LOCUS       NZ_CP102189            11970 bp    DNA     linear   CON 23-AUG-2023
DEFINITION  Klebsiella pneumoniae strain S234 plasmid pS234-3, complete
            sequence.
ACCESSION   NZ_CP102189
VERSION     NZ_CP102189.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30074620
            Assembly: GCF_024584455.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Plasmid recombination events occurring during conjugation promote
            the spread of ST11 hypervirulent carbapenem-resistant Klebsiella
            pneumoniae in central China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Department of Clinical Laboratory, The First
            Affiliated Hospital of Nanchang University, Yong Wai Zheng Jie No.
            17, Nanchang 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 32.0x
            Sequencing Technology             :: PacBio Sequel2
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/23/2023 05:10:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,920
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 69 of 183
            Pseudo Genes (incomplete)         :: 124 of 183
            Pseudo Genes (internal stop)      :: 23 of 183
            Pseudo Genes (multiple problems)  :: 33 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102189.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="S234"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pS234-3"
                     /geo_loc_name="China: Jiangxi"
                     /collection_date="2017-11-16"
     gene            complement(<1..264)
                     /locus_tag="NQZ63_RS28815"
                     /old_locus_tag="NQZ63_28815"
                     /pseudo=""
     CDS             complement(<1..264)
                     /locus_tag="NQZ63_RS28815"
                     /old_locus_tag="NQZ63_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011178375.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /name="cloacin"
     gene            complement(369..1856)
                     /gene="ltrA"
                     /locus_tag="NQZ63_RS28820"
                     /old_locus_tag="NQZ63_28820"
     CDS             complement(369..1856)
                     /gene="ltrA"
                     /locus_tag="NQZ63_RS28820"
                     /old_locus_tag="NQZ63_28820"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            2501..2746
                     /locus_tag="NQZ63_RS28825"
                     /old_locus_tag="NQZ63_28825"
     CDS             2501..2746
                     /locus_tag="NQZ63_RS28825"
                     /old_locus_tag="NQZ63_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3020..3391
                     /gene="tolA"
                     /locus_tag="NQZ63_RS28830"
                     /old_locus_tag="NQZ63_28830"
     CDS             3020..3391
                     /gene="tolA"
                     /locus_tag="NQZ63_RS28830"
                     /old_locus_tag="NQZ63_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3388..3753
                     /locus_tag="NQZ63_RS28835"
                     /old_locus_tag="NQZ63_28835"
     CDS             3388..3753
                     /locus_tag="NQZ63_RS28835"
                     /old_locus_tag="NQZ63_28835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3928..4206
                     /locus_tag="NQZ63_RS28840"
                     /old_locus_tag="NQZ63_28840"
     CDS             3928..4206
                     /locus_tag="NQZ63_RS28840"
                     /old_locus_tag="NQZ63_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4194..4508
                     /locus_tag="NQZ63_RS28845"
                     /old_locus_tag="NQZ63_28845"
     CDS             4194..4508
