LOCUS       NZ_CP102074            10905 bp    DNA     circular CON 20-AUG-2023
DEFINITION  Enterococcus faecalis strain JF3A-4253 plasmid pJF3A-4253-5,
            complete sequence.
ACCESSION   NZ_CP102074
VERSION     NZ_CP102074.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30011847
            Assembly: GCF_024579795.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Chae,J., Oh,J. and Jeong,J.
  TITLE     Enterococcus faecalis JF3A-4253 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Chae,J., Oh,J. and Jeong,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2022) Division of Biotechnology, Jeonbuk National
            University, Kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.9.4b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 103.0x
            Sequencing Technology :: Illumina iSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/20/2023 05:03:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,927
            Genes (coding)                    :: 2,882
            CDSs (with protein)               :: 2,882
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 31 of 45
            Pseudo Genes (internal stop)      :: 6 of 45
            Pseudo Genes (multiple problems)  :: 3 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102074.1.
            Bacteria and source DNA available from Bacteria.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="JF3A-4253"
                     /isolation_source="Rectal swab"
                     /host="Pig"
                     /db_xref="taxon:1351"
                     /plasmid="pJF3A-4253-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2020"
     gene            complement(join(9984..10905,1..899))
                     /locus_tag="NP948_RS14920"
     CDS             complement(join(9984..10905,1..899))
                     /locus_tag="NP948_RS14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="WP_256969077.1"
                     /translation="MKKEFTDMKKEFTELYDFIFDPIFLVRYGYYDISIPNKKMNTEKV
                     EIDNDYGKSDSFYFKVFNMESLANYLRSHDLKKHFNGNKPLSTEPVYFNIPKNIEARRQ
                     YKMPNLYSYMALNYYICDNKKEFIDVFMDNKFSTSKFFNQLSFNYPKTQEIRQTLLYGG
                     IKKLHLDLSNFYHTLYTHSIPWMIDGKSTAKKNRKKGFSNRLDTLITACQYEETHGIPT
                     GNLLSRIITELYMCHFDKQMERKNFVYSRYVDDFIFPFTLENEKQEFLNEFNLICRENN
                     LLINDNKTKVDNFPFVDQSSKSDIFSFFENITSMNSNDKWIKEISDFIDYCVNEEHLGN
                     KGAIKCIFPVIKNTLKQKKVDTKNIDIIFSKRNMVTNFNVFEKILDLSLKDSKLTNKFL
                     TFFENINEFGFSSLSASNIVKKYFSNNSKGIKNKIDHYRKNNFNQELYQILLYAVVFEI
                     DDLLNQEELLNLIDSNIDDYSLILGTILYLKNSSYKLDKLLKKIDSLFINTHANYNVNT
                     SRMAEKLWLFRYFFYFLNCKDIISKIEINSYCKSKKYKSGPNGYQTELNWNYIKGQGND
                     LRANDFFNELILAEVWLIYCGENEDFKYLN"
                     /name="RNA-directed DNA polymerase"
     gene            1127..1342
                     /locus_tag="NP948_RS14925"
