LOCUS       NZ_CP102069             3788 bp    DNA     circular CON 20-AUG-2023
DEFINITION  Enterococcus faecalis strain JF3A-223 plasmid pJF3A-223-5, complete
            sequence.
ACCESSION   NZ_CP102069
VERSION     NZ_CP102069.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30011706
            Assembly: GCF_024579755.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Chae,J., Oh,J. and Jeong,J.
  TITLE     Enterococcus faecalis JF3A-223 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Chae,J., Oh,J. and Jeong,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2022) Division of Biotechnology, Jeonbuk National
            University, Kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.0x
            Sequencing Technology :: Illumina iSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/20/2023 05:00:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,914
            CDSs (total)                      :: 2,836
            Genes (coding)                    :: 2,781
            CDSs (with protein)               :: 2,781
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 17 of 55
            Pseudo Genes (incomplete)         :: 34 of 55
            Pseudo Genes (internal stop)      :: 9 of 55
            Pseudo Genes (multiple problems)  :: 5 of 55
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102069.1.
            Bacteria and source DNA available from Bacteria.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="JF3A-223"
                     /isolation_source="Rectal swab"
                     /host="Pig"
                     /db_xref="taxon:1351"
                     /plasmid="pJF3A-223-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2020"
     gene            join(3108..3788,1..195)
                     /locus_tag="NP947_RS14520"
     CDS             join(3108..3788,1..195)
                     /locus_tag="NP947_RS14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_129187473.1"
                     /translation="MKKLLSGLFGVSVILMLGACANSTSGKSENTKSAAEGKFVVSKNI
                     EITEQEKEMNVTERMNELFNAVNKDTIIANTSEKFGYQFAIEENLNEPYKLSDQINKNK
                     ELIFKNLDKEIGLENYYTKTALKHIKEEFERMNQHTPFQYPIDFFFLAQYTGHYWGEVK
                     TEDNGYVNAYIDRNTIVFDGQSWKINRIDSEGENVAKIEVTNDNIRPTNNSYTFDSSIL
                     ESQIKNKESDPIAESLQLNDHKYQNMSVIFSNPAINSLQLSNMSGKPALVVTLNKEDSI
                     WKINTVEYGY"
                     /name="hypothetical protein"
     gene            complement(834..1097)
                     /locus_tag="NP947_RS14525"
     CDS             complement(834..1097)
                     /locus_tag="NP947_RS14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epsilon-antitoxin"
                     /protein_id="WP_002336758.1"
                     /translation="MTLDYRKTFEIEIINEFQSAIHSKMLNYVLNNELDKSDSTNLQVN
                     LLKQLSNMNQINLFELSLEELEAYHDYLRTIKKYADNITRTA"
                     /name="epsilon-antitoxin"
     gene            1932..2906
                     /locus_tag="NP947_RS14530"
     CDS             1932..2906
                     /locus_tag="NP947_RS14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358693.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_089202028.1"
                     /translation="MANEIVKYHSELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANNRFIDDLQDTYEKILSLRFGSRSESGLNRSMFVMFTEFEIVGD
                     VDEPYVDIRIYEKALPLLNNLDSWTRYSLQQFNELQSSYAKTMFRLLKQFRTKGCAYFS
                     KDDFYELLDIPKTYKQGNVDQKVLQPIKQELTAIFKGLTIKKKYGKGRGKPVIGYQFSF
                     KPEIKKADDFNKHAPTKEEQNRALAQADKQKEYEQQPTTIDEKILEAKRKVELLEQLKE
                     LEKKEKQDTTNQTPTEQKKSKSDSQLSEKDKQDLLNGLSNFGK"
                     /name="replication initiation protein"
     Typing          1933..2906
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacaaagaaa gtgatccaat agctgaatca ttacaattaa atgatcacaa gtaccaaaat
       61 atgtctgtca ttttcagcaa tcctgcaatt aattcgttac agttatctaa tatgagtgga
      121 aaacctgctt tggttgtaac tttaaataaa gaagattcaa tttggaaaat caacacagta
      181 gagtatggat attaattttg acaggtgaat attatgaata aagtactaaa tggactttca
      241 aattttggaa aataatttca ctgatttttt caaaaatggg gattggggca acgcccaaaa
      301 aataaaaaga atcgtctgaa acgaggaaca aattaaaatg caaattttag ttgtgaccga
      361 gtggaagatg attacttttt accgtaagtg tgtacacact tactaagctc gctacaaaga