                     /locus_tag="NQZ63_RS28845"
                     /old_locus_tag="NQZ63_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4672..5100
                     /locus_tag="NQZ63_RS28850"
                     /old_locus_tag="NQZ63_28850"
     CDS             4672..5100
                     /locus_tag="NQZ63_RS28850"
                     /old_locus_tag="NQZ63_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5126..5305
                     /locus_tag="NQZ63_RS28855"
                     /old_locus_tag="NQZ63_28855"
     CDS             5126..5305
                     /locus_tag="NQZ63_RS28855"
                     /old_locus_tag="NQZ63_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5332..5862)
                     /locus_tag="NQZ63_RS28860"
                     /old_locus_tag="NQZ63_28860"
     CDS             complement(5332..5862)
                     /locus_tag="NQZ63_RS28860"
                     /old_locus_tag="NQZ63_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5869..6600)
                     /gene="mobC"
                     /locus_tag="NQZ63_RS28865"
                     /old_locus_tag="NQZ63_28865"
     CDS             complement(5869..6600)
                     /gene="mobC"
                     /locus_tag="NQZ63_RS28865"
                     /old_locus_tag="NQZ63_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6600..8564)
                     /locus_tag="NQZ63_RS28870"
                     /old_locus_tag="NQZ63_28870"
     CDS             complement(6600..8564)
                     /locus_tag="NQZ63_RS28870"
                     /old_locus_tag="NQZ63_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(10048..10197)
                     /locus_tag="NQZ63_RS28875"
                     /old_locus_tag="NQZ63_28875"
     CDS             complement(10048..10197)
                     /locus_tag="NQZ63_RS28875"
                     /old_locus_tag="NQZ63_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10282..10539)
                     /locus_tag="NQZ63_RS28880"
                     /old_locus_tag="NQZ63_28880"
     CDS             complement(10282..10539)
                     /locus_tag="NQZ63_RS28880"
                     /old_locus_tag="NQZ63_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(10549..>11970)
                     /locus_tag="NQZ63_RS28885"
                     /old_locus_tag="NQZ63_28885"
     CDS             complement(10549..>11970)
                     /locus_tag="NQZ63_RS28885"
                     /old_locus_tag="NQZ63_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_257105235.1"
                     /translation="GGQSSATAMAFGLPALATPGAEGLALSVSGDALSAAVADVLAALK
                     GPFKFGLWGIAIYGVLPSEIAKDDPKMMSKIMTSLPADAVTETPASTLPLDQATVRVRQ
                     RVVDVVKDERQHIAVVAGRPMSVPVVDAKPTKRPGVFSVSIPGLPSLQVSVPKGVPTAK
                     APPKGIVAEKGDSRPAGFTAGGNSREAVIRFPKETGQKPVYVSVTDVLTPAQVKQRQEE
                     EKRRQQAWDAAHPEEGLKRDYDKAKAELDAEDKNIATLNSRIASTEKALPGARAAVQEA
                     DKKVKEAEANKDDFVTYNPPHEYGSGWQDQVRYLDKDIQNQNEKLKAAQASLNAMNESL
                     SRDKAALTGAMESRKQKEKKAKDAENKLNEEKNKPRKGTKDYGHDYHPVPKTEDIKGLG
                     ELKRGDPKTPKQGGGGKRARWYGDKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGK
                     QVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             5549..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9367..9945
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5873..6600)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 accagagcta ccgctatgat tgccgctgtt accaccatta ccaccgggtt tactcccgcc
       61 gttaccaaac tcccccggat tatagttatg aatatctcca ttcggcgtat gcgtcgtacc
      121 ccagcccggc ccgctgtttg gatccgtacc accacctgat gaagggccac ctttaccaga
