LOCUS       NZ_CP102075             3348 bp    DNA     circular CON 20-AUG-2023
DEFINITION  Enterococcus faecalis strain JF3A-4253 plasmid pJF3A-4253-6,
            complete sequence.
ACCESSION   NZ_CP102075
VERSION     NZ_CP102075.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30011847
            Assembly: GCF_024579795.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Chae,J., Oh,J. and Jeong,J.
  TITLE     Enterococcus faecalis JF3A-4253 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Chae,J., Oh,J. and Jeong,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2022) Division of Biotechnology, Jeonbuk National
            University, Kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.9.4b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.0x
            Sequencing Technology :: Illumina iSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/20/2023 05:03:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,927
            Genes (coding)                    :: 2,882
            CDSs (with protein)               :: 2,882
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 31 of 45
            Pseudo Genes (internal stop)      :: 6 of 45
            Pseudo Genes (multiple problems)  :: 3 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102075.1.
            Bacteria and source DNA available from Bacteria.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="JF3A-4253"
                     /isolation_source="Rectal swab"
                     /host="Pig"
                     /db_xref="taxon:1351"
                     /plasmid="pJF3A-4253-6"
                     /geo_loc_name="South Korea"
                     /collection_date="2020"
     gene            join(2481..3348,1..380)
                     /gene="mobV"
                     /locus_tag="NP948_RS14980"
     CDS             join(2481..3348,1..380)
                     /gene="mobV"
                     /locus_tag="NP948_RS14980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_256969085.1"
                     /translation="MAHLKKNTRASLSGLSIHFERKTDNHSNKEIDISRSHLNQDLMQD
                     NSNMVERFNERLEDVYCMNRKDVKALGTWVVTLPDELKDLSHDKQQEFFNETKNFLDKR
                     YGKENAVAAIVHYDETTPHLHYAFVPVTFDEKKDRLKVSAKEVLNRKDLQTFHDDLNKH
                     LKEKLPFYEKGILNDQTLPFQNVEEIKKYNETYQEMKQALEQKKAVIRLKNDALEPFEK
                     SLKRINDTEKMFDTIDKNAKKGFGQVVIKSSDFEKLKTQAIENQVLANANEKEMYILKD
                     KNKKLEKDNRMLSTKLDHKTQKNEALTKKNNELTKVKNDGHKFRELVKRDLEKQYNIVD
                     LTEKEVNALSVVKAIDEDIKPKNKEEGKSWLDDLNNGKGTRIEESMLERALEKALELYR
                     EIVQKVERMMGMNISL"
                     /name="mobV"
     gene            967..1938
                     /locus_tag="NP948_RS14985"
     CDS             967..1938
                     /locus_tag="NP948_RS14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_159157046.1"
                     /translation="MSKNGTIVNEWEKLTALSNRRLNAQTMCNPLKMQWSIDRITIVGT
                     LRKNIYYHTTHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRSSERQIRMYNKKLEQERKRKIVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDKMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEKE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1952..2170
                     /locus_tag="NP948_RS14990"
     CDS             1952..2170
                     /locus_tag="NP948_RS14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025480940.1"
                     /translation="MKDVQDLFKEYYDSHNLEKNSQYADFSKEQLVIEAEYLHDSLTRI
                     LKYINDGGTDINKIYAEVMDGIYESRI"
                     /name="hypothetical protein"
     Typing          1051..1929
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.126"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atcgtatgtt gtctactaaa ctagatcata aaacacaaaa aaatgaagca ttaactaaaa
       61 aaaataatga attaacaaaa gttaaaaatg atggtcataa atttagagaa ttggtgaaaa
      121 gagatttaga aaaacaatac aatatagttg atttaacaga aaaagaagtg aatgctttgt
