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1889..2065
                     /locus_tag="NQZ63_RS29485"
                     /old_locus_tag="NQZ63_29485"
     CDS             1889..2065
                     /locus_tag="NQZ63_RS29485"
                     /old_locus_tag="NQZ63_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2055..2399
                     /locus_tag="NQZ63_RS29490"
                     /old_locus_tag="NQZ63_29490"
     CDS             2055..2399
                     /locus_tag="NQZ63_RS29490"
                     /old_locus_tag="NQZ63_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2608..>3042
                     /locus_tag="NQZ63_RS29495"
                     /old_locus_tag="NQZ63_29495"
                     /pseudo=""
     CDS             2608..>3042
                     /locus_tag="NQZ63_RS29495"
                     /old_locus_tag="NQZ63_29495"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3077..3586
                     /locus_tag="NQZ63_RS29500"
                     /old_locus_tag="NQZ63_29500"
     CDS             3077..3586
                     /locus_tag="NQZ63_RS29500"
                     /old_locus_tag="NQZ63_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3593..3805
                     /locus_tag="NQZ63_RS29505"
                     /old_locus_tag="NQZ63_29505"
     CDS             3593..3805
                     /locus_tag="NQZ63_RS29505"
                     /old_locus_tag="NQZ63_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4472..4696
                     /locus_tag="NQZ63_RS29510"
                     /old_locus_tag="NQZ63_29510"
     CDS             4472..4696
                     /locus_tag="NQZ63_RS29510"
                     /old_locus_tag="NQZ63_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4680..4946
                     /locus_tag="NQZ63_RS29515"
                     /old_locus_tag="NQZ63_29515"
     CDS             4680..4946
                     /locus_tag="NQZ63_RS29515"
                     /old_locus_tag="NQZ63_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5016..5366)
                     /locus_tag="NQZ63_RS29520"
                     /old_locus_tag="NQZ63_29520"
     CDS             complement(5016..5366)
                     /locus_tag="NQZ63_RS29520"
                     /old_locus_tag="NQZ63_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1724..1803
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1141..1538)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2390..3921
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttatactt tcttcaatgc tcattataaa tattagtgga ttatcatttt tcttccccca
       61 tgggtttcta attaatcctt cgatgtactc aggtcctttg cgtcctattg cctgcgcaat
      121 attttttatt tcatctgcgt gagggctttg ttgtacagct ttgtttatgc ctgcttgagt
      181 gattttcaga gactctacac cgtgtatgtt gtatgagata aaatgtaata aattaagatg
      241 caatgcaatt aatccatttt tgtttacatc tgagtatttg gataaaaaac gtaatgaagt
      301 gttaactctc ccttgaagcc taacaattgg gagtgctgcc ttgtatattt ccttctccgt
      361 taaataccgt ttaaaaaaca taattctccg cctccgtata gcgtgcctat tttatttcgt
      421 cgtaaaactt agctctgaaa tattccttta gaaccatcat gtcattaata attgaatcta
      481 tctcatccat tttttcttgg tattctttta atggtgaagg gagcggtaac tcaatatttg