      421 aatatctatc tgcatagaaa caagagcgta gcgaatttta acaaacacgc ttgacgggaa
      481 aagagtacag tttcacgtga gtgatctgta ctctttttta gcacgataga caccaaaata
      541 ttttggtggg tgtaagtgcg cccttagcac aacgtagaaa aaattcggta gattttttta
      601 ctgcgtataa gtgcgccctt agcacaacgt agaaaaaatt cggtagattt ttttactgcg
      661 tataagtgcg cccttagtaa aaatcactaa gggaatattt ttcaaattaa ctttgtagaa
      721 acgcacccct tatttttaag ggcgcactta tacccctaaa tttcatttag tggtaattgt
      781 gctataaaac ttaatttaca tttcgtttta tcgtcaagcg tgttggttaa aactcacgct
      841 gttcgtgtga tgttatcggc atattttttt atcgttcgta aatagtcgtg ataggcttct
      901 aattcttcta aagataattc aaataaatta atttggttca tgtttgataa ttgttttaat
      961 aaatttactt gtaggtttgt cgaatcactt ttatcgagtt cattatttaa aacatagttc
     1021 aacatttttg agtgaattgc tgattgaaat tcattgatta tctcaatttc aaatgttttt
     1081 ctgtaatcta atgtcatgtt gtttgctcct ttctggtttc gctttgctca actaaacatt
     1141 tacttttaat taaacagact tcgctagttt aatcaaaagt aaacgtgcga ataaaaagca
     1201 aatcattttt aaagctcata aaatgcgttc taaggcgttt taacgatcat gccgataaac
     1261 taactaaata aacactaaaa cctcttaaaa cgcaaaatat gacgtttaaa tggtatttta
     1321 gtttgtattg ctatctaaaa tctgcttttg ggtcgttagg gcttgccctg accgtctgaa
     1381 agacgcttga tgaacaagat tacctaaaac cactaaaaac gtcttagaga gccttatatg
     1441 gcgttctaaa cctaaaccaa tccgcctttg aacaacggtt ttgattttgc ccttagcgag
     1501 tgaacgaagt gagcgctgca agtaaaatgt gagcgtggtt ttcgatcact ccttttttgt
     1561 cttttggttt tttatttgca gttttgcaaa attcgcgaac ctgtttatct aaaaaaatga
     1621 gcttttttag gcgggaaatt ttgccccccc tcttttataa tctcttttta aacctctttt
     1681 aaacctcttt accgtcggct gtgtaattac tctcccaaga ggtacagagt cttttgaaca
     1741 accaattgtc tgtttatgaa caaccaattg tctgtttatg aacaaccaat tgtctgttta
     1801 tgaacaacca attgtctgtt tggtattgtt ttacgaacaa ccagttgtta taataagttg
     1861 tataaacaga caaaacaacc tttaaaaggt tctgtatccc ttgggagagt aaggaaagga
     1921 ctgattcata tatggctaat gagattgtta agtaccattc agaactaaat acgattcctt
     1981 tacggaaatt tactcccgta gaaatgaatt tatttttttc tatcgtgtcc agaatgcgtg
     2041 aaaaaggtga taaaaccgtt cgttttactt ttgaccaact gaaagattta agtaattaca
     2101 aagcaaccgc taacaaccgt tttattgacg acttacaaga tacttatgaa aaaattcttt
     2161 ccttgcgttt tggtagtcgt agtgaaagtg ggttaaacag aagtatgttt gtcatgttta
     2221 ctgaatttga gattgtcggg gatgttgatg aaccttacgt agatatacga atttacgaaa
     2281 aagcattgcc tttgttaaat aatcttgatt catggactcg ttactctctg caacaattca
     2341 acgagctaca aagtagttat gcaaaaacca tgttcagact gttaaaacag tttcgtacca
     2401 aaggatgtgc gtatttctct aaagacgatt tttacgaatt attggacatt ccaaaaactt
     2461 acaaacaagg taatgtcgat caaaaagttc tccagcctat caaacaagag ttaactgcaa
     2521 tttttaaagg actgacaatc aaaaagaaat acggcaaagg tcgtggcaag cctgttattg
     2581 gctatcaatt ttcgttcaaa ccagaaatca agaaagcaga cgatttcaac aaacatgcac
     2641 caaccaaaga agaacaaaat agagcgttag cacaagcgga taaacaaaaa gaatacgagc
     2701 aacaaccaac aacgattgac gagaaaattt tagaagcaaa aagaaaagtt gagctacttg
     2761 aacaactcaa agagttagaa aaaaaagaaa aacaagacac aacaaatcag acacctacag
     2821 aacaaaaaaa atcaaaaagt gattcgcaac tttccgaaaa agacaaacaa gatttactaa
     2881 atggactttc aaattttgga aaataatttc actgattttt tcaaaaatgg ggattggggc
     2941 aacgccccaa aaataaaaag aatcgtctga aacgaggaac aaattaaaat gcaaatttta
     3001 tgtaacgatc actccttatg aaatgtagga aaatgtcagc atcccatttg ttataatagt
     3061 tcttggatta aataatatat atacatagac aaaaaggaga agaatagatg aagaaattgt
     3121 taagtgggtt gtttggagta tcggtgattt taatgttggg ggcctgtgca aattcaacaa
     3181 gcggcaaaag tgaaaacacc aagagtgctg cagaaggaaa atttgttgta tcaaaaaata
     3241 tagaaataac tgaacaagaa aaagaaatga atgttactga gaggatgaat gaactattta
     3301 atgcagttaa taaagacacg ataatagcaa atacttcgga aaaatttggc tatcaatttg
     3361 ctattgaaga gaatttgaat gaaccttata aactaagtga tcaaattaat aagaataaag
     3421 aacttatttt caaaaactta gataaagaaa tagggttgga aaattattat acaaaaacag
     3481 cattgaaaca tatcaaggaa gaatttgaaa gaatgaatca acacacacct tttcaatatc
     3541 cgatagactt ctttttctta gctcaataca ctggacatta ttggggagaa gtaaagactg
     3601 aagacaatgg ttacgtgaat gcttatattg atagaaatac tatagttttt gatggtcaaa
     3661 gttggaagat aaatcgcata gattctgaag gagagaacgt tgctaaaata gaagttacaa
     3721 acgataatat tcgtccaaca aacaattcgt atacatttga ctcttctatc ttagagagtc
     3781 aaataaaa
//