      181 ctgttgtaaa agcaattgat gaagatatta agccaaaaaa taaagaagag ggtaaaagct
      241 ggttagatga tttgaacaat gggaaaggca cgagaataga agaatcaatg cttgaaagag
      301 cacttgaaaa agcacttgaa ttatatagag agattgttca aaaagtggaa cgtatgatgg
      361 gcatgaatat atctttataa agcccataaa atgcgttata aggcgttttg attcttatgg
      421 gtgtatttct attgttttgt ttttaaaaag cctgtataag cctaaaaatg tgttctaagg
      481 cgttttagcg tttatttcgt ttagttgtcg gcataatcgt taaaacagag gttctcgtaa
      541 cgtaaaagcc cttgagcgta gcgtggcttt gcagtgaaga ggttcgctgt tagattatga
      601 aagccgataa ctgaatgaaa taataagcga tagcccacta tttcggtagg aagaggctca
      661 agggagtttg agggaatgaa attccctcat gatttatatt tatttttagt acttcttttg
      721 tctttccaaa tttaagtgat atactaaaaa aaacagaaaa aagagcaaaa aaatagccga
      781 gcagtgcgac aacacttctc gactaattac ggaaactact tgtactagct ccgtaactca
      841 caatcattat acgaatattt ttaggtttct tcaagactag agattcttgt taatgaattt
      901 taagggttac gtgaactttt aaaattcttt ccaagaatct cttttttctt tgaggaggaa
      961 cgaaatttga gtaaaaatgg cacgatcgta aatgagtggg aaaaattgac cgccctatct
     1021 aacaggcggt taaatgcaca aactatgtgc aaccctctaa aaatgcagtg gtcaattgac
     1081 cgaattacta ttgttggtac attgagaaaa aatatttatt atcacactac ccacgatgtg
     1141 ttgattttag attttgaaca gcttatgcgc ctaaacgaag gtaacggata tttaaaagcg
     1201 gttggaaata atggctggca attactagac caatatgaag aaaatattgc ttatattgag
     1261 attttgaaat ggcaagaggg aaaagggcgg attgacttta acccaaacaa aattagccaa
     1321 tttctggcgg gttcgatgaa gaactttatc catgatttat ttttagaacc acatttctca
     1381 cgtgcagata ttgcttgcga cattatcgac gtgcctgatg attttattac gcaatatcgt
     1441 gtagttgatc cagtgtcctt caaaccaata tatgggcggt ctggaaagtt agaaacagct
     1501 tattggggga gtcgttccag tgaacgccag attcggatgt ataacaaaaa attggaacaa
     1561 gaaagaaaac ggaaaattgt tccgaaagaa atagatactt ggtggcgtct agaattacaa
     1621 ttgcgaaggg gaaaagcaac tgattggcat gcaatggttc atgaaagttt agacagcttt
     1681 gcaagtcctc actatttgcc agaagatgtg aaagtaactg acaaaatgat gattactgcc
     1741 cttataactg aacatagtta ttggggtcaa attcatagaa attcaaaata taaatatcgt
     1801 gaattattaa agcaagaaag tcaaaatgat gagttgacga atcatttacg agaaacgttt
     1861 tcggaatcgg cagatgtttt gaaaaaagaa ttagatactt ggttgcaagg tttagatgtt
     1921 acagaagaaa aagagtaaag gggatatttt tatgaaagat gtacaagatt tatttaagga
     1981 atattatgat agccataatt tagaaaaaaa tagtcagtat gcagatttta gcaaagaaca
     2041 attagtgatt gaagcagaat atcttcatga ttctttaact cgtattttga aatatataaa
     2101 tgatggtgga actgatataa ataaaatata tgctgaagtt atggacggaa tatatgaaag
     2161 tcggatataa ctttttagga gtaatcgtca aaaggcgatt attctttttt tatattttcg
     2221 gaaaatggca atctcgccaa ctccgaaaat ataaaagcag cgaatgaaaa tttgaaaaaa
     2281 ttttgattgc tgtaaggcta aaaaagagaa gatcataaat tgcctaaagg cgatttattc
     2341 tgaacgtttt agctcgcctg cttgcaggcg aatagctctc ggcagagcct gtccaaatga
     2401 aatttggtat aacaggctat accagatttt agactatgtt atactggttc taaagaccct
     2461 atgaactgga gggatattat atggctcatt taaagaaaaa tactcgtgct tctcttagtg
     2521 gtttatcaat tcatttcgag cgcaaaactg ataatcattc aaacaaagaa atagatatta
     2581 gccgctcaca tttgaatcaa gatttaatgc aagacaactc aaatatggtc gaacgtttta
     2641 atgaaagact agaagatgtt tattgtatga atcgaaaaga tgttaaggca ttaggaactt
     2701 gggttgtaac gttgcctgat gaattaaaag atttatctca tgacaagcaa caagaatttt
     2761 ttaacgaaac gaaaaatttt ttagataaac gatacggaaa agaaaatgct gtcgctgcga
     2821 ttgtacacta tgacgaaaca acaccacact tacattatgc gtttgttcct gttaccttcg
     2881 atgaaaagaa agatagatta aaagtatctg caaaagaagt cttaaacaga aaagatttac
     2941 agacatttca tgatgattta aacaagcatt tgaaagagaa gttgcctttt tatgaaaagg
     3001 gaattttgaa tgaccaaaca ttgccttttc aaaatgttga agaaataaaa aaatacaacg
     3061 aaacatatca agaaatgaaa caagcattag agcaaaaaaa agcagtcatc agattgaaaa
     3121 atgatgcgtt agaacctttt gaaaaatcat taaaaagaat caatgataca gaaaaaatgt
     3181 ttgatacgat tgataaaaat gctaaaaagg gatttggtca agtcgttata aaatcaagtg
     3241 attttgaaaa attaaaaacg caggcaatag aaaatcaggt tcttgcgaat gcgaatgaaa
     3301 aagagatgta tattttaaaa gataaaaata aaaaattaga aaaagata
//