     CDS             1127..1342
                     /locus_tag="NP948_RS14925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256969078.1"
                     /translation="MPYHIFINRESGNDNISIFYDEPTFVVIANKDDLPAIQLIEESQK
                     PGIYILLGDNKRYVGQASNSIFSRLQ"
                     /name="hypothetical protein"
     gene            1451..1960
                     /locus_tag="NP948_RS14930"
     CDS             1451..1960
                     /locus_tag="NP948_RS14930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256969079.1"
                     /translation="MNTANIDLDNSTQGNKSYIDKLSKFSALSLWNKVEKLLNDVANID
                     LFDSEHIQTEDNSDNTTLNSHISIIFGESLYTGRSYRDVFTQLVSNLVLSAELDKLIPL
                     MSPNEPNTVQILGNREHISAKGTKLTKPIELTKYHMYINFSKIGLYNQIKKLAELTGKK
                     VIFERW"
                     /name="hypothetical protein"
     gene            1966..2556
                     /locus_tag="NP948_RS14935"
     CDS             1966..2556
                     /locus_tag="NP948_RS14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_256969080.1"
                     /translation="MVQQIVLPIKDSNILKMVQDTLLDSFRAGRRNYTIFQVGKATLLR
                     VSDVMKLKKSDVFNPDGTVKQTAFIHDQKTGKANTLYLKPVQQDLLMYHDWLVQQNMNS
                     EWLFPSTARPDRHITEKQFYKVMAHVGDLLGINYLGTHTMRKTGAYRVYTQSNYNIGLV
                     MHLLNHSSEAMTLAYLGLDQASRENMLDQIDFG"
                     /name="site-specific integrase"
     gene            2688..2972
                     /locus_tag="NP948_RS14940"
     CDS             2688..2972
                     /locus_tag="NP948_RS14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="WP_172685311.1"
                     /translation="MDEQTKYERFFVRSSMVLGKRGTIFVLRNRPSFSSFCMTKRKNFC
                     PFYFYKMGNWRLRKANRHNPKGIKGESETSPFSSHIVIQERKVLLLMIG"
                     /name="cytochrome B"
     gene            complement(3359..3868)
                     /locus_tag="NP948_RS14945"
     CDS             complement(3359..3868)
                     /locus_tag="NP948_RS14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_256969082.1"
                     /translation="MLGKNIKALRKSKGLSQEELAVKLNVVRQTISKWEQGLSVPDANI
                     LISISEEFETPVNTLLGETIIESKADDLKVISEKLEVINLQLAQRKATRRKIFHCFFIL
                     LLIVIMAIFTILLVANSPYLDWDYSDPENAVVGVILHGFEWVFIRIAPIALVGVILGIF
                     LTREKG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4155..4637)
                     /locus_tag="NP948_RS14950"
     CDS             complement(4155..4637)
                     /locus_tag="NP948_RS14950"
                     /inference="COORDINATES: protein motif:HMM:NF016294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_256969083.1"
                     /translation="MKEKIYTIKNLCNELNQPRQKVRRRIEKLDIKAINEDTRIHENEP
                     LEYDHQAFLKLAKEFDVQINNTECTASVQHRTAEETSKDKLIKVLENQLEEANKSRANL