      541 aaaagtgatt ggcgagatag ggatcaaggt ttctcatcca ttcaggcttt tcgccaatgc
      601 gttcttcaac tgaaagtgaa cctgataatc tctggttaat aagatcactt agactcattt
      661 tggttttatt cgaaagccgt tgtaaggatt gctttaatcc tatatctagc atgatatttg
      721 ttctttctct cattaccccc ccttgctatt gcgttttttg ttgcgcacaa agctaccatt
      781 gcgcattaag aaacgcaata aaaaaggagg gagggagtgg tttgtacatg atgtgcagtg
      841 cgctgagggc atcagtggcg ggtgtttttc cgtgggatgg atggagctcc ggggataaca
      901 gaactgacga tcatttaaag gatcttcttg agatcctttt tttctgcgcg taatctcttg
      961 cactgtaaac gaaaaaaccg cctggggagg cggtttgatc gaaggttaag tcagttgggg
     1021 aactgcttaa ccgggtaact gggtatacga gaccgcagat accaaaactg ttctttcagt
     1081 gtagccgcag ttaggcaccc acttcaagac ctctcaatat ctctctcgta tatatccagt
     1141 taccaatggc tgctgccagt ggcgtttttg cgtgtccgtc cgggttggac tcaagatgat
     1201 agttaccgga aagggcgcag cagtcgggct gaacgggggg ttcgtgcata cagcccagct
     1261 tggagcgaac gacctaaacc gagccgagat accagcagcg tgagctatga gaaagcgcca
     1321 cgcttcccga aagggaaaaa ggcggaacag gtatccggta aacggcaggg tcggaacagg
     1381 agagcgcacg agggagccgc cggagagaaa tcaccggcat ctttaagtcc tgtcgggttt
     1441 cgtcctcctc tgacttgagc gtccatttct gtgatgctcg tcaggggagg cggagcctat
     1501 ggaaaaacgg caacgccgca tccttttctc cggccctttc tgatttgagc gcctttgctt
     1561 tatcccgtta tccgtgagtc ggtcacgccc gccgcagccg aacggcagga gcgcagctac
     1621 cgagtgagcg aggaggcgtc atttcatctg tccgtgcatg gtattgcgga cgctcctgag
     1681 ccacatatca cacctgccac atgaggcact ttcatgaatc gtgctctcat gccaacatag
     1741 taagccagta tacacagcgt aaacgctgtg actggttcag ggctgcgccc cgaaacccgc
     1801 taacaccact gacgcgctgg cgcttgtcca acgccggagc gacccggaaa acttttccgt
     1861 gccgtccggc gtttgcgaag gagggataat gcagaagccg aagaagaagc cgctcgtcgt
     1921 aaaaaaaaga tcctggcatc cggcagtgat ggcaaatctg atgcgtcagg cacaggagga
     1981 agattaccgc cgggagcaga tggcagagct tgagcgttca gcccggtcac attttgcgga
     2041 tatcggagga gaggatggcg gttaagcgta cccgtttttt aggtatccgg gtgaccgatg
     2101 gggagtacca gcagctgctg gagcgctgca atggaaggca actggcggtg tggatgcggg
     2161 aaacctgtct cgatacgcgc ccggcgcggt cgttgcggct gccgtccatc gatccggttt
     2221 tgctgcgcca gcttgccggg atggggaaca acctcaacca gatcgcccgg aaaattaacg
     2281 gcggccagtg gtcaggcgct gaccgggttc aggtggtggc cgcgctgatg gccattgatg
     2341 ccgaactcga gcggctgcgg catgccgtgc tggaaaaggg ggcagacgat gatcgttaag
     2401 tttcatccgc gagggcgagg cggcggtgcc gggccggtgg attatctgct ggggaaagac
     2461 cgccagcgcg acggggccat cgtcctgcag ggaaagccgg aggaggtccg ggagcttatc
     2521 gacgcctcgc cctacgccaa aaagtacacc tccggcgtcc tgtcttttgc tgaacaggat
     2581 ttaccgcccg gccagcgtga aaagctgatg gcgagcttcg agcgggttct gatgcccgga
     2641 ctcgataaag accagtacag cgtgctgtgg gttgaacacc gggacaaggg gcggctggaa
     2701 ctgaattttc tggtaccgaa cacggaactg ctgaccggca ggcgtctcca gccgtactac
     2761 gaccgggcag accgtccgcg catcgatgcg tggcagaccg tggtgaacgg gcgactgggg
     2821 ctgcacgacc cgaacgcacc ggagaaccgg cgcgcgctgg tgacgccatc ttcgttaccc
     2881 aaaacgaagc aggaagccgc agaggctatt acgcggggct tactggccct cgcctcgtca
     2941 ggggagctta aaacgcgtca ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtc
     3001 gtgcgcacca caaagagcag catcagcatt gccgacccgg acggagggcg aaacatccga