      181 cgtaccgttc acattcccac tggcctgacc accattatgt cccagacctg aattacccgg
      241 acctcgaccg tctccaccac tcataccgtt ttcctctttg acatttaaaa caataaatta
      301 aagccaacat gtcgcggtag ggatacccgt taccggatac cccccgcaca gatcccggcg
      361 tgcgcgattt acgcaccggg ctcctgcctc gggtgtctgg cggtgaaccg ctccacaggc
      421 catggatgaa gaacccgaac ccttggtagc catgcggctg ccagtttgtt tgctttcgtc
      481 caggtcgtat catccttctg gctcctgcgc ctgagcgccc ggcgccagag gtttgttacg
      541 tgtgtcctga acttctgcat ggtggggaag ttgcccggta ccgagtgata gttcaggtat
      601 ccctgaacca ctctcctgag ccattttccc tgttcgggga ttgagtaatg ccagcgcctt
      661 cgcagaccgt ctttgatggc tttcagagtt gccgtcatcc gatcccggcg ggtctttcgt
      721 atcagcatga acctgccgtt gcgatctttc ccgctgatgt gcgtgaaccc gaggaagttg
      781 aacgtttctg gtttgccttt tcccctgatg gcacggtttt cggcagcgaa gcggccgaac
      841 tccatcagac gggttttctc cgggtgaacc gtgagtccga actccctcag tctgcgctgc
      901 atggctatac ggaagcgccg ggcatcgtat cgtttgtcga acccgatgac gatgtcatcg
      961 gcgtatctga ccattaccac attgcctgtg gcatagcgac gtcgccactg atgcgcccac
     1021 agatcgaaga cgtagtggag gtatatgttt gccagcagcg gtgagatgac cgcaccctgt
     1081 ggggtgcctt cctccgttgc tcgccattga ccctcctccg acgtcccggc tgtgagccac
     1141 ttacgtatga gcctgattac cctccggtcg ccgatccgat gctctgtgaa cctgatcagc
     1201 cattcgtggc tcaccctgtc gaagaactga ctgatgtcgg catccagtac ccagtttacg
     1261 ttagtgcgta ccagccctgt ggccagtgcg tccagtgcat cgtgctggct tcgcccgggt
     1321 ctgaacccgt atgagaaccc cataaagtcg ttttcataga ctgcgttcag gattttcacc
     1381 agcgcatact ggacgatctt gtcctccagc gaggcgatgc cgagcgggcg ttgttttcca
     1441 tccgcttttg ggatgtagtg acgcctgccg ggctgcgccc tgtagctgcc ctgatgtagc
     1501 ctccggtgca gatctgttat gttgttcttc atgtttccgg cgtagtccat ccacctgatg
     1561 ccatccactc cggcggccgc tttcctgctc agggagagga atgcggcttc cagtgcttcg
     1621 actgtcagca ggtggaacaa tgctgtaaac cgttctttct tccgctgctt cgcagcttcc
     1681 cgcacgcgtg acagcctctg tgacatgctt tcccggctct gtgtccggcg catgtgtggc
     1741 tgttccgcgt tccccttggc cccgctcctt cgctccactg actccgctcc tttcgggttg
     1801 ttcgcctgct tcgccgctac tatgagcgag tccgacttct cctctccgta catcaccggc
     1861 tatgactcct cgtcttcccg gtgcgggcca tctccgacac tggcagatgg tcagagggga
     1921 gatctcccgg ttcccgcgta gagatcgtat tgacatgcca gggtctcaga ccccgccggg
     1981 tccatgtggc actcgcagtg tcgcacccta tgatgttgcc ttccgttaac agtacaacgt
     2041 cggcacccgg taatttaata tacatttcgt ggctcaatgg ctggcctgtc aacacccctg
     2101 tcaacgcttc gccccatacc tcgcggtatg caacgcatga ctcggggacc ttgtggattg
     2161 ctggtccttc aatggtcggg gactttcacc ccttgatctc taaccggtct cccggcgcac
     2221 actgtacata tacacagtat aattattacc ttactcctcc cgttttcagg tgtcaatata
     2281 tgagattagt tctcatcttc aggcacaaaa aagcgcccgg agccacctgt aagcagatcg
     2341 cgtcttttcc cgaaaatttc tcatggcaac aaattttatt atttaagaca ttcatcacac
     2401 ttacatttca gatggtatag acacatctga aagctaactg gcatgaggag aggacctgcg
     2461 gttaaactgt tcgggaaacc tgagacgccc ggaggccact atgacgcacc tgaaactgac
     2521 tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct caggcactgg ccatgggcct
     2581 gaaagggaac aaaacgctgc caaaggcggt aaaacaacag gtgcgggaaa gggtgcttcg
     2641 tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa atgcgtcggg agaaatacga
     2701 ggctacggaa accaccttca cctggcagcc acagcgccgc cggtaagggg tatttaccat
     2761 aacgtcgcat tgcccgcacc gctcagacgg tctgagggcg tttccggcgg ctacgggata
     2821 aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg cccggtaaat tgtaacccgc
     2881 tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt gtaaaaacga tacgataagc
     2941 cgtgtttatg taaatgaacg tggccgtaaa gagtgacaaa tatacatgta acatttttta
     3001 ctgacaggtc atcaacccga tgcgtttaat accctttatc ctgctggcat tgtgcagccc