                     EKLLDQQQQLTLVSNKKIESLRLELEERESEKQIDEEINKEEMKKDEKKKWWQFFR"
                     /name="DUF536 domain-containing protein"
     gene            complement(5108..5371)
                     /locus_tag="NP948_RS14955"
     CDS             complement(5108..5371)
                     /locus_tag="NP948_RS14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epsilon-antitoxin"
                     /protein_id="WP_002336758.1"
                     /translation="MTLDYRKTFEIEIINEFQSAIHSKMLNYVLNNELDKSDSTNLQVN
                     LLKQLSNMNQINLFELSLEELEAYHDYLRTIKKYADNITRTA"
                     /name="epsilon-antitoxin"
     gene            5942..6544
                     /locus_tag="NP948_RS14960"
     CDS             5942..6544
                     /locus_tag="NP948_RS14960"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_002410828.1"
                     /translation="MVLDNKLGLTNSAELAKQEEILTKKRAKELFESGKIEDLEIGTFQ
                     GLSDIHQFLFQDIYDFAGKIREVNIAKGNFQFAPRIFLAQTLEYIDKLPQETFDEIIDK
                     YSDMNVAHPFREGNGRATRIWLDLILKNKLHKIVDWNQIDKDEYLNAMIRSTVSTNELK
                     YLIQKALTDDLGKEQFFKGIDASYYYEGYYEIKTEDL"
                     /name="protein adenylyltransferase Fic"
     gene            6942..8108
                     /locus_tag="NP948_RS14965"
     CDS             6942..8108
                     /locus_tag="NP948_RS14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252619.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_256969074.1"
                     /translation="MSIIPEKQENQKQVLTLNELSKRKVVEHNSLITSIAKMDKTPLKM
                     FELAVSCINTEEPPKDNTVYLSKRDLFAFFKVSDNDKHSRFKQAVEKMQKQAFFQIKEE
                     AGKGFKFKSIVPIPYVEWTDYNDEVKIEFHREIMPYLINLKKNFTQHALSDIAELNSKY
                     SIILYRWLSMQYNQYEHYSVKGGRRAEQVEAYRNPSISIKELRIMTDTVNSYKQFTRFN
                     NDVLKTPLDEINAHTSFNVTYEKVKKGRSIDSIVFHITKKPVARNDFYKLEEQDPIYLQ
                     DKANKEQAEELLAGKALKSKYTKLLLDNMLLSPYEMTDTSLMAGLQAHVYPLYDELKAL
                     RGLNGVKDHLSYVRAKQEAYSKRNIAKYLKKAIEQYLPTVKRQDIENE"
                     /name="RepB family plasmid replication initiator protein"
     gene            8101..8676
                     /locus_tag="NP948_RS14970"
     CDS             8101..8676
                     /locus_tag="NP948_RS14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256969075.1"
                     /translation="MNENYKTIKELADELGVSKSYVDKIIRILKLHTKLDKVGNKYVIS
                     KKQEKAIITRIEKPKTTTESKTKLHTKVNSEVDFLKEEIAYLKSNHDKQLTSKDKQIET
                     LSNLLDQQQRLALQDKKLLDEYKSEINDLKALKMPSQETEFKHLDNQYKDEVNALKEKL
                     ENLQEQIKDQKRIEEQEKPRKWWGLWRK"
                     /name="hypothetical protein"
     gene            8664..9872
                     /locus_tag="NP948_RS14975"
     CDS             8664..9872
                     /locus_tag="NP948_RS14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_256969076.1"