     3061 cttaagggag ccatctatga acagtctttt aacgctggcg aaggacttag agcagaaatc
     3121 gaaagcgcag cagcagagta ccggcgaaat gctgaaagcc gcattcagcg agcacgagaa
     3181 gtctgtcaga gcggaactga gcgaaagcgg gaagagaatc agcgccgcca tccgcgccca
     3241 cgagcagggt atgagcgcgg ccatgcagtc gaaccgcctg agcgtgatgc gtatggtcgg
     3301 gcggacatgg ctgaccatca cgatggtgtc aggactgctg ttcgccagcc tgagcggggt
     3361 gttgtggtat caggggagct tgatagcgtc aaatctggcc gaaatcgacc ggcagaacgc
     3421 agcgctgtcg aagctgaacg cgaagacctg gggcgtgacg tacctggaag acagcaacgg
     3481 acgttttctg gtgctgccga aggggacggc cgtagacagg acgcagagct ggacggtcgg
     3541 gaacgggcgg tcgaagcaga acgcgctgag actggtgaag gagtaagtgc cgatgaccga
     3601 gctggaaaaa cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct
     3661 ggacgagtgg gagagcgcct tcggggaatg gcggaaaatg tcagggctta tgcaacggga
     3721 gaacgcggcg ctgagcgagc gcgtgacgcg cttgagtcag caggtggaac gcttgagcgg
     3781 gcaactgcag cgtttgagcc ggtagtgcag cgtcatgaga tggccgtggc cgcagaacgg
     3841 gcgcacgagc aacggcagca cgaaaaggaa ctgacggaag cgcgttcccg gcagcgaagc
     3901 tatgacggtc cgtcgctggg ctgaaatgca ggaaaacagg gttctcatcg ggcatttacg
     3961 ctgaaaccgg atgtcattca cctggctgca tggctatgca gccaggtaaa atttatctgt
     4021 cgcgaacggg gcggtttatc gggtggtttg ttgccggttt tacctgactg ccaccccgtt
     4081 cgcggcgaac ccgtccgggg cggtgcgggc aacgggtgtt atgtttaatc agcgtgaacg
     4141 gcagatacaa aaaagccccg gagggctttt aactactgac ttattttttt gaagatttgc
     4201 tgggtttcaa tttgtcagta tttcccggcg tttttttatt gtcacgctgg cgtttatcag
     4261 gttcccctgt tgaatcggca attcgtttcg gacccggctt aagacccata atcgttatcc
     4321 tttcaatagt taagaggaaa gccctcaagc tagatatatt gaccgcgtta cggattatca
     4381 agaggggcta acaagattat tttttgttct tttaggttga ttgtggtact gttgatatac
     4441 atgtatagca aatggtgttc gggaggttta tatgcttgct atccggttat ctgatgagat
     4501 tgagtcccgt ctggactcgc tggcgaagca aaccggcaga acaaagacgt tttatgcgcg
     4561 ggaagcaata ctggcgcatc tggaagacct ggaggattat tatctttcag cagaaactgc
     4621 tgcacgcgtt cgccgtggtg atgaagcagt gcattcgtct gaagacgtga ggaagtcact
     4681 tggtctggac gattaactat tccgatcggg cgctcaaatc gttacgcaag atggacaaac
     4741 agaacgcacg acggattgtg gattttatga gtttacgcat tgcagttgct gccgatcctc
     4801 gccagtcagg gaagccgctc aaaggtgagc tgggcgagtt ctggcgctat cgggtgggag
     4861 attatcgcgt tctgtgtgag atccgagatg acgagcttgt tatccttgcc gccacgattg
     4921 gacatcgccg cgaagtttac gactgaagcc cgctatgcgg gcttttttga tgccgacaac
     4981 aggtattatg gtaaatactc gactaactac gtgttctact gtttgtcggt tttagttaac
     5041 cagcaggttg tcttaacgat aatgtagccg ttatttttaa ctaataaggt catattgtta
     5101 acgtcaggca taataaaaga cattatggcc tcagagttat catcagaagc agctctctgc
     5161 attactactt cgattccctt ttctttgaat ctataagtat ccagttgcct attaaactta
     5221 gaaagaccta caaccatact tagtttgtta tctttaacag taagagatgc ttcctgagta
     5281 ccattacatc ggtagtaagc ggtgtaaccg tcagcaaaag atcgaccggg gaatataaat
     5341 gctaggaacg caattaacca atacatagtt gagaatcttg gtattacaca tttcactatc
     5401 ctctctttca tttttcgttc accatacttt gttatctgat ttttaatttt tctattttat
     5461 aaaccaagtc tctaccaaaa aggcgaaaat tatcctcaaa taataataat tgtttaagaa
     5521 catccatgaa agcagctgct tcaccctcag ttatgggagc tactctatat aacatataac
     5581 ctgagttaag ggtttc
//