     3061 cttactcctg acggcctgta agcattccac cacccagccg ccgaaaattt atactctgga
     3121 taagtcatct aaaatcagta gctacgctgc ggccatcaag accgctgttg aaatgcagat
     3181 gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg cggatatcca tacaacgtga
     3241 tggcactctg aacagcgcca ttgctgaagg aggagatccg aaactctgta aagcagcaat
     3301 atcagccgtt acccacgcca aaattccgcc ggcaccggat gaaaatacct ggcaaatatt
     3361 caaaaacgcc cctctggatt tcagaccatg aaagctttta ctctgacagt gctggcggcc
     3421 atgttcctca ctccggtaca tgcgatacca ctaaaacccg taaccacact gcagccaacc
     3481 tacccggtac aggccgcagc aaaacgcatc gaaggtcagg tgaccgccac gtttgatatt
     3541 ctggacgatg gacgagtgga caacgtgtcg cttaccggct gtcacctttt ttttcgggaa
     3601 acgctgacgg caatgaggca ctggctatat cccgcagggc acccggcaat gcgacaggtt
     3661 ataaattttg aattcagtct gaaaaatgac ggcttacagg atccgcgacc agtcaccacc
     3721 caaaagctca atgatgatgg ttgccgccag taacaggtcc tttaacccgc aggctgcaat
     3781 agtcctggat gatgagtgag cctgtagcag ccctgattag tggaagcgaa gctgaaaaat
     3841 gggcagcaaa agccgtattt ctggcaaata gcgccgccac ggattaaagt agtcatgtgt
     3901 aatacatatg actacaaagg ggcttttatg tcatcagcaa catccatcag gctcgatgaa
     3961 gaacttaagg accggctgaa aacgctggcc gatgaccggc accgctcagc gcacgcgcta
     4021 atgctggagg caatcaccga atacatcgac cgggaagaga agcgcagcca gtacctgcgg
     4081 gacggacagg ccgcatggca gcactatcag gaaacgggtc tgcaccttac tgcggaagaa
     4141 gcggaagcct ggataagtac ctggggaact gagaacgagc aggatgctcc gccatgccac
     4201 aggtaacgat ttccgcgctg gcacagcgcg atttacaacg ccttcaggac tttctcaaaa
     4261 ccaaaaatcg gctggcagcc agaaaaggag gtgaggtgat cgtccgggct atccagcaac
     4321 tgaaaacatt gccagacatt ggccgcccgg tgccgtttct gccgctggaa tataaggaac
     4381 tggtgatcgg gtttggcgac agtggctatg tgatgctcta ccgccacgac cgggaaatgg
     4441 accagattgt gatcgtcacc gtcaggcatc agaaagaatc cgggtatccg ggggcagaca
     4501 gcctctaata accgatgacg ggggcgcaat tcattgcgtg gccgtcgccg gtttgacaag
     4561 cgcagcgcgt cagaaaacca catcctgaac ggaaaaacgg gtaaaattca gcgatacatt
     4621 taccgtgaga aattacacac ctttcacacc cgataaaatc aggggtatac agtggcgaaa
     4681 acattgctca acctttcaca ggcagcacaa gccgcgggca ttaccagaag aacgctatac
     4741 aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg ggaaaaacaa ccctgtcgtg
     4801 gatgtgtcag aactgatccg tgtatacgga aatgtgaata tccctgagaa acaaattccc
     4861 ggcatttcac accgtgaaaa tacacaaaaa aatttcccac aggaacagct tctggcaatg
     4921 caaaaggaac tggccgatct ccggcaggct gtcaccctga tgctggaaga taaaacatcc
     4981 cgggaagaag aacgccgcca gcacgatgat gagcgccgga aactccaggc cgaagtggac
     5041 agattgacaa cagaactgac acagaagaaa aaacgcttct ggtccggctg gtttagctga
     5101 gaacttaccg aagggaatgg agaacatgac gaagcaggaa aaaactgccc tcaatatggc
     5161 taaattcatt aaggatcagt cgctgctgtt gctggaaaag ctgaacgagc tggacatgga
     5221 taccccggcc gacatgtgcg aacggcttca cgaggatgcg gaaacactct ggcaggtgat
     5281 gcgggataag ctcggggaag aataacagca ccggccatgc cggtgctggc gttatttttt
     5341 gccgaacggc caccaccggc gggcgggttt gtcctccggc agttcaggca ccggcacgtt
     5401 aaaatagcgg ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct
     5461 ttccccgtgc cgctcgctga ccacctcaat catcgcgtta atctgctccg gtgagcactt
     5521 cccggcatca accacctgca caccgttctc gatggcgaac cgctccacct ttcgatagag
     5581 gtaatcacac agcttcattc ctgtgggttt gtcccgttcc cattgcggat tttcaccggt
     5641 gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt
     5701 catcatcgcc agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca
     5761 ggcgagcacc accatgtctt ccagatgctc gctggttggc ataatgcagc gctcgccgtc
     5821 tttgtcttcc acgtcctcaa taaataccat gtacagcgcc ataatccctc actccgttgt
     5881 cataaattta aagcacgcga acagcctctc ccggtccagc tccaccgttt tgcgggcctc
     5941 gccggtctgt ggattcacga tttcatccac cgccagaatc gcccgctgta cccggacaag