                     /translation="MAKIGDSVKKKVPELRFPGFTDDWEECKLGEFSDVRDGTHASPKY
                     VSQGHPMVTSKNLTHSGLDMTDVSFLTDKDFNEINKRSKVSIGDILFGMIGTIGNPVIV
                     DRDDFAIKNVALIKEKTSNPITNKWLLQYLKSPSFNRFIQKENAGGTQKFIALGLIRDM
                     KLRVPEFDEQEKIGAFFKQLDDTIALHQRKLDLLKEQKKGYLQKMFPKNGEKVPELRFA
                     GFADDWEQRKLGEIVQITMGQSPNSENYTENPFDHILVQGNADMKNNRVVPRVWTTQIT
                     KQAEKGDLILSVRAPVGDIGKTDYDVVLGRGVAAIKGNDFIFQQLGKMKEEGFWNRFST
                     GSTFESINSNDIKEALITIPNEEEQKRIGNFFARIDQAITLHQRKLDLLKEQKKGFLQK
                     MFI"
                     /name="restriction endonuclease subunit S"
     Typing          6943..8092
                     /name="rep_cluster_554"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.505"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gattgatcaa caaaagggaa attatcaact tttgttttat tatcattaat aagtaagttg
       61 ttttctcgac agattagatt aaattcattt aaaaattctt gcttctcatt ctcaagagta
      121 aacggaaata taaaatcatc cacatatctc gaatacacaa aattcttacg ttccatttgt
      181 ttgtcgaaat ggcacatata tagttcggta ataatcctag acaataaatt tccagttgga
      241 attccatgtg tttcttcata ttgacaagct gtaatcaaag tatctaatct gttagaaaat
      301 cctttttttc tatttttttt agctgtagat tttccatcaa tcatccatgg tatactatgt
      361 gtatataaag tatgataaaa attagataaa tctaaatgta atttttttat tcctccatat
      421 aataatgttt gtctaatttc ttgtgtctta ggataattaa aactcaattg attaaaaaat
      481 tttgacgttg aaaatttgtt atccataaat acatcaataa actctttttt attgtcacat
      541 atataataat ttaatgccat ataactgtat aaattaggca ttttatattg tcttcttgct
      601 tctatatttt ttggaatatt aaaatatacc ggttctgttg atagcggttt gttgccatta
      661 aaatgttttt ttaagtcatg acttcttaaa tagtttgcca aagattccat attaaatact
      721 ttaaaataaa aagaatctga ttttccataa tcattgtcta tttcgacttt ttcagtattc
      781 atttttttat ttggaataga tatatcataa tagccatatc ttacaagaaa aataggatca
      841 aatataaaat catataattc cgtaaactct tttttcatat ccgtaaactc ttttttcata
      901 attaaacccc tttaaattct atagttaata attcaattct atttcaaatt ataataaaaa
      961 aatagttatt ttatattagt gaagtatgat atacaaaaag ataaggtcta taattagata
     1021 ataaccccta agaaatcaaa taaaaacagc ccctataatc taaattctag attatagggg
     1081 ctgtttattt attttaatga tataataacc ctaaaaggag gacgttatgc cataccatat
     1141 atttattaat cgagaatctg gaaatgataa tatttctatt ttttatgatg aacctacttt
     1201 tgtagttatt gccaataaag acgatttacc agcaattcaa ttaattgagg aatctcaaaa
     1261 accaggtata tatatattgc taggagataa taaacgttat gtaggtcaag cctctaattc
     1321 tattttttct aggttacaat aacacaatac aaataaacca tggtggaaca aattaatatt
     1381 ttttggacgt gaagatgggc atctatctaa agcacaacta gatcttttag aacgtttaat
     1441 cattgaaaaa atgaatactg ctaatattga tttagataat agcactcaag gaaataaaag
     1501 ctatattgat aaattaagca aattttcagc tctgtcatta tggaataagg ttgaaaaact
     1561 attgaatgat gttgccaata tcgatttatt tgattcagaa cacattcaaa ctgaagataa
     1621 tagtgacaac acaactctaa atagtcatat atcaatcata tttggagaat cattatatac