     6001 cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc ggcggttgaa
     6061 cagatacccg gacaccactt cctgataccg cctgcggctc ttgacggtgc gctctacctc
     6121 aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc ggcactttca tccctttcag
     6181 ggccatacgg tgcaggtacc gccagccggt ggccccccgg ccctccatgg agaggcgcag
     6241 ggactgcacg gccagccggt gcggcagctg cgccgccgac acccttgacg gctgaaacgg
     6301 aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt gcggcgtcag
     6361 cccccacacg ctgacctgac ggccgtcaac cgggtatttc gcccggacca gcagcccttt
     6421 cgccaccaga ctgtccagga agcggtaaac cggctgtacc gccgcaatcc ccagccgtcg
     6481 cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg tcagccgtgt
     6541 tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct ccagtgtcat
     6601 cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc cggaggcagg
     6661 tactccgcca gcgggtccgt tttcgccagc accgggaagg ttttcagcga aatttcccgg
     6721 cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc
     6781 gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc
     6841 tggatgaagc tgtcgccgct gatgtgctcc acgttccccg cctcccggct ggcccggcgg
     6901 cgctccacgt ccacccggat ctccccggtc tggcccgccg cccactgcgc ggactcctgg
     6961 ctggccgcgc gatagaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaaa
     7021 tcagcgggca gatcctgccc gcactgaccg aagtcgccca gcgactggtg cgcgagcagc
     7081 aggttgcagt cccggctgcg cagcgtcccc agcgcgttca gcacgtactt cgacagcaga
     7141 tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca
     7201 atgatgattt gcgcgcagcg ggcaaacagc atcttctgca cccggatcac cgcctcatcg
     7261 tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc cacatcgata
     7321 ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc
     7381 ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg ccgccagcac ctgctgaatg
     7441 tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg
     7501 tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc
     7561 agggcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga
     7621 tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct
     7681 ttggggtcga agtacaccac agcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg
     7741 gtggccatcg cgctcttgcc gctcccgggc aggccgagga tctgaatatt ggtcttgcgc
     7801 caggtatcac gagggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc
     7861 tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc
     7921 ccgaaatgca ggtggcggac gtctgtcatt tctccggtcc gctggctgct gcgccggctc
     7981 tcctgattca gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc atcaagcagc
     8041 aggaacgcca gcagccgcgc cagaaggacg ccggcgacaa gccctccggc acaccaggct
     8101 atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcata gaggtccggg
     8161 cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg
     8221 gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgtcg ccgtgccctg
     8281 accatccgcc cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt ccggttatta
     8341 tcgcgcttat agcgatcggc atactcccgc cgcgccttat ggctcagccc cacaaaacgc
     8401 attcggccgg cccagagttc agccagaccg aaaaacaggc cgataaccag aaaaaaaccg
     8461 gccagactgt aatactcccg gtacagccag ctgacccacg ggtaaatctg ccgggccagc
     8521 tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc
     8581 agacgacggg tttaccggcg tgatgcgcag cccccgctgc acaagccgcg gacatgcccc
     8641 aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc
     8701 agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca
     8761 gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc
     8821 agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg
     8881 tcaggtgcgt ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc
     8941 gggtgaattt ctggcgtggc cggcgtccag ccggacagag ccagaaagag gggaaaagac
     9001 cgtgcgtcgg tggcgtccag ccagcgtaag caaggtcttt tcccctgaag tcagccacgc
     9061 cccagcgggg atgacttgca tttctccgca aggagaaaaa agcaacagag ataggttaac
     9121 gtaaaatgtt cagcgaaaaa ccgacatggt tcacctatcc tgataattga tcgtcaggga
     9181 atagaaagac ttaatcaggg gacaatatcc cacatcagcg cgtccggtga ccgtcttgca
     9241 ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggg cgtgagactg
     9301 cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact
     9361 aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt tttccatagg ctccgccctc
     9421 ctgccagagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc gtgaggctca
     9481 accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg
     9541 gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc
     9601 gccgttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc
     9661 tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac
     9721 tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cgggaaagca cgcaaaacgc
     9781 cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg
     9841 gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt
     9901 gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca
     9961 aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag
    10021 gatctcaaga agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga
    10081 ggacgatggt gccaccgttc cgccctgaac atcccggata taatttgcct gacatgcggc
    10141 cagaagaaag ccagcaacaa tcaatataaa aagaaaaata gtctttacct ttttcatata
    10201 ctttccctgt atttaaagac tccggcattc atccctgaac accggaagtc agtatttcaa
    10261 atcatccttg ataatttttt gttaccagtt accgtcccga taatcaaaag ctatataaca
    10321 attatccaca tcaggtttta tttgattttt aaaaaatggc tgtagtaaag gaacccattc
    10381 tttcttcaaa tcaaactcac cattattaat attatccttt aaaggcattc ccaagctttc
    10441 aatgacagaa ccatcgtcac caaagtcttt agaatactct tcccctttaa actcttcagt
    10501 tttcttatca aaccatgtca aacgtaattt aagccccata tttacctctc aaagatattt
    10561 tttaatgttc cgttttggat ctggcccttt aacctgctta cccgttttgg gatcaaatgc
    10621 gcctaggtgt tcgccatcac tggcacgata cccttcaagc tcaccgtgct gggaatccca
    10681 ttcataaatt ttacgttttt tatcgccata ccatcgggcc cgcttaccac caccaccttg
    10741 tttaggtgtt ttaggatcac ctcgctttaa ctctccaagt ccctttatgt cttctgtttt
    10801 aggtactgga tgataatcat ggccgtagtc tttagtcccc ttgcgaggct tgttcttttc
    10861 ctcatttaac ttattttctg catccttcgc ttttttctcc ttttgtttcc ggctctccat
    10921 cgccccggta agtgcagcct tatctctgga taaggattca ttcattgcgt ttaaagatgc
    10981 ctgagccgct tttaatttct cattctgatt ctgaatatcc ttatcaagat agcgaacctg
    11041 atcctgccac ccggagccat attcatgagg cgggttataa gtcacaaaat catctttatt
    11101 tgcctctgcc tcttttacct ttttatcggc ttcctgaaca gcagcccttg caccggggag
    11161 cgccttctct gtcgatgcaa tgcggctgtt taaggtcgca atatttttat cttcggcatc
    11221 cagctcagct ttcgctttat catagtctct tttcagcccc tcttccggat gagcggcgtc
    11281 ccatgcctgc tggcgacgct tttcttcctc ctgacgctgt tttacctgtg ccggggtaag
    11341 aacatctgtc accgacacat aaaccggctt ctgtccggtc tctttcggga aacgaataac
    11401 ggcctcacgg gagttaccac cggctgtaaa accagccgga cgtgaatcac ctttttcagc
    11461 aacaatgcct tttggcgggg ctttcgctgt cggaacacct ttcggtacgc tcacctgcag
    11521 agacgggaga cccggaatcg acacactgaa tacccccgga cgttttgtcg gtttcgcatc
    11581 caccacaggg acactcattg gccgacctgc gacaaccgca atatgctgcc gctcatcctt
    11641 caccacatcc acaacccgtt gtctgacacg aaccgtcgcc tggtccagtg gtaaagtact
    11701 tgccggagtc tccgtcaccg catcggccgg taatgacgtc ataatttttg acatcatttt
    11761 cggatcatct tttgcaatct cagaaggcag cacaccgtag atcgcaatcc cccacagacc
    11821 aaacttaaac ggccccttca gggcagccag cacatcagca acggcggccg acaacgcatc
    11881 gccggaaact gataaagcca gtccttcagc ccccggagta gccagggcag gcagaccgaa
    11941 ggccatcgca gtggccgaag actgtccgcc
//