     1681 tgggagatca tatagagatg tatttacgca attagtttct aatttagtat tatccgcaga
     1741 gttggataaa ttgattcctt taatgagtcc taatgaaccc aatacagtac aaatacttgg
     1801 caatagagaa catatatcag ctaaagggac gaagttgact aaaccaatag aactaactaa
     1861 ataccatatg tatattaatt tttcaaaaat aggcttatac aatcaaatca aaaaattagc
     1921 tgaactgact ggaaaaaaag ttatatttga aaggtggtaa ttcatatggt tcaacaaatc
     1981 gttctaccca tcaaagattc aaatatctta aaaatggtgc aggatacact acttgatagt
     2041 tttcgtgctg gtcgtcgcaa ctatacgatc ttccaagttg gaaaagcaac gcttctacgt
     2101 gtgagtgacg tcatgaagct aaagaaatca gacgtattca atcctgacgg aacagtaaaa
     2161 cagactgctt tcattcatga tcaaaagaca ggtaaagcga atactttgta tttaaaacct
     2221 gtccaacaag atttattgat gtatcatgat tggctggtcc aacaaaatat gaattctgag
     2281 tggctatttc cttcaactgc tcgtcctgac cgtcatatca cggagaaaca attttataag
     2341 gtcatggcgc acgttggcga tcttttaggt atcaactatt taggaacaca taccatgcgt
     2401 aaaacaggcg cttaccgtgt ttatacgcag tcaaattata atattggttt agttatgcat
     2461 ttattgaacc attccagtga agctatgact ttagcttatc tcggtttaga tcaagccagt
     2521 cgagagaata tgttagacca aattgatttt gggtaatgaa tgtttagaat ttagtagtcg
     2581 tccaaaaacg actacttttt ttgttaaatc gtccaaggac gatttaatgc ttttgacttg
     2641 taagaacagg attgattgaa tgaagccata ctgttcgcat atttttagtg gatgaacaaa
     2701 caaaatacga gagatttttt gttcgttcat ccatggtttt aggaaaaaga gggacgattt
     2761 tcgtcttacg aaatcgtccc tctttttctt ctttttgtat gacaaaaaga aagaactttt
     2821 gcccatttta tttttataaa atgggcaatt ggcgtttgcg taaagcaaat cgacacaatc
     2881 caaaggggat aaaaggggaa agtgaaactt cccctttttc aagccacatt gtaatacaag
     2941 aacgaaaagt attactactc atgattggtt gaatggaggc aaatgaaata aaattcaaat
     3001 acatgagcga agcgaatttt aataaacacg cttgaatgtc ctgcaactgg ctgtcagttg
     3061 caggacgtgt gaaagatagt cagtatctaa attttaataa atttagatac atttatttgt
     3121 tatactttag atcaattaat tgaacactcc atggcgtaaa catgaatagt gaaaatgcaa
     3181 taagaaaaat acttataaaa agagtagcct tgttagcttg attattttta cgagcagata
     3241 tccaacgaaa caaaagaaaa ataccaacaa gtaatgttaa aaagtaccaa aatcccatct
     3301 gaaacactcc tttttaattt tttaaattat acgatccatt ccttaaataa gacctgtttt
     3361 aacctttctc tcgtgttaaa aaaattccga ggataacgcc aacaagagca atcggggcta
     3421 ttcgaataaa cacccactcg aatccgtgta aaatcactcc aacaactgcg ttttcagggt
     3481 cactataatc ccaatccaag taaggactat ttgccacaag taaaattgta aaaatagcca
     3541 ttataactat gagtagtaaa ataaaaaaac aatgaaaaat tttccgtctt gttgcttttc
     3601 tttgtgcgag ttgcaaatta ataacctcaa gtttttcaga aattactttt agatcatcgg
     3661 ccttcgactc aattatcgtt tctccaagca aagtgtttac gggtgtttca aactcttccg
     3721 atatggaaat aagtatatta gcatcgggaa ccgataatcc ttgttcccat ttagatattg
     3781 tttgtcgtac cacattaagt ttgacagcaa gttcttcttg cgaaagtcct ttcgattttc
     3841 taagtgcttt aatatttttt cctaacatga gtgatcacct cttttctact gttagtatat
     3901 aattaattac gcgttgctat aagcaatgta aattaacatt gaattgaaaa aagcaacaat
     3961 cctcttcaaa acaagaattg ttgctttttt atgaaaatta ttgtttctaa cgatcattct
     4021 aaggtgtgtt taagctgctt agctacatat tcctatcgat ccattgtaca aaagctataa
     4081 aagtacctct ttaatttgtg atagtagttc agtttctact ttattatcag acattacatc
     4141 attctccttt atatctatct aaaaaattgc caccacttct ttttttcatc ttttttcatt
     4201 tcttctttat ttatttcttc atcaatctgt ttttctgatt ctctttcttc aagttctaat
     4261 cttaatgatt caattttttt attagaaacc aaagttaatt gttgttgttg atctaaaagt
     4321 ttttctaaat ttgctcttga tttatttgct tcttctaatt gattttctaa tacctttatt
     4381 aacttatctt tacttgtttc ttctgctgta cggtgctgta cacttgctgt acactctgta
     4441 ttatttatct gcacatcaaa ctcttttgct aatttcaaaa aagcttggtg atcatactcc
     4501 agaggttcat tttcgtggat tctcgtatct tcattaattg ccttgatatc aagcttttca
     4561 atccttcttc ttaccttttg tcttggctga tttaattcat tacacaagtt ttttattgta
     4621 taaattttct ccttcatatg ccacctcact tataaatcat tgtatcataa tactgtacaa
     4681 atcgttgtac gctgttgtat caagatggtt ttagacgaga gaagaaagtt aatagggaga
     4741 caggtgaaat tacgaatgat ttcgagggct gataaacttg agttataaag atttttttga
     4801 taaataattt tagacaagag cgaagcgaat tttaacaaac acgcttgacg aaaaaagagt
     4861 agagtttcac gtaagtgatc tgtactcttt tttagcacaa acctatcaat ttttcgtaag
     4921 aaaaatttgg tgggtataag tgcgccctta gtaaaaatcg ctaagggaat atttttcaaa
     4981 ttaattttgt agaaacgcac cccttgtttt taagggcgca taaatacgca gtaacgaagt
     5041 tactgcgtat ttatgcgata aaacttaact gttagttagt tttatcgtca ggcgtgttgg
     5101 ttaaaactca cgctgttcgt gtgatgttat cagcatattt ttttatcgtt cgtaaatagt
     5161 cgtgataggc ttctaattct tctaaagata attcaaataa attaatttgg ttcatgtttg
     5221 ataattgttt taataaattt acttgtaggt ttgtcgaatc acttttatcg agttcattat
     5281 ttaaaacata gttcaacatt tttgagtgaa tcgctgattg aaattcattg attatctcaa
     5341 tttcaaatgt ttttctgtaa tctaatgtca tgttgtttgc tcctttctgg tttcgctttg
     5401 ctcaactaaa catttacttt taattaaaca gacttcgcta gtttaatcaa aagtaaacgt
     5461 acgggtaaaa aacaattttc tcaaaaaacg tcataaaatt cgttttaagg cgtttttcat
     5521 attaaacgtc tatttattca aatgtaagat taaaacgcct tagaaacgat tttaagtggg
     5581 tttaaattga ttttaaatga actgttttgt ttggaattaa aaaacaatta ttcatgtagg
     5641 tttttaattc aagggtcgtt agggcttgcc ctgaccgcct gtaaggcgtt tgattgccag
     5701 ataggcgtat ttatctagtc aaacaattaa aacagcgtat acgagcaatt agagccaatc
     5761 aagaaaatcc ctaaaagagg gtttgatctt ttcttttagc gagtgaacga agtgaacgct
     5821 gcaagttaaa tgtgagcgtg gctttcgctc actcctttag gtataatatt aggtgtagaa
     5881 actactgaat gagaacaaat caagtgggca ttttatattt aatagatgat ggggtgatac
     5941 cgtggtttta gataataaat taggtttaac aaattcagca gaattagcca aacaagaaga
     6001 aatcttaacg aaaaaaagag ccaaagaatt gtttgagtct ggcaaaatag aggatcttga
     6061 aattgggacg tttcaaggct tatctgatat tcatcagttt ttattccaag atatttacga
     6121 ctttgcagga aaaattcgag aagtgaatat tgcgaaagga aactttcaat ttgcgccacg
     6181 tattttttta gcgcaaacac ttgaatatat tgataaatta cctcaagaaa catttgatga
     6241 aattattgat aagtattcgg atatgaatgt agcgcatcct tttagggaag gcaatggacg
     6301 agccactcgt atctggttgg atttaattct taaaaataaa ctacacaaaa tcgtcgattg
     6361 gaatcaaatt gataaggatg aatatttaaa tgccatgatc cgtagtacag tcagcaccaa
     6421 tgaattaaaa tatttgattc aaaaggcttt aaccgatgat ttaggaaaag aacaattctt
     6481 taaaggaatt gatgcgagtt attattacga gggctattac gaaattaaaa cagaagatct
     6541 ataaacacgt ataaacacga tttaaagaaa cagactgctc aatataaaac ttgagcagtc
     6601 tgtttctttt tttggatgat tttgaccttt ggttttaaat ttttaaaaaa aataaaaaat
     6661 aggcgaagcc tatattacat ttatcttata tattttaatc ttttcttctt ttgcgtgaaa
     6721 aaaaagtcag tgtttaaagg tggatacaga attatagcgt atgaaaaaac tgtttatagc
     6781 gtatgaaaaa actgtttata gcgtatgaaa aaactgttta tagcgtatga aaaaacttga
     6841 tggatatacg gtattctgat ataataaaag catagagaaa tcgacagcga aatcagtttc
     6901 tctatgccta cttaaaatca ctcacaaagg agtaactttc tatgtctatt ataccagaaa
     6961 aacaagaaaa ccaaaagcag gtgctgacct tgaatgaact gtcaaaaaga aaagtggtag
     7021 agcataatag cttgattacg tctattgcta aaatggataa aacgccttta aaaatgtttg
     7081 agttagccgt gtcttgtatc aataccgaag agccaccgaa agacaatacg gtttatcttt
     7141 caaaaagaga ccttttcgct ttttttaaag tttccgacaa tgataagcat agtcgcttta
     7201 aacaagcggt tgagaaaatg caaaaacaag ccttttttca aatcaaagaa gaagcaggca
     7261 agggttttaa attcaaaagc attgtgccta tcccttacgt agaatggacg gattataacg
     7321 acgaagtaaa aattgaattt catcgtgaaa ttatgcctta cttaatcaat ctcaaaaaga
     7381 attttaccca acacgcttta tcagatatag ccgaactcaa tagcaagtat tcaattattc
     7441 tctaccgttg gttatctatg caatacaacc aatacgagca ttacagtgtc aaaggcggac
     7501 ggcgagccga acaagtggaa gcataccgca atccgtctat ttcaattaaa gagttgcgta
     7561 tcatgactga taccgtaaat tcttataaac agtttacaag atttaacaat gatgttctta
     7621 aaacaccttt agatgaaatt aacgcccata cgtcttttaa cgtgacctat gagaaagtca
     7681 aaaaagggcg ttcgattgat tctattgtct ttcatatcac gaagaaacca gtcgcacgaa
     7741 atgacttcta caagctggaa gaacaagacc ctatttattt gcaggataaa gcaaataaag
     7801 aacaagcaga agaactttta gcagggaaag cattaaaaag taaatacacg aaacttttgt
     7861 tggataatat gctattaagt ccttatgaaa tgaccgatac gtcacttatg gcaggtttgc
     7921 aggcgcacgt gtaccctctc tatgacgagc tgaaagcctt acgagggttg aatggggtca
     7981 aagaccacct ctcttatgtg agagctaaac aagaagccta ttcaaaacgc aatatagcta
     8041 agtatctcaa aaaagcaatt gaacagtacc taccaacggt taaaaggcag gacatagaaa
     8101 atgaatgaaa actacaaaac cataaaggaa cttgcggacg agttaggggt aagtaaatca
     8161 tatgttgata agataatacg catactcaaa ttgcatacta aattagataa agtaggtaat
     8221 aagtatgtga tttctaaaaa gcaagaaaaa gctataataa caaggattga aaagcctaaa
     8281 acaacaactg aatcgaagac taaattgcac actaaagtta attcggaagt tgattttttg
     8341 aaagaagaaa ttgcatatct taaaagcaat cacgataaac aattgaccag caaagataag
     8401 cagatagaaa ctttaagcaa tctcttagac caacaacaac gcctcgcctt gcaggataaa
     8461 aagttacttg atgagtataa gtcagaaatc aatgacttaa aagcccttaa aatgccctca
     8521 caggaaacag aattcaaaca cttagacaat caatataaag atgaagtgaa cgctcttaaa
     8581 gagaagttgg aaaatttgca ggaacaaatc aaagatcaaa aaaggataga agaacaagaa
     8641 aaaccaagaa aatggtgggg actatggcga aaataggtga ttcagttaaa aagaaagttc
     8701 cagaattaag gtttccggga tttacggatg attgggaaga gtgtaagttg ggggaatttt
     8761 ctgatgtaag agatggaaca catgcttctc caaagtacgt tagccaagga caccctatgg
     8821 taacctctaa aaatttaact cattctggtt tagatatgac agatgtatca ttcttaacgg
     8881 ataaagattt taatgaaatt aataagagat caaaggttag tattggagat attttattcg
     8941 gaatgatcgg aactatcggt aatccagtaa ttgttgatag agatgacttt gctattaaaa
     9001 atgtggcact aattaaagaa aaaacttcta atcctataac gaataaatgg ttactgcaat
     9061 atcttaagtc tccatctttt aatcggttta ttcaaaaaga aaatgcaggt gggactcaaa
     9121 aatttatagc acttggtcta attagggata tgaagttaag agttcctgaa ttcgatgagc
     9181 aagaaaaaat aggagcattc tttaaacagt tagatgatac tatcgctctt catcagcgta
     9241 agttagattt gttgaaagaa cagaaaaaag gctacttgca aaagatgttc ccaaaaaatg
     9301 gcgagaaagt tcctgaattg cgatttgcgg ggtttgctga cgattgggaa cagcgtaagt
     9361 tgggtgagat agtacaaatt actatgggtc aatctccaaa ttcagaaaat tatacagaaa
     9421 atccatttga tcatatcttg gttcaaggaa atgccgatat gaaaaataat agagttgtac
     9481 cgagagtttg gactacgcaa attacaaagc aagcagaaaa aggtgatttg attttaagtg
     9541 ttcgcgctcc agttggtgat attggaaaaa ctgattatga tgtagttcta ggacgtgggg
     9601 tcgcagcaat taaagggaat gattttattt ttcagcaact tggaaaaatg aaggaggaag
     9661 gattttggaa tagatttagt acgggctcaa catttgaaag cattaactca aatgatatta
     9721 aagaggctct aatcactatt ccaaacgaag aggaacaaaa aagaattggc aactttttcg
     9781 ctcggatcga tcaagccatc acccttcatc aacgtaagtt agatttactc aaggaacaga
     9841 aaaaaggctt tttacaaaaa atgttcattt aaaatgataa attccaaaat aatttttaaa
     9901 aatagttgtg tcaggtcaat ataaattacc tgacacaact attttgtttt tttgagtcac
     9961 taaaaataga tttcaaatac ttatcaattt aagtatttga aatcttcgtt ctcaccacaa
    10021 taaattaacc aaacttctgc taatattaat tcattaaaaa aatcatttgc tctaagatca
    10081 ttcccttgcc ctttaatgta attccaatta agttctgttt gatatccgtt aggtcctgat
    10141 ttatattttt tagatttaca ataactattt atctctattt tactaataat atctttacaa
    10201 tttaaaaaat aaaagaaata acgaaatagc cataattttt ctgccatacg agaagtgtta
    10261 acgttatagt tagcatgagt attaataaat aagctatcta ttttttttaa taatttatcc
    10321 agtttatatg aactattctt tagatataaa atcgtgccta aaattaaaga ataatcatca
    10381 atatttgaat caattaaatt tagtaattct tcctgattta ataaatcatc tatttcaaag
    10441 acaactgcat acaacaatat ttgatatagt tcttgattga aattattttt acgatagtgg
    10501 tctattttgt ttttaatacc ctttgaatta ttactaaaat attttttcac aatatttgaa
    10561 gctgataaac ttgaaaatcc gaattcatta atattttcaa aaaaagtcaa aaacttatta
    10621 gttaattttg aatcttttaa tgataaatct aatatttttt cgaaaacatt aaaattggta
    10681 accatatttc ttttagaaaa gataatgtct atatttttag tatctacttt tttttgtttc
    10741 aatgtatttt ttatcactgg gaaaatacat tttatagccc ccttatttcc taagtgttct
    10801 tcattaacac aataatctat aaaatcgctt atttctttaa tccacttgtc atttgaattc
    10861 attgaagtaa tattttcaaa aaaagaaaaa atatccgatt tactc
//
